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    Primary Culture

    Primary Culture: the stage of the culture after the cells are isolated from the tissue and proliferated under the appropriate conditions until they occupy all of the available substrate.

    Webinars

    • MAY 10, 2017 | 7:00 AM
      Basic Concepts in Transient Expression using 293 and CHO cells
      Basic Concepts in Transient Expression using 293 and CHO cells
      HEK293 and CHO cells are the primary cell lines used in rapid, scalable suspension culture systems for transient protein expression. This presentation provides a primer on fundamental concept...
      Speaker: Henry Chiou, PhD
      Presented at: 2017 Gibco ExpressionWorld
      5
      MAY 01, 2017 | 8:00 AM
      Improved differentiation of stem cell-derived neurons and downstream applications through the reduction of progenitor proliferation with CultureOneâ„¢ Supplement
      Improved differentiation of stem cell-derived neurons and downstream...
      Neurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and drug screening.  Human PSCs derived neural stem...
      Speaker: David Kuninger, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      1
      APR 19, 2017 | 4:00 AM
      WEBINAR: Patient Derived Micro Tumor systems: Future Trends towards improved testing systems
      WEBINAR: Patient Derived Micro Tumor systems: Future Trends towards...
      DATE: April 20th, 2017TIME: 10:00AM SGT, 11:00AM KST, 12:00PM AEST, 2:00PM NZSTWith increasing breadth and depth of genomics studies across a range of cancers, it is now apparent that t...
      Speaker: Giridharan Periyasamy, PhD
      Sponsored By: Revvity
      8
      APR 13, 2017 | 7:30 AM
      The Essential Role of the Molecular Identification of Microorganisms in the Management of Chronic Infections
      The Essential Role of the Molecular Identification of Microorganisms...
      Microorganisms growing in biofilm phenotype are the cause of chronic infections.  Several universal properties of biofilm communities are they are overwhelmingly polymicrobial, difficult...
      Speaker: Randall Wolcott, MD
      Presented at: Molecular Diagnostics Virtual Event Series 2017
      3
      FEB 22, 2017 | 6:00 AM
      GenomeFIRST â„¢ A new paradigm for the return of genomic results
      GenomeFIRST â„¢ A new paradigm for the return of genomic results
      The starting point for an interaction between a patient and a clinician is almost always a set of clinical facts (aka a phenotype). Phenotype-first medicine is the standard way in which the p...
      Speaker: Marc Williams, MD
      Presented at: Precision Medicine Virtual Event Series 2017
      4
      DEC 14, 2016 | 7:30 AM
      Advances in modeling human diseases using induced pluripotent stem cell-derived cellular models
      Advances in modeling human diseases using induced pluripotent...
      DATE: December 14, 2016 TIME: 7:30am PT, 10:30am ET A major hurdle in developing relevant disease models for drug discovery is access to the healthy and diseased tissue of interest; induc...
      Speaker: T.K. Feaster, PhD , Kile Mangan, PhD
      Sponsored By: Cellular Dynamics, Cellular Dynamics
      7 1
      NOV 17, 2016 | 8:30 AM
      Simultaneous, single-cell visualization of RNA and Protein
      Simultaneous, single-cell visualization of RNA and Protein
      DATE: November 17, 2016 TIME: 8:30am PT, 11:30am ET The complex interactions between transcription, translation, and post-translational modifications are hidden from view in typ...
      Speaker: Sara Becker-Catania, PhD
      Sponsored By: eBioscience Business Unit, Thermo Fisher Scientific, eBioscience Business Unit, Thermo Fisher Scientific
      4
      NOV 16, 2016 | 4:00 PM
      Evolving your media system for modern stem cell applications
      Evolving your media system for modern stem cell applications
      Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeut...
      Speaker: Rhonda Newman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      4
      OCT 18, 2016 | 1:00 PM
      The Role of 3D Culture Methods in Retaining Native Biological Properties of Translational In Vitro Models
      The Role of 3D Culture Methods in Retaining Native Biological...
      Cell Sciences Innovation, Merck Research Laboratories, 2015 Galloping Hill Road, Kenilworth, NJ USA In vitro translational models play a critical role in the drug discovery process because t...
      Speaker: Richard Visconti, PhD
      Presented at: SyncD³ - Where drug discovery & development meet
      0
      OCT 18, 2016 | 11:45 AM
      Cell Models for Therapeutic Discovery - Leveraging Engineered, Primary and Stem Cells
      Cell Models for Therapeutic Discovery - Leveraging Engineered,...
      ...
      Speaker: David Piper, PhD
      Presented at: SyncD³ - Where drug discovery & development meet
      0
      SEP 20, 2016 | 7:00 AM
      Advanced Analysis of Complex Cell Models with the New Operetta CLS HCA System
      Advanced Analysis of Complex Cell Models with the New Operetta...
      DATE: September 20th, 2016TIME:  7:00AM PST, 10:00AM ETThere is a growing trend towards developing in vitro cell models that recapitulate the in vivo environment in basic research,...
      Speaker: Jacob Tesdorpf, PhD , Joe Trask
      Sponsored By: Revvity
      25
      AUG 30, 2016 | 8:00 AM
      Translating Pluripotent Stem Cell Therapies For Focal Brain Disorders
      Translating Pluripotent Stem Cell Therapies For Focal Brain Disorders
      A major challenge for the clinical application of pluripotent stem cell therapy for neurodegenerative diseases is large-scale manufacturing and cryopreservation of neurons and glia that can b...
      Speaker: Dustin Wakeman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      1
      AUG 30, 2016 | 8:00 AM
      Improved differentiation of human pluripotent stem cell-derived neurons through reduction of progenitor proliferation: impact on downstream applications
      Improved differentiation of human pluripotent stem cell-derived...
      Neurons derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are excellent resources for disease modeling and d...
      Speaker: David Kuninger, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      1
