CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats. The name was minted at a time when the origin and use of the interspacing subsequences were not known. At that time the CRISPRs were described as segments of prokaryotic DNA containing short, repetitive base sequences. In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated) genes are located next to CRISPR sequences.
With the fast progress and deeper understanding of CAR-T cell therapy, finding a better solution other than lentivirus transduction is more requested, to overcome the functional limitation o...
This talk will provide an overview of our work in the design of self-assembled, functionalised peptide and protein nanoparticles, hydrogels and bio-interfaces for applications in healthcare....
Coronavirus Disease 2019 (COVID-19) has driven the use of nucleic acid testing technology in clinical testing. There is a growing need for point-of-care testing (POCT)......
In the past decade, gene editing has been increasingly emphasized as an essential tool for therapy development. This has led to significant scientific advances in understanding disease mecha...
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We present programmable ad...
Rare diseases pose unique challenges in the medical field, often affecting a small but vulnerable population with limited treatment options. The Rare Disease Challenge (RaDiChal) is a ground...
Smartphone-based optical imaging and sensing devices are among the next-generation biosensors that have shown great potential to transform the field of point-of-care (POC) diagnostics....
In this talk, I will introduce two new tools that we developed based on CRISPR-enabled gene regulation in bacteria, and one method based on P1 phagemid-enabled delivery of Cas9 nuclease. Fir...
CRISPR-based diagnostics offer rapid and highly sensitive detection of disease biomarkers. Their ease of use and compatibility with lateral-flow readouts make them suitable for point-of-care...
Endonuclease Cas12a, as one key part of the CRISPR/Cas biotechnology, possessed a unique dual enzymatic function which has been widely used for various applications1. However, the limits tra...
Molecular diagnostics based on clustered regularly interspaced short palindromic repeats (CRISPR) enzyme systems have been the subject of intense research....
Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindr...
With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
Join us for an educational webinar that delves into the fascinating world of Extracellular Vesicles (EVs) and their impact on biomedical research and medicine. In collaboration with ExoVecto...
Inefficient knock-in of transgene cargos limits the potential of cell-based medicines. In this study, we used a CRISPR nuclease that targets a site within an exon of an essential gene and de...
Employing a metagenomic search, we identified a family of miniature CRISPR-Cas type V-L systems capable of RNA-guided dsDNA target cleavage without a tracrRNA. A bacterial depletion screen r...
Phosphorylation of α-synuclein at the Serine-129 site (α-syn Ser129P) is an established pathologic hallmark of synucleinopathies and a therapeutic target. In physiologic states,...
Genetically modified cell models accelerate the development of safe and effective therapeutics, making them indispensable in the quest for new drugs. Through manipulation of cell line geneti...
Characterization of rare cells remains a major challenge for the evaluation and understanding of key biological systems, such as circulating tumor cells (CTCs) from liquid biopsy, stem cells...
Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
Date: March 08, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Engineered nucleases like the CRISPR-Cas9 system provide researchers with powerful tools for the manipulation of the geno...
With the fast progress and deeper understanding of CAR-T cell therapy, finding a better solution other than lentivirus transduction is more requested, to overcome the functional limitation o...
This talk will provide an overview of our work in the design of self-assembled, functionalised peptide and protein nanoparticles, hydrogels and bio-interfaces for applications in healthcare....
Coronavirus Disease 2019 (COVID-19) has driven the use of nucleic acid testing technology in clinical testing. There is a growing need for point-of-care testing (POCT)......
In the past decade, gene editing has been increasingly emphasized as an essential tool for therapy development. This has led to significant scientific advances in understanding disease mecha...
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We present programmable ad...
Rare diseases pose unique challenges in the medical field, often affecting a small but vulnerable population with limited treatment options. The Rare Disease Challenge (RaDiChal) is a ground...
Smartphone-based optical imaging and sensing devices are among the next-generation biosensors that have shown great potential to transform the field of point-of-care (POC) diagnostics....
In this talk, I will introduce two new tools that we developed based on CRISPR-enabled gene regulation in bacteria, and one method based on P1 phagemid-enabled delivery of Cas9 nuclease. Fir...
CRISPR-based diagnostics offer rapid and highly sensitive detection of disease biomarkers. Their ease of use and compatibility with lateral-flow readouts make them suitable for point-of-care...
Endonuclease Cas12a, as one key part of the CRISPR/Cas biotechnology, possessed a unique dual enzymatic function which has been widely used for various applications1. However, the limits tra...
Molecular diagnostics based on clustered regularly interspaced short palindromic repeats (CRISPR) enzyme systems have been the subject of intense research....
Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindr...
With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
Join us for an educational webinar that delves into the fascinating world of Extracellular Vesicles (EVs) and their impact on biomedical research and medicine. In collaboration with ExoVecto...
Inefficient knock-in of transgene cargos limits the potential of cell-based medicines. In this study, we used a CRISPR nuclease that targets a site within an exon of an essential gene and de...
Employing a metagenomic search, we identified a family of miniature CRISPR-Cas type V-L systems capable of RNA-guided dsDNA target cleavage without a tracrRNA. A bacterial depletion screen r...
Phosphorylation of α-synuclein at the Serine-129 site (α-syn Ser129P) is an established pathologic hallmark of synucleinopathies and a therapeutic target. In physiologic states,...
Genetically modified cell models accelerate the development of safe and effective therapeutics, making them indispensable in the quest for new drugs. Through manipulation of cell line geneti...
Characterization of rare cells remains a major challenge for the evaluation and understanding of key biological systems, such as circulating tumor cells (CTCs) from liquid biopsy, stem cells...
Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
Date: March 08, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Engineered nucleases like the CRISPR-Cas9 system provide researchers with powerful tools for the manipulation of the geno...
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