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    Plasmid

    A plasmid is a small circular piece of DNA, wholly separate from the genomic DNA, most commonly found in Bacteria and Archaea. Often encoding event-restricted genes that aid in organism survival, they can be divested if need be. Plasmids are often used in the biotech industry in order to clone recombinant proteins for expression by E. coli.

    Webinars

    • MAR 15, 2018 | 10:30 AM
      An Emerging Role of DNA Damage-Repair in Huntington's Disease
      An Emerging Role of DNA Damage-Repair in Huntington's Disease
      Machine learning approaches to pattern discovery in protein-protein interaction networks of Huntington’s disease brain and model organisms are revealing novel connections of the disease...
      Speaker: Animesh Ray, PhD
      Presented at: Neuroscience Virtual Event Series 2018
      2
      FEB 22, 2018 | 10:30 AM
      Illuminating the Druggable GPCR-ome
      Illuminating the Druggable GPCR-ome
      G-protein coupled receptors (GPCRs) represent the single largest class of druggable targets in the human genome. Of the 390 or so druggable and non-olfactory human GPCRs there exist many whic...
      Speaker: Bryan L. Roth, MD, PhD
      Presented at: Drug Discovery Virtual Event Series 2018
      1
      NOV 14, 2017 | 9:00 AM
      WEBINAR: CRISPRa tools for transcriptional activation studies
      WEBINAR: CRISPRa tools for transcriptional activation studies
      DATE: November 14, 2017TIME: 09:00am PST, 12:00pm ESTThe CRISPR-Cas9 system has been adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and av...
      Speaker: Žaklina Strezoska , Benedict Cross
      Sponsored By: Dharmacon, a Horizon Discovery Group Company
      11 4
      AUG 22, 2017 | 9:00 AM
      WEBINAR: A virtual workshop for precise HDR-mediated genome engineering with CRISPR-Cas9
      WEBINAR: A virtual workshop for precise HDR-mediated genome engineering...
      DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
      Speaker: Maren Mayer Gross
      Sponsored By: Dharmacon
      12 5
      JUL 01, 2017 | 12:00 AM
      New Tools for improved CRISPR gene-editing in stem cells
      New Tools for improved CRISPR gene-editing in stem cells
      The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
      Speaker: James Kehler VMD, PhD
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      Highly efficient stem cell engineering via Cas9 protein transfection
      Highly efficient stem cell engineering via Cas9 protein transfection
      CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering.  The delivery of Cas9 plasmid DNA or mR...
      Speaker: Xiquan Liang
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      2
      JUN 28, 2017 | 7:00 AM
      Analytical methods to measure empty and full AAV particles
      Analytical methods to measure empty and full AAV particles
      DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
      Speaker: Christine LeBec, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences
      9 2
      MAY 10, 2017 | 4:00 PM
      Ensuring successful protein expression through proper plasmid purification
      Ensuring successful protein expression through proper plasmid...
      Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
      Speaker: Sasha Vlassov, PhD
      Presented at: 2017 Gibco ExpressionWorld
      3
      MAY 10, 2017 | 3:00 PM
      Large scale serum free suspension lentiviral production for cell and gene therapy application
      Large scale serum free suspension lentiviral production for cell...
      Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
      Speaker: Yongchang Ji, PhD.
      Presented at: 2017 Gibco ExpressionWorld
      4 1
      MAY 10, 2017 | 8:00 AM
      Optimisation of UCB's Transient Expression Platforms
      Optimisation of UCB's Transient Expression Platforms
      Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
      Speaker: Christina Gordon
      Presented at: 2017 Gibco ExpressionWorld
      4
      APR 05, 2017 | 7:15 AM
      Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
      Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
      CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
      Speaker: John Schiel, Ph.D.
      Presented at: Protein Research Live
      5
      APR 05, 2017 | 6:00 AM
      A workflow for CRISPR-Cas9 high throughput arrayed screening with synthetic crRNA
      A workflow for CRISPR-Cas9 high throughput arrayed screening with...
      There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
      Speaker: Louise Baskin
      Presented at: Protein Research Live
      20
      FEB 20, 2017 | 8:00 AM
      Presenting a complete workflow - from start to finish!
      Presenting a complete workflow - from start to finish!
      The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
      Speaker: Emily Anderson, Ph.D.
      Presented at: Protein Research Live
      4
      FEB 20, 2017 | 8:00 AM
      Getting started with siRNA: considerations and recommendations
      Getting started with siRNA: considerations and recommendations
      This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...
      Speaker: Louise Baskin
      Presented at: Protein Research Live
      3
      NOV 17, 2016 | 7:00 AM
