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    DNA Isolation & Purification

    DNA Isolation & Purification: DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. The first isolation of DNA was done in 1869 by Friedrich Miescher. Currently it is a routine procedure in molecular biology or forensic analyses. The procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.

    Webinars

    • JUL 22, 2020 | 10:00 AM
      Molecular Diagnostic Solutions in a Pandemic: Planning for the 2020/2021 Flu Season with the Implementation of the ePlex® Respiratory Pathogen Panel 2
      Molecular Diagnostic Solutions in a Pandemic: Planning for the...
      DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
      Speaker: Heba Mostafa, MD, PhD, D(ABMM)
      Sponsored By: GenMark Dx
      27 13
      JAN 29, 2020 | 9:00 AM
      A case study of protein sample prep strategies for membrane proteins
      A case study of protein sample prep strategies for membrane proteins
      DATE: January 29, 2019 TIME: 9:00am PST Plasma membrane proteins are a difficult subset of proteins to solubilize, purify, quantify, and detect, largely due to the challenge of extracting th...
      Speaker: Barbara Kaboord, PhD
      Sponsored By: Thermo Fisher Scientific
      6
      DEC 04, 2019 | 7:00 AM
      Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
      Improve your reproducibility - Liquid Handling is a crucial factor...
      DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
      Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
      Sponsored By: Eppendorf
      12
      NOV 19, 2019 | 8:00 AM
      Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows
      Overcoming the Hurdles of Density Gradient Ultracentrifugation...
      DATE: November 19, 2019TIME: 8:00am PTCentrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of discipline...
      Speaker: Ross VerHeul
      Sponsored By: Beckman Coulter Life Sciences
      7 1
      NOV 18, 2019 | 7:00 AM
      Protect your cells with proper pipetting - How liquid handling influences your cell culture work
      Protect your cells with proper pipetting - How liquid handling...
      DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
      Speaker: Nadine Mellies, PhD , Katja Baesler, PhD
      Sponsored By: Eppendorf
      22 2
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
      JUN 05, 2019 | 5:00 PM
      Everyday culture practice - Improving reproducibility in cell culture
      Everyday culture practice - Improving reproducibility in cell...
      DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
      Speaker: Jessica Wagener, PhD
      Sponsored By: Eppendorf
      23 2
      MAY 16, 2019 | 4:00 PM
      Towards Standardized Workflows - A focus on microbiome insights
      Towards Standardized Workflows - A focus on microbiome insights
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
      Sponsored By: Eppendorf
      10 2
      OCT 03, 2018 | 12:00 PM
      Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
      Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
      All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
      Speaker: Dipali Sashital, PhD
      Presented at: CRISPR Virtual Event Series 2018
      3
      OCT 03, 2018 | 9:00 AM
      CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs in CRISPR-Cas9 Genome-Editing Systems
      CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs...
      The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...
      Speaker: Lihua Julie Zhu, PhD
      Presented at: CRISPR Virtual Event Series 2018
      3
      SEP 27, 2018 | 7:30 AM
      Keynote Presentation: Mapping the Inner World of the Cell
      Keynote Presentation: Mapping the Inner World of the Cell
      Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...
      Speaker: Bo Huang, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      1
      SEP 26, 2018 | 12:00 PM
      3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
      3D Cell Cultures and Tissue Clearing: A High Content Confocal...
      With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
      Speaker: Michael Johnson, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      2
      SEP 18, 2018 | 8:00 AM
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
      Speaker: Judd Hultquist, PhD
      Sponsored By: Horizon Discovery
      7
      MAY 03, 2018 | 11:00 AM
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
      DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
      Speaker: Sisi Li
      Sponsored By: Covaris
      5 2
      APR 24, 2018 | 8:00 AM
      KASP genotyping - better by design
      KASP genotyping - better by design
      DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
      Speaker: Jonathan Curry, PhD
      Sponsored By: LGC Seracare
      13
      MAR 27, 2018 | 9:00 AM
      How to use CRISPR-Cas9 for knockout, knock-in and gene activation
      How to use CRISPR-Cas9 for knockout, knock-in and gene activation
      DATE: March 27, 2018TIME: 09:00am PDT, 12:00pm EDTThe rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, a...
