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    Plasmid

    A plasmid is a small circular piece of DNA, wholly separate from the genomic DNA, most commonly found in Bacteria and Archaea. Often encoding event-restricted genes that aid in organism survival, they can be divested if need be. Plasmids are often used in the biotech industry in order to clone recombinant proteins for expression by E. coli.

    Webinars

    • NOV 19, 2019 | 8:00 AM
      Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows
      Overcoming the Hurdles of Density Gradient Ultracentrifugation...
      DATE: November 19, 2019TIME: 8:00am PTCentrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of discipline...
      Speaker: Ross VerHeul
      Sponsored By: Beckman Coulter Life Sciences
      7 1
      NOV 05, 2019 | 9:00 AM
      Minicircle DNA: a new material for CAR-T and AAV vector particles - its development and analysis
      Minicircle DNA: a new material for CAR-T and AAV vector particles...
      DATE: November 5, 2019TIME: 9:00am PT, 12:00pm ET New results have highlighted the advantages of minicircles over plasmids as they avoid unnecessary bacterial backbone sequences...
      Speaker: Martin Schleef, PhD
      Sponsored By: SCIEX
      4
      OCT 10, 2019 | 6:00 AM
      Highly-efficient and -specific genome editing with Cas9 nickase ribonucleoproteins in Primary T-cells
      Highly-efficient and -specific genome editing with Cas9 nickase...
      In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases wh...
      Speaker: Jacob Lamberth
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2019
      Sponsored By: MilliporeSigma
      1
      SEP 12, 2019 | 10:30 AM
      Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
      Analysis of human gut microbiomes and antibiotic resistomes of...
      The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
      Speaker: Jonathan Jacobs, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2019
      Sponsored By: QIAGEN
      5
      SEP 12, 2019 | 10:00 AM
      High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS
      High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV)...
      DATE: September 12, 2019TIME: 10:00am PDT, 1:00pm EDT...
      Speaker: Tingting Li, MS
      Sponsored By: SCIEX
      2
      JAN 31, 2019 | 7:30 AM
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
      Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
      Speaker: A.C. Matin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      1
      OCT 03, 2018 | 12:00 PM
      Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
      Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
      All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
      Speaker: Dipali Sashital, PhD
      Presented at: CRISPR Virtual Event Series 2018
      3
      SEP 13, 2018 | 12:00 PM
      Outer Membrane Remodeling of Attenuated Salmonella to Create a Mucosal Vaccine Against Acinetobacter Baumannii
      Outer Membrane Remodeling of Attenuated Salmonella to Create a...
      While rapid identification of pathogens, novel therapeutic interventions, and passive immunization have critical roles in disease control, none can substitute for pre-existing protective immu...
      Speaker: James Galen, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      SEP 13, 2018 | 10:30 AM
      Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic Resistance
      Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic...
      Horizontal gene transfer (HGT) – or the non-genealogical transmission of DNA between organisms – is the dominant mode responsible for the spread of antibiotic resistance genes. Co...
      Speaker: Allison Lopatkin, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      3
      SEP 12, 2018 | 12:00 PM
      Tracking Antibiotic Resistance Across Space and Time
      Tracking Antibiotic Resistance Across Space and Time
      Horizontal gene transfer (HGT) has long been recognized as an important source of genetic diversity. In the clinical setting, HGT plays an important role in the spread of antibiotic resistanc...
      Speaker: Sean Conlan, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      MAY 10, 2018 | 6:00 AM
      C.E. CREDITS
      Multiplexed Precision Genome Editing with Trackable Genome-Integrated Barcodes in Yeast
      Multiplexed Precision Genome Editing with Trackable Genome-Integrated...
      Our understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess the phenotypic consequences of each perturbation. In this pr...
      Speaker: Kevin Roy, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      1
      APR 24, 2018 | 8:00 AM
      KASP genotyping - better by design
      KASP genotyping - better by design
      DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
      Speaker: Jonathan Curry, PhD
      Sponsored By: LGC Seracare
      13
      MAR 15, 2018 | 10:30 AM
      An Emerging Role of DNA Damage-Repair in Huntington's Disease
      An Emerging Role of DNA Damage-Repair in Huntington's Disease
      Machine learning approaches to pattern discovery in protein-protein interaction networks of Huntington’s disease brain and model organisms are revealing novel connections of the disease...
      Speaker: Animesh Ray, PhD
      Presented at: Neuroscience Virtual Event Series 2018
      2
      FEB 22, 2018 | 10:30 AM
      Illuminating the Druggable GPCR-ome
      Illuminating the Druggable GPCR-ome