      From heart to mind: Simplified iPS cell reprogramming into cardiac and neural cells under hypoxic conditions
      From heart to mind: Simplified iPS cell reprogramming into cardiac...
      DATE: November 17, 2016 TIME: 7:00am PT, 10:00am ET Induced pluripotent stem (iPS) cell reprogramming allows turning an adult somatic cell into a pluripotent stem cell. Four Fac...
      Speaker: Rick Cohen, Ph.D
      Sponsored By: Eppendorf, Eppendorf
      5
      JUN 23, 2016 | 8:00 AM
      A workflow for CRISPR-Cas9 high throughput arrayed screening with synthetic crRNA
      A workflow for CRISPR-Cas9 high throughput arrayed screening with...
      DATE:  June 23, 2016 TIME:  8am Pacific time, 11am Eastern time In this webinar you will learn: Key factors in successful arrayed crRNA screening Benefits of the dual CR...
      Speaker: Louise Baskin
      Sponsored By: Dharmacon, Dharmacon
      12
      JUN 09, 2016 | 9:00 AM
      Standardizing Microbiomics
      Standardizing Microbiomics
      DATE:  June 9, 2016 TIME:   9AM Pacific time, 12pm Eastern time The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and...
      Speaker: Ryan Kemp, M.S. , Shuiquan Tang, PhD
      Sponsored By: Zymo Research, Zymo Research
      13
      MAY 04, 2016 | 5:00 AM
      Complete workflow solution from gene-to-protein
      Complete workflow solution from gene-to-protein
      Reliability from gene sequence to the expressed and purified protein is essential for many aspects of basic research and drug development. Thermo Fisher Scientific’s complete portfolio ...
      Speaker: Michael Spring
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAR 31, 2016 | 8:00 AM
      Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
      Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
      DATE:  March 31, 2016 TIME:   8am PT, 11am ET, 4pm GMT In this webinar, you will learn:  Basics of homology-directed repair (HDR) using CRISPR-Cas9 Selection of CRISP...
      Speaker: John Schiel, Ph.D.
      Sponsored By: Dharmacon, Dharmacon
      31 12
      SEP 29, 2015 | 8:00 AM
      Improve CRISPR-Cas9 experiments with rationally designed guide RNAs
      Improve CRISPR-Cas9 experiments with rationally designed guide...
      To learn more about Gene Editing watch “Webinar: CRISPR-Cas9 Gene Editing with Synthetic RNA – from start to finish!”  Attendees will learn: the importance of ...
      Speaker: Louise Baskin
      Sponsored By: Dharmacon, Dharmacon
      11 4
      MAY 12, 2015 | 8:00 AM
      RNAi success in difficult-to-transfect cells by self-delivering siRNA
      RNAi success in difficult-to-transfect cells by self-delivering...
      Please click here to watch this webinar On DemandsiRNA-mediated silencing of gene expression has revolutionized the study of biology by enabling rapid and unbiased loss-of-function studies to...
      Speaker: Louise Baskin , Michael Freeley
      Sponsored By: Dharmacon, Dharmacon
      9 13
      FEB 19, 2015 | 8:00 AM
      Presenting a complete workflow - from start to finish!
      Presenting a complete workflow - from start to finish!
      For more on Gene Editing watch “Webinar: Improve CRISPR-Cas9 experiments with rationally designed guide RNAs” The CRISPR-Cas9 system is being widely used for genome engineering...
      Speaker: Emily Anderson, Ph.D.
      Sponsored By: Dharmacon, Dharmacon
      34 1
    MAR 15, 2018 | 10:30 AM
    An Emerging Role of DNA Damage-Repair in Huntington's Disease
    An Emerging Role of DNA Damage-Repair in Huntington's Disease
    Machine learning approaches to pattern discovery in protein-protein interaction networks of Huntington’s disease brain and model organisms are revealing novel connections of the disease...
    Speaker: Animesh Ray, PhD
    Presented at: Neuroscience Virtual Event Series 2018
    2
    FEB 22, 2018 | 10:30 AM
    Illuminating the Druggable GPCR-ome
    Illuminating the Druggable GPCR-ome
    G-protein coupled receptors (GPCRs) represent the single largest class of druggable targets in the human genome. Of the 390 or so druggable and non-olfactory human GPCRs there exist many whic...
    Speaker: Bryan L. Roth, MD, PhD
    Presented at: Drug Discovery Virtual Event Series 2018
    1
    NOV 14, 2017 | 9:00 AM
    WEBINAR: CRISPRa tools for transcriptional activation studies
    WEBINAR: CRISPRa tools for transcriptional activation studies
    DATE: November 14, 2017TIME: 09:00am PST, 12:00pm ESTThe CRISPR-Cas9 system has been adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and av...
    Speaker: Žaklina Strezoska , Benedict Cross
    Sponsored By: Dharmacon, a Horizon Discovery Group Company
    11 4
    AUG 22, 2017 | 9:00 AM
    WEBINAR: A virtual workshop for precise HDR-mediated genome engineering with CRISPR-Cas9
    WEBINAR: A virtual workshop for precise HDR-mediated genome engineering...
    DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
    Speaker: Maren Mayer Gross
    Sponsored By: Dharmacon
    12 5
    JUL 01, 2017 | 12:00 AM
    New Tools for improved CRISPR gene-editing in stem cells
    New Tools for improved CRISPR gene-editing in stem cells
    The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
    Speaker: James Kehler VMD, PhD
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    2
     
