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    Library Preparation

    Library preparation is the initial part of the procedure for next generation sequencing. During this step, DNA or RNA is fragmented and then adhered, or ligated, to the sequencing platform allowing for sample identification. In ligation-based library prep fragmentation and ligation occur in two separate steps while in tagmentation-based library prep fragmentation and ligation are completed in one step.

    Webinars

    • MAY 05, 2021 | 12:00 PM
      How to improve seized drug analysis workflows and throughput with fast, specific analysis by direct ionisation mass spectrometry?
      How to improve seized drug analysis workflows and throughput with...
      The increase in the number and diversity of drugs of abuse is a major concern; it also presents significant challenges for forensic laboratories who are involved in the analysis of seized su...
      Speaker: Francis Diamond
      Presented at: Forensic Sciences Virtual Event Series 2021
      Sponsored By: Waters (Forensic Sciences)
      2
      APR 20, 2021 | 4:30 PM
      Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene Therapy Research
      Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene...
      Interest in cell and gene therapy-based disease prevention and treatment has increased rapidly over the last few decades, however there are still many hurdles to overcome and further progres...
      Speaker: Andrea O'Hara, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      Sponsored By: GENEWIZ
      3
      APR 20, 2021 | 6:00 AM
      Panel Presentation: Accelerating SARS-CoV-2 genomic surveillance with a new high-throughput NGS solution
      Panel Presentation: Accelerating SARS-CoV-2 genomic surveillance...
      Since late 2020, several prominent SARS-CoV-2 variants of concern have emerged harboring specific mutations which increase viral transmissibility (e.g., lineage B.1.1.7), and which appear to...
      Speaker: Brian Dugan, M.S. , Fernando Spikli, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      Sponsored By: QIAGEN
      1
      APR 20, 2021 | 12:00 AM
      Defining Cellular Heterogeneity and Cell-Type Specific Pathogenesis in the Retina Using Single Cell RNA-Sequencing
      Defining Cellular Heterogeneity and Cell-Type Specific Pathogenesis...
      The ability of single-cell RNA-Seq (scRNA-Seq) to measure the transcriptional basis of heterogeneity between individual cells is transforming biomedical research. It enables the precise &lsq...
      Speaker: David Simpson, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      1
      DEC 15, 2020 | 10:00 AM
      Prep for Success: From human microbiome exploration to advanced SARS-CoV-2 testing in wastewater
      Prep for Success: From human microbiome exploration to advanced...
      DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
      Speaker: Sasha Vlassov, PhD , Jasti Madhu , Emily Zeringer
      Sponsored By: Thermo Fisher Scientific
      13
      DEC 03, 2020 | 4:30 PM
      An Iterative Approach to Structure-based in silico to in vivo Development of Combinatorial Anti-SARS-CoV-2 Therapy
      An Iterative Approach to Structure-based in silico to in vivo...
      This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
      Speaker: Garth Ehrlich, PhD, FAAAS
      Presented at: Coronavirus Series
      5
      JUL 02, 2020 | 12:45 PM
      Strategies to normalize miRNA expression in serum and plasma
      Strategies to normalize miRNA expression in serum and plasma
      MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post transcriptional level. They are known to play a critical role in multiple biologi...
      Speaker: Emily Zeringer , Harita Veereshlingam, PhD
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      2
      MAY 19, 2020 | 8:00 AM
      Looking Beyond PCR: Isothermal Amplification
      Looking Beyond PCR: Isothermal Amplification
      DATE: May 19, 2020 TIME: 8:00am PT, 11:00am ET Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a vari...
      Speaker: Agne Alminaite, PhD , Remigijus Skirgaila, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      71 5
      MAY 07, 2020 | 7:00 AM
      A complete workflow for analysis of cfDNA: from plasma to variants
      A complete workflow for analysis of cfDNA: from plasma to variants
      DATE: May 7, 2020 TIME: 7:00am PT As cost of sequencing continues to drop, the throughput and complexity of NGS assays have risen precipitously. At the same time, the types of samples being...
