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    RNA Isolation

    RNA isolation from cells or tissues is the first step in a number of routine laboratory experiments to assess gene expression levels and is used in assays including quantitative PCR, microarray analysis, and Northern blots. RNA isolation therefore requires cautious handling of samples and good aseptic techniques.

    Webinars

    • SEP 27, 2017 | 7:30 AM
      Keynote Presentation: What Microscopy Can Tell Us About the Life of an mRNA in Living Cells
      Keynote Presentation: What Microscopy Can Tell Us About the Life...
      Einstein researcher Robert Singer, Ph.D., discusses a breakthrough in microscopy that is allowing scientists to track messenger RNA in living cells in real time. The study, published in the S...
      Speaker: Robert Singer, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 6:00 AM
      Regulation of microtubule plus-end dynamics during axon guidance and cell migration
      Regulation of microtubule plus-end dynamics during axon guidance...
      A fundamental question in neuronal development is how growth cone cytoskeletal dynamics are coordinated to promote accurate axonal navigation. To address this question, we focus on microtubul...
      Speaker: Laura Lowery, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 14, 2017 | 6:00 AM
      A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: taxonomic and evolutionary considerations
      A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in...
      A rapidly growing number of viruses of lower eukaryotes have been reported in the past few decades. These have enhanced our understanding of virus evolution and diversity. Simultaneously, som...
      Speaker: Nobuhiro Suzuki, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      3
      SEP 13, 2017 | 12:00 PM
      High Resolution Outbreak Tracing and Resistance Detection using Whole Genome Sequencing in the case of a Mycobacterium tuberculosis Outbreak
      High Resolution Outbreak Tracing and Resistance Detection using...
      Background: In March 2014, a molecular cluster of five multidrug resistant Mycobacterium tuberculosis was detected by the Austrian National Reference Laboratory. An investigation was initiate...
      Speaker: Winnie Ridderberg, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      1
      SEP 13, 2017 | 7:30 AM
      Keynote Presentation: Using phage to select for evolution of reduced virulence in pathogenic bacteria
      Keynote Presentation: Using phage to select for evolution of reduced...
      Increasing prevalence and severity of multi-drug-resistant (MDR) bacterial infections has necessitated novel antibacterial strategies.  Ideally, new approaches would target bacterial pat...
      Speaker: Paul Turner, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      1
      JUL 01, 2017 | 8:00 AM
      Genomic profiling reveals novel PAX7 targets CD54, integrin α9β1 and SDC2, as markers for isolation of human ES/iPS cell-derived muscle progenitors
      Genomic profiling reveals novel PAX7 targets CD54, integrin α9β1...
      Therapeutic application of pluripotent stem (PS) cell-derived products represents the ultimate goal of stem cell research. In order to apply this technology to patients, it is fundamental to ...
      Speaker: Alessandro Magli
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      1
      JUL 01, 2017 | 12:00 AM
      New Tools for improved CRISPR gene-editing in stem cells
      New Tools for improved CRISPR gene-editing in stem cells
      The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
      Speaker: James Kehler VMD, PhD
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      2
      JUN 08, 2017 | 12:00 PM
      Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing the Latest Technologies from Thermo Fisher Scientific
      Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing...
      The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
      Speaker: Rhonda Newman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      3
      APR 25, 2017 | 10:00 AM
      WEBINAR: Discover Potentially Predictive Immuno-Oncology Biomarkers using Next-Gen Sequencing
      WEBINAR: Discover Potentially Predictive Immuno-Oncology Biomarkers...
      DATE: April 25, 2017TIME: 10:00am PT, 1:00pm ETImmunotherapy has emerged as one of the most promising options for the future treatment of a growing number of cancers and is to...
      Speaker: Fiona Hyland , Carl Morrison MD, DVM
      Sponsored By: Thermo Fisher Scientific - Ion Torrent
      6 1
      SEP 08, 2016 | 7:30 AM
      Gene Expression Profiling During Infection NanoString® nCounter accurately quantifies pathogen and host transcripts from tiny amount of infection tissue
      Gene Expression Profiling During Infection NanoString® nCounter...
      Our speaker, Wenjie Xu, Ph.D., will present his publication data demonstrating how nCounter® technology can advance your infectious disease research faster and more accurately with unprec...
