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    Microvesicles

    Microvesicles are a type of extracellular vesicle, between 50 and 1,000 nanometers in diameter, found in many types of body fluids as well as the interstitial space between cells. Microvesicles are membrane-bound vesicles containing phospholipids, ranging from 100 nm to 1000 nm shed from almost all cell types.

    Webinars

    • JUL 19, 2022 | 8:00 AM
      Getting started in Cryo-EM
      Getting started in Cryo-EM
      Date: July 19, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Cryo-EM is rapidly becoming the method of choice for structural biologists and biochemists around the world, helping thes...
      Speaker: Melissa Chambers , Natalia de Val
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      9
      NOV 17, 2021 | 8:00 AM
      Exosomes: From pure isolation to standardized analysis by flow cytometry
      Exosomes: From pure isolation to standardized analysis by flow...
      Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
      Speaker: Ariadna Pascual Velazquez , Laura Müller , Stefan Wild, PhD
      Sponsored By: Miltenyi Biotec
      9
      SEP 14, 2021 | 7:00 AM
      What prevents you from increasing your PCR productivity?
      What prevents you from increasing your PCR productivity?
      Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
      Speaker: Arora Phang, PhD
      Sponsored By: Eppendorf
      24 1
      JUL 27, 2021 | 8:00 AM
      Scaling-Up Exosomes and Other Extracellular Vesicles
      Scaling-Up Exosomes and Other Extracellular Vesicles
      Date: July 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) The Origin of Biopharmaceutics (Biopharma) derive from The Division of Clinical Research in the U.S. Bureau of Medicine. However, author...
      Speaker: Dr. Derek Lenz
      Sponsored By: Beckman Coulter Life Sciences
      16 3
      FEB 24, 2021 | 10:00 AM
      Biosafety Enclosures and the Automated Lab: Steps for Success
      Biosafety Enclosures and the Automated Lab: Steps for Success
      DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
      Speaker: David Wasescha , Simon Fogarty
      Sponsored By: Labconco
      15
      SEP 23, 2020 | 12:00 AM
      Current approaches for exosome processing and the emerging importance of subtyping
      Current approaches for exosome processing and the emerging importance...
      Learning Objectives: 1. Understand methods of isolating and enriching exosomes along with their pros/cons of the different techniques 2. What do we mean when we talk about subtyping, and wha...
      Speaker: Jim West III, MS, CEO
      Presented at: Cell Biology Virtual Event Series 2020
      1
      SEP 03, 2020 | 9:00 AM
      The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
      The Complexities of SV Analytical Ultracentrifugation: AAVs are...
      DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
      Speaker: Dr. Lake Paul
      Sponsored By: Beckman Coulter Life Sciences
      12
      APR 30, 2020 | 8:00 AM
      Uncovering the membrane mechanism of cytokinesis using live cell imaging and subcellular optogenetic tools.
      Uncovering the membrane mechanism of cytokinesis using live cell...
      DATE:April 30, 2020 TIME: 8AM PT, 11AM ET, 4PM BST, 5PM CEST Cytokinesis is the physical separation of two cells that occurs after the completion of mitosis. The mechanism underlying it is v...
      Speaker: Alan Mullan, PhD , Jean Castillo-Badillo, PhD , Claudia Florindo, PhD
      Sponsored By: Andor
      7 2
      SEP 13, 2018 | 1:30 PM
      New PacBio Solutions for High-Resolution Microbiology & Immunology Analysis
      New PacBio Solutions for High-Resolution Microbiology & Immunology...
      PacBio Sequencing has been recognized as the gold standard in microbial sequencing due to simultaneously providing long sequence reads (genome contiguity), high consensus accuracy (genome acc...
      Speaker: Jonas Korlach, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      Sponsored By: PacBio
      1
      SEP 13, 2018 | 1:30 PM
      Mechanisms of Antimicrobial Bioconversion by Environmental and Host-Associated Bacteria
      Mechanisms of Antimicrobial Bioconversion by Environmental and...
      The soil microbiome can produce, resist, or degrade antibiotics and even catabolize them. Resistance genes are widely distributed in the soil and may act as a reservoir for pathogen antibioti...
