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    Library Preparation

    Library preparation is the initial part of the procedure for next generation sequencing. During this step, DNA or RNA is fragmented and then adhered, or ligated, to the sequencing platform allowing for sample identification. In ligation-based library prep fragmentation and ligation occur in two separate steps while in tagmentation-based library prep fragmentation and ligation are completed in one step.

    Webinars

    • NOV 03, 2016 | 6:00 AM
      Mutation Detection Simplified: From Liquid Biopsy in clinical oncology to the detection of Drug Resistance Associated Mutations in virology
      Mutation Detection Simplified: From Liquid Biopsy in clinical...
      Sanger sequencing and polymerase chain reaction (PCR) methods have been the standard molecular methods in clinical diagnostics for decades.  Next-Generation Sequencing (NGS) technology r...
      Speaker: Gerd Michel, PhD , Lou Welebob
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      5
      SEP 07, 2016 | 6:00 AM
      MinION™ Applications: Microbial genomics and direct RNA sequencing
      MinION™ Applications: Microbial genomics and direct RNA sequencing
      Oxford Nanopore’s MinION is a small sensing device which can sequence DNA and RNA directly, without the need to perform an enzymatic synthesis reaction. The device is portable and is po...
      Speaker: Daniel Turner, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2016
      2
      JUL 13, 2016 | 8:00 PM
      Easy Automation Solutions. Better Results.
      Easy Automation Solutions. Better Results.
      This presentation is a rebroadcast of a previous webinar with live Q&A. Please confirm the start time and date in your time zone. We’ve included several examples below for your re...
      Speaker: David Horvath, MS , Zach Smith, MS
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      2 1
      JUN 23, 2016 | 8:00 AM
      DNA & RNA Sequencing Sample Prep: Automating Simple to Complex Methods
      DNA & RNA Sequencing Sample Prep: Automating Simple to Complex...
      DNA and RNA sequencing sample preparation can involve many tedious and error prone steps. Liquid handling automation solutions can help standardize your processes and achieve more consistent ...
      Speaker: David Horvath, MS , Zach Smith, MS
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      3
      JUN 22, 2016 | 9:00 AM
      Mapping nuclear-exosome targeted poly(A) tails with 3´-RNA seq
      Mapping nuclear-exosome targeted poly(A) tails with 3´-RNA seq
      DATE: June 22, 2016 TIME: 9am Pacific time, 12pm Eastern time, 6pm Central European time A large fraction of the RNA transcribed in eukaryotic cells is rapidly degraded in the nucleus. A ...
      Speaker: Kevin Roy, PhD
      Sponsored By: Lexogen, Lexogen
      10
      JUN 09, 2016 | 9:00 AM
      Standardizing Microbiomics
      Standardizing Microbiomics
      DATE:  June 9, 2016 TIME:   9AM Pacific time, 12pm Eastern time The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and...
      Speaker: Ryan Kemp, M.S. , Shuiquan Tang, PhD
      Sponsored By: Zymo Research, Zymo Research
      13
      MAY 19, 2016 | 8:00 AM
      WEBINAR: Easy Automation Solutions. Better Results.
      WEBINAR: Easy Automation Solutions. Better Results.
      Generating NGS data you can trust is highly reliant on the quality of the library sample that’s sequenced.  NGS sample prep has become more streamlined, but it’s still a labo...
      Speaker: David Horvath, MS , Zach Smith, MS
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      6
      MAY 11, 2016 | 6:00 AM
      Expanding the molecular toolbox for Nanopore sequencing
      Expanding the molecular toolbox for Nanopore sequencing
      Library preparation for Nanopore sequencing is a very flexible process. There are many ways in which a sequencing template, or other analyte, can be presented to a nanopore for analysis. PCR...
      Speaker: David Stoddart, MBioch, DPhil
      Presented at: Genetics and Genomics Virtual Event Series 2016
      5
      APR 04, 2016 | 8:00 AM
      Phage Antibody Selection on the Reichert SR7500DC SPR System
      Phage Antibody Selection on the Reichert SR7500DC SPR System
      DATE: April 4, 2016 TIME:  8:00am Pacific time, 11:00am Eastern time The use of surface plasmon resonance to characterize protein:protein interactions is well established and has bee...
      Speaker: Mark Sullivan, Ph.D.
      Sponsored By: Ametek, Ametek
      9
      MAR 15, 2016 | 10:00 AM
      Gene Expression Analysis Using 3'-RNA Sequencing
      Gene Expression Analysis Using 3'-RNA Sequencing
      10:00AM PT, 12:00PM CT, 1:00PM ET RNA sequencing (RNA-seq) has revolutionized the study of gene expression in animals, plants and microorganisms. However, because of its high cost, this te...