      Speaker: Wenjie Xu, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2016
      2
      JUN 09, 2016 | 9:00 AM
      Standardizing Microbiomics
      Standardizing Microbiomics
      DATE:  June 9, 2016 TIME:   9AM Pacific time, 12pm Eastern time The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and...
      Speaker: Ryan Kemp, M.S. , Shuiquan Tang, PhD
      Sponsored By: Zymo Research, Zymo Research
      13
      JUN 02, 2016 | 8:00 AM
      WEBINAR: CE-SELEX:  Isolating High Affinity Aptamers Using Capillary Electrophoresis
      WEBINAR: CE-SELEX: Isolating High Affinity Aptamers Using Capillary...
           SELEX is a process that selects DNA or RNA from a random library of sequences based on their affinity for a target molecule.  These high affinity ligands, called apta...
      Speaker: Michael Bowser, PhD
      Sponsored By: Sciex, Sciex
      4 1
      MAR 15, 2016 | 10:00 AM
      Gene Expression Analysis Using 3'-RNA Sequencing
      Gene Expression Analysis Using 3'-RNA Sequencing
      10:00AM PT, 12:00PM CT, 1:00PM ET RNA sequencing (RNA-seq) has revolutionized the study of gene expression in animals, plants and microorganisms. However, because of its high cost, this te...
      Speaker: Behnam Abasht, PhD
      Sponsored By: Lexogen, Lexogen
      15 1
      NOV 17, 2015 | 8:00 AM
      Unleashing the power of biobanks to drive translational science forward
      Unleashing the power of biobanks to drive translational science...
      DATE: November 17th, 2015TIME: 8am PDT, 11am EDT, 4pm GMTBiobanks provide important capacity to collect large genomic data sets intended to enable the study of genetic signatures of an...
      Speaker: Conny Mathay , Andrew Brooks, Ph.D. , Jens Hoefkens, PhD
      Sponsored By: Revvity
      22
      SEP 03, 2015 | 12:00 PM
      Applying the MinION: microbial genomics and complex metagenomics
      Applying the MinION: microbial genomics and complex metagenomics
      Oxford Nanopore's MinION is a small, portable USB-powered sensing device which is powered by nanopore technology. It is adaptable to the analysis of DNA, RNA, proteins or small molecules, and...
      Speaker: Daniel Turner, PhD
      Presented at: Microbiology Virtual Event Series 2015
      6
      SEP 02, 2015 | 6:00 AM
      Simple, fast and accurate microbial identification by sequencing
      Simple, fast and accurate microbial identification by sequencing
      Managing microbial contaminants is critical during the manufacturing of biologics, food and beverage to ensure that products meet the high quality product standard as well as staying complian...
      Speaker: Jane Luo, PhD
      Presented at: Microbiology Virtual Event Series 2015
      9
      JUN 25, 2015 | 8:00 AM
      Large scale purification of RNA and RNA-based Nanoparticles by Preparative Ultracentrifugation
      Large scale purification of RNA and RNA-based Nanoparticles by...
      The emerging field of RNA nanotechnology has led to the utilization of the platform in the field of bionanotechnology as sensing platforms, diverse nanoparticle construction, in vivo computin...
      Speaker: Daniel Jasinski
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      7
      MAY 13, 2015 | 6:00 AM
      Challenges and New Solutions for Isolating Exosomes, Other Extracellular Vesicles and Vesicular RNAs
      Challenges and New Solutions for Isolating Exosomes, Other Extracellular...
      A new affinity-based spin column procedure has been developed for highly specific isolation of exosomes and other extracellular vesicles (EVs) from serum or plasma, as well as for vesicular R...
      Speaker: Martin Schlumpberger, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2015
      4
      JAN 27, 2015 | 2:00 AM
      Catch-and-Release: Extraction and Purification of NGS-Grade DNA and RNA from FFPE Tissue Samples
      Catch-and-Release: Extraction and Purification of NGS-Grade DNA...
      Formalin Fixed, Paraffin Embedded (FFPE) tissues are rapidly being adopted for transcriptome analysis in conjunction with targeted and whole genome sequencing. Harsh formaldehyde fixation an...
      Speaker: Guillaume Durin, PhD
      Sponsored By: Covaris, Inc., Covaris, Inc.
