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    Qpcr

    qPCR: Real-time PCR and quantitative PCR (qPCR) Real-Time PCR refers to the fact that measurements are made during the amplification as opposed to at the end of PCR. qPCR introduces the idea that the data provides quantification of the target.

    Webinars

    • MAY 09, 2018 | 9:00 AM
      Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
      Gene Expression Analysis - Critical Factors for Overcoming Challenges...
      Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
      Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      MAY 03, 2018 | 11:00 AM
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
      DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
      Speaker: Sisi Li
      Sponsored By: Covaris
      5 2
      APR 24, 2018 | 8:00 AM
      KASP genotyping - better by design
      KASP genotyping - better by design
      DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
      Speaker: Jonathan Curry, PhD
      Sponsored By: LGC Seracare
      13
      OCT 12, 2017 | 12:00 PM
      Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
      Detection of circulating tumour biomarkers using the FirePlex®...
      The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
      Speaker: Daniel Pregibon, PhD
      Presented at: Abcam virtual exhibition: support and products to advance your research
      2
      OCT 12, 2017 | 12:00 PM
      Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
      Detection of circulating tumour biomarkers using the FirePlex®...
      The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
      Speaker: Daniel Pregibon, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
      Sponsored By: Abcam
      1
      SEP 06, 2017 | 9:00 AM
      WEBINAR: Manufacturing and characterization of therapeutic exosomes for clinical studies
      WEBINAR: Manufacturing and characterization of therapeutic exosomes...
      DATE: September 6, 2017TIME: 9:00AM PST, 12:00PM ET The era of using extracellular vesicles (EVs) and exosomes for regenerative medicine applications in the clinic is here. We...
      Speaker: Travis Antes, PhD
      Sponsored By: Miltenyi Biotec
      9
      JUL 01, 2017 | 12:00 AM
      Comprehensive Characterization of Pluripotent Stem Cells
      Comprehensive Characterization of Pluripotent Stem Cells
      As reprogramming methodologies have become more reliable and efficient, corresponding improvements were needed in the characterization workflow as well; specifically in terms of the speed, ef...
      Speaker: Suman Pradhan
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      1
      JUN 28, 2017 | 7:00 AM
      Analytical methods to measure empty and full AAV particles
      Analytical methods to measure empty and full AAV particles
      DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
      Speaker: Christine LeBec, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences
      9 2
      MAY 11, 2017 | 12:00 PM
      New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology
      New multiplexed nCounter® PlexSet™ Reagents- an alternative to...
      The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme...
      Speaker: Christian Lytle
      Presented at: Genetics and Genomics Virtual Event Series 2017
      Sponsored By: NanoString Technologies
      1
      APR 13, 2017 | 10:30 AM
      Why Variants Matter: NGS Bioinformatics Simplified for Oncology & Virology
      Why Variants Matter: NGS Bioinformatics Simplified for Oncology...
      Next-generation sequencing is expected to take molecular diagnostics to the next level with the capability to perform deep analysis of genetic information that leads to targeted therapies per...
      Speaker: Condie Carmack, PhD , Ahmed Mahmoud, PhD
      Presented at: Molecular Diagnostics Virtual Event Series 2017
      Sponsored By: Vela Diagnostics
      3
      FEB 23, 2017 | 6:00 AM
      Obtaining Samples for Precision Medicine Studies: An Outsider's Perspective
      Obtaining Samples for Precision Medicine Studies: An Outsider's...
      Best practices for developing precision medicine diagnostics can include completing a retrospective analysis.  Obtaining enough samples to have a statistically significant study typicall...
      Speaker: Matt Klusas
      Presented at: Precision Medicine Virtual Event Series 2017
      2
      NOV 03, 2016 | 6:00 AM
      Mutation Detection Simplified: From Liquid Biopsy in clinical oncology to the detection of Drug Resistance Associated Mutations in virology
      Mutation Detection Simplified: From Liquid Biopsy in clinical...
      Sanger sequencing and polymerase chain reaction (PCR) methods have been the standard molecular methods in clinical diagnostics for decades.  Next-Generation Sequencing (NGS) technology r...
      Speaker: Gerd Michel, PhD , Lou Welebob
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      5
      OCT 05, 2016 | 6:00 AM
      Unique molecular indices (UMI) and their application in detecting novel gene fusions and gene expression and genetic variation
      Unique molecular indices (UMI) and their application in detecting...
      Tumor heterogeneity has been known for a while but quantifying heterogeneity is still a challenge.  NGS is the method of choice in the analysis of tumor heterogeneity, however, there are...
      Speaker: Samuel Rulli, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      3
      NOV 12, 2015 | 1:30 PM
      NGS in clinical research
      NGS in clinical research
      Currently, next-generation sequencing is not only being used for basic discovery research but also in clinical settings for translational biomarker profiling and clinical research studies inc...
