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    Monolayers

    A monolayer is a single layer of atoms, molecules, or cells where each individual component is tightly packed to the next. The thickness of a monolayer equals the width of a single atom, molecule, or cell.

    Webinars

    • NOV 08, 2023 | 9:00 AM
      Epithelium Formation: Cellular Adhesion Regulation in 3D
      Epithelium Formation: Cellular Adhesion Regulation in 3D
      Most internal organs consist of a monolayer of polarized epithelial cells surrounding a central lumen that establish a barrier that segregates the internal medium from the outside environmen...
      Speaker: Rafael Garcia-Mata, Ph.D.
      Sponsored By: Leica Microsystems
      13
      APR 26, 2022 | 7:00 AM
      Modelling of cellular interactions in mixed murine organoids
      Modelling of cellular interactions in mixed murine organoids
      Date: April 26, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CET) Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We...
      Speaker: Saskia J.E Suijkerbuijk
      Sponsored By: Andor
      8
      SEP 22, 2021 | 11:59 PM
      Mechanisms of Podocyte Damage in Alport Syndrome: Insights from the Alport-on-a-chip System
      Mechanisms of Podocyte Damage in Alport Syndrome: Insights from...
      Microtubule plus-end tracking proteins (+TIPs) play key roles in the regulation of microtubule dynamics in vivo. These proteins bind to and regulate microtubule “plus‐ends”, whic...
      Speaker: Astgik Petrosyan, PhD
      Presented at: Cell Biology Virtual Event Series 2021
      0
      APR 20, 2021 | 10:00 AM
      Use of a New 3D Pluripotent Stem Cell Suspension Culture Medium for Rapid Scale-Up and Downstream Differentiation of Pluripotent Stem Cells
      Use of a New 3D Pluripotent Stem Cell Suspension Culture Medium...
      Date: April 20, 2021 Time: 10:00am (PST) In order to generate sufficient numbers of pluripotent stem cells (PSCs) for downstream applications, three-dimensional (3D) suspension cultures offe...
      Speaker: Michael Akenhead , Michael Derr
      Sponsored By: Thermo Fisher Scientific
      5
      FEB 03, 2021 | 12:30 PM
      Optimizing High-Content Imaging of 3D Models for Drug Discovery
      Optimizing High-Content Imaging of 3D Models for Drug Discovery
      In vitro spheroid models are fast becoming the de facto standard for drug discovery applications, largely due to their human-like physiological and morphological characteristics, tissue-like...
      Speaker: Arvonn Tully , Judi Wardwell-Swanson
      Presented at: 7th Annual Conference: SBI2 High Content
      Sponsored By: SBI2, InSphero , Yokogawa
      11 10
      November 13, 2020 4:00 PM PST | November 14, 2020 8:00 AM SGT
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
      Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
      Speaker: Srabani Bhaumik, PhD
      Presented at: DISCOVERY Global User Symposium
      1
      November 12, 2020 9:00 AM PST | 4:00 PM GMT
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
      Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
      Speaker: Srabani Bhaumik, PhD
      Presented at: DISCOVERY Global User Symposium
      1
      SEP 17, 2020 | 2:00 PM
      MAPK Activity Dynamics Regulate Non-Cell Autonomous Effects of Oncogene Expression
      MAPK Activity Dynamics Regulate Non-Cell Autonomous Effects of...
      Deregulation of Receptor Tyrosine Kinase (RTK) signaling underlies a large fraction of human cancers. These genetic perturbations lead to a variety of cell autonomous and non-cell autonomous...
      Speaker: Sergi Regot, MS, PhD
      Presented at: 7th Annual Conference: SBI2 High Content
      2
      JUN 03, 2020 | 12:00 AM
      ALAn: A Tool for Automated Analysis of Epithelial Layer Architecture
      ALAn: A Tool for Automated Analysis of Epithelial Layer Architecture
      Madin Darby Canine Kidney (MDCK) cells can form a polarized epithelial layer in culture. We and others have observed that these layers are frequently characterized by extralayer cells, posit...