      Speaker: Shilpa Parakh , Nicole Roseman
      Sponsored By: Beckman Coulter Life Sciences, IDT
      13 10
      DEC 04, 2019 | 7:00 AM
      Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
      Improve your reproducibility - Liquid Handling is a crucial factor...
      DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
      Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
      Sponsored By: Eppendorf
      12
      JUN 20, 2019 | 1:30 PM
      Single Cell CNV End-to-End Workflow to Dissect Tumor Heterogeneity
      Single Cell CNV End-to-End Workflow to Dissect Tumor Heterogeneity
      Tumor heterogeneity is a hallmark of cancer and can have significant impact on identifying drivers, including those that may be therapeutically relevant. Although, the traditional sequencing...
      Speaker: Lee Gibbs, PhD
      Presented at: Precision Medicine Virtual Event Series 2019
      1
      JUN 20, 2019 | 10:30 AM
      The Massively Parallel Sequencing (MPS) Revolution
      The Massively Parallel Sequencing (MPS) Revolution
      The development of automated DNA sequencers utilizing Sanger sequencing and capillary electrophoresis made it possible to develop the first draft sequences of the human genome. The cost of do...
      Speaker: David Smith, PhD
      Presented at: Precision Medicine Virtual Event Series 2019
      1
      MAY 16, 2019 | 4:00 PM
      Towards Standardized Workflows - A focus on microbiome insights
      Towards Standardized Workflows - A focus on microbiome insights
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
      Sponsored By: Eppendorf
      10 2
      MAR 05, 2019 | 9:00 AM
      Strategies to normalize miRNA expression in serum and plasma.
      Strategies to normalize miRNA expression in serum and plasma.
      DATE:  March 5, 2019TIME:  09:00am PST, 12:00pm EST MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post tran...
      Speaker: Emily Zeringer , Harita Veereshlingam, PhD
      Sponsored By: Thermo Fisher Scientific - Applied Biosystems
      7
      OCT 10, 2018 | 12:00 PM
      Sample to Insight:  Everything You Need to Know About RNA Sequencing
      Sample to Insight: Everything You Need to Know About RNA Sequencing
      RNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving hig...
      Speaker: Jonathan Shaffer, PhD, MBA
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      MAY 09, 2018 | 9:00 AM
      Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
      Gene Expression Analysis - Critical Factors for Overcoming Challenges...
      Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
      Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      APR 26, 2018 | 7:30 AM
      Switching from manual to automated NGS library preparation
      Switching from manual to automated NGS library preparation
      DATE: April 26, 2018TIME: 07:30am PDT, 04:30pm CESTThe NGS library preparation process is labor-intensive, requiring experience and attention to detail over hours. Eppendorf consumables...
      Speaker: Tim Schommartz, PhD , Marc-Manuel Hahn, PhD
      Sponsored By: Eppendorf
      8 1
      OCT 12, 2017 | 9:00 AM
      Circulating biomarkers - what matters for the isolation of biomarkers from Liquid Biopsy samples
      Circulating biomarkers - what matters for the isolation of biomarkers...
      The analysis of circulating biomarkers in a patient’s blood holds significant potential for early disease detection and disease monitoring. Circulating DNA is the most commonly studied...
      Speaker: Verena Schramm, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
      Sponsored By: QIAGEN Molecular Diagnostics
      1
      MAY 25, 2017 | 6:00 AM
      In-House Technology Development for Next Gen Sequencing, Tissue Handling and beyond, at the BC Cancer Agency
      In-House Technology Development for Next Gen Sequencing, Tissue...
      The Instrumentation Group at the BC Cancer Agency’s Genome Sciences Centre (GSC) is unusual in having an engineering group and extensive prototyping facilities embedded in a biomedical...