      G-protein coupled receptors (GPCRs) represent the single largest class of druggable targets in the human genome. Of the 390 or so druggable and non-olfactory human GPCRs there exist many whic...
      Speaker: Bryan L. Roth, MD, PhD
      Presented at: Drug Discovery Virtual Event Series 2018
      1
      NOV 14, 2017 | 9:00 AM
      WEBINAR: CRISPRa tools for transcriptional activation studies
      WEBINAR: CRISPRa tools for transcriptional activation studies
      DATE: November 14, 2017TIME: 09:00am PST, 12:00pm ESTThe CRISPR-Cas9 system has been adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and av...
      Speaker: Žaklina Strezoska , Benedict Cross
      Sponsored By: Dharmacon, a Horizon Discovery Group Company
      11 4
      AUG 22, 2017 | 9:00 AM
      WEBINAR: A virtual workshop for precise HDR-mediated genome engineering with CRISPR-Cas9
      WEBINAR: A virtual workshop for precise HDR-mediated genome engineering...
      DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
      Speaker: Maren Mayer Gross
      Sponsored By: Dharmacon
      12 5
      JUL 01, 2017 | 12:00 AM
      New Tools for improved CRISPR gene-editing in stem cells
      New Tools for improved CRISPR gene-editing in stem cells
      The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
      Speaker: James Kehler VMD, PhD
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      Highly efficient stem cell engineering via Cas9 protein transfection
      Highly efficient stem cell engineering via Cas9 protein transfection
      CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering.  The delivery of Cas9 plasmid DNA or mR...
      Speaker: Xiquan Liang
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      2
      JUN 28, 2017 | 7:00 AM
      Analytical methods to measure empty and full AAV particles
      Analytical methods to measure empty and full AAV particles
      DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
      Speaker: Christine LeBec, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences
      9 2
      MAY 10, 2017 | 4:00 PM
      Ensuring successful protein expression through proper plasmid purification
      Ensuring successful protein expression through proper plasmid...
      Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
      Speaker: Sasha Vlassov, PhD
      Presented at: 2017 Gibco ExpressionWorld
      3
      MAY 10, 2017 | 3:00 PM
      Large scale serum free suspension lentiviral production for cell and gene therapy application
      Large scale serum free suspension lentiviral production for cell...
      Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
      Speaker: Yongchang Ji, PhD.
      Presented at: 2017 Gibco ExpressionWorld
      4 1
      MAY 10, 2017 | 8:00 AM
      Optimisation of UCB's Transient Expression Platforms
      Optimisation of UCB's Transient Expression Platforms
      Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
      Speaker: Christina Gordon
      Presented at: 2017 Gibco ExpressionWorld
      4
      APR 05, 2017 | 7:15 AM
      Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
      Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
      CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
      Speaker: John Schiel, Ph.D.
      Presented at: Protein Research Live
      5
      APR 05, 2017 | 6:00 AM
      A workflow for CRISPR-Cas9 high throughput arrayed screening with synthetic crRNA
      A workflow for CRISPR-Cas9 high throughput arrayed screening with...
      There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
      Speaker: Louise Baskin
      Presented at: Protein Research Live
      20
    NOV 19, 2019 | 8:00 AM
    Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows
    Overcoming the Hurdles of Density Gradient Ultracentrifugation...
    DATE: November 19, 2019TIME: 8:00am PTCentrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of discipline...
    Speaker: Ross VerHeul
    Sponsored By: Beckman Coulter Life Sciences
    7 1
    NOV 05, 2019 | 9:00 AM
    Minicircle DNA: a new material for CAR-T and AAV vector particles - its development and analysis
    Minicircle DNA: a new material for CAR-T and AAV vector particles...
    DATE: November 5, 2019TIME: 9:00am PT, 12:00pm ET New results have highlighted the advantages of minicircles over plasmids as they avoid unnecessary bacterial backbone sequences...
    Speaker: Martin Schleef, PhD
    Sponsored By: SCIEX
    4
    OCT 10, 2019 | 6:00 AM
    Highly-efficient and -specific genome editing with Cas9 nickase ribonucleoproteins in Primary T-cells
    Highly-efficient and -specific genome editing with Cas9 nickase...
    In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases wh...
    Speaker: Jacob Lamberth
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2019
    Sponsored By: MilliporeSigma
    1
    SEP 12, 2019 | 10:30 AM
    Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
    Analysis of human gut microbiomes and antibiotic resistomes of...
    The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
    Speaker: Jonathan Jacobs, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2019
    Sponsored By: QIAGEN
    5
    SEP 12, 2019 | 10:00 AM
    High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS
    High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV)...
    DATE: September 12, 2019TIME: 10:00am PDT, 1:00pm EDT...
    Speaker: Tingting Li, MS
    Sponsored By: SCIEX
    2
     
