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    Primary Culture

    Primary Culture: the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate.

    Webinars

    • SEP 10, 2019 | 3:00 PM
      From cell seeding to analysis - Getting the best out of your cell-based assay
      From cell seeding to analysis - Getting the best out of your cell-based...
      DATE: September 10, 2019 TIME: 3:00pm CEST, 06:00am PT, 09:00am ET The assessment of cell health and cellular responses after experimental manipulation continue to be...
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf, Promega
      16
      SEP 05, 2019 | 12:00 PM
      Advancing astrocyte research with novel tools for characterization, isolation, and cultivation
      Advancing astrocyte research with novel tools for characterization,...
      DATE: September 5, 2019TIME: 12:00pm PDTAstrocytes are a major cell type in the mammalian CNS. They are involved in a number of essential processes such as synapse formation and functio...
      Speaker: Melanie Jungblut, Ph.D
      Sponsored By: Miltenyi Biotec
      4
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
      AUG 22, 2019 | 6:00 AM
      In Vitro Screening Approaches to Estimate Human Hepatic Responses to Xenobiotic Exposures
      In Vitro Screening Approaches to Estimate Human Hepatic Responses...
      Prediction of human response to chemical exposures is the primary challenge of pharmaceutical and environmental toxicology research. The complexity of human responses to xenobiotic exposures...
      Speaker: Stephen Ferguson, PhD
      Presented at: Forensics & Toxicology 2019
      1
      AUG 15, 2019 | 9:00 AM
      Understanding and ameliorating radiation-induced damage to oligodendrocytes
      Understanding and ameliorating radiation-induced damage to oligodendrocytes
      DATE: August 15, 2019TIME: 9:00am PDT, 12:00pm EDT Radiation therapy is a critical tool for the treatment of brain tumors, however, exposure to high doses of ionizing radiation...
      Speaker: Alexandra Taraboletti, PHD
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      JUL 31, 2019 | 9:00 AM
      Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
      Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
      DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
      Speaker: Elizabeth Delery, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      7 1
      JUN 27, 2019 | 9:00 AM
      CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system for studying cellular microenvironment
      CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system...
      DATE: June 27, 2019TIME: 9:00am PDT, 12:00pm EDT At present cancer research focuses on three major areas viz. cancer diagnostics, drugs development, and next-generation therapi...
      Speaker: Vivek Kamat, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      12
      JUN 19, 2019 | 10:00 AM
      Getting started in 3D:  Ask an Expert
      Getting started in 3D: Ask an Expert
      DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
      Speaker: Mark Kennedy, Ph.D. , Richard Josephson, PhD
      Sponsored By: Thermo Fisher Scientific
      6
      JUN 18, 2019 | 4:00 PM
      Pluripotent stem cells:  How to raise the quality of your PSC culture
      Pluripotent stem cells: How to raise the quality of your PSC...
      DATE: June 18, 2019TIME: 7:00am PDT, 10:00 EDT, 4:00pm CET PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug...
      Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
      Sponsored By: Eppendorf
      13
      JUN 05, 2019 | 5:00 PM
      Everyday culture practice - Improving reproducibility in cell culture
      Everyday culture practice - Improving reproducibility in cell...
      DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
      Speaker: Jessica Wagener, PhD
      Sponsored By: Eppendorf
      23 2
      MAY 16, 2019 | 4:00 PM
      Towards Standardized Workflows - A focus on microbiome insights
      Towards Standardized Workflows - A focus on microbiome insights
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
      Sponsored By: Eppendorf
      10 2
      APR 23, 2019 | 11:00 AM
      Utilizing the lens epithelial explant culture system to investigate cataract formation
      Utilizing the lens epithelial explant culture system to investigate...
      DATE: April 23, 2019TIME: 11:00am PDT, 2:00pm EDT Cataract, a clouding of the ocular lens, is the leading cause of blindness worldwide. Currently the only means of treatment i...
      Speaker: Daisy Shu, B.Optom, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      3
      APR 17, 2019 | 9:00 AM
      You've cultured an organoid and spheroid... now what?
      You've cultured an organoid and spheroid... now what?
      DATE:  April 17, 2019TIME:  9:00am PDT...
      Speaker: Takanori Takebe
      Sponsored By: Thermo Fisher Scientific
      5
      APR 03, 2019 | 9:00 AM
      How to analyze/characterize your 3D culture
      How to analyze/characterize your 3D culture
      DATE:  April 3, 2019TIME:  9:00am PDT...
      Speaker: Chris Langsdorf
      Sponsored By: Thermo Fisher Scientific
      6
      MAR 20, 2019 | 9:00 AM
      Getting started in 3D - Setting up your lab and reviewing common pitfalls of researchers new to 3D
      Getting started in 3D - Setting up your lab and reviewing common...
      DATE:  March 20, 2019TIME:  9:00am PDT...
      Speaker: Mark Kennedy, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      7
      MAR 07, 2019 | 7:00 AM