      Speaker: Robin Coope
      Presented at: Lab Automation Virtual Event Series 2017
      2
      MAY 10, 2017 | 7:30 AM
      C.E. CREDITS
      Enrichment of cffDNA and ctDNA via size selection for reduced diagnostic assay costs
      Enrichment of cffDNA and ctDNA via size selection for reduced...
      Cell free DNA (cfDNA) analysis for the purpose of diagnostics is currently practiced for non-invasive prenatal screening of cell free fetal DNA (cffDNA), and is anticipated to be leveraged fo...
      Speaker: Matthew Nesbitt, MSc
      Presented at: Genetics and Genomics Virtual Event Series 2017
      1
      MAY 10, 2017 | 6:00 AM
      C.E. CREDITS
      CATS - a novel library preparation method for ultrasensitive deep sequencing of extracellular circulating RNA and DNA
      CATS - a novel library preparation method for ultrasensitive deep...
      Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of nucleic acids still rem...
      Speaker: Andrey Turchinovich, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2017
      Sponsored By: Diagenode
      4
      APR 20, 2017 | 7:30 AM
      Advances in Cellular Automation
      Advances in Cellular Automation
      DATE: April 20, 2017TIME: 7:30am PT, 10:30am ETCell culture and cellular applications have been and continue to be essential to medical and biological research. By automating these assa...
      Speaker: Mike Kowalski, Ph.D.
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences
      6
      JAN 19, 2017 | 8:00 AM
      Webinar: Detecting meaningful mutations from liquid biopsies by Anchored Multiplex PCR
      Webinar: Detecting meaningful mutations from liquid biopsies by...
      DATE: January 19th, 2017TIME: 8:00AM PT, 11:00AM ETLiquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational st...
      Speaker: Josh Stahl , Jonathan Doose
      Sponsored By: ArcherDX, ArcherDX
      9
      NOV 22, 2016 | 11:30 PM
      Laboratory automation to deliver reliable results faster
      Laboratory automation to deliver reliable results faster
      DATE: November 23, 2016 TIME: 8:30am CET/10:30am Qatar/11:30am UAE In life science research today the trend goes to increased sample numbers, higher degree of sensitivity of d...
      Speaker: Stefan Overkamp, PhD
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      5 3
    MAY 05, 2021 | 12:00 PM
    How to improve seized drug analysis workflows and throughput with fast, specific analysis by direct ionisation mass spectrometry?
    How to improve seized drug analysis workflows and throughput with...
    The increase in the number and diversity of drugs of abuse is a major concern; it also presents significant challenges for forensic laboratories who are involved in the analysis of seized su...
    Speaker: Francis Diamond
    Presented at: Forensic Sciences Virtual Event Series 2021
    Sponsored By: Waters (Forensic Sciences)
    2
    APR 20, 2021 | 4:30 PM
    Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene Therapy Research
    Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene...
    Interest in cell and gene therapy-based disease prevention and treatment has increased rapidly over the last few decades, however there are still many hurdles to overcome and further progres...
    Speaker: Andrea O'Hara, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    Sponsored By: GENEWIZ
    3
    APR 20, 2021 | 6:00 AM
    Panel Presentation: Accelerating SARS-CoV-2 genomic surveillance with a new high-throughput NGS solution
    Panel Presentation: Accelerating SARS-CoV-2 genomic surveillance...
    Since late 2020, several prominent SARS-CoV-2 variants of concern have emerged harboring specific mutations which increase viral transmissibility (e.g., lineage B.1.1.7), and which appear to...
    Speaker: Brian Dugan, M.S. , Fernando Spikli, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    Sponsored By: QIAGEN
    1
    APR 20, 2021 | 12:00 AM
    Defining Cellular Heterogeneity and Cell-Type Specific Pathogenesis in the Retina Using Single Cell RNA-Sequencing
    Defining Cellular Heterogeneity and Cell-Type Specific Pathogenesis...
    The ability of single-cell RNA-Seq (scRNA-Seq) to measure the transcriptional basis of heterogeneity between individual cells is transforming biomedical research. It enables the precise &lsq...