     
  • JAN 31, 2019 | 7:30 AM
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
    Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
    Speaker: A.C. Matin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    1
    OCT 03, 2018 | 12:00 PM
    Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
    Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
    All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
    Speaker: Dipali Sashital, PhD
    Presented at: CRISPR Virtual Event Series 2018
    3
    SEP 13, 2018 | 12:00 PM
    Outer Membrane Remodeling of Attenuated Salmonella to Create a Mucosal Vaccine Against Acinetobacter Baumannii
    Outer Membrane Remodeling of Attenuated Salmonella to Create a...
    While rapid identification of pathogens, novel therapeutic interventions, and passive immunization have critical roles in disease control, none can substitute for pre-existing protective immu...
    Speaker: James Galen, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
    SEP 13, 2018 | 10:30 AM
    Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic Resistance
    Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic...
    Horizontal gene transfer (HGT) – or the non-genealogical transmission of DNA between organisms – is the dominant mode responsible for the spread of antibiotic resistance genes. Co...
    Speaker: Allison Lopatkin, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    3
    SEP 12, 2018 | 12:00 PM
    Tracking Antibiotic Resistance Across Space and Time
    Tracking Antibiotic Resistance Across Space and Time
    Horizontal gene transfer (HGT) has long been recognized as an important source of genetic diversity. In the clinical setting, HGT plays an important role in the spread of antibiotic resistanc...
    Speaker: Sean Conlan, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
     
