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    Protein Isolation

    Protein Isolation: The initial step in protein isolation from its source is to physically or chemically disrupt the biological tissue or organism in order to release the protein into the extract. In some cases, the organism secretes the protein, and cell disruption is unnecessary. The protein is usually produced in dilute concentration so it is advantageous if the initial isolation also results in the concentration of the protein. An important feature in any isolation procedure is product yield with the goal being to extract as much of the desired protein as possible in a minimum amount of time.

    Webinars

    • NOV 15, 2019 | 8:00 AM
      Exosomes and super-resolution microscopy
      Exosomes and super-resolution microscopy
      DATE: November 15, 2019TIME: 8:00am PST, 11:00am EST Exosomes are small vesicles, ranging from 30-120 nanometers in diameter, secreted from cells throughout the human body. The...
      Speaker: Ryan McNamara, PhD
      Sponsored By: ONI
      8
      SEP 12, 2019 | 10:30 AM
      Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
      Analysis of human gut microbiomes and antibiotic resistomes of...
      The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
      Speaker: Jonathan Jacobs, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2019
      Sponsored By: QIAGEN
      5
      SEP 11, 2019 | 1:30 PM
      Development of Machine Learning Algorithms to Data Mine Meta-Omic Characterizations of Complex Microbiota
      Development of Machine Learning Algorithms to Data Mine Meta-Omic...
      Background: The vast majority of all genes are contained within the genomes of the prokaryotes, including the eubacteria and the archaea. These largely single-cellular domains of life thus...
      Speaker: Garth Ehrlich, PhD, FAAAS
      Presented at: Microbiology & Immunology Virtual Event Series 2019
      Sponsored By: PacBio
      4
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
      AUG 22, 2019 | 10:30 AM
      Generating Authentic Research Experiences of Forensic-Based Projects in an Undergraduate Classroom Laboratory
      Generating Authentic Research Experiences of Forensic-Based Projects...
      DNA profiling tools to teach undergraduate students about forensics generally utilize the PV92 Alu and D1S80 VNTR markers, but are both limited in scope. In contrast, advanced profiling syste...
      Speaker: David Kass, PhD
      Presented at: Forensics & Toxicology 2019
      2
      AUG 22, 2019 | 9:00 AM
      Keynote Presentation: New Concepts in Forensic Investigation
      Keynote Presentation: New Concepts in Forensic Investigation
      Contemporary law enforcement has greatly expanded its ability to solve crimes by the adoption of advanced forensic techniques, electronic monitoring and new approaches in crime scene procedur...
      Speaker: Henry C. Lee, PhD
      Presented at: Forensics & Toxicology 2019
      8 1
      AUG 22, 2019 | 7:30 AM
      Keynote Presentation: Health Impacts of Environmental Chemical Exposures
      Keynote Presentation: Health Impacts of Environmental Chemical...
      The incidence of conditions such as diabetes, obesity, asthma, and cancer have increased substantially in the past 30 years. The human genome has not changed in that period of time, so the en...
      Speaker: Linda Birnbaum, PhD
      Presented at: Forensics & Toxicology 2019
      5
      AUG 22, 2019 | 6:00 AM
      In Vitro Screening Approaches to Estimate Human Hepatic Responses to Xenobiotic Exposures
      In Vitro Screening Approaches to Estimate Human Hepatic Responses...
      Prediction of human response to chemical exposures is the primary challenge of pharmaceutical and environmental toxicology research. The complexity of human responses to xenobiotic exposures...
      Speaker: Stephen Ferguson, PhD
      Presented at: Forensics & Toxicology 2019
      1
      JUN 05, 2019 | 5:00 PM
      Everyday culture practice - Improving reproducibility in cell culture
      Everyday culture practice - Improving reproducibility in cell...
      DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
      Speaker: Jessica Wagener, PhD
      Sponsored By: Eppendorf
      23 2
      MAY 16, 2019 | 4:00 PM
      Towards Standardized Workflows - A focus on microbiome insights
      Towards Standardized Workflows - A focus on microbiome insights
      DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
      Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
      Sponsored By: Eppendorf
      10 2
      FEB 27, 2019 | 3:00 PM
      Automation Meets Cell Separation: Primary Cells for Drug Discovery Assays
      Automation Meets Cell Separation: Primary Cells for Drug Discovery...
      Whether you are performing killing assays, immunophenotyping of TILs, characterizing the tumor microenvironment, or investigating immunogenicity, primary cells are essential for the discovery...
      Speaker: Lotta Raty
      Presented at: Drug Discovery Virtual Event Series 2019
      Sponsored By: Miltenyi Biotec
      2
      OCT 09, 2018 | 8:00 AM
      GMP Ancillary Materials for Cell and Gene Therapy Manufacturing: What to consider and when to make the switch
      GMP Ancillary Materials for Cell and Gene Therapy Manufacturing:...
      DATE: October 9, 2018TIME: 08:00am PDT, 11:00pm EDT Have you ever wondered what to consider when you are selecting ancillary materials for your cell therapy clinical research...
      Speaker: Kasey Kime , Eric Roos
      Sponsored By: Thermo Fisher Scientific/Gibco
      12
      SEP 27, 2018 | 7:30 AM
      Keynote Presentation: Mapping the Inner World of the Cell