      Speaker: Jennifer Fostel, MS
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      1
      SEP 15, 2015 | 10:00 AM
      A new integrated platform for high quality and low cost gene expression analysis
      A new integrated platform for high quality and low cost gene expression...
      DATE: September 15, 2015TIME: 10:00AM Pacific time, 1:00PM Eastern timeThe breadth of instruments available for quantitative PCR (qPCR) has continued to grow in the past 5-10 years. With ...
      Speaker: Mikael Kubista , Ross Higgins
      Sponsored By: Douglas Scientific, Douglas Scientific
      4
      SEP 02, 2015 | 6:00 AM
      Rapid Tests to Decipher Microbiome Function in Health and Disease
      Rapid Tests to Decipher Microbiome Function in Health and Disease
      Human microbiome projects have delivered our first glimpse into the microbial communities that reside in and on our bodies. Building on these efforts the research community has begun to corre...
      Speaker: Anisha Kharkia, MS
      Presented at: Microbiology Virtual Event Series 2015
      10
      JUN 11, 2015 | 11:00 AM
      Assessing Quality Using ACCURUN® Controls for Quantitative Viral Load Assays
      Assessing Quality Using ACCURUN® Controls for Quantitative Viral...
      Consistent accuracy in reporting quantitative viral load results is critically important to patients, their physicians and to the clinical laboratory. In clinical practice, quantitative viral...
      Speaker: Russell Garlick
      Presented at: Cardinal Health labXchange
      Sponsored By: Cardinal Healthâ„¢ OptiFreight® Logistics
      6
      MAY 26, 2015 | 9:00 AM
      Intelligent NGS Assay Design: Oncomineâ„¢ Focus Assay for NGS clinical research in oncology
      Intelligent NGS Assay Design: Oncomineâ„¢ Focus Assay for NGS clinical...
      DATE: Tuesday, May 26th, 2015TIME: 09:00AM PDT, 12:00PM EDTNext-generation sequencing (NGS) is changing the landscape of clinical research. This powerful technology is enabling researchers to...
      Speaker: Seth Sadis
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      7
      JAN 27, 2015 | 2:00 AM
      Catch-and-Release: Extraction and Purification of NGS-Grade DNA and RNA from FFPE Tissue Samples
      Catch-and-Release: Extraction and Purification of NGS-Grade DNA...
      Formalin Fixed, Paraffin Embedded (FFPE) tissues are rapidly being adopted for transcriptome analysis in conjunction with targeted and whole genome sequencing. Harsh formaldehyde fixation an...
      Speaker: Guillaume Durin, PhD
      Sponsored By: Covaris, Inc., Covaris, Inc.
      10
      AUG 21, 2013 | 2:00 PM
      C.E. CREDITS
      Comprehensive Chromosome Screening in the Human Embryo
      Comprehensive Chromosome Screening in the Human Embryo
      The human species is particularly prone to chromosome segregation errors during maternal meiosis in the egg and during post zygotic mitosis in the preimplantation embryo. In fact, aneuploidy...
      Speaker: Nathan R Treff, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2013
      33
      AUG 21, 2013 | 11:00 AM
      Panel Discussion: Meeting the Demand of Increased NGS Capacity with Automated TruSeq Stranded mRNA Library Preparation
      Panel Discussion: Meeting the Demand of Increased NGS Capacity...
      RNASeq has become the primary method for studying changes in gene expression in modern genomics research. By directly sequencing the various types of RNA molecules, including mRNA, ncRNA, an...
      Speaker: Alisa Jackson, Zach Smith, Mary Blair, Dave Delano
      Presented at: Genetics and Genomics Virtual Event Series 2013
      41
      MAY 30, 2013 | 12:00 PM
      Companion Diagnostics Co-Development: Are you ready?
      Companion Diagnostics Co-Development: Are you ready?
      Development of a companion diagnostic can be critical to the success of a drug. Pharmaceutical companies are committing more and more of their efforts to discover and deliver targeted therapi...
      Speaker: Janice Au-Young, PhD
      Presented at: Clinical Diagnostics Virtual Event Series 2013
      15
    MAY 09, 2018 | 9:00 AM
    Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
    Gene Expression Analysis - Critical Factors for Overcoming Challenges...
    Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
    Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
    MAY 03, 2018 | 11:00 AM
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    Speaker: Sisi Li
    Sponsored By: Covaris
    5 2
    APR 24, 2018 | 8:00 AM
    KASP genotyping - better by design
    KASP genotyping - better by design
    DATE: April 24, 2018TIME: 8:00AM PSTIn this talk we will cover basic principles of KASP® genotyping chemistry and designing assays that are specific to desired targets. We will go o...
    Speaker: Jonathan Curry, PhD
    Sponsored By: LGC Seracare
    13
    OCT 12, 2017 | 12:00 PM
    Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
    Detection of circulating tumour biomarkers using the FirePlex®...
    The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
    Speaker: Daniel Pregibon, PhD
    Presented at: Abcam virtual exhibition: support and products to advance your research
    2
    OCT 12, 2017 | 12:00 PM
    Detection of circulating tumour biomarkers using the FirePlex® Technology Platform
    Detection of circulating tumour biomarkers using the FirePlex®...
    The detection of circulating molecular biomarkers is useful for monitoring pathogenic processes and response to therapeutic intervention1,2. In cancer, genomic and proteomic biomarkers are us...
    Speaker: Daniel Pregibon, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
    Sponsored By: Abcam
    1
     
