CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats. The name was minted at a time when the origin and use of the interspacing subsequences were not known. At that time the CRISPRs were described as segments of prokaryotic DNA containing short, repetitive base sequences. In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated) genes are located next to CRISPR sequences.
The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
EVENT DETAILS:DATE: May 16, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science our first commit...
Do you have targets that are difficult to genotype? Are you starting new projects and looking for a fast convenient genotyping solution?Whether screening CRISPR clones, exploring pharmaco...
Developing therapies for human diseases continues to face obstacles, particularly in translating targets or compounds identified by in vitro screening campaigns to valid targets or efficaciou...
CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availabilit...
The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...
DATE: February 8, 2017TIME: 1:30pm PT, 4:30pm ETThe powerful and expanding use of genetically modified mice introduces important aspects of genetics in managing these strains for breedi...
DATE: December 13, 2016TIME: 8:00 AM PT, 11:00 AM ETUnderstanding physiological mechanisms regulating growth and nutrient deposition in rainbow trout is central to establish production...
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. In early 2016 we developed a pipe...
Stem cells, specifically induced pluripotent stem cells (iPSCs), offer exciting potential for the future of cell therapy and regenerative medicine. More recently, genetic engineering of stem ...
The isolation of human embryonic stem cells (hESCs) and the discovery of human induced pluripotent stem cell (hiPSC) reprogramming have sparked a renaissance in stem cell biology, in vitro di...
Over the last 15 years, human pluripotent stem cell (hPSC) technologies have progressed from academic curiosities into tools with the promise to underpin commerce, leading to real progress in...
Antibodies are tremendously useful and versatile tools and they have found use in many areas of life science research. However, antibodies can be difficult to make and are used in inherently...
High content imaging and analysis offers a robust, high throughput method for analyzing large numbers of cells with the benefit of the spatial and temporal demarcation afforded by fluorescenc...
DATE: June 23, 2016
TIME: 8am Pacific time, 11am Eastern time
In this webinar you will learn:
Key factors in successful arrayed crRNA screening
Benefits of the dual CR...
DATE: June 22, 2016
TIME: 9am Pacific time, 12pm Eastern time, 6pm Central European time
A large fraction of the RNA transcribed in eukaryotic cells is rapidly degraded in the nucleus. A ...
DNA methylation is an essential mechanism of epigenetic gene regulation with broad relevance in development and disease. Its localization on genomic DNA and general stability make this epigen...
DATE: March 31, 2016
TIME: 8am PT, 11am ET, 4pm GMT
In this webinar, you will learn:
Basics of homology-directed repair (HDR) using CRISPR-Cas9
Selection of CRISP...
DATE: January 21, 2016
TIME: 8am Pacific time, 11am Eastern time
In this webinar, you will learn:
basics of pooled lentiviral screening workflows
implications of ...
To learn more about Gene Editing watch “Webinar: CRISPR-Cas9 Gene Editing with Synthetic RNA – from start to finish!”
Attendees will learn:
the importance of ...
Genome editing technologies, including the CRISPR/Cas9 system, allow for precise and corrective molecular modifications to treat the underlying cause of genetic diseases. Editas Medicine is d...
The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
EVENT DETAILS:DATE: May 16, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science our first commit...
Do you have targets that are difficult to genotype? Are you starting new projects and looking for a fast convenient genotyping solution?Whether screening CRISPR clones, exploring pharmaco...
Developing therapies for human diseases continues to face obstacles, particularly in translating targets or compounds identified by in vitro screening campaigns to valid targets or efficaciou...
CRISPR-Cas9 has increased the accessibility of genome engineering due to its ease of use and ability to cause double strand breaks (DSBs) at almost any locus of interest. DSBs are repaired in...
There is rapidly growing interest in using the CRISPR-Cas9 system for functional screening, both as a primary screening tool and as an orthogonal tool for RNAi hit validation. High throughput...
The use of pluripotent stem cell-derived cell types for disease modeling, drug screening and regenerative medicine is an exciting area of activity in health research. Prior to the availabilit...
The CRISPR-Cas9 system is being widely used for genome engineering in many different biological applications. It was originally adapted from the bacterial Type II CRISPR system and uses a Cas...
This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway wil...
DATE: February 8, 2017TIME: 1:30pm PT, 4:30pm ETThe powerful and expanding use of genetically modified mice introduces important aspects of genetics in managing these strains for breedi...
DATE: December 13, 2016TIME: 8:00 AM PT, 11:00 AM ETUnderstanding physiological mechanisms regulating growth and nutrient deposition in rainbow trout is central to establish production...
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. In early 2016 we developed a pipe...
Stem cells, specifically induced pluripotent stem cells (iPSCs), offer exciting potential for the future of cell therapy and regenerative medicine. More recently, genetic engineering of stem ...
The isolation of human embryonic stem cells (hESCs) and the discovery of human induced pluripotent stem cell (hiPSC) reprogramming have sparked a renaissance in stem cell biology, in vitro di...
Over the last 15 years, human pluripotent stem cell (hPSC) technologies have progressed from academic curiosities into tools with the promise to underpin commerce, leading to real progress in...
Antibodies are tremendously useful and versatile tools and they have found use in many areas of life science research. However, antibodies can be difficult to make and are used in inherently...
High content imaging and analysis offers a robust, high throughput method for analyzing large numbers of cells with the benefit of the spatial and temporal demarcation afforded by fluorescenc...
DATE: June 23, 2016
TIME: 8am Pacific time, 11am Eastern time
In this webinar you will learn:
Key factors in successful arrayed crRNA screening
Benefits of the dual CR...
DATE: June 22, 2016
TIME: 9am Pacific time, 12pm Eastern time, 6pm Central European time
A large fraction of the RNA transcribed in eukaryotic cells is rapidly degraded in the nucleus. A ...
DNA methylation is an essential mechanism of epigenetic gene regulation with broad relevance in development and disease. Its localization on genomic DNA and general stability make this epigen...
DATE: March 31, 2016
TIME: 8am PT, 11am ET, 4pm GMT
In this webinar, you will learn:
Basics of homology-directed repair (HDR) using CRISPR-Cas9
Selection of CRISP...
DATE: January 21, 2016
TIME: 8am Pacific time, 11am Eastern time
In this webinar, you will learn:
basics of pooled lentiviral screening workflows
implications of ...
To learn more about Gene Editing watch “Webinar: CRISPR-Cas9 Gene Editing with Synthetic RNA – from start to finish!”
Attendees will learn:
the importance of ...
Genome editing technologies, including the CRISPR/Cas9 system, allow for precise and corrective molecular modifications to treat the underlying cause of genetic diseases. Editas Medicine is d...
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