      Speaker: Amanda Haas
      Sponsored By: Horizon Discovery
      23 15
      MAR 22, 2018 | 7:00 AM
      Emerging Diagnostic Strategies to Support C. difficile Infection Control
      Emerging Diagnostic Strategies to Support C. difficile Infection...
      DATE: March 22, 2018TIME: 07:00am PDT, 10:00am EDTClostridium difficile (C. diff) is a highly problematic healthcare-associated infection that is easily spread and often results in poor...
      Speaker: Lance Peterson, MD, FASCP, FIDSA, FAAM, FSHEA
      Sponsored By: Roche Diagnostics
      8
      SEP 28, 2017 | 1:30 PM
      The RRID Initiative, what is needed for scholarship in the 21st century
      The RRID Initiative, what is needed for scholarship in the 21st...
      The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
      Speaker: Anita Bandrowski, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 12:00 PM
      Back to basics: Fundamental concepts and special considerations in RNA isolation
      Back to basics: Fundamental concepts and special considerations...
      How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
      Speaker: Abhishek Sharma
      Presented at: Cell Biology Virtual Event Series 2017
      2
      SEP 28, 2017 | 10:30 AM
      Engineering stem cell biology for disease modelling and therapeutics
      Engineering stem cell biology for disease modelling and therapeutics
      Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
      Speaker: Amr Abdeen, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 9:00 AM
      Fine tuning of myosin activity shapes actomyosin network organization and tissue contractility of the C. elegans spermatheca
      Fine tuning of myosin activity shapes actomyosin network organization...
      Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
      Speaker: Alison Wirshing, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 7:30 AM
      Keynote Presentation: RNA-targeting CRISPR and application to diseases
      Keynote Presentation: RNA-targeting CRISPR and application to...
      I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
      Speaker: Gene Yeo, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 6:00 AM
      The axolotl salamander as a model for nerve-dependent regeneration
      The axolotl salamander as a model for nerve-dependent regeneration
      The Mexican axolotl (Ambystoma mexicanum) is an aquatic salamander that possesses some of the most astonishing regenerative abilities found in nature.  Able to fully regenerate amputated...
      Speaker: Johanna Farkas, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 1:30 PM
      Evolutionary Cell Biology is a powerful paradigm to study complex biological systems
      Evolutionary Cell Biology is a powerful paradigm to study complex...
      Although the cell is commonly referred to as “the most basic unit of life”, it is actually so complex that despite over 350 years of research we are still far from fully understan...
      Speaker: Idan Frumkin, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
    JUL 22, 2020 | 10:00 AM
    Molecular Diagnostic Solutions in a Pandemic: Planning for the 2020/2021 Flu Season with the Implementation of the ePlex® Respiratory Pathogen Panel 2
    Molecular Diagnostic Solutions in a Pandemic: Planning for the...
    DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
    Speaker: Heba Mostafa, MD, PhD, D(ABMM)
    Sponsored By: GenMark Dx
    27 13
    JAN 29, 2020 | 9:00 AM
    A case study of protein sample prep strategies for membrane proteins
    A case study of protein sample prep strategies for membrane proteins
    DATE: January 29, 2019 TIME: 9:00am PST Plasma membrane proteins are a difficult subset of proteins to solubilize, purify, quantify, and detect, largely due to the challenge of extracting th...
    Speaker: Barbara Kaboord, PhD
    Sponsored By: Thermo Fisher Scientific
    6
    DEC 04, 2019 | 7:00 AM
    Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
    Improve your reproducibility - Liquid Handling is a crucial factor...
    DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
    Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
    Sponsored By: Eppendorf
    12
    NOV 19, 2019 | 8:00 AM
    Overcoming the Hurdles of Density Gradient Ultracentrifugation for Optimized Gene Therapy Purification Workflows
    Overcoming the Hurdles of Density Gradient Ultracentrifugation...
    DATE: November 19, 2019TIME: 8:00am PTCentrifugation has long served as a critical separation tool in countless research and production facilities spanning a wide range of discipline...
    Speaker: Ross VerHeul
    Sponsored By: Beckman Coulter Life Sciences
    7 1
    NOV 18, 2019 | 7:00 AM
    Protect your cells with proper pipetting - How liquid handling influences your cell culture work
    Protect your cells with proper pipetting - How liquid handling...
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    Speaker: Nadine Mellies, PhD , Katja Baesler, PhD
    Sponsored By: Eppendorf
    22 2
     