      Pluripotent stem cells:  How to raise the quality of your PSC culture
      Pluripotent stem cells: How to raise the quality of your PSC...
      DATE:    March 7, 2019TIME:    4:00pm CET PSCs represent an important tool in a wide range of applications, including basic research, disease...
      Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
      Sponsored By: Eppendorf
      4
      MAR 06, 2019 | 11:00 PM
      Pluripotent stem cells: How to raise the quality of your PSC culture
      Pluripotent stem cells: How to raise the quality of your PSC culture
      DATE:    March 7, 2019TIME:    8:00am CET PSCs represent an important tool in a wide range of applications, including basic research, disease...
      Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
      Sponsored By: Eppendorf
      5
      FEB 27, 2019 | 1:30 PM
      Advances in Three-Dimensional Cell Culture in Drug Research and Discovery
      Advances in Three-Dimensional Cell Culture in Drug Research and...
      Cellular research is optimal when using physiologically-relevant cell phenotypes and genotypes of human origin. This assertion has accelerated the adoption of primary cells, stem cells, and i...
      Speaker: Richard Eglen, PhD
      Presented at: Drug Discovery Virtual Event Series 2019
      2
      FEB 27, 2019 | 1:30 PM
      Advanced Liver Tissue Model for Improved in Vitro Prediction
      Advanced Liver Tissue Model for Improved in Vitro Prediction
      Isolated hepatocytes or their enzymes comprise the basis of most in vitro DMPK and toxicity assays used to predict human hepatic outcomes. Because hepato-specific functions are typically lost...
      Speaker: Matthew Shipton, MS
      Presented at: Drug Discovery Virtual Event Series 2019
      1
      FEB 27, 2019 | 8:00 AM
      Prepare for success: GMP cell banks as part of a staged, standardized, platform-style cell production process
      Prepare for success: GMP cell banks as part of a staged, standardized,...
      DATE:  February 27, 2019TIME:  8:00am PST Pluripotent stem cells (PSCs) can form any tissue or cell in the body, and are the ideal starting material to manufacture...
      Speaker: Benjamin Fryer
      Sponsored By: Thermo Fisher Scientific/Gibco
      9
      FEB 26, 2019 | 11:00 AM
      Osteosarcoma research
      Osteosarcoma research
      DATE:  February 26, 2019TIME:  11:00am PST, 2:00pm EST Osteosarcoma is the most common type of primary bone cancer affecting adolescents and children attributed to...
      Speaker: Luke Tattersall, BSc Biomedical Sciences, PhD Candidate
      Sponsored By: Thermo Fisher Scientific/Gibco
      3
      FEB 20, 2019 | 9:00 AM
      Choosing the best system to answer your question - What question are you looking to answer?
      Choosing the best system to answer your question - What question...
      DATE:  February 20, 2019TIME:  9:00am PST  ...
      Speaker: David Kuninger, PhD
      Sponsored By: Thermo Fisher Scientific
      15 1
      JAN 31, 2019 | 1:30 PM
      Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications
      Standardization of Cell Viability Assays in Primary Cells as a...
      Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and ne...
      Speaker: Simon Kaja, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      2
      DEC 06, 2018 | 9:00 AM
      Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows
      Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows
      DATE:   December 6, 2018TIME:    9:00am PST, 12:00pm EST Induced pluripotent stem cells (iPSC) intended for translational applications need to be of...
      Speaker: Tia Hexom, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
    SEP 10, 2019 | 3:00 PM
    From cell seeding to analysis - Getting the best out of your cell-based assay
    From cell seeding to analysis - Getting the best out of your cell-based...
    DATE: September 10, 2019 TIME: 3:00pm CEST, 06:00am PT, 09:00am ET The assessment of cell health and cellular responses after experimental manipulation continue to be...
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf, Promega
    16
    SEP 05, 2019 | 12:00 PM
    Advancing astrocyte research with novel tools for characterization, isolation, and cultivation
    Advancing astrocyte research with novel tools for characterization,...
    DATE: September 5, 2019TIME: 12:00pm PDTAstrocytes are a major cell type in the mammalian CNS. They are involved in a number of essential processes such as synapse formation and functio...
    Speaker: Melanie Jungblut, Ph.D
    Sponsored By: Miltenyi Biotec
    4
    SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
    17 1
    AUG 22, 2019 | 6:00 AM
    In Vitro Screening Approaches to Estimate Human Hepatic Responses to Xenobiotic Exposures
    In Vitro Screening Approaches to Estimate Human Hepatic Responses...
    Prediction of human response to chemical exposures is the primary challenge of pharmaceutical and environmental toxicology research. The complexity of human responses to xenobiotic exposures...
    Speaker: Stephen Ferguson, PhD
    Presented at: Forensics & Toxicology 2019
    1
    AUG 15, 2019 | 9:00 AM
    Understanding and ameliorating radiation-induced damage to oligodendrocytes
    Understanding and ameliorating radiation-induced damage to oligodendrocytes
    DATE: August 15, 2019TIME: 9:00am PDT, 12:00pm EDT Radiation therapy is a critical tool for the treatment of brain tumors, however, exposure to high doses of ionizing radiation...
    Speaker: Alexandra Taraboletti, PHD
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
     
