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    Plasmid

    A plasmid is a small circular piece of DNA, wholly separate from the genomic DNA, most commonly found in Bacteria and Archaea. Often encoding event-restricted genes that aid in organism survival, they can be divested if need be. Plasmids are often used in the biotech industry in order to clone recombinant proteins for expression by E. coli.

    Webinars

    • JUN 08, 2021 | 9:00 AM
      Innovations in Vaccine Research and Development
      Innovations in Vaccine Research and Development
      Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
      Speaker: Laura Chapman , Ketil Pedersen, Ph.D , Alexander "Sasha" Vlassov , Matt Mckenna
      Sponsored By: Thermo Fisher Scientific
      28 4
      APR 20, 2021 | 4:30 PM
      Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene Therapy Research
      Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene...
      Interest in cell and gene therapy-based disease prevention and treatment has increased rapidly over the last few decades, however there are still many hurdles to overcome and further progres...
      Speaker: Andrea O'Hara, PhD
      Presented at: Genetics Week Virtual Event Series 2021
      Sponsored By: GENEWIZ
      3
      FEB 24, 2021 | 12:00 AM
      The development and application of TRUPATH biosensors to the study of GPCR pharmacology and functional selectivity
      The development and application of TRUPATH biosensors to the study...
      G-protein-coupled receptors (GPCRs) remain major drug targets, despite an incomplete understanding of how they signal through 16 non-visual G-protein signal transducers (collectively named t...
      Speaker: Reid Olsen, PhD, Pharmacology, Head of GPCR Pharmacology
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      4
      DEC 15, 2020 | 10:00 AM
      Prep for Success: From human microbiome exploration to advanced SARS-CoV-2 testing in wastewater
      Prep for Success: From human microbiome exploration to advanced...
      DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
      Speaker: Sasha Vlassov, PhD , Jasti Madhu , Emily Zeringer
      Sponsored By: Thermo Fisher Scientific
      13
      NOV 19, 2020 | 8:00 AM
      From discovery to functional assays: therapeutic antibody research by Miltenyi Biotec
      From discovery to functional assays: therapeutic antibody research...
      Date: November 19, 2020 Time: 8:00am PST, 11:00am EST Monoclonal antibody-based drugs have shown great potential as a treatment for several cancers. At Miltenyi Biotec, we offer a complete wo...
      Speaker: Dr. Susanne Bethke
      Sponsored By: Miltenyi Biotec
      9
      SEP 30, 2020 | 12:00 PM
      Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
      Efficient Generation of Gene-edited Mouse Models and Cell Lines...
      The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
      Speaker: Gurpreet Balrey, PhD , Peter Romanienko, PhD
      Presented at: CRISPR Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      4
      SEP 10, 2020 | 12:00 AM
      Challenges in tracking horizontal gene transfer of antimicrobial resistance
      Challenges in tracking horizontal gene transfer of antimicrobial...
      Focusing on the urgent clinical problem of increasing carbapenem resistance in Enterobacteriaceae we have been evaluating detection methods in clinical microbiology and molecular transmissio...
      Speaker: Amy Mathers, MD, D(ABMM)
      Presented at: Microbiology Week Virtual Event Series 2020
      4
      SEP 10, 2020 | 12:00 AM
      Identifying and linking antimicrobial resistance genes in metagenomes with new DNA sequencing technologies
      Identifying and linking antimicrobial resistance genes in metagenomes...
      The transfer of antimicrobial resistance genes (ARG) to pathogenic microbes is a major concern in modern medicine. Antibiotic therapies are often rendered ineffective by horizontal acquisiti...
      Speaker: Derek Bickhart, PhD
      Presented at: Microbiology Week Virtual Event Series 2020
      4
      JUN 24, 2020 | 8:00 AM
      CRISPR-Cas assisted genetics in intestinal organoids
      CRISPR-Cas assisted genetics in intestinal organoids
      DATE: June 24, 2020 TIME: 8:00am PT The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D...
      Speaker: Bon-Kyoung Koo, PhD
      Sponsored By: GenScript
      33 5
      JUN 02, 2020 | 9:00 AM
      Osmolality Testing: Providing Unique Process Control for Downstream Operations
      Osmolality Testing: Providing Unique Process Control for Downstream...
      DATE: June 2, 2020 TIME: 9:00am PT, 12:00pm ET Osmolality is a well-established parameter for upstream bioprocessing, formulation, analytical development and QC applications throughout the m...
      Speaker: Alois Jungbauer, PhD , Kristeena Wright, PhD
      Sponsored By: Advanced Instruments
      11 1
      DEC 11, 2019 | 7:00 AM
      A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
      A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis...
      DATE: December 11, 2019TIME: 7:00am PST, 10:00am EST An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key...
      Speaker: Colleen Reed
      Sponsored By: SCIEX
      7
      DEC 10, 2019 | 7:00 AM
      Proven Answers for Protein, Vaccine and Gene Therapy Analysis
      Proven Answers for Protein, Vaccine and Gene Therapy Analysis
      DATE: December 11, 2019 TIME: 7:00am PST, 10:00am EST Rapid growth in the cell and gene therapy industry has generated an urgent need for robust analytics around characterization of both vec...
      Speaker: Mukesh Malik, PhD
      Sponsored By: SCIEX
      8 1
      NOV 19, 2019 | 8:00 AM
      Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows
      Overcoming the Hurdles of Density Gradient Ultracentrifugation...
      DATE: November 19, 2019TIME: 8:00am PTCentrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of discipline...
      Speaker: Ross VerHeul
      Sponsored By: Beckman Coulter Life Sciences
      7 1
      NOV 05, 2019 | 9:00 AM
      Minicircle DNA: a new material for CAR-T and AAV vector particles - its development and analysis