      Keynote Presentation: Mapping the Inner World of the Cell
      Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...
      Speaker: Bo Huang, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      1
      SEP 26, 2018 | 12:00 PM
      3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
      3D Cell Cultures and Tissue Clearing: A High Content Confocal...
      With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
      Speaker: Michael Johnson, PhD
      Presented at: Cell Biology Virtual Event Series 2018
      2
      SEP 18, 2018 | 8:00 AM
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
      Speaker: Judd Hultquist, PhD
      Sponsored By: Horizon Discovery
      7
      MAY 17, 2018 | 12:00 PM
      Cryo Electron Microscopy: Revolutionizing the world of structural biology and healthcare
      Cryo Electron Microscopy: Revolutionizing the world of structural...
      Studying the molecular machinery of cells from atomic detail to the cellular context and beyond is a great challenge for cell biology. It is hugely important to zoom into the atom-by-atom arr...
      Speaker: Marc Storms
      Presented at: Gibco ExpressionWorld 2018
      7
      MAY 09, 2018 | 9:00 AM
      Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
      Gene Expression Analysis - Critical Factors for Overcoming Challenges...
      Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
      Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      MAY 03, 2018 | 11:00 AM
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
      DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
      Speaker: Sisi Li
      Sponsored By: Covaris
      5 2
      MAR 27, 2018 | 9:00 AM
      How to use CRISPR-Cas9 for knockout, knock-in and gene activation
      How to use CRISPR-Cas9 for knockout, knock-in and gene activation
      DATE: March 27, 2018TIME: 09:00am PDT, 12:00pm EDTThe rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, a...
      Speaker: Amanda Haas
      Sponsored By: Horizon Discovery
      23 15
      FEB 01, 2018 | 8:00 AM
      High capacity magnetic supports for automated antibody and epitope-tagged protein purifications
      High capacity magnetic supports for automated antibody and epitope-tagged...
      For academic, biotech, and pharmaceutical scientists who are screening clones or performing high throughput protein purification, the goal is to automate the sample processing without sacrifi...
      Speaker: Barbara Kaboord, PhD
      Presented at: Gibco ExpressionWorld 2018
      2
      SEP 28, 2017 | 1:30 PM
      The RRID Initiative, what is needed for scholarship in the 21st century
      The RRID Initiative, what is needed for scholarship in the 21st...
      The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
      Speaker: Anita Bandrowski, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 12:00 PM
      Back to basics: Fundamental concepts and special considerations in RNA isolation
      Back to basics: Fundamental concepts and special considerations...
      How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
      Speaker: Abhishek Sharma
      Presented at: Cell Biology Virtual Event Series 2017
      2
      SEP 28, 2017 | 10:30 AM
      Engineering stem cell biology for disease modelling and therapeutics
      Engineering stem cell biology for disease modelling and therapeutics
      Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
      Speaker: Amr Abdeen, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
      SEP 28, 2017 | 9:00 AM
      Fine tuning of myosin activity shapes actomyosin network organization and tissue contractility of the C. elegans spermatheca
      Fine tuning of myosin activity shapes actomyosin network organization...
      Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
      Speaker: Alison Wirshing, PhD
      Presented at: Cell Biology Virtual Event Series 2017
      1
    NOV 15, 2019 | 8:00 AM
    Exosomes and super-resolution microscopy
    Exosomes and super-resolution microscopy
    DATE: November 15, 2019TIME: 8:00am PST, 11:00am EST Exosomes are small vesicles, ranging from 30-120 nanometers in diameter, secreted from cells throughout the human body. The...
    Speaker: Ryan McNamara, PhD
    Sponsored By: ONI
    8
    SEP 12, 2019 | 10:30 AM
    Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
    Analysis of human gut microbiomes and antibiotic resistomes of...
    The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
    Speaker: Jonathan Jacobs, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2019
    Sponsored By: QIAGEN
    5
    SEP 11, 2019 | 1:30 PM
    Development of Machine Learning Algorithms to Data Mine Meta-Omic Characterizations of Complex Microbiota
    Development of Machine Learning Algorithms to Data Mine Meta-Omic...
    Background: The vast majority of all genes are contained within the genomes of the prokaryotes, including the eubacteria and the archaea. These largely single-cellular domains of life thus...
    Speaker: Garth Ehrlich, PhD, FAAAS
    Presented at: Microbiology & Immunology Virtual Event Series 2019
    Sponsored By: PacBio
    4
    SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
    17 1
    AUG 22, 2019 | 10:30 AM
    Generating Authentic Research Experiences of Forensic-Based Projects in an Undergraduate Classroom Laboratory
    Generating Authentic Research Experiences of Forensic-Based Projects...
    DNA profiling tools to teach undergraduate students about forensics generally utilize the PV92 Alu and D1S80 VNTR markers, but are both limited in scope. In contrast, advanced profiling syste...
    Speaker: David Kass, PhD
    Presented at: Forensics & Toxicology 2019
    2
     