      AUG 30, 2016 | 8:00 AM
      The matrix equation -  solving for the best pluripotent stem cell culture goals
      The matrix equation - solving for the best pluripotent stem cell...
      Access this training to learn more about the evolution of pluripotent stem cell culture systems and which solution is best for your PSC culture goals....
      Speaker: Rhonda Newman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      0
      MAY 31, 2016 | 7:00 AM
      Protect your Cells with Proper Pipetting - How liquid handling influences your cell culture work
      Protect your Cells with Proper Pipetting - How liquid handling...
      DATE: May 31, 2016 TIME:  7am Pacific time, 10am Easter time, 4pm Central European Summer time  Pipetting is an every-day task in a cell culture lab. We pipette so often that we...
      Speaker: Jessica Wagener, PhD , Hanae Henke, PhD
      Sponsored By: Eppendorf, Eppendorf
      3 7
      FEB 24, 2016 | 1:00 PM
      From genomics to precision medicine: Uncovering and manipulating the genetic circuits underlying common disease
      From genomics to precision medicine: Uncovering and manipulating...
      Precision medicine requires understanding the mechanistic basis of complex disorders, and to precisely manipulate these mechanisms to better human health. This is partly enabled by the recent...
      Speaker: Manolis Kellis, PhD
      Presented at: Precision Medicine Virtual Event Series 2016
      2
      FEB 24, 2016 | 8:00 AM
      Keynote Presentation - Integrating the digital universe of data with consumer-driven mobile apps and large-scale panomics data to better understand, treat, and prevent disease
      Keynote Presentation - Integrating the digital universe of data...
      One of the primary goals of precision medicine is the aggregation and interpretation of deep, longitudinal patient-specific data in the context of the digital universe of information, using a...
      Speaker: Eric Schadt, PhD
      Presented at: Precision Medicine Virtual Event Series 2016
      2
      FEB 04, 2016 | 1:30 PM
      Small Refinements = Large Enhancements to Science and Animal Welfare
      Small Refinements = Large Enhancements to Science and Animal Welfare
      Science continues to evolve at a rapid pace. Our understanding of laboratory animal behavior and welfare is also growing. The biomedical research community continues to receive criticisms fro...
      Speaker: Letty Medina, DVM, DACLAM
      Presented at: Laboratory Animal Sciences Virtual Event Series 2016
      4
      FEB 05, 2015 | 10:30 AM
      C.E. CREDITS
      Effective Workplace Training for English Language Learners
      Effective Workplace Training for English Language Learners
      Effective workplace training in a research facility is an important key in ensuring smooth operations and high standards of animal care. Today's diverse workforce offers many benefits such a...
      Speaker: Paula Clifford, MLA, RLATG, CVT
      Presented at: Laboratory Animal Sciences Virtual Event Series 2015
      10
      OCT 29, 2014 | 12:00 PM
      Obesity, weight loss, and the microenvironment in basal-like breast cancer
      Obesity, weight loss, and the microenvironment in basal-like breast...
      Obesity is associated with an aggressive subtype of breast cancer called basal-like breast cancer (BBC). Using C3(1)-TAg mice, a genetically engineered mouse model that resembles human BBC, w...
      Speaker: Liza Makowski, PhD
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
      12
      OCT 09, 2014 | 7:00 AM
       Preventing Contamination in Cell Culture
      Preventing Contamination in Cell Culture
      Contamination is an everyday challenge in cell culture labs. Undetected or ignored contamination and misidentification of cell lines endanger the reliability and reproducibility of experiment...
      Speaker: Jessica Wagener, PhD
      Sponsored By: Eppendorf, Eppendorf
      62
    MAY 10, 2017 | 7:00 AM
    Basic Concepts in Transient Expression using 293 and CHO cells
    Basic Concepts in Transient Expression using 293 and CHO cells
    HEK293 and CHO cells are the primary cell lines used in rapid, scalable suspension culture systems for transient protein expression. This presentation provides a primer on fundamental concept...
    Speaker: Henry Chiou, PhD
    Presented at: 2017 Gibco ExpressionWorld
    5
    MAY 01, 2017 | 8:00 AM
    Improved differentiation of stem cell-derived neurons and downstream applications through the reduction of progenitor proliferation with CultureOneâ„¢ Supplement
    Improved differentiation of stem cell-derived neurons and downstream...
    Neurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and drug screening.  Human PSCs derived neural stem...
    Speaker: David Kuninger, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    1
    APR 19, 2017 | 4:00 AM
    WEBINAR: Patient Derived Micro Tumor systems: Future Trends towards improved testing systems
    WEBINAR: Patient Derived Micro Tumor systems: Future Trends towards...
    DATE: April 20th, 2017TIME: 10:00AM SGT, 11:00AM KST, 12:00PM AEST, 2:00PM NZSTWith increasing breadth and depth of genomics studies across a range of cancers, it is now apparent that t...
    Speaker: Giridharan Periyasamy, PhD
    Sponsored By: Revvity
    8
    APR 13, 2017 | 7:30 AM
    The Essential Role of the Molecular Identification of Microorganisms in the Management of Chronic Infections
    The Essential Role of the Molecular Identification of Microorganisms...
    Microorganisms growing in biofilm phenotype are the cause of chronic infections.  Several universal properties of biofilm communities are they are overwhelmingly polymicrobial, difficult...
    Speaker: Randall Wolcott, MD
    Presented at: Molecular Diagnostics Virtual Event Series 2017
    3
    FEB 22, 2017 | 6:00 AM
    GenomeFIRST â„¢ A new paradigm for the return of genomic results
    GenomeFIRST â„¢ A new paradigm for the return of genomic results
    The starting point for an interaction between a patient and a clinician is almost always a set of clinical facts (aka a phenotype). Phenotype-first medicine is the standard way in which the p...
    Speaker: Marc Williams, MD
    Presented at: Precision Medicine Virtual Event Series 2017
    4
     