      Minicircle DNA: a new material for CAR-T and AAV vector particles...
      DATE: November 5, 2019TIME: 9:00am PT, 12:00pm ET New results have highlighted the advantages of minicircles over plasmids as they avoid unnecessary bacterial backbone sequences...
      Speaker: Martin Schleef, PhD
      Sponsored By: SCIEX
      4
      OCT 10, 2019 | 6:00 AM
      Highly-efficient and -specific genome editing with Cas9 nickase ribonucleoproteins in Primary T-cells
      Highly-efficient and -specific genome editing with Cas9 nickase...
      In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases wh...
      Speaker: Jacob Lamberth
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2019
      Sponsored By: MilliporeSigma
      1
      SEP 12, 2019 | 10:30 AM
      Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
      Analysis of human gut microbiomes and antibiotic resistomes of...
      The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
      Speaker: Jonathan Jacobs, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2019
      Sponsored By: QIAGEN
      5
      SEP 12, 2019 | 10:00 AM
      High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS
      High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV)...
      DATE: September 12, 2019TIME: 10:00am PDT, 1:00pm EDT...
      Speaker: Tingting Li, MS
      Sponsored By: SCIEX
      2
      JAN 31, 2019 | 7:30 AM
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
      Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
      Speaker: A.C. Matin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      1
      OCT 03, 2018 | 12:00 PM
      Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
      Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
      All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
      Speaker: Dipali Sashital, PhD
      Presented at: CRISPR Virtual Event Series 2018
      3
      SEP 13, 2018 | 12:00 PM
      Outer Membrane Remodeling of Attenuated Salmonella to Create a Mucosal Vaccine Against Acinetobacter Baumannii
      Outer Membrane Remodeling of Attenuated Salmonella to Create a...
      While rapid identification of pathogens, novel therapeutic interventions, and passive immunization have critical roles in disease control, none can substitute for pre-existing protective immu...
      Speaker: James Galen, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      SEP 13, 2018 | 10:30 AM
      Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic Resistance
      Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic...
      Horizontal gene transfer (HGT) – or the non-genealogical transmission of DNA between organisms – is the dominant mode responsible for the spread of antibiotic resistance genes. Co...
      Speaker: Allison Lopatkin, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      3
      SEP 12, 2018 | 12:00 PM
      Tracking Antibiotic Resistance Across Space and Time
      Tracking Antibiotic Resistance Across Space and Time
      Horizontal gene transfer (HGT) has long been recognized as an important source of genetic diversity. In the clinical setting, HGT plays an important role in the spread of antibiotic resistanc...
      Speaker: Sean Conlan, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2018
      1
      MAY 10, 2018 | 6:00 AM
      C.E. CREDITS
      Multiplexed Precision Genome Editing with Trackable Genome-Integrated Barcodes in Yeast
      Multiplexed Precision Genome Editing with Trackable Genome-Integrated...
      Our understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess the phenotypic consequences of each perturbation. In this pr...
      Speaker: Kevin Roy, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      1
      APR 24, 2018 | 8:00 AM
      KASP genotyping - better by design
      KASP genotyping - better by design
      DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
      Speaker: Jonathan Curry, PhD
      Sponsored By: LGC Seracare
      13
    JUN 08, 2021 | 9:00 AM
    Innovations in Vaccine Research and Development
    Innovations in Vaccine Research and Development
    Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
    Speaker: Laura Chapman , Ketil Pedersen, Ph.D , Alexander "Sasha" Vlassov , Matt Mckenna
    Sponsored By: Thermo Fisher Scientific
    28 4
    APR 20, 2021 | 4:30 PM
    Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene Therapy Research
    Advancing AAV: Novel Synthesis and Sequencing Solutions for Gene...
    Interest in cell and gene therapy-based disease prevention and treatment has increased rapidly over the last few decades, however there are still many hurdles to overcome and further progres...
    Speaker: Andrea O'Hara, PhD
    Presented at: Genetics Week Virtual Event Series 2021
    Sponsored By: GENEWIZ
    3
    FEB 24, 2021 | 12:00 AM
    The development and application of TRUPATH biosensors to the study of GPCR pharmacology and functional selectivity
    The development and application of TRUPATH biosensors to the study...
    G-protein-coupled receptors (GPCRs) remain major drug targets, despite an incomplete understanding of how they signal through 16 non-visual G-protein signal transducers (collectively named t...
    Speaker: Reid Olsen, PhD, Pharmacology, Head of GPCR Pharmacology
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    4
    DEC 15, 2020 | 10:00 AM
    Prep for Success: From human microbiome exploration to advanced SARS-CoV-2 testing in wastewater
    Prep for Success: From human microbiome exploration to advanced...
    DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
    Speaker: Sasha Vlassov, PhD , Jasti Madhu , Emily Zeringer
    Sponsored By: Thermo Fisher Scientific
    13
    NOV 19, 2020 | 8:00 AM
    From discovery to functional assays: therapeutic antibody research by Miltenyi Biotec
    From discovery to functional assays: therapeutic antibody research...
    Date: November 19, 2020 Time: 8:00am PST, 11:00am EST Monoclonal antibody-based drugs have shown great potential as a treatment for several cancers. At Miltenyi Biotec, we offer a complete wo...
    Speaker: Dr. Susanne Bethke
    Sponsored By: Miltenyi Biotec
    9
     