    Speaker: David Simpson, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    1
    DEC 15, 2020 | 10:00 AM
    Prep for Success: From human microbiome exploration to advanced SARS-CoV-2 testing in wastewater
    Prep for Success: From human microbiome exploration to advanced...
    DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
    Speaker: Sasha Vlassov, PhD , Jasti Madhu , Emily Zeringer
    Sponsored By: Thermo Fisher Scientific
    13
     
     
  • DEC 03, 2020 | 4:30 PM
    An Iterative Approach to Structure-based in silico to in vivo Development of Combinatorial Anti-SARS-CoV-2 Therapy
    An Iterative Approach to Structure-based in silico to in vivo...
    This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
    Speaker: Garth Ehrlich, PhD, FAAAS
    Presented at: Coronavirus Series
    5
    JUL 02, 2020 | 12:45 PM
    Strategies to normalize miRNA expression in serum and plasma
    Strategies to normalize miRNA expression in serum and plasma
    MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post transcriptional level. They are known to play a critical role in multiple biologi...
    Speaker: Emily Zeringer , Harita Veereshlingam, PhD
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    2
    MAY 19, 2020 | 8:00 AM
    Looking Beyond PCR: Isothermal Amplification
    Looking Beyond PCR: Isothermal Amplification
    DATE: May 19, 2020 TIME: 8:00am PT, 11:00am ET Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a vari...
    Speaker: Agne Alminaite, PhD , Remigijus Skirgaila, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    71 5
    MAY 07, 2020 | 7:00 AM
    A complete workflow for analysis of cfDNA: from plasma to variants
    A complete workflow for analysis of cfDNA: from plasma to variants
    DATE: May 7, 2020 TIME: 7:00am PT As cost of sequencing continues to drop, the throughput and complexity of NGS assays have risen precipitously. At the same time, the types of samples being...
    Speaker: Shilpa Parakh , Nicole Roseman
    Sponsored By: Beckman Coulter Life Sciences, IDT
    13 10
    DEC 04, 2019 | 7:00 AM
    Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
    Improve your reproducibility - Liquid Handling is a crucial factor...
    DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
    Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
    Sponsored By: Eppendorf
    12
     
     
  • JUN 20, 2019 | 1:30 PM
    Single Cell CNV End-to-End Workflow to Dissect Tumor Heterogeneity
    Single Cell CNV End-to-End Workflow to Dissect Tumor Heterogeneity
    Tumor heterogeneity is a hallmark of cancer and can have significant impact on identifying drivers, including those that may be therapeutically relevant. Although, the traditional sequencing...
    Speaker: Lee Gibbs, PhD
    Presented at: Precision Medicine Virtual Event Series 2019
    1
    JUN 20, 2019 | 10:30 AM
    The Massively Parallel Sequencing (MPS) Revolution
    The Massively Parallel Sequencing (MPS) Revolution
    The development of automated DNA sequencers utilizing Sanger sequencing and capillary electrophoresis made it possible to develop the first draft sequences of the human genome. The cost of do...
    Speaker: David Smith, PhD
    Presented at: Precision Medicine Virtual Event Series 2019
    1
    MAY 16, 2019 | 4:00 PM
    Towards Standardized Workflows - A focus on microbiome insights
    Towards Standardized Workflows - A focus on microbiome insights
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
    Sponsored By: Eppendorf
    10 2
    MAR 05, 2019 | 9:00 AM
    Strategies to normalize miRNA expression in serum and plasma.
    Strategies to normalize miRNA expression in serum and plasma.
    DATE:  March 5, 2019TIME:  09:00am PST, 12:00pm EST MicroRNA(miRNA) are short non-coding single stranded RNA molecules that regulate gene expression at the post tran...
    Speaker: Emily Zeringer , Harita Veereshlingam, PhD
    Sponsored By: Thermo Fisher Scientific - Applied Biosystems
    7
    OCT 10, 2018 | 12:00 PM
    Sample to Insight:  Everything You Need to Know About RNA Sequencing
    Sample to Insight: Everything You Need to Know About RNA Sequencing
    RNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving hig...
