CRISPR-Cas9: Clustered regularly interspaced short palindromic repeats are segments of prokaryotic DNA containing short, repetitive base sequences. These play a key role in a bacterial defence system,and form the basis of a genome editing technology known as CRISPR/Cas9 that allows permanent modification of genes within organisms. The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as those present within plasmids and phages that provides a form of acquired immunity. It is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA sequence.
This webinar explains how CAR-T cells transfected using Solupore® technology from Avectas and sorted using the CGX10 Cell Isolation System from Sony Biotechnology outperform unsorted cel...
From Discovery to Clinic: Comprehensive CRISPR Solutions for Therapeutic Development CRISPR gene editing has revolutionized the field of molecular biology, offering precise, efficient, and v...
CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR...
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) system is a bacterial defense mechanism that uses RNA-guided enzymes to cut specific s...
Advanced Gene Therapy Development Solutions: A Comprehensive Platform for Next-Generation Therapeutic Innovation: The landscape of gene therapy has evolved dramatically over the past decade,...
Identifying and verifying low frequencies of genomic alterations resulting from off-target editing, gRNA synthesis errors, cross-contamination, or other unintended gRNA activity is critical...
Originally developed for gene editing, CRISPR-Cas systems have now become powerful molecular diagnostic tools. However, their widespread adoption in diagnostics has relied heavily on traditi...
Pancreatic ductal adenocarcinoma (PDAC) is characterized clinically by poor survival and mechanistically by KRAS- and autophagy-dependent growth. We and others previously demonstrated that i...
Coronavirus Disease 2019 (COVID-19) has driven the use of nucleic acid testing technology in clinical testing. There is a growing need for point-of-care testing (POCT)......
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We present programmable ad...
In this talk, I will introduce two new tools that we developed based on CRISPR-enabled gene regulation in bacteria, and one method based on P1 phagemid-enabled delivery of Cas9 nuclease. Fir...
CRISPR-based diagnostics offer rapid and highly sensitive detection of disease biomarkers. Their ease of use and compatibility with lateral-flow readouts make them suitable for point-of-care...
Endonuclease Cas12a, as one key part of the CRISPR/Cas biotechnology, possessed a unique dual enzymatic function which has been widely used for various applications1. However, the limits tra...
Molecular diagnostics based on clustered regularly interspaced short palindromic repeats (CRISPR) enzyme systems have been the subject of intense research....
Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindr...
Inefficient knock-in of transgene cargos limits the potential of cell-based medicines. In this study, we used a CRISPR nuclease that targets a site within an exon of an essential gene and de...
Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
Date: March 08, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Engineered nucleases like the CRISPR-Cas9 system provide researchers with powerful tools for the manipulation of the geno...
Date: December 13, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeu...
First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
Date: October 04, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) The ability to derive induced pluripotent stem cells (iPSCs) from differentiated cell types such as fibroblasts, has r...
Date: September 15, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Tyrosine kinases, a ubiquitous and diverse family of enzymes, are of considerable clinical interest because they dir...
This webinar explains how CAR-T cells transfected using Solupore® technology from Avectas and sorted using the CGX10 Cell Isolation System from Sony Biotechnology outperform unsorted cel...
From Discovery to Clinic: Comprehensive CRISPR Solutions for Therapeutic Development CRISPR gene editing has revolutionized the field of molecular biology, offering precise, efficient, and v...
CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR...
The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated proteins (Cas) system is a bacterial defense mechanism that uses RNA-guided enzymes to cut specific s...
Advanced Gene Therapy Development Solutions: A Comprehensive Platform for Next-Generation Therapeutic Innovation: The landscape of gene therapy has evolved dramatically over the past decade,...
Identifying and verifying low frequencies of genomic alterations resulting from off-target editing, gRNA synthesis errors, cross-contamination, or other unintended gRNA activity is critical...
Originally developed for gene editing, CRISPR-Cas systems have now become powerful molecular diagnostic tools. However, their widespread adoption in diagnostics has relied heavily on traditi...
Pancreatic ductal adenocarcinoma (PDAC) is characterized clinically by poor survival and mechanistically by KRAS- and autophagy-dependent growth. We and others previously demonstrated that i...
Coronavirus Disease 2019 (COVID-19) has driven the use of nucleic acid testing technology in clinical testing. There is a growing need for point-of-care testing (POCT)......
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We present programmable ad...
In this talk, I will introduce two new tools that we developed based on CRISPR-enabled gene regulation in bacteria, and one method based on P1 phagemid-enabled delivery of Cas9 nuclease. Fir...
CRISPR-based diagnostics offer rapid and highly sensitive detection of disease biomarkers. Their ease of use and compatibility with lateral-flow readouts make them suitable for point-of-care...
Endonuclease Cas12a, as one key part of the CRISPR/Cas biotechnology, possessed a unique dual enzymatic function which has been widely used for various applications1. However, the limits tra...
Molecular diagnostics based on clustered regularly interspaced short palindromic repeats (CRISPR) enzyme systems have been the subject of intense research....
Rather than being famous in gene editing field only, by revealing the collateral cleavage activity of Cas12a, Cas13a, Cas14 effectors, and more, Clustered Regularly Interspaced Short Palindr...
Inefficient knock-in of transgene cargos limits the potential of cell-based medicines. In this study, we used a CRISPR nuclease that targets a site within an exon of an essential gene and de...
Date: March 08, 2023 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CET) An unbiased characterization of the human microbiome is crucial to a more complete understanding of microbial effect on h...
Date: March 08, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Engineered nucleases like the CRISPR-Cas9 system provide researchers with powerful tools for the manipulation of the geno...
Date: December 13, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeu...
First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
Date: October 04, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) The ability to derive induced pluripotent stem cells (iPSCs) from differentiated cell types such as fibroblasts, has r...
Date: September 15, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 6:00pm (CEST) Tyrosine kinases, a ubiquitous and diverse family of enzymes, are of considerable clinical interest because they dir...
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