     
  • DEC 14, 2016 | 7:30 AM
    Advances in modeling human diseases using induced pluripotent stem cell-derived cellular models
    Advances in modeling human diseases using induced pluripotent...
    DATE: December 14, 2016 TIME: 7:30am PT, 10:30am ET A major hurdle in developing relevant disease models for drug discovery is access to the healthy and diseased tissue of interest; induc...
    Speaker: T.K. Feaster, PhD , Kile Mangan, PhD
    Sponsored By: Cellular Dynamics, Cellular Dynamics
    7 1
    NOV 17, 2016 | 8:30 AM
    Simultaneous, single-cell visualization of RNA and Protein
    Simultaneous, single-cell visualization of RNA and Protein
    DATE: November 17, 2016 TIME: 8:30am PT, 11:30am ET The complex interactions between transcription, translation, and post-translational modifications are hidden from view in typ...
    Speaker: Sara Becker-Catania, PhD
    Sponsored By: eBioscience Business Unit, Thermo Fisher Scientific, eBioscience Business Unit, Thermo Fisher Scientific
    4
    NOV 16, 2016 | 4:00 PM
    Evolving your media system for modern stem cell applications
    Evolving your media system for modern stem cell applications
    Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeut...
    Speaker: Rhonda Newman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    4
    OCT 18, 2016 | 1:00 PM
    The Role of 3D Culture Methods in Retaining Native Biological Properties of Translational In Vitro Models
    The Role of 3D Culture Methods in Retaining Native Biological...
    Cell Sciences Innovation, Merck Research Laboratories, 2015 Galloping Hill Road, Kenilworth, NJ USA In vitro translational models play a critical role in the drug discovery process because t...
    Speaker: Richard Visconti, PhD
    Presented at: SyncD³ - Where drug discovery & development meet
    0
    OCT 18, 2016 | 11:45 AM
    Cell Models for Therapeutic Discovery - Leveraging Engineered, Primary and Stem Cells
    Cell Models for Therapeutic Discovery - Leveraging Engineered,...
    ...
    Speaker: David Piper, PhD
    Presented at: SyncD³ - Where drug discovery & development meet
    0
     