     
  • SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
    17 1
    JUN 05, 2019 | 5:00 PM
    Everyday culture practice - Improving reproducibility in cell culture
    Everyday culture practice - Improving reproducibility in cell...
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    Speaker: Jessica Wagener, PhD
    Sponsored By: Eppendorf
    23 2
    MAY 16, 2019 | 4:00 PM
    Towards Standardized Workflows - A focus on microbiome insights
    Towards Standardized Workflows - A focus on microbiome insights
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
    Sponsored By: Eppendorf
    10 2
    OCT 03, 2018 | 12:00 PM
    Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
    Mechanisms of CRISPR-Cas Systems: From Adaptive Immunity to Biotechnology
    All forms of life require immune systems to stave off infection from viruses and other pathogens. In bacteria and archaea, clustered regularly interspaced short palindromic repeats (CRISPR) a...
    Speaker: Dipali Sashital, PhD
    Presented at: CRISPR Virtual Event Series 2018
    3
    OCT 03, 2018 | 9:00 AM
    CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs in CRISPR-Cas9 Genome-Editing Systems
    CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs...
    The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...
    Speaker: Lihua Julie Zhu, PhD
    Presented at: CRISPR Virtual Event Series 2018
    3
     
     
  • SEP 27, 2018 | 7:30 AM
    Keynote Presentation: Mapping the Inner World of the Cell
    Keynote Presentation: Mapping the Inner World of the Cell
    Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...
    Speaker: Bo Huang, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    1
    SEP 26, 2018 | 12:00 PM
    3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
    3D Cell Cultures and Tissue Clearing: A High Content Confocal...
    With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
    Speaker: Michael Johnson, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    2
    SEP 18, 2018 | 8:00 AM
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
    Speaker: Judd Hultquist, PhD
    Sponsored By: Horizon Discovery
    7
    MAY 03, 2018 | 11:00 AM
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    Speaker: Sisi Li
    Sponsored By: Covaris
    5 2
    APR 24, 2018 | 8:00 AM
    KASP genotyping - better by design
    KASP genotyping - better by design
    DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
    Speaker: Jonathan Curry, PhD
    Sponsored By: LGC Seracare
    13
     
     
  • MAR 27, 2018 | 9:00 AM
    How to use CRISPR-Cas9 for knockout, knock-in and gene activation
    How to use CRISPR-Cas9 for knockout, knock-in and gene activation
    DATE: March 27, 2018TIME: 09:00am PDT, 12:00pm EDTThe rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, a...
    Speaker: Amanda Haas
    Sponsored By: Horizon Discovery
    23 15
    MAR 22, 2018 | 7:00 AM
    Emerging Diagnostic Strategies to Support C. difficile Infection Control
    Emerging Diagnostic Strategies to Support C. difficile Infection...
    DATE: March 22, 2018TIME: 07:00am PDT, 10:00am EDTClostridium difficile (C. diff) is a highly problematic healthcare-associated infection that is easily spread and often results in poor...
    Speaker: Lance Peterson, MD, FASCP, FIDSA, FAAM, FSHEA
    Sponsored By: Roche Diagnostics
    8
    SEP 28, 2017 | 1:30 PM
    The RRID Initiative, what is needed for scholarship in the 21st century
    The RRID Initiative, what is needed for scholarship in the 21st...
    The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
    Speaker: Anita Bandrowski, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 12:00 PM
    Back to basics: Fundamental concepts and special considerations in RNA isolation
    Back to basics: Fundamental concepts and special considerations...
    How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
    Speaker: Abhishek Sharma
    Presented at: Cell Biology Virtual Event Series 2017
    2
    SEP 28, 2017 | 10:30 AM
    Engineering stem cell biology for disease modelling and therapeutics
    Engineering stem cell biology for disease modelling and therapeutics
    Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
    Speaker: Amr Abdeen, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
     
     
  • SEP 28, 2017 | 9:00 AM
    Fine tuning of myosin activity shapes actomyosin network organization and tissue contractility of the C. elegans spermatheca
    Fine tuning of myosin activity shapes actomyosin network organization...
    Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
    Speaker: Alison Wirshing, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 7:30 AM
    Keynote Presentation: RNA-targeting CRISPR and application to diseases
    Keynote Presentation: RNA-targeting CRISPR and application to...
    I will present my lab’s effort on studying and manipulating RNA processing, with particular emphasis on using CRISPR/Cas systems for targeting RNA in living cells in diseases such as my...
    Speaker: Gene Yeo, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 6:00 AM
    The axolotl salamander as a model for nerve-dependent regeneration
    The axolotl salamander as a model for nerve-dependent regeneration
    The Mexican axolotl (Ambystoma mexicanum) is an aquatic salamander that possesses some of the most astonishing regenerative abilities found in nature.  Able to fully regenerate amputated...
    Speaker: Johanna Farkas, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 1:30 PM
    Evolutionary Cell Biology is a powerful paradigm to study complex biological systems
    Evolutionary Cell Biology is a powerful paradigm to study complex...
    Although the cell is commonly referred to as “the most basic unit of life”, it is actually so complex that despite over 350 years of research we are still far from fully understan...
    Speaker: Idan Frumkin, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
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