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    Immunofluorescence (IF)

    Immunofluorescence (IF) is a type of immunohistochemistry technique that utilizes fluorophores to visualize various cellular antigens such as proteins. It uses antibodies and fluorescent detection to study the localization, relative expression, and activation states of target proteins in fixed cells or tissues.

    Webinars

    • November 12, 2020 9:00 AM PST | 4:00 PM GMT
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
      Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
      Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
      Speaker: Srabani Bhaumik, PhD
      Presented at: DISCOVERY Global User Symposium
      1
      November 12, 2020 6:00 AM PST | 1:00 PM GMT
      Chromogenic multiplex IHC; the future of immunohistochemistry for the practicing pathologist and for research
      Chromogenic multiplex IHC; the future of immunohistochemistry...
      The development of many different chromogens for light microscopy gives us the opportunity to explore different usage of multiplex immunohistochemistry (IHC) for clinical diagnostic purposes...
      Speaker: Charalambos C. Solomides, MD
      Presented at: DISCOVERY Global User Symposium
      1
      SEP 15, 2020 | 8:00 AM
      Next generation ultra high content analysis of the tumor immune microenvironment using MICS technology
      Next generation ultra high content analysis of the tumor immune...
      DATE: September 15, 2020 TIME: 08:00am PT Tumorigenesis and cancer progression are affected by the tumor immune microenvironment (TIME). The TIME not only influences the efficiency of existi...
      Speaker: Stefan Eulitz
      Sponsored By: Miltenyi Biotec
      15 2
      JUN 03, 2020 | 4:00 PM
      Microfluidic precision autostainer for fast multiplexed TSA-based immunofluorescence
      Microfluidic precision autostainer for fast multiplexed TSA-based...
      Multiplex immunofluorescence IHC is a powerful technique to address the increasing research needs in immuno-oncology to better understand the tumor microenvironment. In this webinar, we will...
      Speaker: Alexandre Kehren, M.Sc.
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: Lunaphore
      2
      JUN 03, 2020 | 12:00 PM
      How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
      How multiplexed imaging reveals key insights into the tumor microenvironment...
      Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understanding of cells and their surroundings by providin...
      Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
      Presented at: Immuno-Oncology Virtual Event Series 2020
      Sponsored By: Cytiva
      0
      MAY 27, 2020 | 8:00 AM
      How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
      How multiplexed imaging reveals key insights into the tumor microenvironment...
      DATE: May 27, 2020 TIME: 8:00am PT, 11:00am ET Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understand...
      Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
      Sponsored By: Cytiva
      18 2
      MAY 20, 2020 | 7:30 AM
      Keynote Presentation: Bridging Multiscale Physiological Systems via Microfluidics- from single cell to tissue-scale to organ-scale
      Keynote Presentation: Bridging Multiscale Physiological Systems...
      Precision medicine is the paradigm to develop treatments for patients based on molecular-targets that are effective in vivo when administered. In addition to identifying the molecular and ce...
      Speaker: Abraham Lee
      Presented at: Laboratory Automation & Informatics Virtual Event Series 2020
      1
      MAY 19, 2020 | 11:45 AM
      Single-nucleus and Spatial Transcriptomics of Archival Pancreatic Ductal Adenocarcinoma after Neoadjuvant Treatment
      Single-nucleus and Spatial Transcriptomics of Archival Pancreatic...
      Pancreatic ductal adenocarcinoma (PDAC) remains a treatment-refractory disease and existing molecular subtypes do not inform clinical decisions. Previously-identified bulk transcriptomic sub...
      Speaker: William L. Hwang, MD, PhD
      Presented at: Advancing Science - A Virtual Oncology Conference
      1
      MAR 17, 2020 | 8:00 AM
      Multiplexing on Mouse tissue using Cell Signaling Technology antibodies and OPAL IHC method
      Multiplexing on Mouse tissue using Cell Signaling Technology antibodies...
      DATE: March 17, 2020 TIME: 8:00am PT, 11:00am PT Cell Signaling Technology: The determination of target specificity in immunohistochemical analysis requires multiple validation steps. This w...