     
  • SEP 20, 2016 | 7:00 AM
    Advanced Analysis of Complex Cell Models with the New Operetta CLS HCA System
    Advanced Analysis of Complex Cell Models with the New Operetta...
    DATE: September 20th, 2016TIME:  7:00AM PST, 10:00AM ETThere is a growing trend towards developing in vitro cell models that recapitulate the in vivo environment in basic research,...
    Speaker: Jacob Tesdorpf, PhD , Joe Trask
    Sponsored By: Revvity
    25
    AUG 30, 2016 | 8:00 AM
    Translating Pluripotent Stem Cell Therapies For Focal Brain Disorders
    Translating Pluripotent Stem Cell Therapies For Focal Brain Disorders
    A major challenge for the clinical application of pluripotent stem cell therapy for neurodegenerative diseases is large-scale manufacturing and cryopreservation of neurons and glia that can b...
    Speaker: Dustin Wakeman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    1
    AUG 30, 2016 | 8:00 AM
    Improved differentiation of human pluripotent stem cell-derived neurons through reduction of progenitor proliferation: impact on downstream applications
    Improved differentiation of human pluripotent stem cell-derived...
    Neurons derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are excellent resources for disease modeling and d...
    Speaker: David Kuninger, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    1
    AUG 30, 2016 | 8:00 AM
    The matrix equation -  solving for the best pluripotent stem cell culture goals
    The matrix equation - solving for the best pluripotent stem cell...
    Access this training to learn more about the evolution of pluripotent stem cell culture systems and which solution is best for your PSC culture goals....
    Speaker: Rhonda Newman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    0
    MAY 31, 2016 | 7:00 AM
    Protect your Cells with Proper Pipetting - How liquid handling influences your cell culture work
    Protect your Cells with Proper Pipetting - How liquid handling...
    DATE: May 31, 2016 TIME:  7am Pacific time, 10am Easter time, 4pm Central European Summer time  Pipetting is an every-day task in a cell culture lab. We pipette so often that we...
    Speaker: Jessica Wagener, PhD , Hanae Henke, PhD
    Sponsored By: Eppendorf, Eppendorf
    3 7
     
     
  • FEB 24, 2016 | 1:00 PM
    From genomics to precision medicine: Uncovering and manipulating the genetic circuits underlying common disease
    From genomics to precision medicine: Uncovering and manipulating...
    Precision medicine requires understanding the mechanistic basis of complex disorders, and to precisely manipulate these mechanisms to better human health. This is partly enabled by the recent...
    Speaker: Manolis Kellis, PhD
    Presented at: Precision Medicine Virtual Event Series 2016
    2
    FEB 24, 2016 | 8:00 AM
    Keynote Presentation - Integrating the digital universe of data with consumer-driven mobile apps and large-scale panomics data to better understand, treat, and prevent disease
    Keynote Presentation - Integrating the digital universe of data...
    One of the primary goals of precision medicine is the aggregation and interpretation of deep, longitudinal patient-specific data in the context of the digital universe of information, using a...
    Speaker: Eric Schadt, PhD
    Presented at: Precision Medicine Virtual Event Series 2016
    2
    FEB 04, 2016 | 1:30 PM
    Small Refinements = Large Enhancements to Science and Animal Welfare
    Small Refinements = Large Enhancements to Science and Animal Welfare
    Science continues to evolve at a rapid pace. Our understanding of laboratory animal behavior and welfare is also growing. The biomedical research community continues to receive criticisms fro...
    Speaker: Letty Medina, DVM, DACLAM
    Presented at: Laboratory Animal Sciences Virtual Event Series 2016
    4
    FEB 05, 2015 | 10:30 AM
    C.E. CREDITS
    Effective Workplace Training for English Language Learners
    Effective Workplace Training for English Language Learners
    Effective workplace training in a research facility is an important key in ensuring smooth operations and high standards of animal care. Today's diverse workforce offers many benefits such a...
    Speaker: Paula Clifford, MLA, RLATG, CVT
    Presented at: Laboratory Animal Sciences Virtual Event Series 2015
    10
    OCT 29, 2014 | 12:00 PM
    Obesity, weight loss, and the microenvironment in basal-like breast cancer
    Obesity, weight loss, and the microenvironment in basal-like breast...
    Obesity is associated with an aggressive subtype of breast cancer called basal-like breast cancer (BBC). Using C3(1)-TAg mice, a genetically engineered mouse model that resembles human BBC, w...
    Speaker: Liza Makowski, PhD
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
    12
     
     
  • OCT 09, 2014 | 7:00 AM
     Preventing Contamination in Cell Culture
    Preventing Contamination in Cell Culture
    Contamination is an everyday challenge in cell culture labs. Undetected or ignored contamination and misidentification of cell lines endanger the reliability and reproducibility of experiment...
    Speaker: Jessica Wagener, PhD
    Sponsored By: Eppendorf, Eppendorf
    62
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