Protein Binding: Protein binding can enhance or detract from a drug's performance. As a general rule, agents that are minimally protein bound penetrate tissue better than those that are highly bound, but they are excreted much faster. Among drugs that are less than 80-85 percent protein bound, differences appear to be of slight clinical importance. Agents that are highly protein bound may, however, differ markedly from those that are minimally bound in terms of tissue penetration and half-life. Drugs may bind to a wide variety of plasma proteins, including albumin. If the percentage of protein-bound drug is greater when measured in human blood than in a simple albumin solution, the clinician should suspect that the agent may be bound in vivo to one of these "minority" plasma proteins. The concentration of several plasma proteins can be altered by many factors, including stress, surgery, liver or kidney dysfunction, and pregnancy. In such circumstances, free drug concentrations are a more accurate index of clinical effect than are total concentrations. Formulary committees must grasp the clinical significance of qualitative and quantitative differences in protein binding when evaluating competing agents.
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Surface plasmon resonance is a real-time and label-free technique that offers low sample consumption and high throughput when compared to other biophysical techniques and has been widely ado...
SPRm (Surface Plasmon Resonance Microscopy) is a novel technology that enables label-free and real-time measurements of the binding affinity and kinetics of small molecules to G protein-coup...
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Phosphorylation of α-synuclein at the Serine-129 site (α-syn Ser129P) is an established pathologic hallmark of synucleinopathies and a therapeutic target. In physiologic states,...
Genetically modified cell models accelerate the development of safe and effective therapeutics, making them indispensable in the quest for new drugs. Through manipulation of cell line geneti...
Affinite Instruments is a developer and supplier of SPR analysis instruments, related consumables, and software. Focusing on the mission of SPR for everyone, this webinar will review the ben...
In general, once a receptor drug target has been validated for the treatment of a particular condition, either a high throughput screen is carried out to identify potential hits, or existing...
In this project, we optimized a high-throughput fluorescent-based assay to assess transporter activity by measuring uptake of the substrate, ASP+ ï€ 4-(4-(dimethylamino)styryl)-N-methylpyridi...
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