      Speaker: Nicole Dawney
      Presented at: Cell & Developmental Biology Virtual Meeting
      1
      SEP 24, 2019 | 8:00 AM
      Interrogating 3D spheroid versus 2D monolayer cell models in drug discovery using cell health microplate assays
      Interrogating 3D spheroid versus 2D monolayer cell models in drug...
      DATE: September 24, 2019TIME: 7:00am PDTHigh throughput screening (HTS) is an effective method for identifying putative active compounds for therapeutics. Assays that evaluate changes i...
      Speaker: Dr. Adyary Fallarero, Ph.D. Adjunct Professor/Docent in Pharmaceutical Biology , Dr. Leticia Montoya, Ph.D., Chemistry and Biochemistry
      Sponsored By: Thermo Fisher Scientific
      9
      JUN 27, 2019 | 9:00 AM
      CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system for studying cellular microenvironment
      CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system...
      DATE: June 27, 2019TIME: 9:00am PDT, 12:00pm EDT At present cancer research focuses on three major areas viz. cancer diagnostics, drugs development, and next-generation therapi...
      Speaker: Vivek Kamat, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      12
      OCT 01, 2018 | 12:00 AM
      Efficient generation of pancreatic β cell precursors from human pluripotent stem cells
      Efficient generation of pancreatic β cell precursors from human...
      Pancreatic β cell replacement therapy is considered as a potential strategy to treat diabetes. To date, transplantation of pancreatic islets from cadavers is the most effective approach...
      Speaker: Essam Abdelalim
      Presented at: 6th annual 24 Hours of Stem Cells™ virtual event
      4
      SEP 26, 2018 | 1:30 PM
      Two-Dimensional and Three-Dimensional Cell Culture, Does Dimension Really Matter?
      Two-Dimensional and Three-Dimensional Cell Culture, Does Dimension...
      When looking to answer complex biological questions, using in vitro techniques, you want the model that best reflects the in vivo condition.  For decades two-dimensional cell culture has...
      Speaker: Carlemi Calitz, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      2
      SEP 26, 2018 | 12:00 PM
      3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
      3D Cell Cultures and Tissue Clearing: A High Content Confocal...
      With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
      Speaker: Michael Johnson, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      2
      AUG 09, 2018 | 7:00 AM
      3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
      3D Cell Cultures and Tissue Clearing: A High Content Confocal...
      With the advent of cost effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
      Speaker: Michael Johnson, PhD
      Sponsored By: Thermo Fisher Scientific
      7
      AUG 01, 2018 | 12:00 AM
      Autologous iPS cell therapy for Macular Degeneration: From bench-to-bedside
      Autologous iPS cell therapy for Macular Degeneration: From bench-to-bedside
      Induced pluripotent stem (iPS) cells are a promising source of personalized therapy. These cells can provide immune-compatible autologous replacement tissue for the treatment of potentially a...
      Speaker: Kapil Bharti
      Presented at: 6th annual 24 Hours of Stem Cells™ virtual event
      3
      NOV 16, 2017 | 9:00 AM
      WEBINAR: Breaking Bad with 3D: The development of high throughput screening platforms to interrogate physiologically relevant models
      WEBINAR: Breaking Bad with 3D: The development of high throughput...
      DATE: November 16, 2017TIME: 9:00AM PT, 12:00PM ETThe wide use of 2D monolayer cultures for cancer drug discovery reflects the technical ease of implementation for drug screening, and t...
      Speaker: Madhu Nag, PhD
      Sponsored By: Revvity
      4
      JUL 01, 2017 | 8:00 AM
      Integrated processes for derivation, expansion and differentiation of hiPSC to blood in IPS-Spheres™ cultures
      Integrated processes for derivation, expansion and differentiation...
      Current methods for human induced pluripotent stem cells (hiPSC) derivation, expansion and differentiation can be limited in scalability and costly (due to their labor intensive nature). This...
