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    Protein Purification

    Protein Purification: is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest.

    Webinars

    • SEP 27, 2017 | 12:00 PM
      CRISPR-Cas9 genome engineering: Experimental Design and Applications
      CRISPR-Cas9 genome engineering: Experimental Design and Applications
      The manipulation of the genome to study disease, evaluate drug targets and to stratify patient populations is now a widely accepted concept in the field. At Horizon Discovery, genome engineer...
      Speaker: Danielle Folkard
      Presented at: Cell Biology Virtual Event Series 2017
      Sponsored By: Horizon Discovery
      2
      SEP 27, 2017 | 9:00 AM
      Regulation of actin during cell migration by nucleotide coding sequence and arginylation
      Regulation of actin during cell migration by nucleotide coding...
      Actin cytoskeleton drives cell migration in muscle and non-muscle cells and plays a major role in such diverse processes as tissue morphogenesis, muscle contraction, and cancer metastases. In...
      Speaker: Anna Kashina, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 7:30 AM
      Keynote Presentation: What Microscopy Can Tell Us About the Life of an mRNA in Living Cells
      Keynote Presentation: What Microscopy Can Tell Us About the Life...
      Einstein researcher Robert Singer, Ph.D., discusses a breakthrough in microscopy that is allowing scientists to track messenger RNA in living cells in real time. The study, published in the S...
      Speaker: Robert Singer, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 27, 2017 | 6:00 AM
      Regulation of microtubule plus-end dynamics during axon guidance and cell migration
      Regulation of microtubule plus-end dynamics during axon guidance...
      A fundamental question in neuronal development is how growth cone cytoskeletal dynamics are coordinated to promote accurate axonal navigation. To address this question, we focus on microtubul...
      Speaker: Laura Lowery, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 07, 2017 | 6:00 AM
      Improve your protein research knowledge by joining our 7 Steps of Protein virtual event!
      Improve your protein research knowledge by joining our 7 Steps...
      The 7 Steps of Protein virtual event offers both broad and in-depth content, designed to give you the information and insights you need to empower your protein research every step of the way....
      Speaker:
      Presented at: Protein and Cell Analysis Education Series
      18 1
      JUN 07, 2017 | 9:00 AM
      WEBINAR: Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflow
      WEBINAR: Generation of purified human iPSC derived cardiomyocytes...
      DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
      Speaker: Todd Herron, BS, PhD
      Sponsored By: Miltenyi Biotec
      5 1
      MAY 10, 2017 | 4:00 PM
      Ensuring successful protein expression through proper plasmid purification
      Ensuring successful protein expression through proper plasmid...
      Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
      Speaker: Sasha Vlassov, PhD
      Presented at: 2017 Gibco ExpressionWorld
      3
      MAY 10, 2017 | 2:00 PM
      Selecting the right resin to achieve optimal results, from high throughput batch to process scale protein purification
      Selecting the right resin to achieve optimal results, from high...
      The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
      Speaker: Aaron McBride , Scott Meier
      Presented at: 2017 Gibco ExpressionWorld
      7
      MAY 10, 2017 | 12:00 PM
      Ask the Expert: Mammalian Transient Expression
      Ask the Expert: Mammalian Transient Expression
      Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
      Speaker: Henry Chiou, PhD , Richard Altman, MS , Jon Zmuda, PhD , Konrad Miatkowski
      Presented at: 2017 Gibco ExpressionWorld
      3
      MAY 10, 2017 | 11:00 AM
      An ongoing evaluation of our transient expression systems; Can we predict the appropriate expression system for any drug modality?
      An ongoing evaluation of our transient expression systems; Can...
      CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells.  The ability to e...
      Speaker: Richard Altman, MS
      Presented at: 2017 Gibco ExpressionWorld
      4
      MAY 10, 2017 | 10:00 AM
      ExpiCHO expression of HIV-1 immunogens for structure guided vaccine design
      ExpiCHO expression of HIV-1 immunogens for structure guided vaccine...
      The isolation of broadly-neutralizing antibodies from HIV-1 infected individuals raises the hope that a vaccine may elicit a similar immune response. These antibodies target the only viral pr...
      Speaker: Gabriel Ozorowski, PhD
      Presented at: 2017 Gibco ExpressionWorld
      4
      MAY 10, 2017 | 9:00 AM
      A Semi-Automated High-Throughput Transient Expression and Purification Platform using the Expi293 Expression System
      A Semi-Automated High-Throughput Transient Expression and Purification...
      We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stoc...
      Speaker: Matthew McKenna
      Presented at: 2017 Gibco ExpressionWorld
      4
      MAY 10, 2017 | 5:00 AM
      Complete workflow solution from gene-to-protein combine highly advanced expression systems with expression optimized genes
      Complete workflow solution from gene-to-protein combine highly...
      Reliable autologous expression of recombinant human proteins - from gene sequence to the expressed and purified protein - in human or CHO cells is essential for many aspects of biomedical res...
      Speaker: Stephan Fath, PhD
      Presented at: 2017 Gibco ExpressionWorld
      4 1
      APR 05, 2017 | 8:30 AM
      Gaining quick insights in protein purification - compare results & integrate peaks
      Gaining quick insights in protein purification - compare results...
      UNICORN™ software provides built-in knowledge for planning, controlling, and analyzing protein purification runs on ÄKTA™ chromatography systems. With UNICORN 7, the evalu...
      Speaker: Lena Nyholm, PhD
      Presented at: Protein Research Live
      7 1
      APR 05, 2017 | 8:00 AM
      New generation Size Exclusion Chromatography Columns -  What is in it for you?
      New generation Size Exclusion Chromatography Columns - What is...
      Size exclusion chromatography (SEC) is an extremely powerful technique to separate biomolecules based on differences in size under native conditions. If you are using the SEC technique to ...
