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    Genomics Selection

    Genomics Selection: is a form of marker-assisted selection in which genetic markers covering the whole genome are used so that all quantitative trait loci (QTL) are in linkage disequilibrium with at least one marker.

    Webinars

    • MAR 26, 2024 | 7:00 PM
      C.E. CREDITS
      Educational Webinar: Implementation of a Preemptive Pharmacogenomics Program at a Pediatric Health System
      Educational Webinar: Implementation of a Preemptive Pharmacogenomics...
      The implementation of a preemptive pharmacogenomics (PGx) program in a hospital setting requires a multidisciplinary approach to ensure seamless integration of each stage of the process for...
      Speaker: Steven Melnick. Ph.D., M.D. , Dr. David Mancuso, Pharm.D. CPh , MSPM
      Sponsored By: Thermo Fisher Scientific
      16 1
      MAR 26, 2024 | 8:00 AM
      C.E. CREDITS
      Educational Webinar: Implementation of a Preemptive Pharmacogenomics Program at a Pediatric Health System
      Educational Webinar: Implementation of a Preemptive Pharmacogenomics...
      The implementation of a preemptive pharmacogenomics (PGx) program in a hospital setting requires a multidisciplinary approach to ensure seamless integration of each stage of the process for...
      Speaker: Steven Melnick. Ph.D., M.D. , Dr. David Mancuso, Pharm.D. CPh , MSPM
      Sponsored By: Thermo Fisher Scientific
      22
      NOV 29, 2023 | 9:00 AM
      Labcorp® Plasma Focus™
      Labcorp® Plasma Focus™
      Tumor genomic profiling can be complicated, especially for diseases that commonly have multiple different genomic biomarkers of interest, such as non-small cell lung cancer (NSCLC) and color...
      Speaker: Mark Sausen, PhD , Mohamad Khasawneh, MD
      Sponsored By: Labcorp Oncology
      21
      OCT 31, 2023 | 11:00 AM
      Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes
      Optimizing Experimental Design for Gene Editing Success: tips...
      With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
      Speaker: Fuqiang Chen, Ph.D.
      Sponsored By: MilliporeSigma
      8
      OCT 10, 2023 | 10:00 AM
      C.E. CREDITS
      From Tissue to Insight: Genomic Testing Methodologies and Practical Considerations for Personalized Cancer Care
      From Tissue to Insight: Genomic Testing Methodologies and Practical...
      For patients with advanced non-small cell lung cancer (NSCLC), tissue samples are typically small biopsies. These samples undergo standard of care evaluation for >10 biomarkers to identif...
      Speaker: Kyle Strickland, MD, PhD , Rebecca Previs, MD
      Sponsored By: Labcorp Oncology
      29
      SEP 26, 2023 | 8:00 AM
      Exploring Extracellular Vesicles: Unlocking the Potential of EV Research
      Exploring Extracellular Vesicles: Unlocking the Potential of EV...
      Join us for an educational webinar that delves into the fascinating world of Extracellular Vesicles (EVs) and their impact on biomedical research and medicine. In collaboration with ExoVecto...
      Speaker: Roland Leathers, PhD , Jeroen de Vrij, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      41 1
      SEP 13, 2023 | 7:00 AM
      Developing genotyping platforms to enable discovery, quality control and genomic breeding at CoverCress Inc.
      Developing genotyping platforms to enable discovery, quality control...
      As the demand for renewal fuel feedstocks continues to grow, non-food feedstocks production must significantly increase to meet demand. Winter oilseeds grown in cooler climates provide oppor...
      Speaker: Ratan Chopra, PhD
      Sponsored By: LGC Biosearch Technologies
      17
      AUG 29, 2023 | 8:00 AM
      The Powers of Odin:  Comprehensive Cellular Characterization
      The Powers of Odin: Comprehensive Cellular Characterization
      Prokaryotic and eukaryotic cells change and adapt to their environments in ways that genomic characterization methods do not fully capture or predict. Even strains of the same species of mic...
      Speaker: Dina Finan, PhD
      Sponsored By: Biolog
      14
      JUL 26, 2023 | 4:05 PM
      Discovery and engineering of a miniature CRISPR-Cas type V-L system
      Discovery and engineering of a miniature CRISPR-Cas type V-L system
      Employing a metagenomic search, we identified a family of miniature CRISPR-Cas type V-L systems capable of RNA-guided dsDNA target cleavage without a tracrRNA. A bacterial depletion screen r...