      Speaker: Alan Lam
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      1
      JUN 07, 2017 | 9:00 AM
      WEBINAR: Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflow
      WEBINAR: Generation of purified human iPSC derived cardiomyocytes...
      DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
      Speaker: Todd Herron, BS, PhD
      Sponsored By: Miltenyi Biotec
      5 1
      FEB 23, 2017 | 12:00 PM
      Recent progress in cellular medicine with an eye to precision medicine
      Recent progress in cellular medicine with an eye to precision...
      Despite vast investment for decades in cancer research and development, cancer is still among the leading causes of death worldwide and its toll is expected to rise by about 70% over the next...
      Speaker: Giacinto Scoles, PhD
      Presented at: Precision Medicine Virtual Event Series 2017
      2
      OCT 18, 2016 | 1:00 PM
      The Role of 3D Culture Methods in Retaining Native Biological Properties of Translational In Vitro Models
      The Role of 3D Culture Methods in Retaining Native Biological...
      Cell Sciences Innovation, Merck Research Laboratories, 2015 Galloping Hill Road, Kenilworth, NJ USA In vitro translational models play a critical role in the drug discovery process because t...
      Speaker: Richard Visconti, PhD
      Presented at: SyncD³ - Where drug discovery & development meet
      0
      OCT 05, 2016 | 7:30 AM
      Keynote Speaker: How to meet the challenge of counting and analyzing tumor cells circulating in the peripheral blood
      Keynote Speaker: How to meet the challenge of counting and analyzing...
      The talk will first report on a new patented method of counting CTCs based on the metabolism of tumor cells, that favors acidification of their near environment. In order to maintain the acid...
      Speaker: Giacinto Scoles, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      3
      SEP 20, 2016 | 8:00 AM
      Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions
      Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions
      DATE: September 20, 2016 TIME: 8:00am PST, 11:00 EST Studies of cell-substrate interactions are important for an array of biotechnology applications.  The nature of specific, recepto...
      Speaker: Michael Hill
      Sponsored By: Ametek, Ametek , Ametek
      7 5
    NOV 08, 2023 | 9:00 AM
    Epithelium Formation: Cellular Adhesion Regulation in 3D
    Epithelium Formation: Cellular Adhesion Regulation in 3D
    Most internal organs consist of a monolayer of polarized epithelial cells surrounding a central lumen that establish a barrier that segregates the internal medium from the outside environmen...
    Speaker: Rafael Garcia-Mata, Ph.D.
    Sponsored By: Leica Microsystems
    13
    APR 26, 2022 | 7:00 AM
    Modelling of cellular interactions in mixed murine organoids
    Modelling of cellular interactions in mixed murine organoids
    Date: April 26, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CET) Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We...
    Speaker: Saskia J.E Suijkerbuijk
    Sponsored By: Andor
    8
    SEP 22, 2021 | 11:59 PM
    Mechanisms of Podocyte Damage in Alport Syndrome: Insights from the Alport-on-a-chip System
    Mechanisms of Podocyte Damage in Alport Syndrome: Insights from...
    Microtubule plus-end tracking proteins (+TIPs) play key roles in the regulation of microtubule dynamics in vivo. These proteins bind to and regulate microtubule “plus‐ends”, whic...
    Speaker: Astgik Petrosyan, PhD
    Presented at: Cell Biology Virtual Event Series 2021
    0
    APR 20, 2021 | 10:00 AM
    Use of a New 3D Pluripotent Stem Cell Suspension Culture Medium for Rapid Scale-Up and Downstream Differentiation of Pluripotent Stem Cells
    Use of a New 3D Pluripotent Stem Cell Suspension Culture Medium...
    Date: April 20, 2021 Time: 10:00am (PST) In order to generate sufficient numbers of pluripotent stem cells (PSCs) for downstream applications, three-dimensional (3D) suspension cultures offe...