      Speaker: Terence Crofts, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      3
      SEP 13, 2018 | 12:00 PM
      Symbiotic Programing of Plants for Stress Tolerance with Fungal Endophytes: Altering the Future of Food Security, Political Stability and Poverty
      Symbiotic Programing of Plants for Stress Tolerance with Fungal...
      The greatest need in agriculture this century is to mitigate impacts of abiotic (drought, temperature, salinity) and biotic (pathogens, pests) stresses on crop plants.  Efforts over the...
      Speaker: Rusty Rodriguez, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      3
      SEP 13, 2018 | 12:00 PM
      Disrupting Sexually Transmitted Diseases by Leveraging Innovative Technologies for Prevention: An Overview of Global and Local STD Trends and Impact
      Disrupting Sexually Transmitted Diseases by Leveraging Innovative...
      Sexually transmitted diseases remain a major global public health burden despite the availability of effective prevention strategies to diagnose and treat bacterial STDs.  Concerning tre...
      Speaker: Joan Chow, MPH, DrPH
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      2
      SEP 13, 2018 | 12:00 PM
      Outer Membrane Remodeling of Attenuated Salmonella to Create a Mucosal Vaccine Against Acinetobacter Baumannii
      Outer Membrane Remodeling of Attenuated Salmonella to Create a...
      While rapid identification of pathogens, novel therapeutic interventions, and passive immunization have critical roles in disease control, none can substitute for pre-existing protective immu...
      Speaker: James Galen, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      SEP 13, 2018 | 10:30 AM
      Studying Environmental Sensing and Response in a Bacterial Plant Pathogen - Inside Out
      Studying Environmental Sensing and Response in a Bacterial Plant...
      The bacterium Pantoea stewartii subsp. stewartii causes Stewart’s wilt disease in corn and serves as a model for other xylem-dwelling phytopathogens. P. stewartii is transmitted to plan...
      Speaker: Ann Stevens, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      3
      SEP 13, 2018 | 10:30 AM
      Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic Resistance
      Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic...
      Horizontal gene transfer (HGT) – or the non-genealogical transmission of DNA between organisms – is the dominant mode responsible for the spread of antibiotic resistance genes. Co...
      Speaker: Allison Lopatkin, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      3
      SEP 13, 2018 | 9:00 AM
      Novel Solutions for Unbiased Results in Metagenomic Analysis of Human Microbiome Samples
      Novel Solutions for Unbiased Results in Metagenomic Analysis of...
      This two-part webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples. Part 1 will feature QIAGEN’s solutions...
      Speaker: Matthew Fosbrink, PhD , Dominic O'Neil
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      SEP 13, 2018 | 9:00 AM
      Viruses in Soils - What Do We Know, and Where Do We Go From Here?
      Viruses in Soils - What Do We Know, and Where Do We Go From Here?
      Viruses outnumber bacteria in soil by as much as 1000:1. However, there are few studies which examine the ways viral communities impact the soil microbiome, chemical cycles, and plants. Recen...
      Speaker: Laura Zinke, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      2
      SEP 13, 2018 | 7:30 AM
      Keynote Presentation: Tracking the Resistome in One Health Surveillance
      Keynote Presentation: Tracking the Resistome in One Health Surveillance
      In the US, the National Antimicrobial Resistance Monitoring System has been using WGS of Salmonella as a tool of routine surveillance since 2013. To date, NARMS has generated MIC and WGS data...
      Speaker: Patrick McDermott, MS, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      2
      SEP 13, 2018 | 6:00 AM
      The Evolution of Diverse, Ubiquitous CRESS DNA Viruses
      The Evolution of Diverse, Ubiquitous CRESS DNA Viruses
      In the US, the National Antimicrobial Resistance Monitoring System has been using WGS of Salmonella as a tool of routine surveillance since 2013. To date, NARMS has generated MIC and WGS data...
      Speaker: Siobain Duffy, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      SEP 13, 2018 | 6:00 AM
      The Hurricane Microbiome Project: Environmental Exposures and Population Health
      The Hurricane Microbiome Project: Environmental Exposures and...