      Speaker: Bill Kennedy , Christopher Grange
      Sponsored By: Akoya Biosciences, Cell Signaling Technology
      9
      NOV 07, 2019 | 10:00 AM
      Akoya Biosciences Webinar Series: Validating Multiplexed Antibody Panels for Pancreatic Research Applications
      Akoya Biosciences Webinar Series: Validating Multiplexed Antibody...
      DATE: November 7, 2019TIME: 10:00am PST, 1:00pm EST Studying the pathogenesis of diabetes requires detailed analysis of the pancreatic islet microenvironment and its numerous c...
      Speaker: Diane Saunders, PhD , Grady Carlson, PhD
      Sponsored By: Akoya Biosciences
      5
      SEP 19, 2019 | 9:00 AM
      Automated Multiplex Immunofluorescence
      Automated Multiplex Immunofluorescence
      DATE: September 19, 2019TIME: 9:00am PT, 12:00pm ET Cancer research continues to push the boundaries with new advancements in tissue analysis and biomarker detection. Now, more...
      Speaker: Bethany Remeniuk, PhD , Traci DeGeer, BS, HT (ASCP) HTL, QIHC
      Sponsored By: Leica Biosystems
      6
      AUG 27, 2019 | 9:00 AM
      Akoya Biosciences Webinar Series:  Comprehensive Meta-analysis of Biomarker Technologies for Predictive Response of PD-1/PD-L1 Checkpoint Therapies
      Akoya Biosciences Webinar Series: Comprehensive Meta-analysis...
      DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
      Speaker: Cliff Hoyt, PhD , Steve Lu
      Sponsored By: Akoya Biosciences
      5
      DEC 06, 2018 | 7:00 AM
      Image-based screening for precision medicine and immunotherapy
      Image-based screening for precision medicine and immunotherapy
      DATE: December 06, 2018TIME:  7:00AM PSTHigh-throughput confocal microscopy has changed the way drug screening can be performed, by enabling the detection of single-cell pheno...
      Speaker: Dr. Gregory Vladimer
      Sponsored By: Revvity
      6
      NOV 08, 2018 | 9:00 AM
      Influenza 2018-2019: Are you ready?
      Influenza 2018-2019: Are you ready?
      DATE: November 8, 2018TIME:  9:00AM PDTAnnual epidemics of influenza place a substantial burden on the health of people in the United States. CDC estimates that influenza has...
      Speaker: Jay M. Lieberman, M.D.
      Sponsored By: Diasorin
      9
      OCT 03, 2018 | 9:00 AM
      Mechanisms of Myeloid Immunosuppression:  Functional Characterization of the Tumor Microenvironment Using Single and Multiplex IHC
      Mechanisms of Myeloid Immunosuppression: Functional Characterization...
      DATE:  October 3, 2018TIME:  9:00am PDT, 12:00pm EDT Infiltrating myeloid cells constitute a significant component of the suppressive tumor microenvironment (TME) a...
      Speaker: Christopher Grange
      Sponsored By: Cell Signaling Technology
      3
      APR 17, 2018 | 8:00 AM
      StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based Neural Spheroid Platform for  High Throughput Drug Screening
      StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based...
      DATE: April 17, 2018TIME: 8:00AM PST, 11:00AM ESTRecently, much research has focused on obtaining 3D brain organoids in an attempt to better recapitulate brain development and function...
      Speaker: Cassiano Carromeu, PhD , Grischa Chandy, PhD , Oksana Sirenko, PhD
      Sponsored By: Molecular Devices
      7 1
      APR 06, 2018 | 9:00 AM
      Multiplex Immunofluorescence Profiling of Tumor Biopsies Using UltraPlex mxIF Technology
      Multiplex Immunofluorescence Profiling of Tumor Biopsies Using...
      DATE: April 4, 2018TIME: 09:00am PDT, 12:00pm CEST Multiplex is the ability to characterize three or more biomarkers on a single tissue section. The technique enables the combi...