      Speaker: Lauren Alfonse
      Presented at: GenScript 3rd Annual Gene and Cell Engineering Summit
      6
      MAR 01, 2023 | 8:00 AM
      Maximizing metastasis insights: Using a novel integrated workflow approach to achieve efficient CTC enrichment, enumeration and identification
      Maximizing metastasis insights: Using a novel integrated workflow...
      Date: March 01, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Characterizing metastasis remains key for understanding and eradicating cancer. The study of circulating tumor cells (CTC...
      Speaker: Adam Corner, Ph.D.
      Sponsored By: Bio-Rad Laboratories
      8
      FEB 22, 2023 | 9:00 AM
      A scalable miniature circular GenCircleâ„¢ dsDNA production system for gene and cell therapy
      A scalable miniature circular GenCircleâ„¢ dsDNA production system...
      Date: February 22, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) Circular plasmid DNA has traditionally been used as a stable and efficient vector for gene expression and HDR-mediated...
      Speaker: Dr. Fan Zhou, PhD
      Sponsored By: GenScript
      7
      JAN 18, 2023 | 9:00 AM
      Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
      Elucidating the composition of CRISPR-Cas12f1 complexes using...
      Date: January 18, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) CRISPR-Cas systems are widely used in genome engineering applications. They contain RNA-guided ribonucleoprotein (RNP)...
      Speaker: Selgar Henkel-Heinecke
      Sponsored By: Refeyn
      13
      OCT 11, 2022 | 8:00 AM
      Smarter sample preparation for single-cell sequencing
      Smarter sample preparation for single-cell sequencing
      Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
      Speaker: Carina Emery
      Sponsored By: Miltenyi Biotec
      22
      OCT 04, 2022 | 9:00 AM
      Enabling fully automated NGS sample preparation using digital microfluidics
      Enabling fully automated NGS sample preparation using digital...
      Date: October 04, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) The Miro Canvas is a new platform which enables full walkaway automation of complex next generation sequencing (NGS) s...
      Speaker: Dr. Jonathan Levine , Dr. Fay Christodoulou
      Sponsored By: Miroculus
      13
      SEP 27, 2022 | 8:00 AM
      Efficient and Rapid Discovery of Novel Temperature Sensitive Alleles with the Onyx® Digital Genome Engineering Platform
      Efficient and Rapid Discovery of Novel Temperature Sensitive Alleles...
      Date: September 27, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Temperature sensitive (TS) alleles are mutations in essential genes that allow for growth at permissive temperatures...
      Speaker: Randy Lacey
      Sponsored By: Inscripta
      10 2
      SEP 22, 2022 | 9:00 AM
      Human proteomics: from the operating room to the lab and back
      Human proteomics: from the operating room to the lab and back
      Date: September 22, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Optimizing platforms for surgical specimen collection and deep human phenotyping was used to enhance protein biomarke...
      Speaker: Vinit Mahajan M.D., Ph.D.
      Sponsored By: SomaLogic
      17
      SEP 15, 2022 | 8:00 AM
      Tools and partnerships for sustainably increasing rice production
      Tools and partnerships for sustainably increasing rice production
      Date: September 15, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Improving rice to withstand diseases, climate-related stresses and reduced inputs will contribute to food security a...
      Speaker: Dr. Katherine Steele , Darshna Vyas
      Sponsored By: LGC Biosearch Technologies
      10
      MAY 19, 2022 | 8:00 AM
      Automated sample preparation for reproducible clinical proteomics
      Automated sample preparation for reproducible clinical proteomics
      Date: May 19, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classific...
      Speaker: Jeroen Krijgsveld , Nicolas Autret, PhD , Ignasi Forné, PhD
      Sponsored By: Covaris, Inc.
      14
      APR 28, 2022 | 9:00 AM
      Beyond infection prevention and control: How genome sequencing of AMR pathogens can improve antibiotic stewardship and patient care
      Beyond infection prevention and control: How genome sequencing...
      Date: April 28, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CET) During previous webinars we have discussed the global impact of antimicrobial resistance (AMR) infections, explored biolo...
