Long-Read Sequencing, also known as third-generation sequencing, is a class of DNA sequencing methods which are currently under active development. In contrast to other existing methods of DNA sequencing the process of long-read sequencing does not require breaking long strands of DNA, but simply reading the nucleotide sequences at a molecule level.
The complete assembly of each human chromosome is essential for understanding human biology and evolution. Using complementary long-read sequencing technologies, we complete the first linear...
Recently, long-read sequencing with high accuracy has become a reality. Previous technologies allowed for the detection of particular classes of genetic variation and/or have focused on pre-...
The utilization of high fidelity long read sequencing is critical for the resolution of repetitive genomes. Cannabis sativa is known to be diploid (2n=20), 70% repetitive, 64% AT rich and 1%...
This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
Date: October 28, 2020 Time: 7:00am (PDT), 10:00am (EDT) Although chemotherapy remains the mainstay of systemic therapy, a large number of cancer patients fail to respond to it. About half o...
Changes in transcriptional regulation are thought to be one of the key drivers of carcinogenesis. Although next-generation sequencer revolutionized transcriptome analysis, there are limitati...
A repeat expansion in the C9orf72-SMCR8 complex subunit (C9orf72) represents the most common genetic cause of two fatal neurodegenerative diseases: frontotemporal dementia (FTD) and amyotrop...
An extensively debated concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-t...
Focusing on the urgent clinical problem of increasing carbapenem resistance in Enterobacteriaceae we have been evaluating detection methods in clinical microbiology and molecular transmissio...
The microbiome has emerged as a major contributor to human health and disease. Numerous sources implicate shifts in the gut microbiome as potentially pathologic for a variety of autoimmune d...
The transfer of antimicrobial resistance genes (ARG) to pathogenic microbes is a major concern in modern medicine. Antibiotic therapies are often rendered ineffective by horizontal acquisiti...
Human chromosome 19q13.4 contains genes encoding killer-cell immunoglobulin-like receptors (KIR). The region has certain properties such as single nucleotide variation, structural variation,...
The regions of our genome responsible for encoding the genes that regulate our immune response are some of the most complex and polymorphic known. This complexity encompasses multiple types...
The development of automated DNA sequencers using fluorescent di-deoxy nucleotide sequencing and capillary electrophoresis made it possible to generate the first draft sequences of the human...
Complex genomes, including the human genome, contain ‘dark’ regions that standard short-read sequencing technologies do not adequately resolve—overlooking many variants tha...
Within the clinical arena, the oncology community has led the way in the early adoption of next generation sequencing. NGS is in widespread use in clinical cancer research and is now beginni...
The introduction of PacBio HiFi sequence reads, which are both long (up to 25 kb currently) and accurate (>99%) at the individual single-molecule sequence read level, has allowed for adva...
Long-read DNA sequencing technologies such as the Pacific Biosciences (PacBio) and Oxford Nanopore (ONT) platforms, have demonstrated enhanced detection of genomic variation, including Singl...
Learning Objectives: 1. Learn why the complexity and repetitive structure of the human genome makes it impossible to detect all structural variants using short-read sequencing 2. Learn how h...
Structural variation within plant genomes has fueled domestication and selection of traits critical for modern breeding of crop plants. Nevertheless, dissecting the diversity of structural v...
Single cell RNA sequencing (scRNA-seq) emerged to characterize gene expression differences between individual cells, allowing a higher resolution look at mRNA abundance than bulk RNA- seq. H...
DATE: April 2, 2020 TIME: 8:00am PT, 11:00am ET Cyclins A2 and E1 regulate the cell cycle by promoting S phase entry and progression. We recently identified a hepatocellular carcinoma (HCC)...
DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
The complete assembly of each human chromosome is essential for understanding human biology and evolution. Using complementary long-read sequencing technologies, we complete the first linear...
Recently, long-read sequencing with high accuracy has become a reality. Previous technologies allowed for the detection of particular classes of genetic variation and/or have focused on pre-...
The utilization of high fidelity long read sequencing is critical for the resolution of repetitive genomes. Cannabis sativa is known to be diploid (2n=20), 70% repetitive, 64% AT rich and 1%...
This drug development program is designed to create a family of broad-spectrum, pan-coronaviral drugs that respectively inhibit multiple key enzymes required for viral replication. By target...
Date: October 28, 2020 Time: 7:00am (PDT), 10:00am (EDT) Although chemotherapy remains the mainstay of systemic therapy, a large number of cancer patients fail to respond to it. About half o...
Changes in transcriptional regulation are thought to be one of the key drivers of carcinogenesis. Although next-generation sequencer revolutionized transcriptome analysis, there are limitati...
A repeat expansion in the C9orf72-SMCR8 complex subunit (C9orf72) represents the most common genetic cause of two fatal neurodegenerative diseases: frontotemporal dementia (FTD) and amyotrop...
An extensively debated concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-t...
Focusing on the urgent clinical problem of increasing carbapenem resistance in Enterobacteriaceae we have been evaluating detection methods in clinical microbiology and molecular transmissio...
The microbiome has emerged as a major contributor to human health and disease. Numerous sources implicate shifts in the gut microbiome as potentially pathologic for a variety of autoimmune d...
The transfer of antimicrobial resistance genes (ARG) to pathogenic microbes is a major concern in modern medicine. Antibiotic therapies are often rendered ineffective by horizontal acquisiti...
Human chromosome 19q13.4 contains genes encoding killer-cell immunoglobulin-like receptors (KIR). The region has certain properties such as single nucleotide variation, structural variation,...
The regions of our genome responsible for encoding the genes that regulate our immune response are some of the most complex and polymorphic known. This complexity encompasses multiple types...
The development of automated DNA sequencers using fluorescent di-deoxy nucleotide sequencing and capillary electrophoresis made it possible to generate the first draft sequences of the human...
Complex genomes, including the human genome, contain ‘dark’ regions that standard short-read sequencing technologies do not adequately resolve—overlooking many variants tha...
Within the clinical arena, the oncology community has led the way in the early adoption of next generation sequencing. NGS is in widespread use in clinical cancer research and is now beginni...
The introduction of PacBio HiFi sequence reads, which are both long (up to 25 kb currently) and accurate (>99%) at the individual single-molecule sequence read level, has allowed for adva...
Long-read DNA sequencing technologies such as the Pacific Biosciences (PacBio) and Oxford Nanopore (ONT) platforms, have demonstrated enhanced detection of genomic variation, including Singl...
Learning Objectives: 1. Learn why the complexity and repetitive structure of the human genome makes it impossible to detect all structural variants using short-read sequencing 2. Learn how h...
Structural variation within plant genomes has fueled domestication and selection of traits critical for modern breeding of crop plants. Nevertheless, dissecting the diversity of structural v...
Single cell RNA sequencing (scRNA-seq) emerged to characterize gene expression differences between individual cells, allowing a higher resolution look at mRNA abundance than bulk RNA- seq. H...
DATE: April 2, 2020 TIME: 8:00am PT, 11:00am ET Cyclins A2 and E1 regulate the cell cycle by promoting S phase entry and progression. We recently identified a hepatocellular carcinoma (HCC)...
DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
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