      Speaker: Pia Liljedahl
      Presented at: Protein Research Live
      6
      NOV 10, 2016 | 7:30 AM
      Does your protein analysis toolbox need a refresh?
      Does your protein analysis toolbox need a refresh?
      DATE:  November 10, 2016 TIME:  7:30am PT, 10:30am ET  Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each migh...
      Speaker: Rich Jones, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      4
      SEP 08, 2016 | 7:30 AM
      The Molecular Pathology Perspective of the CDC's Infectious Diseases Pathology Branch
      The Molecular Pathology Perspective of the CDC's Infectious Diseases...
      The Infectious Diseases Pathology Branch of the Centers for Disease Control and Prevention (CDC) routinely receives autopsy and biopsy tissues for diagnostic evaluation. These tissues are typ...
      Speaker: Amy Denison, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2016
      4
      JUN 14, 2016 | 8:00 AM
      Rethinking the timeline required for Quality by Design studies in protein process development
      Rethinking the timeline required for Quality by Design studies...
      DATE: June 14, 2016TIME:  8am Pacific time, 11am Eastern timeImproving systems for rapid small-scale protein purification and analysis in order to obtain sample numbers for statist...
      Speaker: Louis Fabri , Bill Flaherty
      Sponsored By: Revvity
      15
      JUN 02, 2016 | 8:00 AM
      WEBINAR: CE-SELEX:  Isolating High Affinity Aptamers Using Capillary Electrophoresis
      WEBINAR: CE-SELEX: Isolating High Affinity Aptamers Using Capillary...
           SELEX is a process that selects DNA or RNA from a random library of sequences based on their affinity for a target molecule.  These high affinity ligands, called apta...
      Speaker: Michael Bowser, PhD
      Sponsored By: Sciex, Sciex
      4 1
      MAY 04, 2016 | 1:00 PM
      Mammalian in vitro translation (cell-free) systems for rapid protein expression and production of therapeutic proteins
      Mammalian in vitro translation (cell-free) systems for rapid protein...
      In vitro translation (IVT) using mammalian cell extracts is a quick and convenient alternative to in vivo mammalian protein expression. Thermo Fisher has developed IVT systems from two mammal...
      Speaker: Penny Jensen, PhD , Govind Rao, PhD
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAY 04, 2016 | 11:00 AM
      Live Chat in the Presentation Hall  - Ask the Expert: Mammalian Transient Expression
      Live Chat in the Presentation Hall - Ask the Expert: Mammalian...
      Join us in the Presentation Hall to chat with our Expert on the topic of Mammalian Transient Expression.  Do you have questions on the differences/similarities between the Expi293 an...
      Speaker: Jon Zmuda, PhD
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAY 04, 2016 | 10:00 AM
      The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification
      The ExpiCHO transient expression system: Optimization of monoclonal...
      The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system,...
      Speaker: Lina Mpanda, MSc.
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAY 04, 2016 | 8:00 AM
      Transient Expression: High Cell Density Culture for Increased Protein Production
      Transient Expression: High Cell Density Culture for Increased...
      The talk will focus on the recent advances in transient protein production using high cell density culture e.g. ExpiCHO, Expi293 for supporting pre-clinical and clinical studies including rob...
      Speaker: Puneet Khandelwal, PhD
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAY 04, 2016 | 5:00 AM
      Complete workflow solution from gene-to-protein
      Complete workflow solution from gene-to-protein
      Reliability from gene sequence to the expressed and purified protein is essential for many aspects of basic research and drug development. Thermo Fisher Scientific’s complete portfolio ...
      Speaker: Michael Spring
      Presented at: 2016 Gibco ExpressionWorld
      2
    SEP 27, 2017 | 12:00 PM
    CRISPR-Cas9 genome engineering: Experimental Design and Applications
    CRISPR-Cas9 genome engineering: Experimental Design and Applications
    The manipulation of the genome to study disease, evaluate drug targets and to stratify patient populations is now a widely accepted concept in the field. At Horizon Discovery, genome engineer...
    Speaker: Danielle Folkard
    Presented at: Cell Biology Virtual Event Series 2017
    Sponsored By: Horizon Discovery
    2
    SEP 27, 2017 | 9:00 AM
    Regulation of actin during cell migration by nucleotide coding sequence and arginylation
    Regulation of actin during cell migration by nucleotide coding...
    Actin cytoskeleton drives cell migration in muscle and non-muscle cells and plays a major role in such diverse processes as tissue morphogenesis, muscle contraction, and cancer metastases. In...
    Speaker: Anna Kashina, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 7:30 AM
    Keynote Presentation: What Microscopy Can Tell Us About the Life of an mRNA in Living Cells
    Keynote Presentation: What Microscopy Can Tell Us About the Life...
    Einstein researcher Robert Singer, Ph.D., discusses a breakthrough in microscopy that is allowing scientists to track messenger RNA in living cells in real time. The study, published in the S...
    Speaker: Robert Singer, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 27, 2017 | 6:00 AM
    Regulation of microtubule plus-end dynamics during axon guidance and cell migration
    Regulation of microtubule plus-end dynamics during axon guidance...
    A fundamental question in neuronal development is how growth cone cytoskeletal dynamics are coordinated to promote accurate axonal navigation. To address this question, we focus on microtubul...
    Speaker: Laura Lowery, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 07, 2017 | 6:00 AM
    Improve your protein research knowledge by joining our 7 Steps of Protein virtual event!
    Improve your protein research knowledge by joining our 7 Steps...
    The 7 Steps of Protein virtual event offers both broad and in-depth content, designed to give you the information and insights you need to empower your protein research every step of the way....
    Speaker:
    Presented at: Protein and Cell Analysis Education Series
    18 1
     