      Speaker: Dr. Arne Materna
      Sponsored By: OpGen
      11
      APR 26, 2022 | 7:00 AM
      High-Content Phenotypic Screening - Answering Questions You Haven't Even Asked Yet
      High-Content Phenotypic Screening - Answering Questions You Haven't...
      Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
      Speaker: Dr. Angeline Lim , Dr. Victor Wong
      Sponsored By: Molecular Devices
      5
      APR 20, 2022 | 5:30 AM
      Improving Fetal Fraction of EDTA-Gel NIPS Samples Using Gel-Based Size-Selection
      Improving Fetal Fraction of EDTA-Gel NIPS Samples Using Gel-Based...
      Objective : Evaluation of EDTA-gel blood collection tubes from remote locations for NIPS (Non-invasive prenatal testing). Design: Paired comparisons Population: 61 pregnant women from Britis...
      Speaker: Dr François Rousseau, MD, MSc, FRCPC, FCAHS
      Presented at: Genetics Week Virtual Event Series 2022
      3
      APR 05, 2022 | 9:00 AM
      Enterovirus membrane-assisted assembly and release revealed by cryo-electron tomography
      Enterovirus membrane-assisted assembly and release revealed by...
      Date: April 5, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases...
      Speaker: Selma Dahmane , Kristian Wadel
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific - Electron Microscopy
      7
      DEC 09, 2021 | 11:00 AM
      Surveillance and AI-Powered Prediction of AMR to Inform Antibiotic Therapy
      Surveillance and AI-Powered Prediction of AMR to Inform Antibiotic...
      Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
      Speaker: Dr. Arne Materna
      Sponsored By: OpGen
      7
      DEC 08, 2021 | 9:00 AM
      The MAD7â„¢ nuclease in plant editing: overcoming CRISPR bottlenecks with MAD TiGER
      The MAD7â„¢ nuclease in plant editing: overcoming CRISPR bottlenecks...
      Date: December 9, 2021 Time: 9:00am (PDT), 12:00am (EDT) CRISPR editing has revolutionized plant breeding, and the MAD7 CRISPR nuclease developed by InscriptaTM is democratizing access to CR...
      Speaker: Gabino Sanchez , Ben Mijts
      Sponsored By: Inscripta
      5
    MAR 26, 2024 | 7:00 PM
    C.E. CREDITS
    Educational Webinar: Implementation of a Preemptive Pharmacogenomics Program at a Pediatric Health System
    Educational Webinar: Implementation of a Preemptive Pharmacogenomics...
    The implementation of a preemptive pharmacogenomics (PGx) program in a hospital setting requires a multidisciplinary approach to ensure seamless integration of each stage of the process for...
    Speaker: Steven Melnick. Ph.D., M.D. , Dr. David Mancuso, Pharm.D. CPh , MSPM
    Sponsored By: Thermo Fisher Scientific
    16 1
    MAR 26, 2024 | 8:00 AM
    C.E. CREDITS
    Educational Webinar: Implementation of a Preemptive Pharmacogenomics Program at a Pediatric Health System
    Educational Webinar: Implementation of a Preemptive Pharmacogenomics...
    The implementation of a preemptive pharmacogenomics (PGx) program in a hospital setting requires a multidisciplinary approach to ensure seamless integration of each stage of the process for...
    Speaker: Steven Melnick. Ph.D., M.D. , Dr. David Mancuso, Pharm.D. CPh , MSPM
    Sponsored By: Thermo Fisher Scientific
    22
    NOV 29, 2023 | 9:00 AM
    Labcorp® Plasma Focus™
    Labcorp® Plasma Focus™
    Tumor genomic profiling can be complicated, especially for diseases that commonly have multiple different genomic biomarkers of interest, such as non-small cell lung cancer (NSCLC) and color...
    Speaker: Mark Sausen, PhD , Mohamad Khasawneh, MD
    Sponsored By: Labcorp Oncology
    21
    OCT 31, 2023 | 11:00 AM
    Optimizing Experimental Design for Gene Editing Success: tips and tricks to increase efficiency and improve outcomes
    Optimizing Experimental Design for Gene Editing Success: tips...
    With the promise of targeted gene editing and CRISPR technologies comes increasing experimental complexity and design challenges. Success in gene editing experiments may depend on many varia...
    Speaker: Fuqiang Chen, Ph.D.