      The Alkek Center for Metagenomics and Microbiome Research (CMMR) at Baylor College of Medicine is pursuing numerous research and development efforts in the study of how the microbiome impacts...
      Speaker: Joseph Petrosino, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      SEP 12, 2018 | 1:30 PM
      Connect Across 1000s of Expertly Curated Genomics Datasets Using QIAGEN's Immunology Focused DiseaseLand Database
      Connect Across 1000s of Expertly Curated Genomics Datasets Using...
      DiseaseLand is an integrated genomics database and visualization software that helps researchers explore 1000s of public and private datasets covering, but not limited to therapeutic areas su...
      Speaker: Lucy Williams, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      SEP 12, 2018 | 1:30 PM
      The NCTC 3000 Project: Mapping the Genetic Codes of 3000 Pathogenic Bacteria
      The NCTC 3000 Project: Mapping the Genetic Codes of 3000 Pathogenic...
      Reducing the impact of infectious diseases is becoming increasingly vital as the prevalence of antimicrobial resistance (AMR) continues to rise, threatening the effective prevention and treat...
      Speaker: Julie Russell, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      SEP 12, 2018 | 12:00 PM
      Antimicrobial Resistance Identification, Strain Typing, and Assay Design with CLC Genomics Workbench
      Antimicrobial Resistance Identification, Strain Typing, and Assay...
      Whole genome sequencing of bacterial isolates via next-generation sequencing is becoming a standard approach for clinical microbiology and bio-surveillance labs seeking to conduct outbreak in...
      Speaker: Winnie Ridderberg, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      Sponsored By: QIAGEN
      3
      SEP 12, 2018 | 12:00 PM
      ONETest PathoGenome: A Target Hybridization-Based Next-Generation Sequencing Assay to Tackle Undiagnosed Infections
      ONETest PathoGenome: A Target Hybridization-Based Next-Generation...
      Every year, approximately 800,000 critically ill patients are hospitals in the USA with undiagnosed infections. The only option is to treat these patients with broad-spectrum antimicrobial th...
      Speaker: Mohammad Qadir, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1 1
    JUL 19, 2022 | 8:00 AM
    Getting started in Cryo-EM
    Getting started in Cryo-EM
    Date: July 19, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Cryo-EM is rapidly becoming the method of choice for structural biologists and biochemists around the world, helping thes...
    Speaker: Melissa Chambers , Natalia de Val
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    9
    NOV 17, 2021 | 8:00 AM
    Exosomes: From pure isolation to standardized analysis by flow cytometry
    Exosomes: From pure isolation to standardized analysis by flow...
    Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
    Speaker: Ariadna Pascual Velazquez , Laura Müller , Stefan Wild, PhD
    Sponsored By: Miltenyi Biotec
    9
    SEP 14, 2021 | 7:00 AM
    What prevents you from increasing your PCR productivity?
    What prevents you from increasing your PCR productivity?
    Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
    Speaker: Arora Phang, PhD
    Sponsored By: Eppendorf
    24 1
    JUL 27, 2021 | 8:00 AM
    Scaling-Up Exosomes and Other Extracellular Vesicles
    Scaling-Up Exosomes and Other Extracellular Vesicles
    Date: July 27, 2021 Time: 8:00am (PDT), 11:00am (EDT) The Origin of Biopharmaceutics (Biopharma) derive from The Division of Clinical Research in the U.S. Bureau of Medicine. However, author...
    Speaker: Dr. Derek Lenz
    Sponsored By: Beckman Coulter Life Sciences
    16 3
    FEB 24, 2021 | 10:00 AM
    Biosafety Enclosures and the Automated Lab: Steps for Success
    Biosafety Enclosures and the Automated Lab: Steps for Success
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    Speaker: David Wasescha , Simon Fogarty
    Sponsored By: Labconco
    15
     
     
  • SEP 23, 2020 | 12:00 AM
    Current approaches for exosome processing and the emerging importance of subtyping
    Current approaches for exosome processing and the emerging importance...