     
  • JUN 07, 2017 | 9:00 AM
    WEBINAR: Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflow
    WEBINAR: Generation of purified human iPSC derived cardiomyocytes...
    DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
    Speaker: Todd Herron, BS, PhD
    Sponsored By: Miltenyi Biotec
    5 1
    MAY 10, 2017 | 4:00 PM
    Ensuring successful protein expression through proper plasmid purification
    Ensuring successful protein expression through proper plasmid...
    Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
    Speaker: Sasha Vlassov, PhD
    Presented at: 2017 Gibco ExpressionWorld
    3
    MAY 10, 2017 | 2:00 PM
    Selecting the right resin to achieve optimal results, from high throughput batch to process scale protein purification
    Selecting the right resin to achieve optimal results, from high...
    The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
    Speaker: Aaron McBride , Scott Meier
    Presented at: 2017 Gibco ExpressionWorld
    7
    MAY 10, 2017 | 12:00 PM
    Ask the Expert: Mammalian Transient Expression
    Ask the Expert: Mammalian Transient Expression
    Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
    Speaker: Henry Chiou, PhD , Richard Altman, MS , Jon Zmuda, PhD , Konrad Miatkowski
    Presented at: 2017 Gibco ExpressionWorld
    3
    MAY 10, 2017 | 11:00 AM
    An ongoing evaluation of our transient expression systems; Can we predict the appropriate expression system for any drug modality?
    An ongoing evaluation of our transient expression systems; Can...
    CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells.  The ability to e...
    Speaker: Richard Altman, MS
    Presented at: 2017 Gibco ExpressionWorld
    4
     
     
  • MAY 10, 2017 | 10:00 AM
    ExpiCHO expression of HIV-1 immunogens for structure guided vaccine design
    ExpiCHO expression of HIV-1 immunogens for structure guided vaccine...
    The isolation of broadly-neutralizing antibodies from HIV-1 infected individuals raises the hope that a vaccine may elicit a similar immune response. These antibodies target the only viral pr...
    Speaker: Gabriel Ozorowski, PhD
    Presented at: 2017 Gibco ExpressionWorld
    4
    MAY 10, 2017 | 9:00 AM
    A Semi-Automated High-Throughput Transient Expression and Purification Platform using the Expi293 Expression System
    A Semi-Automated High-Throughput Transient Expression and Purification...
    We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stoc...
    Speaker: Matthew McKenna
    Presented at: 2017 Gibco ExpressionWorld
    4
    MAY 10, 2017 | 5:00 AM
    Complete workflow solution from gene-to-protein combine highly advanced expression systems with expression optimized genes
    Complete workflow solution from gene-to-protein combine highly...
    Reliable autologous expression of recombinant human proteins - from gene sequence to the expressed and purified protein - in human or CHO cells is essential for many aspects of biomedical res...
    Speaker: Stephan Fath, PhD
    Presented at: 2017 Gibco ExpressionWorld
    4 1
    APR 05, 2017 | 8:30 AM
    Gaining quick insights in protein purification - compare results & integrate peaks
    Gaining quick insights in protein purification - compare results...
    UNICORN™ software provides built-in knowledge for planning, controlling, and analyzing protein purification runs on ÄKTA™ chromatography systems. With UNICORN 7, the evalu...
    Speaker: Lena Nyholm, PhD
    Presented at: Protein Research Live
    7 1
    APR 05, 2017 | 8:00 AM
    New generation Size Exclusion Chromatography Columns -  What is in it for you?
    New generation Size Exclusion Chromatography Columns - What is...
    Size exclusion chromatography (SEC) is an extremely powerful technique to separate biomolecules based on differences in size under native conditions. If you are using the SEC technique to ...
    Speaker: Pia Liljedahl
    Presented at: Protein Research Live
    6
     