    Sponsored By: MilliporeSigma
    8
    OCT 10, 2023 | 10:00 AM
    C.E. CREDITS
    From Tissue to Insight: Genomic Testing Methodologies and Practical Considerations for Personalized Cancer Care
    From Tissue to Insight: Genomic Testing Methodologies and Practical...
    For patients with advanced non-small cell lung cancer (NSCLC), tissue samples are typically small biopsies. These samples undergo standard of care evaluation for >10 biomarkers to identif...
    Speaker: Kyle Strickland, MD, PhD , Rebecca Previs, MD
    Sponsored By: Labcorp Oncology
    29
     
     
  • SEP 26, 2023 | 8:00 AM
    Exploring Extracellular Vesicles: Unlocking the Potential of EV Research
    Exploring Extracellular Vesicles: Unlocking the Potential of EV...
    Join us for an educational webinar that delves into the fascinating world of Extracellular Vesicles (EVs) and their impact on biomedical research and medicine. In collaboration with ExoVecto...
    Speaker: Roland Leathers, PhD , Jeroen de Vrij, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    41 1
    SEP 13, 2023 | 7:00 AM
    Developing genotyping platforms to enable discovery, quality control and genomic breeding at CoverCress Inc.
    Developing genotyping platforms to enable discovery, quality control...
    As the demand for renewal fuel feedstocks continues to grow, non-food feedstocks production must significantly increase to meet demand. Winter oilseeds grown in cooler climates provide oppor...
    Speaker: Ratan Chopra, PhD
    Sponsored By: LGC Biosearch Technologies
    17
    AUG 29, 2023 | 8:00 AM
    The Powers of Odin:  Comprehensive Cellular Characterization
    The Powers of Odin: Comprehensive Cellular Characterization
    Prokaryotic and eukaryotic cells change and adapt to their environments in ways that genomic characterization methods do not fully capture or predict. Even strains of the same species of mic...
    Speaker: Dina Finan, PhD
    Sponsored By: Biolog
    14
    JUL 26, 2023 | 4:05 PM
    Discovery and engineering of a miniature CRISPR-Cas type V-L system
    Discovery and engineering of a miniature CRISPR-Cas type V-L system
    Employing a metagenomic search, we identified a family of miniature CRISPR-Cas type V-L systems capable of RNA-guided dsDNA target cleavage without a tracrRNA. A bacterial depletion screen r...
    Speaker: Lauren Alfonse
    Presented at: GenScript 3rd Annual Gene and Cell Engineering Summit
    6
    MAR 01, 2023 | 8:00 AM
    Maximizing metastasis insights: Using a novel integrated workflow approach to achieve efficient CTC enrichment, enumeration and identification
    Maximizing metastasis insights: Using a novel integrated workflow...
    Date: March 01, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Characterizing metastasis remains key for understanding and eradicating cancer. The study of circulating tumor cells (CTC...
    Speaker: Adam Corner, Ph.D.
    Sponsored By: Bio-Rad Laboratories
    8
     
     
  • FEB 22, 2023 | 9:00 AM
    A scalable miniature circular GenCircleâ„¢ dsDNA production system for gene and cell therapy
    A scalable miniature circular GenCircleâ„¢ dsDNA production system...
    Date: February 22, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) Circular plasmid DNA has traditionally been used as a stable and efficient vector for gene expression and HDR-mediated...
    Speaker: Dr. Fan Zhou, PhD
    Sponsored By: GenScript
    7
    JAN 18, 2023 | 9:00 AM
    Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
    Elucidating the composition of CRISPR-Cas12f1 complexes using...
    Date: January 18, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) CRISPR-Cas systems are widely used in genome engineering applications. They contain RNA-guided ribonucleoprotein (RNP)...
    Speaker: Selgar Henkel-Heinecke
    Sponsored By: Refeyn
    13
    OCT 11, 2022 | 8:00 AM
    Smarter sample preparation for single-cell sequencing
    Smarter sample preparation for single-cell sequencing
    Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
    Speaker: Carina Emery
    Sponsored By: Miltenyi Biotec
    22
    OCT 04, 2022 | 9:00 AM
    Enabling fully automated NGS sample preparation using digital microfluidics
    Enabling fully automated NGS sample preparation using digital...
    Date: October 04, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) The Miro Canvas is a new platform which enables full walkaway automation of complex next generation sequencing (NGS) s...
