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    Direct PCR

    Direct PCR: Direct PCR allows for amplification directly from the tissue source or crude extracts, without the need for DNA purification. Omitting the multi-step process for preparing genomic DNA helps prevent sample loss, saves time, and reduces cost. Direct PCR vastly simplifies genotyping experiments, especially when the source material is limited.

    Webinars

    • SEP 26, 2018 | 12:00 PM
      3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
      3D Cell Cultures and Tissue Clearing: A High Content Confocal...
      With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
      Speaker: Michael Johnson, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      2
      MAY 03, 2018 | 11:00 AM
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
      DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
      Speaker: Sisi Li
      Sponsored By: Covaris
      5 2
      APR 24, 2018 | 8:00 AM
      KASP genotyping - better by design
      KASP genotyping - better by design
      DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
      Speaker: Jonathan Curry, PhD
      Sponsored By: LGC Seracare
      13
      SEP 28, 2017 | 1:30 PM
      The RRID Initiative, what is needed for scholarship in the 21st century
      The RRID Initiative, what is needed for scholarship in the 21st...
      The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
      Speaker: Anita Bandrowski, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 12:00 PM
      Back to basics: Fundamental concepts and special considerations in RNA isolation
      Back to basics: Fundamental concepts and special considerations...
      How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
      Speaker: Abhishek Sharma
      Presented at: Cell Biology Virtual Event Series 2017
      2
      SEP 28, 2017 | 10:30 AM
      Engineering stem cell biology for disease modelling and therapeutics
      Engineering stem cell biology for disease modelling and therapeutics
      Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
      Speaker: Amr Abdeen, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 9:00 AM
      Fine tuning of myosin activity shapes actomyosin network organization and tissue contractility of the C. elegans spermatheca
      Fine tuning of myosin activity shapes actomyosin network organization...
      Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
      Speaker: Alison Wirshing, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 7:30 AM
      Keynote Presentation: RNA-targeting CRISPR and application to diseases
      Keynote Presentation: RNA-targeting CRISPR and application to...
      I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
      Speaker: Gene Yeo, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 6:00 AM
      The axolotl salamander as a model for nerve-dependent regeneration
      The axolotl salamander as a model for nerve-dependent regeneration
      The Mexican axolotl (Ambystoma mexicanum) is an aquatic salamander that possesses some of the most astonishing regenerative abilities found in nature.  Able to fully regenerate amputated...
      Speaker: Johanna Farkas, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 1:30 PM
      Evolutionary Cell Biology is a powerful paradigm to study complex biological systems
      Evolutionary Cell Biology is a powerful paradigm to study complex...
      Although the cell is commonly referred to as “the most basic unit of life”, it is actually so complex that despite over 350 years of research we are still far from fully understan...
      Speaker: Idan Frumkin, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 12:00 PM
      CRISPR-Cas9 genome engineering: Experimental Design and Applications
      CRISPR-Cas9 genome engineering: Experimental Design and Applications
      The manipulation of the genome to study disease, evaluate drug targets and to stratify patient populations is now a widely accepted concept in the field. At Horizon Discovery, genome engineer...
      Speaker: Danielle Folkard
      Presented at: Cell Biology Virtual Event Series 2017
      Sponsored By: Horizon Discovery
      2
      SEP 27, 2017 | 9:00 AM
      Regulation of actin during cell migration by nucleotide coding sequence and arginylation
      Regulation of actin during cell migration by nucleotide coding...
      Actin cytoskeleton drives cell migration in muscle and non-muscle cells and plays a major role in such diverse processes as tissue morphogenesis, muscle contraction, and cancer metastases. In...
      Speaker: Anna Kashina, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 7:30 AM
      Keynote Presentation: What Microscopy Can Tell Us About the Life of an mRNA in Living Cells
      Keynote Presentation: What Microscopy Can Tell Us About the Life...
      Einstein researcher Robert Singer, Ph.D., discusses a breakthrough in microscopy that is allowing scientists to track messenger RNA in living cells in real time. The study, published in the S...
      Speaker: Robert Singer, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 6:00 AM
      Regulation of microtubule plus-end dynamics during axon guidance and cell migration
      Regulation of microtubule plus-end dynamics during axon guidance...
      A fundamental question in neuronal development is how growth cone cytoskeletal dynamics are coordinated to promote accurate axonal navigation. To address this question, we focus on microtubul...
      Speaker: Laura Lowery, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 13, 2017 | 1:30 PM
      Integration of the Simplexa C. difficile Direct assay in a two-step algorithm for the laboratory diagnosis of C. difficile
      Integration of the Simplexa C. difficile Direct assay in a two-step...
      Clostridium difficile is a major pathogen responsible for infectious diarrhea in healthcare settings. This presentation will review the data from a study comparing the new Simplexa® C. di...
      Speaker: Dr. James Snyder
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      Sponsored By: Diasorin
      2
      MAY 11, 2017 | 12:00 PM
      New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology
      New multiplexed nCounter® PlexSet™ Reagents- an alternative to...
      The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme...
      Speaker: Christian Lytle
      Presented at: Genetics and Genomics Virtual Event Series 2017
      Sponsored By: NanoString Technologies
      1
      NOV 08, 2016 | 10:00 AM
      The genetics of aquaculture: Genotyping with GBS and arrays for species identification and trait improvement
      The genetics of aquaculture: Genotyping with GBS and arrays for...
      DATE: November 8, 2016 TIME: 10:00am PT, 1:00pm ET Stock and individual identification of Coho and Chinook Salmon in British Columbia via amplicon sequencing We have assemble...
      Speaker: Terry Beacham, PhD , Yniv Palti, PhD
      Sponsored By: Affymetrix, part of Thermo Fisher Scientific, Affymetrix, part of Thermo Fisher Scientific
      5 1
      NOV 02, 2016 | 10:30 AM
      Tough Targets. Simple Genotyping. Using HRM as a direct approach in research applications.
      Tough Targets. Simple Genotyping. Using HRM as a direct approach...
      Are there DNA targets in your lab that are difficult to genotype using standard PCR or sequencing?Do you ever need to genotype a single sample, and you don’t want to wait to batch sam...
      Speaker: Brian McNally, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      2
      OCT 01, 2015 | 12:00 PM
      Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR
      Detection and sequencing of rare mutations using mutation enrichment...
      Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challengin...
      Speaker: Mike Makrigiorgos, PhD, DABMP
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      2
    SEP 26, 2018 | 12:00 PM
    3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
    3D Cell Cultures and Tissue Clearing: A High Content Confocal...
    With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
    Speaker: Michael Johnson, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    2
    MAY 03, 2018 | 11:00 AM
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    Speaker: Sisi Li
    Sponsored By: Covaris
    5 2
    APR 24, 2018 | 8:00 AM
    KASP genotyping - better by design
    KASP genotyping - better by design
    DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
    Speaker: Jonathan Curry, PhD
    Sponsored By: LGC Seracare
    13
    SEP 28, 2017 | 1:30 PM
    The RRID Initiative, what is needed for scholarship in the 21st century
    The RRID Initiative, what is needed for scholarship in the 21st...
    The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
    Speaker: Anita Bandrowski, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 12:00 PM
    Back to basics: Fundamental concepts and special considerations in RNA isolation
    Back to basics: Fundamental concepts and special considerations...
    How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
    Speaker: Abhishek Sharma
    Presented at: Cell Biology Virtual Event Series 2017
    2
     