      Speaker: Helen Snyder, PhD , Marie-Louise Loupart, PhD
      Sponsored By: Leica Biosystems
      8
      OCT 12, 2017 | 9:00 AM
      Profiling the cell surface of cancer cell lines for biomarker/drug target discovery using MS-based proteomics
      Profiling the cell surface of cancer cell lines for biomarker/drug...
      In order to expand the treatment options of cancers driven by oncogenic RAS, new cell surface targets need to be identified and characterized. Here, we describe mass spectrometry based phenot...
      Speaker: Josip Blonder, MD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
      3
      JUL 01, 2017 | 12:00 AM
      Comparison of the Teratoma assay and in vitro surrogate tests for assessment of pluripotency of human pluripotent stem cells
      Comparison of the Teratoma assay and in vitro surrogate tests...
      For optimal use of human induced pluripotent stem cells (hiPSCs) it is essential to identify lines that are fully reprogrammed and of high quality with proven pluripotency in terms of differe...
      Speaker: Christian Freund
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      2
      MAY 30, 2017 | 9:00 AM
      Protein and Cell Analysis Education Series featuring the Antibody Validation Forum
      Protein and Cell Analysis Education Series featuring the Antibody...
      Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders  Reproducibility and antibody validation standards are two significant challenges f...
      Speaker: Aled Edwards, PhD , Matt Baker , Anita Bandrowski, PhD , Paul Wallace, PhD , John Rogers, PhD , Christoph Hergersberg, PhD
      Presented at: Protein and Cell Analysis Education Series
      15
      OCT 29, 2014 | 3:00 PM
      Drug-tailored, efficacy-predictive and prognostic biomarker approaches for proper patient selection
      Drug-tailored, efficacy-predictive and prognostic biomarker approaches...
      Over the last decade we have witnessed tremendous advances in our understanding of the underlying molecular alterations in human cancer. This has stimulated excitement for our ability to deve...
      Speaker: Peter Blume-Jensen, MD, PhD
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
      0
    November 12, 2020 9:00 AM PST | 4:00 PM GMT
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated stainer and reagents: assay optimization, imaging and future implications
    Fluorescent multiplexing of 3D spheroid FFPE with Roche automated...
    Three-dimensional (3D) cell culture spheroids and aggregates are preferred over monolayer cell culture due to their architectural and functional similarity to solid tumors. To study expressi...
    Speaker: Srabani Bhaumik, PhD
    Presented at: DISCOVERY Global User Symposium
    1
    November 12, 2020 6:00 AM PST | 1:00 PM GMT
    Chromogenic multiplex IHC; the future of immunohistochemistry for the practicing pathologist and for research
    Chromogenic multiplex IHC; the future of immunohistochemistry...
    The development of many different chromogens for light microscopy gives us the opportunity to explore different usage of multiplex immunohistochemistry (IHC) for clinical diagnostic purposes...
    Speaker: Charalambos C. Solomides, MD
    Presented at: DISCOVERY Global User Symposium
    1
    SEP 15, 2020 | 8:00 AM
    Next generation ultra high content analysis of the tumor immune microenvironment using MICS technology
    Next generation ultra high content analysis of the tumor immune...
    DATE: September 15, 2020 TIME: 08:00am PT Tumorigenesis and cancer progression are affected by the tumor immune microenvironment (TIME). The TIME not only influences the efficiency of existi...
    Speaker: Stefan Eulitz
    Sponsored By: Miltenyi Biotec
    15 2
    JUN 03, 2020 | 4:00 PM
    Microfluidic precision autostainer for fast multiplexed TSA-based immunofluorescence
    Microfluidic precision autostainer for fast multiplexed TSA-based...
    Multiplex immunofluorescence IHC is a powerful technique to address the increasing research needs in immuno-oncology to better understand the tumor microenvironment. In this webinar, we will...
    Speaker: Alexandre Kehren, M.Sc.
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: Lunaphore
    2
    JUN 03, 2020 | 12:00 PM
    How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
    How multiplexed imaging reveals key insights into the tumor microenvironment...
    Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understanding of cells and their surroundings by providin...
    Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
    Presented at: Immuno-Oncology Virtual Event Series 2020
    Sponsored By: Cytiva
    0
     
     
  • MAY 27, 2020 | 8:00 AM
    How multiplexed imaging reveals key insights into the tumor microenvironment to inform treatment and improve outcomes
    How multiplexed imaging reveals key insights into the tumor microenvironment...
    DATE: May 27, 2020 TIME: 8:00am PT, 11:00am ET Multiplexed immunofluorescent imaging sheds new light on cancer and other complex conditions. This imaging technique offers a deeper understand...
    Speaker: Paul Goodwin , Fiona Ginty , Prachi Bogetto
    Sponsored By: Cytiva
    18 2
    MAY 20, 2020 | 7:30 AM
    Keynote Presentation: Bridging Multiscale Physiological Systems via Microfluidics- from single cell to tissue-scale to organ-scale
    Keynote Presentation: Bridging Multiscale Physiological Systems...
    Precision medicine is the paradigm to develop treatments for patients based on molecular-targets that are effective in vivo when administered. In addition to identifying the molecular and ce...
    Speaker: Abraham Lee
    Presented at: Laboratory Automation & Informatics Virtual Event Series 2020
    1
    MAY 19, 2020 | 11:45 AM
    Single-nucleus and Spatial Transcriptomics of Archival Pancreatic Ductal Adenocarcinoma after Neoadjuvant Treatment
    Single-nucleus and Spatial Transcriptomics of Archival Pancreatic...
    Pancreatic ductal adenocarcinoma (PDAC) remains a treatment-refractory disease and existing molecular subtypes do not inform clinical decisions. Previously-identified bulk transcriptomic sub...
    Speaker: William L. Hwang, MD, PhD
    Presented at: Advancing Science - A Virtual Oncology Conference
    1
    MAR 17, 2020 | 8:00 AM
    Multiplexing on Mouse tissue using Cell Signaling Technology antibodies and OPAL IHC method
    Multiplexing on Mouse tissue using Cell Signaling Technology antibodies...
    DATE: March 17, 2020 TIME: 8:00am PT, 11:00am PT Cell Signaling Technology: The determination of target specificity in immunohistochemical analysis requires multiple validation steps. This w...
    Speaker: Bill Kennedy , Christopher Grange
    Sponsored By: Akoya Biosciences, Cell Signaling Technology
    9
    NOV 07, 2019 | 10:00 AM
    Akoya Biosciences Webinar Series: Validating Multiplexed Antibody Panels for Pancreatic Research Applications
    Akoya Biosciences Webinar Series: Validating Multiplexed Antibody...
    DATE: November 7, 2019TIME: 10:00am PST, 1:00pm EST Studying the pathogenesis of diabetes requires detailed analysis of the pancreatic islet microenvironment and its numerous c...
    Speaker: Diane Saunders, PhD , Grady Carlson, PhD
    Sponsored By: Akoya Biosciences
    5
     
     
  • SEP 19, 2019 | 9:00 AM
    Automated Multiplex Immunofluorescence
    Automated Multiplex Immunofluorescence
    DATE: September 19, 2019TIME: 9:00am PT, 12:00pm ET Cancer research continues to push the boundaries with new advancements in tissue analysis and biomarker detection. Now, more...
    Speaker: Bethany Remeniuk, PhD , Traci DeGeer, BS, HT (ASCP) HTL, QIHC
    Sponsored By: Leica Biosystems
    6
    AUG 27, 2019 | 9:00 AM
    Akoya Biosciences Webinar Series:  Comprehensive Meta-analysis of Biomarker Technologies for Predictive Response of PD-1/PD-L1 Checkpoint Therapies
    Akoya Biosciences Webinar Series: Comprehensive Meta-analysis...
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    Speaker: Cliff Hoyt, PhD , Steve Lu
    Sponsored By: Akoya Biosciences
    5
    DEC 06, 2018 | 7:00 AM
    Image-based screening for precision medicine and immunotherapy
    Image-based screening for precision medicine and immunotherapy
    DATE: December 06, 2018TIME:  7:00AM PSTHigh-throughput confocal microscopy has changed the way drug screening can be performed, by enabling the detection of single-cell pheno...