      10
      DEC 16, 2014 | 8:00 AM
      Detection of RNAs with flow cytometry
      Detection of RNAs with flow cytometry
       Single-cell analysis is one of the most rapidly growing fields in biomedical research that is significantly expanding our understanding on the biologic characteristics of various diseases. F...
      Speaker: Filippos Porichis, PhD
      Sponsored By: eBioscience, an Affymetrix business, eBioscience, an Affymetrix business
      79
      OCT 30, 2014 | 9:00 AM
      A head-to-head comparison of whole blood derived samples (cfDNA vs CTC DNA) for cancer research using next-generation sequencing
      A head-to-head comparison of whole blood derived samples (cfDNA...
      Both cell free DNA (cfDNA) and circulating tumor cells (CTC) represent important possible templates for mutation analysis of clinical samples. Each template has different theoretical advantag...
      Speaker: Paul W. Dempsey, PhD
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
      0
      OCT 29, 2014 | 9:00 AM
      miRNA Biomarker Discovery: Technologies, workflows, and data analysis solutions for discovering your unique signature
      miRNA Biomarker Discovery: Technologies, workflows, and data analysis...
      Circulating miRNAs have great potential as biomarkers due to their aberrant expression in cancer and other diseases. However, miRNAs from body fluids are hard to obtain in amounts sufficient...
      Speaker: Jonathan Shaffer, PhD, MBA
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
      18
      AUG 20, 2014 | 8:45 AM
      Digitizing Life Using Synthetic Genomics
      Digitizing Life Using Synthetic Genomics
      In 2010, our team of synthetic biologists announced the creation of a bacterial cell that had a chemically synthesized genome. To build this synthetic Mycoplasma mycoides JCVI 1.0 we had to d...
      Speaker: John Glass, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2014
      135
      OCT 17, 2013 | 7:00 AM
      Advancing Translational Cancer Research through Automated RNASeq Sample Preparation from Archival FFPE Tissue Specimens
      Advancing Translational Cancer Research through Automated RNASeq...
      RNA-Seq allows the simultaneous observation of gene expression levels, mutations in the coding sequences, splice variants and gene fusions, which are especially important in cancer studies. A...
      Speaker: Michaela Bowden, PhD
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2013
      98
    SEP 27, 2017 | 7:30 AM
    Keynote Presentation: What Microscopy Can Tell Us About the Life of an mRNA in Living Cells
    Keynote Presentation: What Microscopy Can Tell Us About the Life...
    Einstein researcher Robert Singer, Ph.D., discusses a breakthrough in microscopy that is allowing scientists to track messenger RNA in living cells in real time. The study, published in the S...
    Speaker: Robert Singer, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 6:00 AM
    Regulation of microtubule plus-end dynamics during axon guidance and cell migration
    Regulation of microtubule plus-end dynamics during axon guidance...
    A fundamental question in neuronal development is how growth cone cytoskeletal dynamics are coordinated to promote accurate axonal navigation. To address this question, we focus on microtubul...
    Speaker: Laura Lowery, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 14, 2017 | 6:00 AM
    A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: taxonomic and evolutionary considerations
    A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in...
    A rapidly growing number of viruses of lower eukaryotes have been reported in the past few decades. These have enhanced our understanding of virus evolution and diversity. Simultaneously, som...
    Speaker: Nobuhiro Suzuki, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    3
    SEP 13, 2017 | 12:00 PM
    High Resolution Outbreak Tracing and Resistance Detection using Whole Genome Sequencing in the case of a Mycobacterium tuberculosis Outbreak
    High Resolution Outbreak Tracing and Resistance Detection using...
    Background: In March 2014, a molecular cluster of five multidrug resistant Mycobacterium tuberculosis was detected by the Austrian National Reference Laboratory. An investigation was initiate...
    Speaker: Winnie Ridderberg, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    1
    SEP 13, 2017 | 7:30 AM
    Keynote Presentation: Using phage to select for evolution of reduced virulence in pathogenic bacteria
    Keynote Presentation: Using phage to select for evolution of reduced...
    Increasing prevalence and severity of multi-drug-resistant (MDR) bacterial infections has necessitated novel antibacterial strategies.  Ideally, new approaches would target bacterial pat...