     
  • SEP 06, 2017 | 9:00 AM
    WEBINAR: Manufacturing and characterization of therapeutic exosomes for clinical studies
    WEBINAR: Manufacturing and characterization of therapeutic exosomes...
    DATE: September 6, 2017TIME: 9:00AM PST, 12:00PM ET The era of using extracellular vesicles (EVs) and exosomes for regenerative medicine applications in the clinic is here. We...
    Speaker: Travis Antes, PhD
    Sponsored By: Miltenyi Biotec
    9
    JUL 01, 2017 | 12:00 AM
    Comprehensive Characterization of Pluripotent Stem Cells
    Comprehensive Characterization of Pluripotent Stem Cells
    As reprogramming methodologies have become more reliable and efficient, corresponding improvements were needed in the characterization workflow as well; specifically in terms of the speed, ef...
    Speaker: Suman Pradhan
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    1
    JUN 28, 2017 | 7:00 AM
    Analytical methods to measure empty and full AAV particles
    Analytical methods to measure empty and full AAV particles
    DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
    Speaker: Christine LeBec, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences
    9 2
    MAY 11, 2017 | 12:00 PM
    New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology
    New multiplexed nCounter® PlexSet™ Reagents- an alternative to...
    The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme...
    Speaker: Christian Lytle
    Presented at: Genetics and Genomics Virtual Event Series 2017
    Sponsored By: NanoString Technologies
    1
    APR 13, 2017 | 10:30 AM
    Why Variants Matter: NGS Bioinformatics Simplified for Oncology & Virology
    Why Variants Matter: NGS Bioinformatics Simplified for Oncology...
    Next-generation sequencing is expected to take molecular diagnostics to the next level with the capability to perform deep analysis of genetic information that leads to targeted therapies per...
    Speaker: Condie Carmack, PhD , Ahmed Mahmoud, PhD
    Presented at: Molecular Diagnostics Virtual Event Series 2017
    Sponsored By: Vela Diagnostics
    3
     
     
  • FEB 23, 2017 | 6:00 AM
    Obtaining Samples for Precision Medicine Studies: An Outsider's Perspective
    Obtaining Samples for Precision Medicine Studies: An Outsider's...
    Best practices for developing precision medicine diagnostics can include completing a retrospective analysis.  Obtaining enough samples to have a statistically significant study typicall...
    Speaker: Matt Klusas
    Presented at: Precision Medicine Virtual Event Series 2017
    2
    NOV 03, 2016 | 6:00 AM
    Mutation Detection Simplified: From Liquid Biopsy in clinical oncology to the detection of Drug Resistance Associated Mutations in virology
    Mutation Detection Simplified: From Liquid Biopsy in clinical...
    Sanger sequencing and polymerase chain reaction (PCR) methods have been the standard molecular methods in clinical diagnostics for decades.  Next-Generation Sequencing (NGS) technology r...
    Speaker: Gerd Michel, PhD , Lou Welebob
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    5
    OCT 05, 2016 | 6:00 AM
    Unique molecular indices (UMI) and their application in detecting novel gene fusions and gene expression and genetic variation
    Unique molecular indices (UMI) and their application in detecting...
    Tumor heterogeneity has been known for a while but quantifying heterogeneity is still a challenge.  NGS is the method of choice in the analysis of tumor heterogeneity, however, there are...
    Speaker: Samuel Rulli, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    3
    NOV 12, 2015 | 1:30 PM
    NGS in clinical research
    NGS in clinical research
    Currently, next-generation sequencing is not only being used for basic discovery research but also in clinical settings for translational biomarker profiling and clinical research studies inc...
    Speaker: Jennifer Fostel, MS
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    1
    SEP 15, 2015 | 10:00 AM
    A new integrated platform for high quality and low cost gene expression analysis
    A new integrated platform for high quality and low cost gene expression...
    DATE: September 15, 2015TIME: 10:00AM Pacific time, 1:00PM Eastern timeThe breadth of instruments available for quantitative PCR (qPCR) has continued to grow in the past 5-10 years. With ...
    Speaker: Mikael Kubista , Ross Higgins
    Sponsored By: Douglas Scientific, Douglas Scientific
    4
     