      Speaker: Behnam Abasht, PhD
      Sponsored By: Lexogen, Lexogen
      15 1
      NOV 12, 2015 | 1:30 PM
      NGS in clinical research
      NGS in clinical research
      Currently, next-generation sequencing is not only being used for basic discovery research but also in clinical settings for translational biomarker profiling and clinical research studies inc...
      Speaker: Jennifer Fostel, MS
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      1
      SEP 16, 2015 | 5:00 AM
      Synthetic Chemists to Aid the Community for Open Antimicrobial Drug Discovery
      Synthetic Chemists to Aid the Community for Open Antimicrobial...
      The antibiotic pipeline is broken, with a dearth of new antibiotics, a collapse in pharmaceutical company research1, and the exhaustion of chemical diversity contained in pharma libraries. "T...
      Speaker: Mark Blaskovich
      Presented at: C&EN's Virtual Symposium: Advances in Drug Discovery and Development
      2
      SEP 03, 2015 | 12:00 PM
      Applying the MinION: microbial genomics and complex metagenomics
      Applying the MinION: microbial genomics and complex metagenomics
      Oxford Nanopore's MinION is a small, portable USB-powered sensing device which is powered by nanopore technology. It is adaptable to the analysis of DNA, RNA, proteins or small molecules, and...
      Speaker: Daniel Turner, PhD
      Presented at: Microbiology Virtual Event Series 2015
      6
      JAN 27, 2015 | 2:00 AM
      Catch-and-Release: Extraction and Purification of NGS-Grade DNA and RNA from FFPE Tissue Samples
      Catch-and-Release: Extraction and Purification of NGS-Grade DNA...
      Formalin Fixed, Paraffin Embedded (FFPE) tissues are rapidly being adopted for transcriptome analysis in conjunction with targeted and whole genome sequencing. Harsh formaldehyde fixation an...
      Speaker: Guillaume Durin, PhD
      Sponsored By: Covaris, Inc., Covaris, Inc.
      10
      JAN 21, 2015 | 10:00 PM
      NGS-Quality Sample Preparation with Covaris Adaptive Focused Acoustics (AFA™)
      NGS-Quality Sample Preparation with Covaris Adaptive Focused Acoustics...
       In this presentation we will illustrate the advantages of AFA technology in clinically significant applications (NGS, FFPE, ChIP) and will demonstrate the flexibility of Covaris AFA in deliv...
      Speaker: Hamid Khoja Ph.D.
      Sponsored By: Covaris, Inc., Covaris, Inc.
      8
      AUG 21, 2014 | 11:30 AM
      Automation of High Throughput Agarose Gel Electrophoresis with the NIMBUS Select Featuring Ranger Technology
      Automation of High Throughput Agarose Gel Electrophoresis with...
      Coastal Genomics and the Hamilton Company have teamed up to combine the NIMBUS 96 channel platform with Ranger Technology to automate high throughput agarose gel electrophoresis. The new NIM...
      Speaker: Matthew Nesbitt, MSc
      Presented at: Genetics and Genomics Virtual Event Series 2014
      0
      AUG 20, 2014 | 7:30 AM
      Panel Discussion: Increasing productivity and reproducibility by automating DNA library preparation for Ion Torrent Sequencing
      Panel Discussion: Increasing productivity and reproducibility...
      Next Generation Sequencing has revolutionized genomic variant discovery. The major bottle-neck for sequencing projects is the individual, biological sample, library preparation. This platfo...
      Speaker: Joanna Hamilton, PhD, Lynne Apone, PhD, Zach Smith, MS
      Presented at: Genetics and Genomics Virtual Event Series 2014
      12
      OCT 17, 2013 | 7:00 AM
      Advancing Translational Cancer Research through Automated RNASeq Sample Preparation from Archival FFPE Tissue Specimens
      Advancing Translational Cancer Research through Automated RNASeq...
      RNA-Seq allows the simultaneous observation of gene expression levels, mutations in the coding sequences, splice variants and gene fusions, which are especially important in cancer studies. A...
      Speaker: Michaela Bowden, PhD
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2013
      98
      AUG 21, 2013 | 11:00 AM
      Panel Discussion: Meeting the Demand of Increased NGS Capacity with Automated TruSeq Stranded mRNA Library Preparation
      Panel Discussion: Meeting the Demand of Increased NGS Capacity...
      RNASeq has become the primary method for studying changes in gene expression in modern genomics research. By directly sequencing the various types of RNA molecules, including mRNA, ncRNA, an...