    Speaker: Dr. Jonathan Levine , Dr. Fay Christodoulou
    Sponsored By: Miroculus
    13
    SEP 27, 2022 | 8:00 AM
    Efficient and Rapid Discovery of Novel Temperature Sensitive Alleles with the Onyx® Digital Genome Engineering Platform
    Efficient and Rapid Discovery of Novel Temperature Sensitive Alleles...
    Date: September 27, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Temperature sensitive (TS) alleles are mutations in essential genes that allow for growth at permissive temperatures...
    Speaker: Randy Lacey
    Sponsored By: Inscripta
    10 2
     
     
  • SEP 22, 2022 | 9:00 AM
    Human proteomics: from the operating room to the lab and back
    Human proteomics: from the operating room to the lab and back
    Date: September 22, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Optimizing platforms for surgical specimen collection and deep human phenotyping was used to enhance protein biomarke...
    Speaker: Vinit Mahajan M.D., Ph.D.
    Sponsored By: SomaLogic
    17
    SEP 15, 2022 | 8:00 AM
    Tools and partnerships for sustainably increasing rice production
    Tools and partnerships for sustainably increasing rice production
    Date: September 15, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Improving rice to withstand diseases, climate-related stresses and reduced inputs will contribute to food security a...
    Speaker: Dr. Katherine Steele , Darshna Vyas
    Sponsored By: LGC Biosearch Technologies
    10
    MAY 19, 2022 | 8:00 AM
    Automated sample preparation for reproducible clinical proteomics
    Automated sample preparation for reproducible clinical proteomics
    Date: May 19, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classific...
    Speaker: Jeroen Krijgsveld , Nicolas Autret, PhD , Ignasi Forné, PhD
    Sponsored By: Covaris, Inc.
    14
    APR 28, 2022 | 9:00 AM
    Beyond infection prevention and control: How genome sequencing of AMR pathogens can improve antibiotic stewardship and patient care
    Beyond infection prevention and control: How genome sequencing...
    Date: April 28, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CET) During previous webinars we have discussed the global impact of antimicrobial resistance (AMR) infections, explored biolo...
    Speaker: Dr. Arne Materna
    Sponsored By: OpGen
    11
    APR 26, 2022 | 7:00 AM
    High-Content Phenotypic Screening - Answering Questions You Haven't Even Asked Yet
    High-Content Phenotypic Screening - Answering Questions You Haven't...
    Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
    Speaker: Dr. Angeline Lim , Dr. Victor Wong
    Sponsored By: Molecular Devices
    5
     
     
  • APR 20, 2022 | 5:30 AM
    Improving Fetal Fraction of EDTA-Gel NIPS Samples Using Gel-Based Size-Selection
    Improving Fetal Fraction of EDTA-Gel NIPS Samples Using Gel-Based...
    Objective : Evaluation of EDTA-gel blood collection tubes from remote locations for NIPS (Non-invasive prenatal testing). Design: Paired comparisons Population: 61 pregnant women from Britis...
    Speaker: Dr François Rousseau, MD, MSc, FRCPC, FCAHS
    Presented at: Genetics Week Virtual Event Series 2022
    3
    APR 05, 2022 | 9:00 AM
    Enterovirus membrane-assisted assembly and release revealed by cryo-electron tomography
    Enterovirus membrane-assisted assembly and release revealed by...
    Date: April 5, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases...
    Speaker: Selma Dahmane , Kristian Wadel
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific - Electron Microscopy
    7
    DEC 09, 2021 | 11:00 AM
    Surveillance and AI-Powered Prediction of AMR to Inform Antibiotic Therapy
    Surveillance and AI-Powered Prediction of AMR to Inform Antibiotic...
    Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
    Speaker: Dr. Arne Materna
    Sponsored By: OpGen
    7
    DEC 08, 2021 | 9:00 AM
    The MAD7â„¢ nuclease in plant editing: overcoming CRISPR bottlenecks with MAD TiGER
    The MAD7â„¢ nuclease in plant editing: overcoming CRISPR bottlenecks...
    Date: December 9, 2021 Time: 9:00am (PDT), 12:00am (EDT) CRISPR editing has revolutionized plant breeding, and the MAD7 CRISPR nuclease developed by InscriptaTM is democratizing access to CR...
    Speaker: Gabino Sanchez , Ben Mijts
    Sponsored By: Inscripta
    5
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