     
  • NOV 10, 2016 | 7:30 AM
    Does your protein analysis toolbox need a refresh?
    Does your protein analysis toolbox need a refresh?
    DATE:  November 10, 2016 TIME:  7:30am PT, 10:30am ET  Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each migh...
    Speaker: Rich Jones, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    4
    SEP 08, 2016 | 7:30 AM
    The Molecular Pathology Perspective of the CDC's Infectious Diseases Pathology Branch
    The Molecular Pathology Perspective of the CDC's Infectious Diseases...
    The Infectious Diseases Pathology Branch of the Centers for Disease Control and Prevention (CDC) routinely receives autopsy and biopsy tissues for diagnostic evaluation. These tissues are typ...
    Speaker: Amy Denison, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2016
    4
    JUN 14, 2016 | 8:00 AM
    Rethinking the timeline required for Quality by Design studies in protein process development
    Rethinking the timeline required for Quality by Design studies...
    DATE: June 14, 2016TIME:  8am Pacific time, 11am Eastern timeImproving systems for rapid small-scale protein purification and analysis in order to obtain sample numbers for statist...
    Speaker: Louis Fabri , Bill Flaherty
    Sponsored By: Revvity
    15
    JUN 02, 2016 | 8:00 AM
    WEBINAR: CE-SELEX:  Isolating High Affinity Aptamers Using Capillary Electrophoresis
    WEBINAR: CE-SELEX: Isolating High Affinity Aptamers Using Capillary...
         SELEX is a process that selects DNA or RNA from a random library of sequences based on their affinity for a target molecule.  These high affinity ligands, called apta...
    Speaker: Michael Bowser, PhD
    Sponsored By: Sciex, Sciex
    4 1
    MAY 04, 2016 | 1:00 PM
    Mammalian in vitro translation (cell-free) systems for rapid protein expression and production of therapeutic proteins
    Mammalian in vitro translation (cell-free) systems for rapid protein...
    In vitro translation (IVT) using mammalian cell extracts is a quick and convenient alternative to in vivo mammalian protein expression. Thermo Fisher has developed IVT systems from two mammal...
    Speaker: Penny Jensen, PhD , Govind Rao, PhD
    Presented at: 2016 Gibco ExpressionWorld
    2
     
     
  • MAY 04, 2016 | 11:00 AM
    Live Chat in the Presentation Hall  - Ask the Expert: Mammalian Transient Expression
    Live Chat in the Presentation Hall - Ask the Expert: Mammalian...
    Join us in the Presentation Hall to chat with our Expert on the topic of Mammalian Transient Expression.  Do you have questions on the differences/similarities between the Expi293 an...
    Speaker: Jon Zmuda, PhD
    Presented at: 2016 Gibco ExpressionWorld
    2
    MAY 04, 2016 | 10:00 AM
    The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification
    The ExpiCHO transient expression system: Optimization of monoclonal...
    The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system,...
    Speaker: Lina Mpanda, MSc.
    Presented at: 2016 Gibco ExpressionWorld
    2
    MAY 04, 2016 | 8:00 AM
    Transient Expression: High Cell Density Culture for Increased Protein Production
    Transient Expression: High Cell Density Culture for Increased...
    The talk will focus on the recent advances in transient protein production using high cell density culture e.g. ExpiCHO, Expi293 for supporting pre-clinical and clinical studies including rob...
    Speaker: Puneet Khandelwal, PhD
    Presented at: 2016 Gibco ExpressionWorld
    2
    MAY 04, 2016 | 5:00 AM
    Complete workflow solution from gene-to-protein
    Complete workflow solution from gene-to-protein
    Reliability from gene sequence to the expressed and purified protein is essential for many aspects of basic research and drug development. Thermo Fisher Scientific’s complete portfolio ...
    Speaker: Michael Spring
    Presented at: 2016 Gibco ExpressionWorld
    2
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