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The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme...
It was 10 years ago that Illumina first launched the Genome Analyzer II, the world’s first high-throughput sequencing platform. That system could produce one billion bps of sequen...
Nucleotide variation in gene regulatory elements is a major cause of human disease. Despite continual progress in the cataloging of these elements, little is known about the code and grammati...
Genetic disease is the leading cause of death for infants in the intensive care units. Traditional testing is too slow to provide physicians a timely diagnosis to help guide the management of...
The study of inherited genomic variation through genome wide association studies (GWAS) promised to provide key biologic insight into common diseases of public health significance such as obe...
Genetic testing currently plays a relatively niche role in healthcare, with testing typically limited to single genes and targeting a relatively narrow range of diseases, including paediatric...
Boston Children’s Hospital is developing the infrastructure needed for large-scale psychiatric research and treatment discovery. The Manton Center for Orphan Disease Research and...
In this seminar, I will discuss issues in transcriptome analysis. I will first talk about core aspects - how we analyze the activity patterns of genes in model organisms and humans. I will fo...
Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
SMRT Sequencing is a DNA sequencing technology characterized by long read lengths and high consensus accuracy, regardless of the sequence complexity or GC content of the DNA sample. These cha...
Characterizing cellular hierarchy has profound implications in development and diseases. Single-cell technologies have great potential in discovering new cell types and lineage relationships,...
Glycosylation is an important product quality attribute for biotherapeutic proteins expressed in CHO cells. Glycoform variability can significantly affect the safety and efficacy of therapeut...
Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
A plethora of methods and tools have been developed to identify genes differentially expressed between two compared conditions, such as "diseased versus healthy". For many scientist...
Many computational approaches exist for predicting the effects of amino acid substitutions from protein sequence. These are often (incorrectly) used for judging disease predisposition from in...
CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells. The ability to e...
Although ultrahigh-throughput RNA-sequencing has become the dominant technology for genome-wide transcriptional profiling, the vast majority of RNA-seq studies typically profile only tens of...
The realization of the promise of personalized molecular medicine requires efficient development and implementation of novel targeted therapeutics linked to molecular markers able to identify...
The isolation of broadly-neutralizing antibodies from HIV-1 infected individuals raises the hope that a vaccine may elicit a similar immune response. These antibodies target the only viral pr...
We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stoc...
Genome Wide Association Studies (GWAS) and expression quantitative trait locus (eQTL) analyses have identified genetic associations with a wide range of human phenotypes. However, many of the...
Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
Do you have targets that are difficult to genotype? Are you starting new projects and looking for a fast convenient genotyping solution?Whether screening CRISPR clones, exploring pharmaco...
Cell free DNA (cfDNA) analysis for the purpose of diagnostics is currently practiced for non-invasive prenatal screening of cell free fetal DNA (cffDNA), and is anticipated to be leveraged fo...
HEK293 and CHO cells are the primary cell lines used in rapid, scalable suspension culture systems for transient protein expression. This presentation provides a primer on fundamental concept...
The nCounter® Analysis System from NanoString® Technologies uses a novel molecular barcoding technology to measure multiplexed gene expression of up to 800 targets with simple, enzyme...
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