      Speaker: Alisa Jackson, Zach Smith, Mary Blair, Dave Delano
      Presented at: Genetics and Genomics Virtual Event Series 2013
      41
    NOV 03, 2016 | 6:00 AM
    Mutation Detection Simplified: From Liquid Biopsy in clinical oncology to the detection of Drug Resistance Associated Mutations in virology
    Mutation Detection Simplified: From Liquid Biopsy in clinical...
    Sanger sequencing and polymerase chain reaction (PCR) methods have been the standard molecular methods in clinical diagnostics for decades.  Next-Generation Sequencing (NGS) technology r...
    Speaker: Gerd Michel, PhD , Lou Welebob
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    5
    SEP 07, 2016 | 6:00 AM
    MinION™ Applications: Microbial genomics and direct RNA sequencing
    MinION™ Applications: Microbial genomics and direct RNA sequencing
    Oxford Nanopore’s MinION is a small sensing device which can sequence DNA and RNA directly, without the need to perform an enzymatic synthesis reaction. The device is portable and is po...
    Speaker: Daniel Turner, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2016
    2
    JUL 13, 2016 | 8:00 PM
    Easy Automation Solutions. Better Results.
    Easy Automation Solutions. Better Results.
    This presentation is a rebroadcast of a previous webinar with live Q&A. Please confirm the start time and date in your time zone. We’ve included several examples below for your re...
    Speaker: David Horvath, MS , Zach Smith, MS
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    2 1
    JUN 23, 2016 | 8:00 AM
    DNA & RNA Sequencing Sample Prep: Automating Simple to Complex Methods
    DNA & RNA Sequencing Sample Prep: Automating Simple to Complex...
    DNA and RNA sequencing sample preparation can involve many tedious and error prone steps. Liquid handling automation solutions can help standardize your processes and achieve more consistent ...
    Speaker: David Horvath, MS , Zach Smith, MS
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    3
    JUN 22, 2016 | 9:00 AM
    Mapping nuclear-exosome targeted poly(A) tails with 3´-RNA seq
    Mapping nuclear-exosome targeted poly(A) tails with 3´-RNA seq
    DATE: June 22, 2016 TIME: 9am Pacific time, 12pm Eastern time, 6pm Central European time A large fraction of the RNA transcribed in eukaryotic cells is rapidly degraded in the nucleus. A ...
    Speaker: Kevin Roy, PhD
    Sponsored By: Lexogen, Lexogen
    10
     
     
  • JUN 09, 2016 | 9:00 AM
    Standardizing Microbiomics
    Standardizing Microbiomics
    DATE:  June 9, 2016 TIME:   9AM Pacific time, 12pm Eastern time The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and...
    Speaker: Ryan Kemp, M.S. , Shuiquan Tang, PhD
    Sponsored By: Zymo Research, Zymo Research
    13
    MAY 19, 2016 | 8:00 AM
    WEBINAR: Easy Automation Solutions. Better Results.
    WEBINAR: Easy Automation Solutions. Better Results.
    Generating NGS data you can trust is highly reliant on the quality of the library sample that’s sequenced.  NGS sample prep has become more streamlined, but it’s still a labo...
    Speaker: David Horvath, MS , Zach Smith, MS
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    6
    MAY 11, 2016 | 6:00 AM
    Expanding the molecular toolbox for Nanopore sequencing
    Expanding the molecular toolbox for Nanopore sequencing
    Library preparation for Nanopore sequencing is a very flexible process. There are many ways in which a sequencing template, or other analyte, can be presented to a nanopore for analysis. PCR...
    Speaker: David Stoddart, MBioch, DPhil
    Presented at: Genetics and Genomics Virtual Event Series 2016
    5
    APR 04, 2016 | 8:00 AM
    Phage Antibody Selection on the Reichert SR7500DC SPR System
    Phage Antibody Selection on the Reichert SR7500DC SPR System
    DATE: April 4, 2016 TIME:  8:00am Pacific time, 11:00am Eastern time The use of surface plasmon resonance to characterize protein:protein interactions is well established and has bee...
    Speaker: Mark Sullivan, Ph.D.
    Sponsored By: Ametek, Ametek
    9
    MAR 15, 2016 | 10:00 AM
    Gene Expression Analysis Using 3'-RNA Sequencing
    Gene Expression Analysis Using 3'-RNA Sequencing
    10:00AM PT, 12:00PM CT, 1:00PM ET RNA sequencing (RNA-seq) has revolutionized the study of gene expression in animals, plants and microorganisms. However, because of its high cost, this te...