     
  • SEP 02, 2015 | 6:00 AM
    Rapid Tests to Decipher Microbiome Function in Health and Disease
    Rapid Tests to Decipher Microbiome Function in Health and Disease
    Human microbiome projects have delivered our first glimpse into the microbial communities that reside in and on our bodies. Building on these efforts the research community has begun to corre...
    Speaker: Anisha Kharkia, MS
    Presented at: Microbiology Virtual Event Series 2015
    10
    JUN 11, 2015 | 11:00 AM
    Assessing Quality Using ACCURUN® Controls for Quantitative Viral Load Assays
    Assessing Quality Using ACCURUN® Controls for Quantitative Viral...
    Consistent accuracy in reporting quantitative viral load results is critically important to patients, their physicians and to the clinical laboratory. In clinical practice, quantitative viral...
    Speaker: Russell Garlick
    Presented at: Cardinal Health labXchange
    Sponsored By: Cardinal Healthâ„¢ OptiFreight® Logistics
    6
    MAY 26, 2015 | 9:00 AM
    Intelligent NGS Assay Design: Oncomineâ„¢ Focus Assay for NGS clinical research in oncology
    Intelligent NGS Assay Design: Oncomineâ„¢ Focus Assay for NGS clinical...
    DATE: Tuesday, May 26th, 2015TIME: 09:00AM PDT, 12:00PM EDTNext-generation sequencing (NGS) is changing the landscape of clinical research. This powerful technology is enabling researchers to...
    Speaker: Seth Sadis
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    7
    JAN 27, 2015 | 2:00 AM
    Catch-and-Release: Extraction and Purification of NGS-Grade DNA and RNA from FFPE Tissue Samples
    Catch-and-Release: Extraction and Purification of NGS-Grade DNA...
    Formalin Fixed, Paraffin Embedded (FFPE) tissues are rapidly being adopted for transcriptome analysis in conjunction with targeted and whole genome sequencing. Harsh formaldehyde fixation an...
    Speaker: Guillaume Durin, PhD
    Sponsored By: Covaris, Inc., Covaris, Inc.
    10
    AUG 21, 2013 | 2:00 PM
    C.E. CREDITS
    Comprehensive Chromosome Screening in the Human Embryo
    Comprehensive Chromosome Screening in the Human Embryo
    The human species is particularly prone to chromosome segregation errors during maternal meiosis in the egg and during post zygotic mitosis in the preimplantation embryo. In fact, aneuploidy...
    Speaker: Nathan R Treff, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2013
    33
     
     
  • AUG 21, 2013 | 11:00 AM
    Panel Discussion: Meeting the Demand of Increased NGS Capacity with Automated TruSeq Stranded mRNA Library Preparation
    Panel Discussion: Meeting the Demand of Increased NGS Capacity...
    RNASeq has become the primary method for studying changes in gene expression in modern genomics research. By directly sequencing the various types of RNA molecules, including mRNA, ncRNA, an...
    Speaker: Alisa Jackson, Zach Smith, Mary Blair, Dave Delano
    Presented at: Genetics and Genomics Virtual Event Series 2013
    41
    MAY 30, 2013 | 12:00 PM
    Companion Diagnostics Co-Development: Are you ready?
    Companion Diagnostics Co-Development: Are you ready?
    Development of a companion diagnostic can be critical to the success of a drug. Pharmaceutical companies are committing more and more of their efforts to discover and deliver targeted therapi...
    Speaker: Janice Au-Young, PhD
    Presented at: Clinical Diagnostics Virtual Event Series 2013
    15
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