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    Microscopy studies

    Microscopy is a broad category encompassing various techniques that use microscopes to visualize samples and specimens that can't be seen by the naked eye. Microscopy studies can use a range of microscope platforms including simple microscopes, compound microscopes, electron microscopes, stereomicroscopes, confocal microscopes, and Scanning probe microscopes.

    Webinars

    • AUG 25, 2021 | 9:00 AM
      Keeping up with the chemists - Cloud processing for pharma cryo-EM
      Keeping up with the chemists - Cloud processing for pharma cryo-EM
      Date: August 25, 2021 Time: 9:00am PDT During this interactive webinar you can ask questions, get answers, and engage with Taiana De Oliveira from AstraZeneca, Steven Litster, Brian Skjerven...
      Speaker: Taiana Maia de Oliveira , Steve Litster , Brian Skjerven , Natalie White , Ieva Drulyte, PhD
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      9
      JUL 27, 2021 | 9:00 AM
      Cryo-EM in discovery and rational engineering of biologics
      Cryo-EM in discovery and rational engineering of biologics
      Date: July 27, 2021 Time: 9:00am PDT Dr. Yuri Iozzo presents "An integrated structural biology platform specialized for sub-100 kDa protein complexes to support biologics discovery and...
      Speaker: Yuri Iozzo , Dan Cannon
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      5
      JUN 29, 2021 | 11:00 AM
      Advancing Cellular Ultrastructure Research with Freeze-Fracture and Freeze-Etching
      Advancing Cellular Ultrastructure Research with Freeze-Fracture...
      Date: June 29, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the...
      Speaker: Evan Krystofiak , Seth Villarreal
      Sponsored By: Leica Microsystems
      9 1
      JUN 24, 2021 | 9:00 AM
      Structural Oncology - Fighting Cancer with Cryo-EM
      Structural Oncology - Fighting Cancer with Cryo-EM
      Date: June 24, 2021 Time: 9:00am PDT Currently, there are insufficient therapies to fully eradicate human cancer or predict who it will strike. Rational-drug design based on structural insi...
      Speaker: Deb Kelly
      Sponsored By: Thermo Fisher Scientific
      5
      JUN 16, 2021 | 8:00 AM
      Simplicity that defies complexity - the MACSima™ Platform for ultrahigh-content imaging
      Simplicity that defies complexity - the MACSima™ Platform for...
      Date: June 16, 2021 Time: 8:00am PDT The MACSima™ Imaging Platform is the only complete solution for ultrahigh-content imaging of hundreds of markers, allowing you to understand the tu...
      Speaker: Dr. Ali Kinkhabwala , Dr. Markus Habich
      Sponsored By: Miltenyi Biotec
      7
      JUN 15, 2021 | 10:00 AM
      Single cell & label free binding affinity: applications with Genentech
      Single cell & label free binding affinity: applications with Genentech
      Date: June 15, 2021 Time: 10:00am (PDT), 1:00pm (EDT) SPRm (Surface Plasmon Resonance Microscopy) is a novel technology that enables label-free and real-time binding affinity and kinetics of...
      Speaker: Elaine Mai, MS , Dr. Shijie Wu
      Sponsored By: Biosensing Instrument
      3
      JUN 10, 2021 | 8:00 AM
      Capture Life As It Happens - The New Leica Nano Workflow
      Capture Life As It Happens - The New Leica Nano Workflow
      Date: June 10, 2021 Time: 8:00am (PDT), 11:00am (EDT) With the new Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light a...
      Speaker: Dr. Julia Koenig
      Sponsored By: Leica Microsystems
      3
      JUN 09, 2021 | 7:00 AM
      Preventing cell misidentification in your daily lab routine
      Preventing cell misidentification in your daily lab routine
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      Speaker: Gabriela Saldanha , Jessica Wagener, PhD
      Sponsored By: Eppendorf
      40 3
      JUN 02, 2021 | 9:00 AM
      Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
      Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
      Over the past 25 years, Luminex’s xMAP® Technology has emerged as the worldwide leader in bead-based multiplexing. The new xMAP® INTELLIFLEX platform combines the proven perfor...
      Speaker: Katie Gillis , Brian E. Hall , Stephanie Brunelle, Ph.D.