    Speaker: Jonathan Shaffer, PhD, MBA
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
     
     
  • MAY 09, 2018 | 9:00 AM
    Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
    Gene Expression Analysis - Critical Factors for Overcoming Challenges...
    Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
    Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
    APR 26, 2018 | 7:30 AM
    Switching from manual to automated NGS library preparation
    Switching from manual to automated NGS library preparation
    DATE: April 26, 2018TIME: 07:30am PDT, 04:30pm CESTThe NGS library preparation process is labor-intensive, requiring experience and attention to detail over hours. Eppendorf consumables...
    Speaker: Tim Schommartz, PhD , Marc-Manuel Hahn, PhD
    Sponsored By: Eppendorf
    8 1
    OCT 12, 2017 | 9:00 AM
    Circulating biomarkers - what matters for the isolation of biomarkers from Liquid Biopsy samples
    Circulating biomarkers - what matters for the isolation of biomarkers...
    The analysis of circulating biomarkers in a patient’s blood holds significant potential for early disease detection and disease monitoring. Circulating DNA is the most commonly studied...
    Speaker: Verena Schramm, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
    Sponsored By: QIAGEN Molecular Diagnostics
    1
    MAY 25, 2017 | 6:00 AM
    In-House Technology Development for Next Gen Sequencing, Tissue Handling and beyond, at the BC Cancer Agency
    In-House Technology Development for Next Gen Sequencing, Tissue...
    The Instrumentation Group at the BC Cancer Agency’s Genome Sciences Centre (GSC) is unusual in having an engineering group and extensive prototyping facilities embedded in a biomedical...
    Speaker: Robin Coope
    Presented at: Lab Automation Virtual Event Series 2017
    2
    MAY 10, 2017 | 7:30 AM
    C.E. CREDITS
    Enrichment of cffDNA and ctDNA via size selection for reduced diagnostic assay costs
    Enrichment of cffDNA and ctDNA via size selection for reduced...
    Cell free DNA (cfDNA) analysis for the purpose of diagnostics is currently practiced for non-invasive prenatal screening of cell free fetal DNA (cffDNA), and is anticipated to be leveraged fo...
    Speaker: Matthew Nesbitt, MSc
    Presented at: Genetics and Genomics Virtual Event Series 2017
    1
     
     
  • MAY 10, 2017 | 6:00 AM
    C.E. CREDITS
    CATS - a novel library preparation method for ultrasensitive deep sequencing of extracellular circulating RNA and DNA
    CATS - a novel library preparation method for ultrasensitive deep...
    Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of nucleic acids still rem...
    Speaker: Andrey Turchinovich, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2017
    Sponsored By: Diagenode
    4
    APR 20, 2017 | 7:30 AM
    Advances in Cellular Automation
    Advances in Cellular Automation
    DATE: April 20, 2017TIME: 7:30am PT, 10:30am ETCell culture and cellular applications have been and continue to be essential to medical and biological research. By automating these assa...
    Speaker: Mike Kowalski, Ph.D.
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences
    6
    JAN 19, 2017 | 8:00 AM
    Webinar: Detecting meaningful mutations from liquid biopsies by Anchored Multiplex PCR
    Webinar: Detecting meaningful mutations from liquid biopsies by...
    DATE: January 19th, 2017TIME: 8:00AM PT, 11:00AM ETLiquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational st...
    Speaker: Josh Stahl , Jonathan Doose
    Sponsored By: ArcherDX, ArcherDX
    9
    NOV 22, 2016 | 11:30 PM
    Laboratory automation to deliver reliable results faster
    Laboratory automation to deliver reliable results faster
    DATE: November 23, 2016 TIME: 8:30am CET/10:30am Qatar/11:30am UAE In life science research today the trend goes to increased sample numbers, higher degree of sensitivity of d...
    Speaker: Stefan Overkamp, PhD
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    5 3
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