The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. In brief, the sample undergoes protein denaturation, followed by gel electrophoresis. Synthetic or animal-derived antibodies are created that recognise and bind to a specific target protein, known as the primary antibody. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody. The secondary antibody is visualised through various methods such as staining, immunofluorescence, and radioactivity, allowing indirect detection of the specific target protein.
Two projects looking at novel approaches to targeting inflammatory breast cancer will be presented. Inflammatory breast cancer (IBC) is a unique, understudied, and most lethal subtype account...
The oncogenic transcription factor c-MYC (MYC) is deregulated, and often overexpressed, in more than 50% of cancers. MYC deregulation is associated with poor prognosis and aggressive disease,...
In the past two decades a small number of infrequently dividing cells have been proposed as the source of multi-drug resistance during cancer treatment. These cells identified by their expres...
PacBio Sequencing simultaneously provides long sequence reads, high consensus accuracy, minimal sequence bias, and methylation detection. I will highlight new advances and updates on applying...
RNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving hig...
To date the anatomic extent of tumor (TNM-classification) has been by far the most important factors to predict the prognosis of cancer patients. However, this classification provides limited...
In prior work, we have pursued how tumor reactive monoclonal antibody (mAb), together with activators of innate immune cells, like NK cells, can augment antibody dependent cell-mediated cytot...
High-throughput screening is widely useful in identifying genes and pathways that drive changes in cell behavior such as cell cycle regulation, metastasis, and drug resistance. Millipor...
Molecular analyses of cancer biology have tended to segregate between a focus on nucleic acids – DNA, RNA and their modifications – and a focus on proteins and protein function. P...
Understanding the immune repertoire is an important aspect of immuno-oncology research, which can be used to gather insights into the function and overall status of the immune system. We have...
DATE: June 5, 2018TIME: 06:30AM PDT, 3:30PM CEST Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either...
DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
In order to expand the treatment options of cancers driven by oncogenic RAS, new cell surface targets need to be identified and characterized. Here, we describe mass spectrometry based phenot...
The 7 Steps of Protein virtual event offers both broad and in-depth content, designed to give you the information and insights you need to empower your protein research every step of the way....
Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders Reproducibility and antibody validation standards are two significant challenges f...
DATE: May 18, 2017TIME: 8:00AM PDT, 11:00AM ETIt is well established that ligand binding can protect proteins from thermal denaturation in a cellular milieu; however, the broad applicat...
DATE: May 8th, 2017TIME: 10:00AM PT, 11:00AM MT, 12:00PM CT, 1:00PM ETThis webinar will describe the potential diagnostic benefits of using the dissociated VIDAS Lyme IgM II and VIDAS L...
DATE: September 22, 2016
TIME: 4:00am PT, 7:00am ET, 11:00am GMT
Here, we present a novel approach that combines the principles of the western blot (protein separation by SDS-PAGE; ...
Two projects looking at novel approaches to targeting inflammatory breast cancer will be presented. Inflammatory breast cancer (IBC) is a unique, understudied, and most lethal subtype account...
The oncogenic transcription factor c-MYC (MYC) is deregulated, and often overexpressed, in more than 50% of cancers. MYC deregulation is associated with poor prognosis and aggressive disease,...
In the past two decades a small number of infrequently dividing cells have been proposed as the source of multi-drug resistance during cancer treatment. These cells identified by their expres...
PacBio Sequencing simultaneously provides long sequence reads, high consensus accuracy, minimal sequence bias, and methylation detection. I will highlight new advances and updates on applying...
RNA sequencing unlocks the mysteries hidden in the transcriptome. Whether your goal is gene expression analysis, gene fusion analysis, SNP analysis or miRNA expression analysis, achieving hig...
To date the anatomic extent of tumor (TNM-classification) has been by far the most important factors to predict the prognosis of cancer patients. However, this classification provides limited...
In prior work, we have pursued how tumor reactive monoclonal antibody (mAb), together with activators of innate immune cells, like NK cells, can augment antibody dependent cell-mediated cytot...
High-throughput screening is widely useful in identifying genes and pathways that drive changes in cell behavior such as cell cycle regulation, metastasis, and drug resistance. Millipor...
Molecular analyses of cancer biology have tended to segregate between a focus on nucleic acids – DNA, RNA and their modifications – and a focus on proteins and protein function. P...
Understanding the immune repertoire is an important aspect of immuno-oncology research, which can be used to gather insights into the function and overall status of the immune system. We have...
DATE: June 5, 2018TIME: 06:30AM PDT, 3:30PM CEST Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either...
DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
In order to expand the treatment options of cancers driven by oncogenic RAS, new cell surface targets need to be identified and characterized. Here, we describe mass spectrometry based phenot...
The 7 Steps of Protein virtual event offers both broad and in-depth content, designed to give you the information and insights you need to empower your protein research every step of the way....
Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders Reproducibility and antibody validation standards are two significant challenges f...
DATE: May 18, 2017TIME: 8:00AM PDT, 11:00AM ETIt is well established that ligand binding can protect proteins from thermal denaturation in a cellular milieu; however, the broad applicat...
DATE: May 8th, 2017TIME: 10:00AM PT, 11:00AM MT, 12:00PM CT, 1:00PM ETThis webinar will describe the potential diagnostic benefits of using the dissociated VIDAS Lyme IgM II and VIDAS L...
DATE: September 22, 2016
TIME: 4:00am PT, 7:00am ET, 11:00am GMT
Here, we present a novel approach that combines the principles of the western blot (protein separation by SDS-PAGE; ...
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