     
  • AUG 22, 2019 | 9:00 AM
    Keynote Presentation: New Concepts in Forensic Investigation
    Keynote Presentation: New Concepts in Forensic Investigation
    Contemporary law enforcement has greatly expanded its ability to solve crimes by the adoption of advanced forensic techniques, electronic monitoring and new approaches in crime scene procedur...
    Speaker: Henry C. Lee, PhD
    Presented at: Forensics & Toxicology 2019
    8 1
    AUG 22, 2019 | 7:30 AM
    Keynote Presentation: Health Impacts of Environmental Chemical Exposures
    Keynote Presentation: Health Impacts of Environmental Chemical...
    The incidence of conditions such as diabetes, obesity, asthma, and cancer have increased substantially in the past 30 years. The human genome has not changed in that period of time, so the en...
    Speaker: Linda Birnbaum, PhD
    Presented at: Forensics & Toxicology 2019
    5
    AUG 22, 2019 | 6:00 AM
    In Vitro Screening Approaches to Estimate Human Hepatic Responses to Xenobiotic Exposures
    In Vitro Screening Approaches to Estimate Human Hepatic Responses...
    Prediction of human response to chemical exposures is the primary challenge of pharmaceutical and environmental toxicology research. The complexity of human responses to xenobiotic exposures...
    Speaker: Stephen Ferguson, PhD
    Presented at: Forensics & Toxicology 2019
    1
    JUN 05, 2019 | 5:00 PM
    Everyday culture practice - Improving reproducibility in cell culture
    Everyday culture practice - Improving reproducibility in cell...
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    Speaker: Jessica Wagener, PhD
    Sponsored By: Eppendorf
    23 2
    MAY 16, 2019 | 4:00 PM
    Towards Standardized Workflows - A focus on microbiome insights
    Towards Standardized Workflows - A focus on microbiome insights
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    Speaker: Rita Colwell, PhD , Christiane Honisch, PhD
    Sponsored By: Eppendorf
    10 2
     