      Presented at: Immuno-Oncology Virtual Event Series 2021
      Sponsored By: Luminex - A DiaSorin Company
      1
      APR 29, 2021 | 9:00 AM
      Cryo-Electron Microscopy: membrane proteins down to atoms
      Cryo-Electron Microscopy: membrane proteins down to atoms
      Date: April 29, 2021 Time: 9:00am PST Cryo-electron microscopy (cryo-EM) reached single-atom resolution for 3D structure determination of biological macromolecules. Cryo-EM has been determin...
      Speaker: Stephen Brohawn, PhD , Abhay Kotecha, PhD , Marc Storms
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      8
      APR 27, 2021 | 7:00 AM
      High Resolution Imaging of Zebrafish at Nano and Millimeter Scale
      High Resolution Imaging of Zebrafish at Nano and Millimeter Scale
      Date: April 27, 2021 Time: 7:00am (PDT), 11:00am (EDT) Visualization is an important step to address complex biological questions in vivo. The imaging process can widen the perspective of an...
      Speaker: Julien Resseguier , Claudia Florindo, PhD
      Sponsored By: Andor
      8
      APR 13, 2021 | 3:00 PM
      A physiologically-relevant 3D ECM for in vitro oncology research and intelligent high-content imaging of 3D models
      A physiologically-relevant 3D ECM for in vitro oncology research...
      Date: April 13, 2021 Time: 07:00am PST Trends continue towards the development of more sophisticated and physiologically relevant 3D in vitro models, such as stem cell-derived models, organo...
      Speaker: Elodie Vandenhaute , Guillaume Frugier
      Sponsored By: Molecular Devices
      4
      MAR 31, 2021 | 9:00 AM
      Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored targeting
      Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored...
      Date: March 31, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Knockdown studies are prevalent and powerful ways to illustrate gene function. CRISPRi delivers a new mechanism for gene knockdown, PAM...
      Speaker: Steve Smith, PhD, MBA
      Sponsored By: Horizon Discovery
      13 2
      MAR 25, 2021 | 8:00 AM
      Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles
      Expanding ERF (Equivalent Number of Reference Fluorophore) Value...
      Date: March 25, 2021 Time: 8:00am (PST), 11:00am (EST) Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data...
      Speaker: Paul DeRose
      Sponsored By: Thermo Fisher Scientific
      12
      MAR 09, 2021 | 8:00 AM
      Ultrahigh-content imaging: a breath of fresh air for lung cancer research
      Ultrahigh-content imaging: a breath of fresh air for lung cancer...
      Date: March 09, 2021 Time: 8:00am (PST), 11:00am (EST) Lung cancer is one of the three most common types of cancer. Representing 85% of all lung cancers...
      Speaker: Dr. Telma Santos
      Sponsored By: Miltenyi Biotec
      13
      FEB 24, 2021 | 10:30 AM
      The Visiopharm Platform: Flexible tools to solve real-world problems
      The Visiopharm Platform: Flexible tools to solve real-world problems
      Image analysis in a real-world situation rarely involves solving a single unchanging problem. Modern tools need to be flexible and adaptable not only to solve todays problems but to support...
      Speaker: Keith Bowers, PhD , Dave Mason, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      Sponsored By: Visiopharm®
      1
      FEB 03, 2021 | 8:00 AM
      An introduction to immuno-oncology at Miltenyi Biotec
      An introduction to immuno-oncology at Miltenyi Biotec
      Date: February 3, 2021 Time: 8:00am (PST), 11:00am (EST) This introduction webinar will provide you with an overview of the general immuno-oncology (IO) research field...
      Speaker: Dr. Marcello Stein
      Sponsored By: Miltenyi Biotec
      19 2
      DEC 17, 2020 | 9:00 AM
      Advancing Cell Biology with Cryo-Correlative Microscopy
      Advancing Cell Biology with Cryo-Correlative Microscopy
      Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
      Speaker: Elizabeth Wright, Ph.D. , Jae Yang, Ph.D. , Bryan Sibert, Ph.D. , Joseph Kim
      Sponsored By: Leica Microsystems, Alveole
      13 2
      DEC 09, 2020 | 8:00 AM
      Nanoscopy Meets Lifetime - Introducing the new STELLARIS 8 τ-STED
      Nanoscopy Meets Lifetime - Introducing the new STELLARIS 8 τ-STED
      Date: December 89 2020 Time: 8:00am (PST), 11:00am (EST) This talk introduces the Leica STELLARIS 8 τ-STED system where STED Nanoscopy meets Leica’s Fast Lifetime Contrast (FALCON)...