    Learning Objectives: 1. Understand methods of isolating and enriching exosomes along with their pros/cons of the different techniques 2. What do we mean when we talk about subtyping, and wha...
    Speaker: Jim West III, MS, CEO
    Presented at: Cell Biology Virtual Event Series 2020
    1
    SEP 03, 2020 | 9:00 AM
    The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
    The Complexities of SV Analytical Ultracentrifugation: AAVs are...
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    Speaker: Dr. Lake Paul
    Sponsored By: Beckman Coulter Life Sciences
    12
    APR 30, 2020 | 8:00 AM
    Uncovering the membrane mechanism of cytokinesis using live cell imaging and subcellular optogenetic tools.
    Uncovering the membrane mechanism of cytokinesis using live cell...
    DATE:April 30, 2020 TIME: 8AM PT, 11AM ET, 4PM BST, 5PM CEST Cytokinesis is the physical separation of two cells that occurs after the completion of mitosis. The mechanism underlying it is v...
    Speaker: Alan Mullan, PhD , Jean Castillo-Badillo, PhD , Claudia Florindo, PhD
    Sponsored By: Andor
    7 2
    SEP 13, 2018 | 1:30 PM
    New PacBio Solutions for High-Resolution Microbiology & Immunology Analysis
    New PacBio Solutions for High-Resolution Microbiology & Immunology...
    PacBio Sequencing has been recognized as the gold standard in microbial sequencing due to simultaneously providing long sequence reads (genome contiguity), high consensus accuracy (genome acc...
    Speaker: Jonas Korlach, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    Sponsored By: PacBio
    1
    SEP 13, 2018 | 1:30 PM
    Mechanisms of Antimicrobial Bioconversion by Environmental and Host-Associated Bacteria
    Mechanisms of Antimicrobial Bioconversion by Environmental and...
    The soil microbiome can produce, resist, or degrade antibiotics and even catabolize them. Resistance genes are widely distributed in the soil and may act as a reservoir for pathogen antibioti...
    Speaker: Terence Crofts, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    3
     
     
  • SEP 13, 2018 | 12:00 PM
    Symbiotic Programing of Plants for Stress Tolerance with Fungal Endophytes: Altering the Future of Food Security, Political Stability and Poverty
    Symbiotic Programing of Plants for Stress Tolerance with Fungal...
    The greatest need in agriculture this century is to mitigate impacts of abiotic (drought, temperature, salinity) and biotic (pathogens, pests) stresses on crop plants.  Efforts over the...
    Speaker: Rusty Rodriguez, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    3
    SEP 13, 2018 | 12:00 PM
    Disrupting Sexually Transmitted Diseases by Leveraging Innovative Technologies for Prevention: An Overview of Global and Local STD Trends and Impact
    Disrupting Sexually Transmitted Diseases by Leveraging Innovative...
    Sexually transmitted diseases remain a major global public health burden despite the availability of effective prevention strategies to diagnose and treat bacterial STDs.  Concerning tre...
    Speaker: Joan Chow, MPH, DrPH
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    2
    SEP 13, 2018 | 12:00 PM
    Outer Membrane Remodeling of Attenuated Salmonella to Create a Mucosal Vaccine Against Acinetobacter Baumannii
    Outer Membrane Remodeling of Attenuated Salmonella to Create a...
    While rapid identification of pathogens, novel therapeutic interventions, and passive immunization have critical roles in disease control, none can substitute for pre-existing protective immu...
    Speaker: James Galen, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
    SEP 13, 2018 | 10:30 AM
    Studying Environmental Sensing and Response in a Bacterial Plant Pathogen - Inside Out
    Studying Environmental Sensing and Response in a Bacterial Plant...
    The bacterium Pantoea stewartii subsp. stewartii causes Stewart’s wilt disease in corn and serves as a model for other xylem-dwelling phytopathogens. P. stewartii is transmitted to plan...
    Speaker: Ann Stevens, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    3
    SEP 13, 2018 | 10:30 AM
    Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic Resistance
    Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic...
    Horizontal gene transfer (HGT) – or the non-genealogical transmission of DNA between organisms – is the dominant mode responsible for the spread of antibiotic resistance genes. Co...