    Speaker: Michael Akenhead , Michael Derr
    Sponsored By: Thermo Fisher Scientific
    5
    FEB 03, 2021 | 12:30 PM
    Optimizing High-Content Imaging of 3D Models for Drug Discovery
    Optimizing High-Content Imaging of 3D Models for Drug Discovery
    In vitro spheroid models are fast becoming the de facto standard for drug discovery applications, largely due to their human-like physiological and morphological characteristics, tissue-like...
    Speaker: Arvonn Tully , Judi Wardwell-Swanson
    Presented at: 7th Annual Conference: SBI2 High Content
    Sponsored By: SBI2, InSphero , Yokogawa
    11 10
     
     
  • November 13, 2020 4:00 PM PST | November 14, 2020 8:00 AM SGT
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
    Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
    Speaker: Srabani Bhaumik, PhD
    Presented at: DISCOVERY Global User Symposium
    1
    November 12, 2020 9:00 AM PST | 4:00 PM GMT
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
    Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
    Speaker: Srabani Bhaumik, PhD
    Presented at: DISCOVERY Global User Symposium
    1
    SEP 17, 2020 | 2:00 PM
    MAPK Activity Dynamics Regulate Non-Cell Autonomous Effects of Oncogene Expression
    MAPK Activity Dynamics Regulate Non-Cell Autonomous Effects of...
    Deregulation of Receptor Tyrosine Kinase (RTK) signaling underlies a large fraction of human cancers. These genetic perturbations lead to a variety of cell autonomous and non-cell autonomous...
    Speaker: Sergi Regot, MS, PhD
    Presented at: 7th Annual Conference: SBI2 High Content
    2
    JUN 03, 2020 | 12:00 AM
    ALAn: A Tool for Automated Analysis of Epithelial Layer Architecture
    ALAn: A Tool for Automated Analysis of Epithelial Layer Architecture
    Madin Darby Canine Kidney (MDCK) cells can form a polarized epithelial layer in culture. We and others have observed that these layers are frequently characterized by extralayer cells, posit...
    Speaker: Nicole Dawney
    Presented at: Cell & Developmental Biology Virtual Meeting
    1
    SEP 24, 2019 | 8:00 AM
    Interrogating 3D spheroid versus 2D monolayer cell models in drug discovery using cell health microplate assays
    Interrogating 3D spheroid versus 2D monolayer cell models in drug...
    DATE: September 24, 2019TIME: 7:00am PDTHigh throughput screening (HTS) is an effective method for identifying putative active compounds for therapeutics. Assays that evaluate changes i...
    Speaker: Dr. Adyary Fallarero, Ph.D. Adjunct Professor/Docent in Pharmaceutical Biology , Dr. Leticia Montoya, Ph.D., Chemistry and Biochemistry
    Sponsored By: Thermo Fisher Scientific
    9
     
     
  • JUN 27, 2019 | 9:00 AM
    CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system for studying cellular microenvironment
    CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system...
    DATE: June 27, 2019TIME: 9:00am PDT, 12:00pm EDT At present cancer research focuses on three major areas viz. cancer diagnostics, drugs development, and next-generation therapi...
    Speaker: Vivek Kamat, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    12
    OCT 01, 2018 | 12:00 AM
    Efficient generation of pancreatic β cell precursors from human pluripotent stem cells
    Efficient generation of pancreatic β cell precursors from human...
    Pancreatic β cell replacement therapy is considered as a potential strategy to treat diabetes. To date, transplantation of pancreatic islets from cadavers is the most effective approach...
    Speaker: Essam Abdelalim
    Presented at: 6th annual 24 Hours of Stem Cells™ virtual event
    4
    SEP 26, 2018 | 1:30 PM
    Two-Dimensional and Three-Dimensional Cell Culture, Does Dimension Really Matter?
    Two-Dimensional and Three-Dimensional Cell Culture, Does Dimension...
    When looking to answer complex biological questions, using in vitro techniques, you want the model that best reflects the in vivo condition.  For decades two-dimensional cell culture has...