     
  • SEP 30, 2020 | 12:00 PM
    Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
    Efficient Generation of Gene-edited Mouse Models and Cell Lines...
    The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
    Speaker: Gurpreet Balrey, PhD , Peter Romanienko, PhD
    Presented at: CRISPR Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    4
    SEP 10, 2020 | 12:00 AM
    Challenges in tracking horizontal gene transfer of antimicrobial resistance
    Challenges in tracking horizontal gene transfer of antimicrobial...
    Focusing on the urgent clinical problem of increasing carbapenem resistance in Enterobacteriaceae we have been evaluating detection methods in clinical microbiology and molecular transmissio...
    Speaker: Amy Mathers, MD, D(ABMM)
    Presented at: Microbiology Week Virtual Event Series 2020
    4
    SEP 10, 2020 | 12:00 AM
    Identifying and linking antimicrobial resistance genes in metagenomes with new DNA sequencing technologies
    Identifying and linking antimicrobial resistance genes in metagenomes...
    The transfer of antimicrobial resistance genes (ARG) to pathogenic microbes is a major concern in modern medicine. Antibiotic therapies are often rendered ineffective by horizontal acquisiti...
    Speaker: Derek Bickhart, PhD
    Presented at: Microbiology Week Virtual Event Series 2020
    4
    JUN 24, 2020 | 8:00 AM
    CRISPR-Cas assisted genetics in intestinal organoids
    CRISPR-Cas assisted genetics in intestinal organoids
    DATE: June 24, 2020 TIME: 8:00am PT The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D...
    Speaker: Bon-Kyoung Koo, PhD
    Sponsored By: GenScript
    33 5
    JUN 02, 2020 | 9:00 AM
    Osmolality Testing: Providing Unique Process Control for Downstream Operations
    Osmolality Testing: Providing Unique Process Control for Downstream...
    DATE: June 2, 2020 TIME: 9:00am PT, 12:00pm ET Osmolality is a well-established parameter for upstream bioprocessing, formulation, analytical development and QC applications throughout the m...
    Speaker: Alois Jungbauer, PhD , Kristeena Wright, PhD
    Sponsored By: Advanced Instruments
    11 1
     