     
  • JUL 01, 2017 | 12:00 AM
    Highly efficient stem cell engineering via Cas9 protein transfection
    Highly efficient stem cell engineering via Cas9 protein transfection
    CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering.  The delivery of Cas9 plasmid DNA or mR...
    Speaker: Xiquan Liang
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    2
    JUN 28, 2017 | 7:00 AM
    Analytical methods to measure empty and full AAV particles
    Analytical methods to measure empty and full AAV particles
    DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
    Speaker: Christine LeBec, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences
    9 2
    MAY 10, 2017 | 4:00 PM
    Ensuring successful protein expression through proper plasmid purification
    Ensuring successful protein expression through proper plasmid...
    Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
    Speaker: Sasha Vlassov, PhD
    Presented at: 2017 Gibco ExpressionWorld
    3
    MAY 10, 2017 | 3:00 PM
    Large scale serum free suspension lentiviral production for cell and gene therapy application
    Large scale serum free suspension lentiviral production for cell...
    Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
    Speaker: Yongchang Ji, PhD.
    Presented at: 2017 Gibco ExpressionWorld
    4 1
    MAY 10, 2017 | 8:00 AM
    Optimisation of UCB's Transient Expression Platforms
    Optimisation of UCB's Transient Expression Platforms
    Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
    Speaker: Christina Gordon
    Presented at: 2017 Gibco ExpressionWorld
    4
     