     
  • SEP 28, 2017 | 10:30 AM
    Engineering stem cell biology for disease modelling and therapeutics
    Engineering stem cell biology for disease modelling and therapeutics
    Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
    Speaker: Amr Abdeen, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 9:00 AM
    Fine tuning of myosin activity shapes actomyosin network organization and tissue contractility of the C. elegans spermatheca
    Fine tuning of myosin activity shapes actomyosin network organization...
    Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
    Speaker: Alison Wirshing, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 7:30 AM
    Keynote Presentation: RNA-targeting CRISPR and application to diseases
    Keynote Presentation: RNA-targeting CRISPR and application to...
    I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
    Speaker: Gene Yeo, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 6:00 AM
    The axolotl salamander as a model for nerve-dependent regeneration
    The axolotl salamander as a model for nerve-dependent regeneration
    The Mexican axolotl (Ambystoma mexicanum) is an aquatic salamander that possesses some of the most astonishing regenerative abilities found in nature.  Able to fully regenerate amputated...
    Speaker: Johanna Farkas, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 1:30 PM
    Evolutionary Cell Biology is a powerful paradigm to study complex biological systems
    Evolutionary Cell Biology is a powerful paradigm to study complex...
    Although the cell is commonly referred to as “the most basic unit of life”, it is actually so complex that despite over 350 years of research we are still far from fully understan...
    Speaker: Idan Frumkin, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
     