    Speaker: Dr. Gregory Vladimer
    Sponsored By: Revvity
    6
    NOV 08, 2018 | 9:00 AM
    Influenza 2018-2019: Are you ready?
    Influenza 2018-2019: Are you ready?
    DATE: November 8, 2018TIME:  9:00AM PDTAnnual epidemics of influenza place a substantial burden on the health of people in the United States. CDC estimates that influenza has...
    Speaker: Jay M. Lieberman, M.D.
    Sponsored By: Diasorin
    9
    OCT 03, 2018 | 9:00 AM
    Mechanisms of Myeloid Immunosuppression:  Functional Characterization of the Tumor Microenvironment Using Single and Multiplex IHC
    Mechanisms of Myeloid Immunosuppression: Functional Characterization...
    DATE:  October 3, 2018TIME:  9:00am PDT, 12:00pm EDT Infiltrating myeloid cells constitute a significant component of the suppressive tumor microenvironment (TME) a...
    Speaker: Christopher Grange
    Sponsored By: Cell Signaling Technology
    3
     
     
  • APR 17, 2018 | 8:00 AM
    StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based Neural Spheroid Platform for  High Throughput Drug Screening
    StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based...
    DATE: April 17, 2018TIME: 8:00AM PST, 11:00AM ESTRecently, much research has focused on obtaining 3D brain organoids in an attempt to better recapitulate brain development and function...
    Speaker: Cassiano Carromeu, PhD , Grischa Chandy, PhD , Oksana Sirenko, PhD
    Sponsored By: Molecular Devices
    7 1
    APR 06, 2018 | 9:00 AM
    Multiplex Immunofluorescence Profiling of Tumor Biopsies Using UltraPlex mxIF Technology
    Multiplex Immunofluorescence Profiling of Tumor Biopsies Using...
    DATE: April 4, 2018TIME: 09:00am PDT, 12:00pm CEST Multiplex is the ability to characterize three or more biomarkers on a single tissue section. The technique enables the combi...
    Speaker: Helen Snyder, PhD , Marie-Louise Loupart, PhD
    Sponsored By: Leica Biosystems
    8
    OCT 12, 2017 | 9:00 AM
    Profiling the cell surface of cancer cell lines for biomarker/drug target discovery using MS-based proteomics
    Profiling the cell surface of cancer cell lines for biomarker/drug...
    In order to expand the treatment options of cancers driven by oncogenic RAS, new cell surface targets need to be identified and characterized. Here, we describe mass spectrometry based phenot...
    Speaker: Josip Blonder, MD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
    3
    JUL 01, 2017 | 12:00 AM
    Comparison of the Teratoma assay and in vitro surrogate tests for assessment of pluripotency of human pluripotent stem cells
    Comparison of the Teratoma assay and in vitro surrogate tests...
    For optimal use of human induced pluripotent stem cells (hiPSCs) it is essential to identify lines that are fully reprogrammed and of high quality with proven pluripotency in terms of differe...
    Speaker: Christian Freund
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    2
    MAY 30, 2017 | 9:00 AM
    Protein and Cell Analysis Education Series featuring the Antibody Validation Forum
    Protein and Cell Analysis Education Series featuring the Antibody...
    Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders  Reproducibility and antibody validation standards are two significant challenges f...
    Speaker: Aled Edwards, PhD , Matt Baker , Anita Bandrowski, PhD , Paul Wallace, PhD , John Rogers, PhD , Christoph Hergersberg, PhD
    Presented at: Protein and Cell Analysis Education Series
    15
     
     
  • OCT 29, 2014 | 3:00 PM
    Drug-tailored, efficacy-predictive and prognostic biomarker approaches for proper patient selection
    Drug-tailored, efficacy-predictive and prognostic biomarker approaches...
    Over the last decade we have witnessed tremendous advances in our understanding of the underlying molecular alterations in human cancer. This has stimulated excitement for our ability to deve...
    Speaker: Peter Blume-Jensen, MD, PhD
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
    0
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