     
  • DEC 11, 2019 | 7:00 AM
    A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
    A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis...
    DATE: December 11, 2019TIME: 7:00am PST, 10:00am EST An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key...
    Speaker: Colleen Reed
    Sponsored By: SCIEX
    7
    DEC 10, 2019 | 7:00 AM
    Proven Answers for Protein, Vaccine and Gene Therapy Analysis
    Proven Answers for Protein, Vaccine and Gene Therapy Analysis
    DATE: December 11, 2019 TIME: 7:00am PST, 10:00am EST Rapid growth in the cell and gene therapy industry has generated an urgent need for robust analytics around characterization of both vec...
    Speaker: Mukesh Malik, PhD
    Sponsored By: SCIEX
    8 1
    NOV 19, 2019 | 8:00 AM
    Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows
    Overcoming the Hurdles of Density Gradient Ultracentrifugation...
    DATE: November 19, 2019TIME: 8:00am PTCentrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of discipline...
    Speaker: Ross VerHeul
    Sponsored By: Beckman Coulter Life Sciences
    7 1
    NOV 05, 2019 | 9:00 AM
    Minicircle DNA: a new material for CAR-T and AAV vector particles - its development and analysis
    Minicircle DNA: a new material for CAR-T and AAV vector particles...
    DATE: November 5, 2019TIME: 9:00am PT, 12:00pm ET New results have highlighted the advantages of minicircles over plasmids as they avoid unnecessary bacterial backbone sequences...
    Speaker: Martin Schleef, PhD
    Sponsored By: SCIEX
    4
    OCT 10, 2019 | 6:00 AM
    Highly-efficient and -specific genome editing with Cas9 nickase ribonucleoproteins in Primary T-cells
    Highly-efficient and -specific genome editing with Cas9 nickase...
    In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases wh...
    Speaker: Jacob Lamberth
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2019
    Sponsored By: MilliporeSigma
    1
     
     
  • SEP 12, 2019 | 10:30 AM
    Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
    Analysis of human gut microbiomes and antibiotic resistomes of...
    The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
    Speaker: Jonathan Jacobs, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2019
    Sponsored By: QIAGEN
    5
    SEP 12, 2019 | 10:00 AM
    High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV) Capsid Proteins using CE-SDS
    High Sensitivity Purity Analysis of Adeno-Associated Virus (AAV)...
    DATE: September 12, 2019TIME: 10:00am PDT, 1:00pm EDT...
    Speaker: Tingting Li, MS
    Sponsored By: SCIEX
    2
    JAN 31, 2019 | 7:30 AM
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
    Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
    Speaker: A.C. Matin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    1
    OCT 03, 2018 | 12:00 PM
    Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
    Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
    All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
    Speaker: Dipali Sashital, PhD
    Presented at: CRISPR Virtual Event Series 2018
    3
    SEP 13, 2018 | 12:00 PM
    Outer Membrane Remodeling of Attenuated Salmonella to Create a Mucosal Vaccine Against Acinetobacter Baumannii
    Outer Membrane Remodeling of Attenuated Salmonella to Create a...
    While rapid identification of pathogens, novel therapeutic interventions, and passive immunization have critical roles in disease control, none can substitute for pre-existing protective immu...
    Speaker: James Galen, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
     
     
  • SEP 13, 2018 | 10:30 AM
    Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic Resistance
    Understanding and Inhibiting Horizontal Gene Transfer of Antibiotic...
    Horizontal gene transfer (HGT) – or the non-genealogical transmission of DNA between organisms – is the dominant mode responsible for the spread of antibiotic resistance genes. Co...
    Speaker: Allison Lopatkin, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    3
    SEP 12, 2018 | 12:00 PM
    Tracking Antibiotic Resistance Across Space and Time
    Tracking Antibiotic Resistance Across Space and Time
    Horizontal gene transfer (HGT) has long been recognized as an important source of genetic diversity. In the clinical setting, HGT plays an important role in the spread of antibiotic resistanc...
    Speaker: Sean Conlan, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2018
    1
    MAY 10, 2018 | 6:00 AM
    C.E. CREDITS
    Multiplexed Precision Genome Editing with Trackable Genome-Integrated Barcodes in Yeast
    Multiplexed Precision Genome Editing with Trackable Genome-Integrated...
    Our understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess the phenotypic consequences of each perturbation. In this pr...
    Speaker: Kevin Roy, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    1
    APR 24, 2018 | 8:00 AM
    KASP genotyping - better by design
    KASP genotyping - better by design
    DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
    Speaker: Jonathan Curry, PhD
    Sponsored By: LGC Seracare
    13
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