     
  • APR 05, 2017 | 7:15 AM
    Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
    Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
    CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
    Speaker: John Schiel, Ph.D.
    Presented at: Protein Research Live
    5
    APR 05, 2017 | 6:00 AM
    A workflow for CRISPR-Cas9 high throughput arrayed screening with synthetic crRNA
    A workflow for CRISPR-Cas9 high throughput arrayed screening with...
    There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
    Speaker: Louise Baskin
    Presented at: Protein Research Live
    20
    FEB 20, 2017 | 8:00 AM
    Presenting a complete workflow - from start to finish!
    Presenting a complete workflow - from start to finish!
    The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
    Speaker: Emily Anderson, Ph.D.
    Presented at: Protein Research Live
    4
    FEB 20, 2017 | 8:00 AM
    Getting started with siRNA: considerations and recommendations
    Getting started with siRNA: considerations and recommendations
    This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...
    Speaker: Louise Baskin
    Presented at: Protein Research Live
    3
    NOV 17, 2016 | 7:00 AM
    From heart to mind: Simplified iPS cell reprogramming into cardiac and neural cells under hypoxic conditions
    From heart to mind: Simplified iPS cell reprogramming into cardiac...
    DATE: November 17, 2016 TIME: 7:00am PT, 10:00am ET Induced pluripotent stem (iPS) cell reprogramming allows turning an adult somatic cell into a pluripotent stem cell. Four Fac...
    Speaker: Rick Cohen, Ph.D
    Sponsored By: Eppendorf, Eppendorf
    5
     
     
  • JUN 23, 2016 | 8:00 AM
    A workflow for CRISPR-Cas9 high throughput arrayed screening with synthetic crRNA
    A workflow for CRISPR-Cas9 high throughput arrayed screening with...
    DATE:  June 23, 2016 TIME:  8am Pacific time, 11am Eastern time In this webinar you will learn: Key factors in successful arrayed crRNA screening Benefits of the dual CR...
    Speaker: Louise Baskin
    Sponsored By: Dharmacon, Dharmacon
    12
    JUN 09, 2016 | 9:00 AM
    Standardizing Microbiomics
    Standardizing Microbiomics
    DATE:  June 9, 2016 TIME:   9AM Pacific time, 12pm Eastern time The rapid growth of Microbiomics has increased the demand for standard methods to improve the reproducibility and...
    Speaker: Ryan Kemp, M.S. , Shuiquan Tang, PhD
    Sponsored By: Zymo Research, Zymo Research
    13
    MAY 04, 2016 | 5:00 AM
    Complete workflow solution from gene-to-protein
    Complete workflow solution from gene-to-protein
    Reliability from gene sequence to the expressed and purified protein is essential for many aspects of basic research and drug development. Thermo Fisher Scientific’s complete portfolio ...
    Speaker: Michael Spring
    Presented at: 2016 Gibco ExpressionWorld
    2
    MAR 31, 2016 | 8:00 AM
    Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
    Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
    DATE:  March 31, 2016 TIME:   8am PT, 11am ET, 4pm GMT In this webinar, you will learn:  Basics of homology-directed repair (HDR) using CRISPR-Cas9 Selection of CRISP...
    Speaker: John Schiel, Ph.D.
    Sponsored By: Dharmacon, Dharmacon
    31 12
    SEP 29, 2015 | 8:00 AM
    Improve CRISPR-Cas9 experiments with rationally designed guide RNAs
    Improve CRISPR-Cas9 experiments with rationally designed guide...
    To learn more about Gene Editing watch “Webinar: CRISPR-Cas9 Gene Editing with Synthetic RNA – from start to finish!”  Attendees will learn: the importance of ...
    Speaker: Louise Baskin
    Sponsored By: Dharmacon, Dharmacon
    11 4
     
     
  • MAY 12, 2015 | 8:00 AM
    RNAi success in difficult-to-transfect cells by self-delivering siRNA
    RNAi success in difficult-to-transfect cells by self-delivering...
    Please click here to watch this webinar On DemandsiRNA-mediated silencing of gene expression has revolutionized the study of biology by enabling rapid and unbiased loss-of-function studies to...
    Speaker: Louise Baskin , Michael Freeley
    Sponsored By: Dharmacon, Dharmacon
    9 13
    FEB 19, 2015 | 8:00 AM
    Presenting a complete workflow - from start to finish!
    Presenting a complete workflow - from start to finish!
    For more on Gene Editing watch “Webinar: Improve CRISPR-Cas9 experiments with rationally designed guide RNAs” The CRISPR-Cas9 system is being widely used for genome engineering...
    Speaker: Emily Anderson, Ph.D.
    Sponsored By: Dharmacon, Dharmacon
    34 1
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