    Speaker: Carlemi Calitz, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    2
    SEP 26, 2018 | 12:00 PM
    3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
    3D Cell Cultures and Tissue Clearing: A High Content Confocal...
    With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
    Speaker: Michael Johnson, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    2
    AUG 09, 2018 | 7:00 AM
    3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
    3D Cell Cultures and Tissue Clearing: A High Content Confocal...
    With the advent of cost effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
    Speaker: Michael Johnson, PhD
    Sponsored By: Thermo Fisher Scientific
    7
     
     
  • AUG 01, 2018 | 12:00 AM
    Autologous iPS cell therapy for Macular Degeneration: From bench-to-bedside
    Autologous iPS cell therapy for Macular Degeneration: From bench-to-bedside
    Induced pluripotent stem (iPS) cells are a promising source of personalized therapy. These cells can provide immune-compatible autologous replacement tissue for the treatment of potentially a...
    Speaker: Kapil Bharti
    Presented at: 6th annual 24 Hours of Stem Cells™ virtual event
    3
    NOV 16, 2017 | 9:00 AM
    WEBINAR: Breaking Bad with 3D: The development of high throughput screening platforms to interrogate physiologically relevant models
    WEBINAR: Breaking Bad with 3D: The development of high throughput...
    DATE: November 16, 2017TIME: 9:00AM PT, 12:00PM ETThe wide use of 2D monolayer cultures for cancer drug discovery reflects the technical ease of implementation for drug screening, and t...
    Speaker: Madhu Nag, PhD
    Sponsored By: Revvity
    4
    JUL 01, 2017 | 8:00 AM
    Integrated processes for derivation, expansion and differentiation of hiPSC to blood in IPS-Spheres™ cultures
    Integrated processes for derivation, expansion and differentiation...
    Current methods for human induced pluripotent stem cells (hiPSC) derivation, expansion and differentiation can be limited in scalability and costly (due to their labor intensive nature). This...
    Speaker: Alan Lam
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    1
    JUN 07, 2017 | 9:00 AM
    WEBINAR: Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflow
    WEBINAR: Generation of purified human iPSC derived cardiomyocytes...
    DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
    Speaker: Todd Herron, BS, PhD
    Sponsored By: Miltenyi Biotec
    5 1
    FEB 23, 2017 | 12:00 PM
    Recent progress in cellular medicine with an eye to precision medicine
    Recent progress in cellular medicine with an eye to precision...
    Despite vast investment for decades in cancer research and development, cancer is still among the leading causes of death worldwide and its toll is expected to rise by about 70% over the next...
    Speaker: Giacinto Scoles, PhD
    Presented at: Precision Medicine Virtual Event Series 2017
    2
     
     
  • OCT 18, 2016 | 1:00 PM
    The Role of 3D Culture Methods in Retaining Native Biological Properties of Translational In Vitro Models
    The Role of 3D Culture Methods in Retaining Native Biological...
    Cell Sciences Innovation, Merck Research Laboratories, 2015 Galloping Hill Road, Kenilworth, NJ USA In vitro translational models play a critical role in the drug discovery process because t...
    Speaker: Richard Visconti, PhD
    Presented at: SyncD³ - Where drug discovery & development meet
    0
    OCT 05, 2016 | 7:30 AM
    Keynote Speaker: How to meet the challenge of counting and analyzing tumor cells circulating in the peripheral blood
    Keynote Speaker: How to meet the challenge of counting and analyzing...
    The talk will first report on a new patented method of counting CTCs based on the metabolism of tumor cells, that favors acidification of their near environment. In order to maintain the acid...
    Speaker: Giacinto Scoles, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    3
    SEP 20, 2016 | 8:00 AM
    Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions
    Use of Surface Plasmon Resonance for Probing Cell-Matrix Interactions
    DATE: September 20, 2016 TIME: 8:00am PST, 11:00 EST Studies of cell-substrate interactions are important for an array of biotechnology applications.  The nature of specific, recepto...
    Speaker: Michael Hill
    Sponsored By: Ametek, Ametek , Ametek
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