    Speaker: Paul Turner, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    1
     
     
  • JUL 01, 2017 | 8:00 AM
    Genomic profiling reveals novel PAX7 targets CD54, integrin α9β1 and SDC2, as markers for isolation of human ES/iPS cell-derived muscle progenitors
    Genomic profiling reveals novel PAX7 targets CD54, integrin α9β1...
    Therapeutic application of pluripotent stem (PS) cell-derived products represents the ultimate goal of stem cell research. In order to apply this technology to patients, it is fundamental to ...
    Speaker: Alessandro Magli
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    1
    JUL 01, 2017 | 12:00 AM
    New Tools for improved CRISPR gene-editing in stem cells
    New Tools for improved CRISPR gene-editing in stem cells
    The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
    Speaker: James Kehler VMD, PhD
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    2
    JUN 08, 2017 | 12:00 PM
    Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing the Latest Technologies from Thermo Fisher Scientific
    Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing...
    The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
    Speaker: Rhonda Newman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    3
    APR 25, 2017 | 10:00 AM
    WEBINAR: Discover Potentially Predictive Immuno-Oncology Biomarkers using Next-Gen Sequencing
    WEBINAR: Discover Potentially Predictive Immuno-Oncology Biomarkers...
    DATE: April 25, 2017TIME: 10:00am PT, 1:00pm ETImmunotherapy has emerged as one of the most promising options for the future treatment of a growing number of cancers and is to...
    Speaker: Fiona Hyland , Carl Morrison MD, DVM
    Sponsored By: Thermo Fisher Scientific - Ion Torrent
    6 1
    SEP 08, 2016 | 7:30 AM
    Gene Expression Profiling During Infection NanoString® nCounter accurately quantifies pathogen and host transcripts from tiny amount of infection tissue
    Gene Expression Profiling During Infection NanoString® nCounter...
    Our speaker, Wenjie Xu, Ph.D., will present his publication data demonstrating how nCounter® technology can advance your infectious disease research faster and more accurately with unprec...
    Speaker: Wenjie Xu, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2016
    2
     
     
  • JUN 09, 2016 | 9:00 AM
    Standardizing Microbiomics
    Standardizing Microbiomics
    DATE:  June 9, 2016 TIME:   9AM Pacific time, 12pm Eastern time The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and...
    Speaker: Ryan Kemp, M.S. , Shuiquan Tang, PhD
    Sponsored By: Zymo Research, Zymo Research
    13
    JUN 02, 2016 | 8:00 AM
    WEBINAR: CE-SELEX:  Isolating High Affinity Aptamers Using Capillary Electrophoresis
    WEBINAR: CE-SELEX: Isolating High Affinity Aptamers Using Capillary...
         SELEX is a process that selects DNA or RNA from a random library of sequences based on their affinity for a target molecule.  These high affinity ligands, called apta...
    Speaker: Michael Bowser, PhD
    Sponsored By: Sciex, Sciex
    4 1
    MAR 15, 2016 | 10:00 AM
    Gene Expression Analysis Using 3'-RNA Sequencing
    Gene Expression Analysis Using 3'-RNA Sequencing
    10:00AM PT, 12:00PM CT, 1:00PM ET RNA sequencing (RNA-seq) has revolutionized the study of gene expression in animals, plants and microorganisms. However, because of its high cost, this te...
    Speaker: Behnam Abasht, PhD
    Sponsored By: Lexogen, Lexogen
    15 1
    NOV 17, 2015 | 8:00 AM
    Unleashing the power of biobanks to drive translational science forward
    Unleashing the power of biobanks to drive translational science...
    DATE: November 17th, 2015TIME: 8am PDT, 11am EDT, 4pm GMTBiobanks provide important capacity to collect large genomic data sets intended to enable the study of genetic signatures of an...
    Speaker: Conny Mathay , Andrew Brooks, Ph.D. , Jens Hoefkens, PhD
    Sponsored By: Revvity
    22
    SEP 03, 2015 | 12:00 PM
    Applying the MinION: microbial genomics and complex metagenomics
    Applying the MinION: microbial genomics and complex metagenomics
    Oxford Nanopore's MinION is a small, portable USB-powered sensing device which is powered by nanopore technology. It is adaptable to the analysis of DNA, RNA, proteins or small molecules, and...