     
  • FEB 27, 2019 | 3:00 PM
    Automation Meets Cell Separation: Primary Cells for Drug Discovery Assays
    Automation Meets Cell Separation: Primary Cells for Drug Discovery...
    Whether you are performing killing assays, immunophenotyping of TILs, characterizing the tumor microenvironment, or investigating immunogenicity, primary cells are essential for the discovery...
    Speaker: Lotta Raty
    Presented at: Drug Discovery Virtual Event Series 2019
    Sponsored By: Miltenyi Biotec
    2
    OCT 09, 2018 | 8:00 AM
    GMP Ancillary Materials for Cell and Gene Therapy Manufacturing: What to consider and when to make the switch
    GMP Ancillary Materials for Cell and Gene Therapy Manufacturing:...
    DATE: October 9, 2018TIME: 08:00am PDT, 11:00pm EDT Have you ever wondered what to consider when you are selecting ancillary materials for your cell therapy clinical research...
    Speaker: Kasey Kime , Eric Roos
    Sponsored By: Thermo Fisher Scientific/Gibco
    12
    SEP 27, 2018 | 7:30 AM
    Keynote Presentation: Mapping the Inner World of the Cell
    Keynote Presentation: Mapping the Inner World of the Cell
    Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principle...
    Speaker: Bo Huang, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    1
    SEP 26, 2018 | 12:00 PM
    3D Cell Cultures and Tissue Clearing: A High Content Confocal Platform for the Complete 3D Characterization of Advanced Cell Models
    3D Cell Cultures and Tissue Clearing: A High Content Confocal...
    With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-...
    Speaker: Michael Johnson, PhD
    Presented at: Cell Biology Virtual Event Series 2018
    2
    SEP 18, 2018 | 8:00 AM
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
    Speaker: Judd Hultquist, PhD
    Sponsored By: Horizon Discovery
    7
     
     
  • MAY 17, 2018 | 12:00 PM
    Cryo Electron Microscopy: Revolutionizing the world of structural biology and healthcare
    Cryo Electron Microscopy: Revolutionizing the world of structural...
    Studying the molecular machinery of cells from atomic detail to the cellular context and beyond is a great challenge for cell biology. It is hugely important to zoom into the atom-by-atom arr...
    Speaker: Marc Storms
    Presented at: Gibco ExpressionWorld 2018
    7
    MAY 09, 2018 | 9:00 AM
    Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
    Gene Expression Analysis - Critical Factors for Overcoming Challenges...
    Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
    Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
    MAY 03, 2018 | 11:00 AM
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    Speaker: Sisi Li
    Sponsored By: Covaris
    5 2
    MAR 27, 2018 | 9:00 AM
    How to use CRISPR-Cas9 for knockout, knock-in and gene activation
    How to use CRISPR-Cas9 for knockout, knock-in and gene activation
    DATE: March 27, 2018TIME: 09:00am PDT, 12:00pm EDTThe rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, a...
    Speaker: Amanda Haas
    Sponsored By: Horizon Discovery
    23 15
    FEB 01, 2018 | 8:00 AM
    High capacity magnetic supports for automated antibody and epitope-tagged protein purifications
    High capacity magnetic supports for automated antibody and epitope-tagged...
    For academic, biotech, and pharmaceutical scientists who are screening clones or performing high throughput protein purification, the goal is to automate the sample processing without sacrifi...
    Speaker: Barbara Kaboord, PhD
    Presented at: Gibco ExpressionWorld 2018
    2
     
     
  • SEP 28, 2017 | 1:30 PM
    The RRID Initiative, what is needed for scholarship in the 21st century
    The RRID Initiative, what is needed for scholarship in the 21st...
    The Resource Identification Initiative supports NIH's new guidelines for Rigor and Transparency as these apply in biomedical publications. Authors are instructed to authenticate key biolo...
    Speaker: Anita Bandrowski, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 12:00 PM
    Back to basics: Fundamental concepts and special considerations in RNA isolation
    Back to basics: Fundamental concepts and special considerations...
    How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
    Speaker: Abhishek Sharma
    Presented at: Cell Biology Virtual Event Series 2017
    2
    SEP 28, 2017 | 10:30 AM
    Engineering stem cell biology for disease modelling and therapeutics
    Engineering stem cell biology for disease modelling and therapeutics
    Stem cells can self-renew and differentiate into multiple different cell types. Engineering of stem cells has enabled new methods to study development and organogenesis in humans as well as d...
    Speaker: Amr Abdeen, PhD
    Presented at: Cell Biology Virtual Event Series 2017
    1
    SEP 28, 2017 | 9:00 AM
    Fine tuning of myosin activity shapes actomyosin network organization and tissue contractility of the C. elegans spermatheca
    Fine tuning of myosin activity shapes actomyosin network organization...
    Contractile non-muscle cells, including smooth muscle and myoepithelial cells, provide the mechanical forces required for tissue homeostasis in numerous organ systems. For example, smooth mus...
    Speaker: Alison Wirshing, PhD
    Presented at: Cell Biology Virtual Event Series 2017
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