      Speaker: Julia Roberti, Ph.D.
      Sponsored By: Leica Microsystems
      1
      DEC 02, 2020 | 8:00 AM
      Optimize your bioprocess - From shaker to bioreactor
      Optimize your bioprocess - From shaker to bioreactor
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      Speaker: Ines Hartmann , David Solbach
      Sponsored By: Eppendorf
      11
      NOV 19, 2020 | 9:00 AM
      Highly specific multiplexed RNA imaging in tissues with split-FISH
      Highly specific multiplexed RNA imaging in tissues with split-FISH
      Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
      Speaker: Kok Hao Chen , Alan Mullan, PhD
      Sponsored By: Andor
      6 2
      NOV 18, 2020 | 8:00 AM
      Whole-organ mapping using AI and light sheet microscopy
      Whole-organ mapping using AI and light sheet microscopy
      DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
      Speaker: Ali Ertürk
      Sponsored By: Miltenyi Biotec
      14
      OCT 26, 2020 | 11:00 AM
      Introduction to FLIM-FRET techniques
      Introduction to FLIM-FRET techniques
      This session introduces basic concepts of imaging-based high-throughput screening (HTS) and high-throughput profiling assay development. Imaging-based HTS assays are designed to evaluate a d...
      Speaker: David Andrews, PhD
      Presented at: 7th Annual Conference: SBI2 High Content
      1
      OCT 21, 2020 | 10:30 AM
      Introduction to Supel(TM) Swift HLB: Sample Prep and Applications
      Introduction to Supel(TM) Swift HLB: Sample Prep and Applications
      Solid phase extraction has become a preferable technique for reducing matrix effects for sample preparation due to its ease of use and limited time requirements. MilliporeSigma has launched...
      Speaker: M. James Ross, PhD
      Presented at: Analytical Chemistry & Separation Sciences Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      3
    AUG 25, 2021 | 9:00 AM
    Keeping up with the chemists - Cloud processing for pharma cryo-EM
    Keeping up with the chemists - Cloud processing for pharma cryo-EM
    Date: August 25, 2021 Time: 9:00am PDT During this interactive webinar you can ask questions, get answers, and engage with Taiana De Oliveira from AstraZeneca, Steven Litster, Brian Skjerven...
    Speaker: Taiana Maia de Oliveira , Steve Litster , Brian Skjerven , Natalie White , Ieva Drulyte, PhD
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    9
    JUL 27, 2021 | 9:00 AM
    Cryo-EM in discovery and rational engineering of biologics
    Cryo-EM in discovery and rational engineering of biologics
    Date: July 27, 2021 Time: 9:00am PDT Dr. Yuri Iozzo presents "An integrated structural biology platform specialized for sub-100 kDa protein complexes to support biologics discovery and...
    Speaker: Yuri Iozzo , Dan Cannon
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    5
    JUN 29, 2021 | 11:00 AM
    Advancing Cellular Ultrastructure Research with Freeze-Fracture and Freeze-Etching
    Advancing Cellular Ultrastructure Research with Freeze-Fracture...
    Date: June 29, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Freeze-fracture and freeze-etching are useful tools for studying flexible membrane-associated structures such as tight junctions or the...
    Speaker: Evan Krystofiak , Seth Villarreal
    Sponsored By: Leica Microsystems
    9 1
    JUN 24, 2021 | 9:00 AM
    Structural Oncology - Fighting Cancer with Cryo-EM
    Structural Oncology - Fighting Cancer with Cryo-EM
    Date: June 24, 2021 Time: 9:00am PDT Currently, there are insufficient therapies to fully eradicate human cancer or predict who it will strike. Rational-drug design based on structural insi...
    Speaker: Deb Kelly
    Sponsored By: Thermo Fisher Scientific
    5
    JUN 16, 2021 | 8:00 AM
    Simplicity that defies complexity - the MACSima™ Platform for ultrahigh-content imaging
    Simplicity that defies complexity - the MACSima™ Platform for...
    Date: June 16, 2021 Time: 8:00am PDT The MACSima™ Imaging Platform is the only complete solution for ultrahigh-content imaging of hundreds of markers, allowing you to understand the tu...