    Speaker: Allison Lopatkin, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    3
     
     
  • SEP 13, 2018 | 9:00 AM
    Novel Solutions for Unbiased Results in Metagenomic Analysis of Human Microbiome Samples
    Novel Solutions for Unbiased Results in Metagenomic Analysis of...
    This two-part webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples. Part 1 will feature QIAGEN’s solutions...
    Speaker: Matthew Fosbrink, PhD , Dominic O'Neil
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
    SEP 13, 2018 | 9:00 AM
    Viruses in Soils - What Do We Know, and Where Do We Go From Here?
    Viruses in Soils - What Do We Know, and Where Do We Go From Here?
    Viruses outnumber bacteria in soil by as much as 1000:1. However, there are few studies which examine the ways viral communities impact the soil microbiome, chemical cycles, and plants. Recen...
    Speaker: Laura Zinke, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    2
    SEP 13, 2018 | 7:30 AM
    Keynote Presentation: Tracking the Resistome in One Health Surveillance
    Keynote Presentation: Tracking the Resistome in One Health Surveillance
    In the US, the National Antimicrobial Resistance Monitoring System has been using WGS of Salmonella as a tool of routine surveillance since 2013. To date, NARMS has generated MIC and WGS data...
    Speaker: Patrick McDermott, MS, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    2
    SEP 13, 2018 | 6:00 AM
    The Evolution of Diverse, Ubiquitous CRESS DNA Viruses
    The Evolution of Diverse, Ubiquitous CRESS DNA Viruses
    In the US, the National Antimicrobial Resistance Monitoring System has been using WGS of Salmonella as a tool of routine surveillance since 2013. To date, NARMS has generated MIC and WGS data...
    Speaker: Siobain Duffy, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
    SEP 13, 2018 | 6:00 AM
    The Hurricane Microbiome Project: Environmental Exposures and Population Health
    The Hurricane Microbiome Project: Environmental Exposures and...
    The Alkek Center for Metagenomics and Microbiome Research (CMMR) at Baylor College of Medicine is pursuing numerous research and development efforts in the study of how the microbiome impacts...
    Speaker: Joseph Petrosino, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
     
     
  • SEP 12, 2018 | 1:30 PM
    Connect Across 1000s of Expertly Curated Genomics Datasets Using QIAGEN's Immunology Focused DiseaseLand Database
    Connect Across 1000s of Expertly Curated Genomics Datasets Using...
    DiseaseLand is an integrated genomics database and visualization software that helps researchers explore 1000s of public and private datasets covering, but not limited to therapeutic areas su...
    Speaker: Lucy Williams, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
    SEP 12, 2018 | 1:30 PM
    The NCTC 3000 Project: Mapping the Genetic Codes of 3000 Pathogenic Bacteria
    The NCTC 3000 Project: Mapping the Genetic Codes of 3000 Pathogenic...
    Reducing the impact of infectious diseases is becoming increasingly vital as the prevalence of antimicrobial resistance (AMR) continues to rise, threatening the effective prevention and treat...
    Speaker: Julie Russell, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
    SEP 12, 2018 | 12:00 PM
    Antimicrobial Resistance Identification, Strain Typing, and Assay Design with CLC Genomics Workbench
    Antimicrobial Resistance Identification, Strain Typing, and Assay...
    Whole genome sequencing of bacterial isolates via next-generation sequencing is becoming a standard approach for clinical microbiology and bio-surveillance labs seeking to conduct outbreak in...
    Speaker: Winnie Ridderberg, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    Sponsored By: QIAGEN
    3
    SEP 12, 2018 | 12:00 PM
    ONETest PathoGenome: A Target Hybridization-Based Next-Generation Sequencing Assay to Tackle Undiagnosed Infections
    ONETest PathoGenome: A Target Hybridization-Based Next-Generation...
    Every year, approximately 800,000 critically ill patients are hospitals in the USA with undiagnosed infections. The only option is to treat these patients with broad-spectrum antimicrobial th...
    Speaker: Mohammad Qadir, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1 1
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