     
  • JUL 31, 2019 | 9:00 AM
    Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
    Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
    DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
    Speaker: Elizabeth Delery, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    7 1
    JUN 27, 2019 | 9:00 AM
    CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system for studying cellular microenvironment
    CANCER ON A CHIP: A microfluidic 2D and 3D cell culture system...
    DATE: June 27, 2019TIME: 9:00am PDT, 12:00pm EDT At present cancer research focuses on three major areas viz. cancer diagnostics, drugs development, and next-generation therapi...
    Speaker: Vivek Kamat, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    12
    JUN 19, 2019 | 10:00 AM
    Getting started in 3D:  Ask an Expert
    Getting started in 3D: Ask an Expert
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    Speaker: Mark Kennedy, Ph.D. , Richard Josephson, PhD
    Sponsored By: Thermo Fisher Scientific
    6
    JUN 18, 2019 | 4:00 PM
    Pluripotent stem cells:  How to raise the quality of your PSC culture
    Pluripotent stem cells: How to raise the quality of your PSC...
    DATE: June 18, 2019TIME: 7:00am PDT, 10:00 EDT, 4:00pm CET PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug...
    Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
    Sponsored By: Eppendorf
    13
    JUN 05, 2019 | 5:00 PM
    Everyday culture practice - Improving reproducibility in cell culture
    Everyday culture practice - Improving reproducibility in cell...
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    Speaker: Jessica Wagener, PhD
    Sponsored By: Eppendorf
    23 2
     
     
  • MAY 16, 2019 | 4:00 PM
    Towards Standardized Workflows - A focus on microbiome insights
    Towards Standardized Workflows - A focus on microbiome insights
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
    Sponsored By: Eppendorf
    10 2
    APR 23, 2019 | 11:00 AM
    Utilizing the lens epithelial explant culture system to investigate cataract formation
    Utilizing the lens epithelial explant culture system to investigate...
    DATE: April 23, 2019TIME: 11:00am PDT, 2:00pm EDT Cataract, a clouding of the ocular lens, is the leading cause of blindness worldwide. Currently the only means of treatment i...
    Speaker: Daisy Shu, B.Optom, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    3
    APR 17, 2019 | 9:00 AM
    You've cultured an organoid and spheroid... now what?
    You've cultured an organoid and spheroid... now what?
    DATE:  April 17, 2019TIME:  9:00am PDT...
    Speaker: Takanori Takebe
    Sponsored By: Thermo Fisher Scientific
    5
    APR 03, 2019 | 9:00 AM
    How to analyze/characterize your 3D culture
    How to analyze/characterize your 3D culture
    DATE:  April 3, 2019TIME:  9:00am PDT...
    Speaker: Chris Langsdorf
    Sponsored By: Thermo Fisher Scientific
    6
    MAR 20, 2019 | 9:00 AM
    Getting started in 3D - Setting up your lab and reviewing common pitfalls of researchers new to 3D
    Getting started in 3D - Setting up your lab and reviewing common...
    DATE:  March 20, 2019TIME:  9:00am PDT...
    Speaker: Mark Kennedy, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    7
     