    Speaker: Daniel Turner, PhD
    Presented at: Microbiology Virtual Event Series 2015
    6
     
     
  • SEP 02, 2015 | 6:00 AM
    Simple, fast and accurate microbial identification by sequencing
    Simple, fast and accurate microbial identification by sequencing
    Managing microbial contaminants is critical during the manufacturing of biologics, food and beverage to ensure that products meet the high quality product standard as well as staying complian...
    Speaker: Jane Luo, PhD
    Presented at: Microbiology Virtual Event Series 2015
    9
    JUN 25, 2015 | 8:00 AM
    Large scale purification of RNA and RNA-based Nanoparticles by Preparative Ultracentrifugation
    Large scale purification of RNA and RNA-based Nanoparticles by...
    The emerging field of RNA nanotechnology has led to the utilization of the platform in the field of bionanotechnology as sensing platforms, diverse nanoparticle construction, in vivo computin...
    Speaker: Daniel Jasinski
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    7
    MAY 13, 2015 | 6:00 AM
    Challenges and New Solutions for Isolating Exosomes, Other Extracellular Vesicles and Vesicular RNAs
    Challenges and New Solutions for Isolating Exosomes, Other Extracellular...
    A new affinity-based spin column procedure has been developed for highly specific isolation of exosomes and other extracellular vesicles (EVs) from serum or plasma, as well as for vesicular R...
    Speaker: Martin Schlumpberger, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2015
    4
    JAN 27, 2015 | 2:00 AM
    Catch-and-Release: Extraction and Purification of NGS-Grade DNA and RNA from FFPE Tissue Samples
    Catch-and-Release: Extraction and Purification of NGS-Grade DNA...
    Formalin Fixed, Paraffin Embedded (FFPE) tissues are rapidly being adopted for transcriptome analysis in conjunction with targeted and whole genome sequencing. Harsh formaldehyde fixation an...
    Speaker: Guillaume Durin, PhD
    Sponsored By: Covaris, Inc., Covaris, Inc.
    10
    DEC 16, 2014 | 8:00 AM
    Detection of RNAs with flow cytometry
    Detection of RNAs with flow cytometry
     Single-cell analysis is one of the most rapidly growing fields in biomedical research that is significantly expanding our understanding on the biologic characteristics of various diseases. F...
    Speaker: Filippos Porichis, PhD
    Sponsored By: eBioscience, an Affymetrix business, eBioscience, an Affymetrix business
    79
     
     
  • OCT 30, 2014 | 9:00 AM
    A head-to-head comparison of whole blood derived samples (cfDNA vs CTC DNA) for cancer research using next-generation sequencing
    A head-to-head comparison of whole blood derived samples (cfDNA...
    Both cell free DNA (cfDNA) and circulating tumor cells (CTC) represent important possible templates for mutation analysis of clinical samples. Each template has different theoretical advantag...
    Speaker: Paul W. Dempsey, PhD
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
    0
    OCT 29, 2014 | 9:00 AM
    miRNA Biomarker Discovery: Technologies, workflows, and data analysis solutions for discovering your unique signature
    miRNA Biomarker Discovery: Technologies, workflows, and data analysis...
    Circulating miRNAs have great potential as biomarkers due to their aberrant expression in cancer and other diseases. However, miRNAs from body fluids are hard to obtain in amounts sufficient...
    Speaker: Jonathan Shaffer, PhD, MBA
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
    18
    AUG 20, 2014 | 8:45 AM
    Digitizing Life Using Synthetic Genomics
    Digitizing Life Using Synthetic Genomics
    In 2010, our team of synthetic biologists announced the creation of a bacterial cell that had a chemically synthesized genome. To build this synthetic Mycoplasma mycoides JCVI 1.0 we had to d...
    Speaker: John Glass, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2014
    135
    OCT 17, 2013 | 7:00 AM
    Advancing Translational Cancer Research through Automated RNASeq Sample Preparation from Archival FFPE Tissue Specimens
    Advancing Translational Cancer Research through Automated RNASeq...
    RNA-Seq allows the simultaneous observation of gene expression levels, mutations in the coding sequences, splice variants and gene fusions, which are especially important in cancer studies. A...
    Speaker: Michaela Bowden, PhD
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2013
    98
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