    Speaker: Behnam Abasht, PhD
    Sponsored By: Lexogen, Lexogen
    15 1
     
     
  • NOV 12, 2015 | 1:30 PM
    NGS in clinical research
    NGS in clinical research
    Currently, next-generation sequencing is not only being used for basic discovery research but also in clinical settings for translational biomarker profiling and clinical research studies inc...
    Speaker: Jennifer Fostel, MS
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    1
    SEP 16, 2015 | 5:00 AM
    Synthetic Chemists to Aid the Community for Open Antimicrobial Drug Discovery
    Synthetic Chemists to Aid the Community for Open Antimicrobial...
    The antibiotic pipeline is broken, with a dearth of new antibiotics, a collapse in pharmaceutical company research1, and the exhaustion of chemical diversity contained in pharma libraries. "T...
    Speaker: Mark Blaskovich
    Presented at: C&EN's Virtual Symposium: Advances in Drug Discovery and Development
    2
    SEP 03, 2015 | 12:00 PM
    Applying the MinION: microbial genomics and complex metagenomics
    Applying the MinION: microbial genomics and complex metagenomics
    Oxford Nanopore's MinION is a small, portable USB-powered sensing device which is powered by nanopore technology. It is adaptable to the analysis of DNA, RNA, proteins or small molecules, and...
    Speaker: Daniel Turner, PhD
    Presented at: Microbiology Virtual Event Series 2015
    6
    JAN 27, 2015 | 2:00 AM
    Catch-and-Release: Extraction and Purification of NGS-Grade DNA and RNA from FFPE Tissue Samples
    Catch-and-Release: Extraction and Purification of NGS-Grade DNA...
    Formalin Fixed, Paraffin Embedded (FFPE) tissues are rapidly being adopted for transcriptome analysis in conjunction with targeted and whole genome sequencing. Harsh formaldehyde fixation an...
    Speaker: Guillaume Durin, PhD
    Sponsored By: Covaris, Inc., Covaris, Inc.
    10
    JAN 21, 2015 | 10:00 PM
    NGS-Quality Sample Preparation with Covaris Adaptive Focused Acoustics (AFA™)
    NGS-Quality Sample Preparation with Covaris Adaptive Focused Acoustics...
     In this presentation we will illustrate the advantages of AFA technology in clinically significant applications (NGS, FFPE, ChIP) and will demonstrate the flexibility of Covaris AFA in deliv...
    Speaker: Hamid Khoja Ph.D.
    Sponsored By: Covaris, Inc., Covaris, Inc.
    8
     
     
  • AUG 21, 2014 | 11:30 AM
    Automation of High Throughput Agarose Gel Electrophoresis with the NIMBUS Select Featuring Ranger Technology
    Automation of High Throughput Agarose Gel Electrophoresis with...
    Coastal Genomics and the Hamilton Company have teamed up to combine the NIMBUS 96 channel platform with Ranger Technology to automate high throughput agarose gel electrophoresis. The new NIM...
    Speaker: Matthew Nesbitt, MSc
    Presented at: Genetics and Genomics Virtual Event Series 2014
    0
    AUG 20, 2014 | 7:30 AM
    Panel Discussion: Increasing productivity and reproducibility by automating DNA library preparation for Ion Torrent Sequencing
    Panel Discussion: Increasing productivity and reproducibility...
    Next Generation Sequencing has revolutionized genomic variant discovery. The major bottle-neck for sequencing projects is the individual, biological sample, library preparation. This platfo...
    Speaker: Joanna Hamilton, PhD, Lynne Apone, PhD, Zach Smith, MS
    Presented at: Genetics and Genomics Virtual Event Series 2014
    12
    OCT 17, 2013 | 7:00 AM
    Advancing Translational Cancer Research through Automated RNASeq Sample Preparation from Archival FFPE Tissue Specimens
    Advancing Translational Cancer Research through Automated RNASeq...
    RNA-Seq allows the simultaneous observation of gene expression levels, mutations in the coding sequences, splice variants and gene fusions, which are especially important in cancer studies. A...
    Speaker: Michaela Bowden, PhD
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2013
    98
    AUG 21, 2013 | 11:00 AM
    Panel Discussion: Meeting the Demand of Increased NGS Capacity with Automated TruSeq Stranded mRNA Library Preparation
    Panel Discussion: Meeting the Demand of Increased NGS Capacity...
    RNASeq has become the primary method for studying changes in gene expression in modern genomics research. By directly sequencing the various types of RNA molecules, including mRNA, ncRNA, an...
    Speaker: Alisa Jackson, Zach Smith, Mary Blair, Dave Delano
    Presented at: Genetics and Genomics Virtual Event Series 2013
    41
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