     
  • MAR 07, 2019 | 7:00 AM
    Pluripotent stem cells:  How to raise the quality of your PSC culture
    Pluripotent stem cells: How to raise the quality of your PSC...
    DATE:    March 7, 2019TIME:    4:00pm CET PSCs represent an important tool in a wide range of applications, including basic research, disease...
    Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
    Sponsored By: Eppendorf
    4
    MAR 06, 2019 | 11:00 PM
    Pluripotent stem cells: How to raise the quality of your PSC culture
    Pluripotent stem cells: How to raise the quality of your PSC culture
    DATE:    March 7, 2019TIME:    8:00am CET PSCs represent an important tool in a wide range of applications, including basic research, disease...
    Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
    Sponsored By: Eppendorf
    5
    FEB 27, 2019 | 1:30 PM
    Advances in Three-Dimensional Cell Culture in Drug Research and Discovery
    Advances in Three-Dimensional Cell Culture in Drug Research and...
    Cellular research is optimal when using physiologically-relevant cell phenotypes and genotypes of human origin. This assertion has accelerated the adoption of primary cells, stem cells, and i...
    Speaker: Richard Eglen, PhD
    Presented at: Drug Discovery Virtual Event Series 2019
    2
    FEB 27, 2019 | 1:30 PM
    Advanced Liver Tissue Model for Improved in Vitro Prediction
    Advanced Liver Tissue Model for Improved in Vitro Prediction
    Isolated hepatocytes or their enzymes comprise the basis of most in vitro DMPK and toxicity assays used to predict human hepatic outcomes. Because hepato-specific functions are typically lost...
    Speaker: Matthew Shipton, MS
    Presented at: Drug Discovery Virtual Event Series 2019
    1
    FEB 27, 2019 | 8:00 AM
    Prepare for success: GMP cell banks as part of a staged, standardized, platform-style cell production process
    Prepare for success: GMP cell banks as part of a staged, standardized,...
    DATE:  February 27, 2019TIME:  8:00am PST Pluripotent stem cells (PSCs) can form any tissue or cell in the body, and are the ideal starting material to manufacture...
    Speaker: Benjamin Fryer
    Sponsored By: Thermo Fisher Scientific/Gibco
    9
     
     
  • FEB 26, 2019 | 11:00 AM
    Osteosarcoma research
    Osteosarcoma research
    DATE:  February 26, 2019TIME:  11:00am PST, 2:00pm EST Osteosarcoma is the most common type of primary bone cancer affecting adolescents and children attributed to...
    Speaker: Luke Tattersall, BSc Biomedical Sciences, PhD Candidate
    Sponsored By: Thermo Fisher Scientific/Gibco
    3
    FEB 20, 2019 | 9:00 AM
    Choosing the best system to answer your question - What question are you looking to answer?
    Choosing the best system to answer your question - What question...
    DATE:  February 20, 2019TIME:  9:00am PST  ...
    Speaker: David Kuninger, PhD
    Sponsored By: Thermo Fisher Scientific
    15 1
    JAN 31, 2019 | 1:30 PM
    Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications
    Standardization of Cell Viability Assays in Primary Cells as a...
    Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and ne...
    Speaker: Simon Kaja, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    2
    DEC 06, 2018 | 9:00 AM
    Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows
    Cell and Gene Therapy Solutions: Enabling PSC Translation Workflows
    DATE:   December 6, 2018TIME:    9:00am PST, 12:00pm EST Induced pluripotent stem cells (iPSC) intended for translational applications need to be of...
    Speaker: Tia Hexom, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
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