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Transfection
Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Classically, the term transfection was used to denote the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, resulting in an infection and the production of mature virus particles. However, the term has acquired its present meaning to include any artificial introduction of foreign nucleic acid into a cell.
NOV 15, 2018 | 1:00 PM
The Neon transfection system enables robust transfection efficiencies in hiPSC for DNA, mRNA and Cas9/gRNA RNP complexes. For hiPSC these improved transfection as well as post-electroporation...
OCT 03, 2018 | 12:00 PM
All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
OCT 03, 2018 | 9:00 AM
The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...
SEP 27, 2018 | 7:30 AM
Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...
SEP 26, 2018 | 12:00 PM
With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
SEP 18, 2018 | 8:00 AM
DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
MAY 16, 2018 | 10:00 PM
HEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系，可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料，以及使用293或CHO细胞时需要考虑的主要差异。...
MAY 16, 2018 | 8:00 PM
ExpiCHO是一种高效的瞬时表达系统，可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用，获得CHO表达蛋白必须构建稳定的细胞株，这一过程不仅耗时耗力，而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白，往往只能采用293细胞的瞬转表达系统，但该系统产量低，表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...