     
  • MAY 10, 2018 | 6:00 AM
    C.E. CREDITS
    Multiplexed Precision Genome Editing with Trackable Genome-Integrated Barcodes in Yeast
    Multiplexed Precision Genome Editing with Trackable Genome-Integrated...
    Our understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess the phenotypic consequences of each perturbation. In this pr...
    Speaker: Kevin Roy, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    1
    APR 24, 2018 | 8:00 AM
    KASP genotyping - better by design
    KASP genotyping - better by design
    DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
    Speaker: Jonathan Curry, PhD
    Sponsored By: LGC Seracare
    13
    MAR 15, 2018 | 10:30 AM
    An Emerging Role of DNA Damage-Repair in Huntington's Disease
    An Emerging Role of DNA Damage-Repair in Huntington's Disease
    Machine learning approaches to pattern discovery in protein-protein interaction networks of Huntington’s disease brain and model organisms are revealing novel connections of the disease...
    Speaker: Animesh Ray, PhD
    Presented at: Neuroscience Virtual Event Series 2018
    2
    FEB 22, 2018 | 10:30 AM
    Illuminating the Druggable GPCR-ome
    Illuminating the Druggable GPCR-ome
    G-protein coupled receptors (GPCRs) represent the single largest class of druggable targets in the human genome. Of the 390 or so druggable and non-olfactory human GPCRs there exist many whic...
    Speaker: Bryan L. Roth, MD, PhD
    Presented at: Drug Discovery Virtual Event Series 2018
    1
    NOV 14, 2017 | 9:00 AM
    WEBINAR: CRISPRa tools for transcriptional activation studies
    WEBINAR: CRISPRa tools for transcriptional activation studies
    DATE: November 14, 2017TIME: 09:00am PST, 12:00pm ESTThe CRISPR-Cas9 system has been adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and av...
    Speaker: Žaklina Strezoska , Benedict Cross
    Sponsored By: Dharmacon, a Horizon Discovery Group Company
    11 4
     
     
  • AUG 22, 2017 | 9:00 AM
    WEBINAR: A virtual workshop for precise HDR-mediated genome engineering with CRISPR-Cas9
    WEBINAR: A virtual workshop for precise HDR-mediated genome engineering...
    DATE: August 22, 2017TIME: 9:00am PT, 12:00pm ETThe use of CRISPR-Cas9 to create targeted double-strand breaks in genomic DNA has greatly simplified strategies for precise gen...
    Speaker: Maren Mayer Gross
    Sponsored By: Dharmacon
    12 5
    JUL 01, 2017 | 12:00 AM
    New Tools for improved CRISPR gene-editing in stem cells
    New Tools for improved CRISPR gene-editing in stem cells
    The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
    Speaker: James Kehler VMD, PhD
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    2
    JUL 01, 2017 | 12:00 AM
    Highly efficient stem cell engineering via Cas9 protein transfection
    Highly efficient stem cell engineering via Cas9 protein transfection
    CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering.  The delivery of Cas9 plasmid DNA or mR...
    Speaker: Xiquan Liang
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    2
    JUN 28, 2017 | 7:00 AM
    Analytical methods to measure empty and full AAV particles
    Analytical methods to measure empty and full AAV particles
    DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
    Speaker: Christine LeBec, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences
    9 2
    MAY 10, 2017 | 4:00 PM
    Ensuring successful protein expression through proper plasmid purification
    Ensuring successful protein expression through proper plasmid...
    Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
    Speaker: Sasha Vlassov, PhD
    Presented at: 2017 Gibco ExpressionWorld
    3
     
     
  • MAY 10, 2017 | 3:00 PM
    Large scale serum free suspension lentiviral production for cell and gene therapy application
    Large scale serum free suspension lentiviral production for cell...
    Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
    Speaker: Yongchang Ji, PhD.
    Presented at: 2017 Gibco ExpressionWorld
    4 1
    MAY 10, 2017 | 8:00 AM
    Optimisation of UCB's Transient Expression Platforms
    Optimisation of UCB's Transient Expression Platforms
    Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
    Speaker: Christina Gordon
    Presented at: 2017 Gibco ExpressionWorld
    4
    APR 05, 2017 | 7:15 AM
    Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
    Optimizing homology-directed repair (HDR) results with CRISPR-Cas9
    CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
    Speaker: John Schiel, Ph.D.
    Presented at: Protein Research Live
    5
    APR 05, 2017 | 6:00 AM
    A workflow for CRISPR-Cas9 high throughput arrayed screening with synthetic crRNA
    A workflow for CRISPR-Cas9 high throughput arrayed screening with...
    There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
    Speaker: Louise Baskin
    Presented at: Protein Research Live
    20
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