    Speaker: Dr. Ali Kinkhabwala , Dr. Markus Habich
    Sponsored By: Miltenyi Biotec
    7
     
     
  • JUN 15, 2021 | 10:00 AM
    Single cell & label free binding affinity: applications with Genentech
    Single cell & label free binding affinity: applications with Genentech
    Date: June 15, 2021 Time: 10:00am (PDT), 1:00pm (EDT) SPRm (Surface Plasmon Resonance Microscopy) is a novel technology that enables label-free and real-time binding affinity and kinetics of...
    Speaker: Elaine Mai, MS , Dr. Shijie Wu
    Sponsored By: Biosensing Instrument
    3
    JUN 10, 2021 | 8:00 AM
    Capture Life As It Happens - The New Leica Nano Workflow
    Capture Life As It Happens - The New Leica Nano Workflow
    Date: June 10, 2021 Time: 8:00am (PDT), 11:00am (EDT) With the new Leica Nano Workflow, searching for the needle in the haystack is a thing of the past. Take advantage of correlative light a...
    Speaker: Dr. Julia Koenig
    Sponsored By: Leica Microsystems
    3
    JUN 09, 2021 | 7:00 AM
    Preventing cell misidentification in your daily lab routine
    Preventing cell misidentification in your daily lab routine
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    Speaker: Gabriela Saldanha , Jessica Wagener, PhD
    Sponsored By: Eppendorf
    40 3
    JUN 02, 2021 | 9:00 AM
    Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
    Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
    Over the past 25 years, Luminex’s xMAP® Technology has emerged as the worldwide leader in bead-based multiplexing. The new xMAP® INTELLIFLEX platform combines the proven perfor...
    Speaker: Katie Gillis , Brian E. Hall , Stephanie Brunelle, Ph.D.
    Presented at: Immuno-Oncology Virtual Event Series 2021
    Sponsored By: Luminex - A DiaSorin Company
    1
    APR 29, 2021 | 9:00 AM
    Cryo-Electron Microscopy: membrane proteins down to atoms
    Cryo-Electron Microscopy: membrane proteins down to atoms
    Date: April 29, 2021 Time: 9:00am PST Cryo-electron microscopy (cryo-EM) reached single-atom resolution for 3D structure determination of biological macromolecules. Cryo-EM has been determin...
    Speaker: Stephen Brohawn, PhD , Abhay Kotecha, PhD , Marc Storms
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    8
     
     
  • APR 27, 2021 | 7:00 AM
    High Resolution Imaging of Zebrafish at Nano and Millimeter Scale
    High Resolution Imaging of Zebrafish at Nano and Millimeter Scale
    Date: April 27, 2021 Time: 7:00am (PDT), 11:00am (EDT) Visualization is an important step to address complex biological questions in vivo. The imaging process can widen the perspective of an...
    Speaker: Julien Resseguier , Claudia Florindo, PhD
    Sponsored By: Andor
    8
    APR 13, 2021 | 3:00 PM
    A physiologically-relevant 3D ECM for in vitro oncology research and intelligent high-content imaging of 3D models
    A physiologically-relevant 3D ECM for in vitro oncology research...
    Date: April 13, 2021 Time: 07:00am PST Trends continue towards the development of more sophisticated and physiologically relevant 3D in vitro models, such as stem cell-derived models, organo...
    Speaker: Elodie Vandenhaute , Guillaume Frugier
    Sponsored By: Molecular Devices
    4
    MAR 31, 2021 | 9:00 AM
    Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored targeting
    Gene inactivation by CRISPRi: Modulate gene expression with PAM-anchored...
    Date: March 31, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Knockdown studies are prevalent and powerful ways to illustrate gene function. CRISPRi delivers a new mechanism for gene knockdown, PAM...
    Speaker: Steve Smith, PhD, MBA
    Sponsored By: Horizon Discovery
    13 2
    MAR 25, 2021 | 8:00 AM
    Expanding ERF (Equivalent Number of Reference Fluorophore) Value Assignments for Quantitative Flow Cytometry- More Colors and Smaller Particles
    Expanding ERF (Equivalent Number of Reference Fluorophore) Value...
    Date: March 25, 2021 Time: 8:00am (PST), 11:00am (EST) Flow cytometry has expanded to have more users, more data, and multiple instrument types. While this has lead to more quantitative data...