     
  • SEP 27, 2017 | 12:00 PM
    CRISPR-Cas9 genome engineering: Experimental Design and Applications
    CRISPR-Cas9 genome engineering: Experimental Design and Applications
    The manipulation of the genome to study disease, evaluate drug targets and to stratify patient populations is now a widely accepted concept in the field. At Horizon Discovery, genome engineer...
    Speaker: Danielle Folkard
    Presented at: Cell Biology Virtual Event Series 2017
    Sponsored By: Horizon Discovery
    2
    SEP 27, 2017 | 9:00 AM
    Regulation of actin during cell migration by nucleotide coding sequence and arginylation
    Regulation of actin during cell migration by nucleotide coding...
    Actin cytoskeleton drives cell migration in muscle and non-muscle cells and plays a major role in such diverse processes as tissue morphogenesis, muscle contraction, and cancer metastases. In...
    Speaker: Anna Kashina, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 7:30 AM
    Keynote Presentation: What Microscopy Can Tell Us About the Life of an mRNA in Living Cells
    Keynote Presentation: What Microscopy Can Tell Us About the Life...
    Einstein researcher Robert Singer, Ph.D., discusses a breakthrough in microscopy that is allowing scientists to track messenger RNA in living cells in real time. The study, published in the S...
    Speaker: Robert Singer, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 6:00 AM
    Regulation of microtubule plus-end dynamics during axon guidance and cell migration
    Regulation of microtubule plus-end dynamics during axon guidance...
    A fundamental question in neuronal development is how growth cone cytoskeletal dynamics are coordinated to promote accurate axonal navigation. To address this question, we focus on microtubul...
    Speaker: Laura Lowery, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 13, 2017 | 1:30 PM
    Integration of the Simplexa C. difficile Direct assay in a two-step algorithm for the laboratory diagnosis of C. difficile
    Integration of the Simplexa C. difficile Direct assay in a two-step...
    Clostridium difficile is a major pathogen responsible for infectious diarrhea in healthcare settings. This presentation will review the data from a study comparing the new Simplexa® C. di...
    Speaker: Dr. James Snyder
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    Sponsored By: Diasorin
    2
     
     
  • MAY 11, 2017 | 12:00 PM
    New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology
    New multiplexed nCounter® PlexSet™ Reagents- an alternative to...
    The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme...
    Speaker: Christian Lytle
    Presented at: Genetics and Genomics Virtual Event Series 2017
    Sponsored By: NanoString Technologies
    1
    NOV 08, 2016 | 10:00 AM
    The genetics of aquaculture: Genotyping with GBS and arrays for species identification and trait improvement
    The genetics of aquaculture: Genotyping with GBS and arrays for...
    DATE: November 8, 2016 TIME: 10:00am PT, 1:00pm ET Stock and individual identification of Coho and Chinook Salmon in British Columbia via amplicon sequencing We have assemble...
    Speaker: Terry Beacham, PhD , Yniv Palti, PhD
    Sponsored By: Affymetrix, part of Thermo Fisher Scientific, Affymetrix, part of Thermo Fisher Scientific
    5 1
    NOV 02, 2016 | 10:30 AM
    Tough Targets. Simple Genotyping. Using HRM as a direct approach in research applications.
    Tough Targets. Simple Genotyping. Using HRM as a direct approach...
    Are there DNA targets in your lab that are difficult to genotype using standard PCR or sequencing?Do you ever need to genotype a single sample, and you don’t want to wait to batch sam...
    Speaker: Brian McNally, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    2
    OCT 01, 2015 | 12:00 PM
    Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR
    Detection and sequencing of rare mutations using mutation enrichment...
    Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challengin...
    Speaker: Mike Makrigiorgos, PhD, DABMP
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    2
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