    Speaker: Paul DeRose
    Sponsored By: Thermo Fisher Scientific
    12
    MAR 09, 2021 | 8:00 AM
    Ultrahigh-content imaging: a breath of fresh air for lung cancer research
    Ultrahigh-content imaging: a breath of fresh air for lung cancer...
    Date: March 09, 2021 Time: 8:00am (PST), 11:00am (EST) Lung cancer is one of the three most common types of cancer. Representing 85% of all lung cancers...
    Speaker: Dr. Telma Santos
    Sponsored By: Miltenyi Biotec
    13
     
     
  • FEB 24, 2021 | 10:30 AM
    The Visiopharm Platform: Flexible tools to solve real-world problems
    The Visiopharm Platform: Flexible tools to solve real-world problems
    Image analysis in a real-world situation rarely involves solving a single unchanging problem. Modern tools need to be flexible and adaptable not only to solve todays problems but to support...
    Speaker: Keith Bowers, PhD , Dave Mason, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    Sponsored By: Visiopharm®
    1
    FEB 03, 2021 | 8:00 AM
    An introduction to immuno-oncology at Miltenyi Biotec
    An introduction to immuno-oncology at Miltenyi Biotec
    Date: February 3, 2021 Time: 8:00am (PST), 11:00am (EST) This introduction webinar will provide you with an overview of the general immuno-oncology (IO) research field...
    Speaker: Dr. Marcello Stein
    Sponsored By: Miltenyi Biotec
    19 2
    DEC 17, 2020 | 9:00 AM
    Advancing Cell Biology with Cryo-Correlative Microscopy
    Advancing Cell Biology with Cryo-Correlative Microscopy
    Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
    Speaker: Elizabeth Wright, Ph.D. , Jae Yang, Ph.D. , Bryan Sibert, Ph.D. , Joseph Kim
    Sponsored By: Leica Microsystems, Alveole
    13 2
    DEC 09, 2020 | 8:00 AM
    Nanoscopy Meets Lifetime - Introducing the new STELLARIS 8 τ-STED
    Nanoscopy Meets Lifetime - Introducing the new STELLARIS 8 τ-STED
    Date: December 89 2020 Time: 8:00am (PST), 11:00am (EST) This talk introduces the Leica STELLARIS 8 τ-STED system where STED Nanoscopy meets Leica’s Fast Lifetime Contrast (FALCON)...
    Speaker: Julia Roberti, Ph.D.
    Sponsored By: Leica Microsystems
    1
    DEC 02, 2020 | 8:00 AM
    Optimize your bioprocess - From shaker to bioreactor
    Optimize your bioprocess - From shaker to bioreactor
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    Speaker: Ines Hartmann , David Solbach
    Sponsored By: Eppendorf
    11
     
     
  • NOV 19, 2020 | 9:00 AM
    Highly specific multiplexed RNA imaging in tissues with split-FISH
    Highly specific multiplexed RNA imaging in tissues with split-FISH
    Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
    Speaker: Kok Hao Chen , Alan Mullan, PhD
    Sponsored By: Andor
    6 2
    NOV 18, 2020 | 8:00 AM
    Whole-organ mapping using AI and light sheet microscopy
    Whole-organ mapping using AI and light sheet microscopy
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    Speaker: Ali Ertürk
    Sponsored By: Miltenyi Biotec
    14
    OCT 26, 2020 | 11:00 AM
    Introduction to FLIM-FRET techniques
    Introduction to FLIM-FRET techniques
    This session introduces basic concepts of imaging-based high-throughput screening (HTS) and high-throughput profiling assay development. Imaging-based HTS assays are designed to evaluate a d...
    Speaker: David Andrews, PhD
    Presented at: 7th Annual Conference: SBI2 High Content
    1
    OCT 21, 2020 | 10:30 AM
    Introduction to Supel(TM) Swift HLB: Sample Prep and Applications
    Introduction to Supel(TM) Swift HLB: Sample Prep and Applications
    Solid phase extraction has become a preferable technique for reducing matrix effects for sample preparation due to its ease of use and limited time requirements. MilliporeSigma has launched...
    Speaker: M. James Ross, PhD
    Presented at: Analytical Chemistry & Separation Sciences Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    3
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