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    Cell Signaling

    Cell Signaling: the process of cells communicating with other cells within the body, or with the external environment. As a process, cell signaling refers to a vast network of communication between, and within, each cell of our body.

    Webinars

    • AUG 04, 2021 | 8:00 AM
      The Power of Autofluorescence Extraction Using Full Spectrum Profilingâ„¢
      The Power of Autofluorescence Extraction Using Full Spectrum Profilingâ„¢
      Date: August 4, 2021 Time: 8:00am (PDT), 11:00am (EDT) The intrinsic emission of light by biological structures in the cells, known as autofluorescence (AF), can be detrimental for data reso...
      Speaker: Diana Escobar
      Sponsored By: Cytek Biosciences
      8 1
      JUN 30, 2021 | 7:00 AM
      Optical Genome Mapping: Unrivaled Structural Variant Detection for Cancer Research and Precision Medicine
      Optical Genome Mapping: Unrivaled Structural Variant Detection...
      Date: June 30, 2021 Time: 7:00am (PDT), 8:00am (EDT) Join us to discover how Optical genome mapping (OGM) mapping can help you in the characterization of complex samples. OGM is a non-sequen...
      Speaker: Yannick Delpu, PhD , Darren "Ben" Finlay, PhD
      Sponsored By: Bionano
      8
      JUN 09, 2021 | 7:00 AM
      Preventing cell misidentification in your daily lab routine
      Preventing cell misidentification in your daily lab routine
      Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
      Speaker: Gabriela Saldanha , Jessica Wagener, PhD
      Sponsored By: Eppendorf
      40 3
      MAY 11, 2021 | 10:00 AM
      Secure Your Next Great Discovery
      Secure Your Next Great Discovery
      Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
      Speaker: Paulina Kocjan , Kate Meola , Zareh Zurabyan
      Presented at: Eppendorf Planet of Knowledge - A New Dimension
      Sponsored By: Eppendorf
      8
      APR 21, 2021 | 5:00 PM
      Immunology Researchers share their Spatial Discoveries in SARS-CoV-2 host response and in multiple sclerosis
      Immunology Researchers share their Spatial Discoveries in SARS-CoV-2...
      Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
      Speaker: Doug Brown, PhD , Susanne Krasemann, MD, PhD , Michelle Naughton, PhD
      Sponsored By: NanoString Technologies, Illumina
      5
      APR 20, 2021 | 4:30 PM
      C.E. CREDITS
      High-throughput clinical and environmental viral extraction and sequencing
      High-throughput clinical and environmental viral extraction and...
      COVID-19 is a severe disease that has caused >1 million deaths in under one year. As this disease is novel, the molecular and cellular underpinnings of the progressive tissue injury are p...
      Speaker: Christopher Mason, Ph.D
      Presented at: Genetics Week Virtual Event Series 2021
      Sponsored By: Tecan
      2
      MAR 31, 2021 | 8:00 AM
      CytoFLEX SRT Cell Sorter: What is it really like for a user?
      CytoFLEX SRT Cell Sorter: What is it really like for a user?
      Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
      Speaker: Peter O'Toole, PhD , Dr. Karen Hogg
      Sponsored By: Beckman Coulter Life Sciences
      7
      MAR 17, 2021 | 8:00 AM
      Advanced enzymes to facilitate SARS-CoV-2 research
      Advanced enzymes to facilitate SARS-CoV-2 research
      Date: March 17, 2021 Time: 08:00am (PST) Research focused on SARS-CoV-2 has become a primary focus in genomic and molecular biology fields. With a constantly changing field with new methods...
      Speaker: Li Lavelle
      Sponsored By: Thermo Fisher Scientific
      24 1
      FEB 24, 2021 | 1:30 PM
      Roundtable Panel Presentation: Recent Trends in Screening GPCRs
      Roundtable Panel Presentation: Recent Trends in Screening GPCRs
      G protein-coupled receptors (GPCRs) are among the most intensively studied drug targets, and account for about ~34% of all drugs approved by the FDA. Examples of drugs targeting GPCRs includ...
      Speaker: Annette Gilchrist, PhD , Professor Kevin Pfleger, MA, PhD, FBPhS , Juan José Fung, PhD , Aarti Kawatkar , Yamina A. Berchiche, PhD , Ajay Yekkirala, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      1
      FEB 24, 2021 | 10:00 AM
      Biosafety Enclosures and the Automated Lab: Steps for Success
      Biosafety Enclosures and the Automated Lab: Steps for Success
      DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
      Speaker: David Wasescha , Simon Fogarty
      Sponsored By: Labconco
      15
      FEB 24, 2021 | 12:00 AM
      Preclinical evaluation of novel OT-analogs for the potential treatment of social behavioral deficits
      Preclinical evaluation of novel OT-analogs for the potential treatment...
      As pharmaceutical companies have reduced or eliminated neuroscience drug development due to high costs and low approval rates, the economic and healthcare burden of CNS disorders in the Unit...
      Speaker: Marsha Pierce, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      2
      FEB 24, 2021 | 12:00 AM
      The development and application of TRUPATH biosensors to the study of GPCR pharmacology and functional selectivity
      The development and application of TRUPATH biosensors to the study...
      G-protein-coupled receptors (GPCRs) remain major drug targets, despite an incomplete understanding of how they signal through 16 non-visual G-protein signal transducers (collectively named t...
      Speaker: Reid Olsen, PhD, Pharmacology, Head of GPCR Pharmacology
      Presented at: Drug Discovery & Development Virtual Event Series 2021
      4
      DEC 16, 2020 | 8:00 AM
      Adding Dimensions to Multiplex Molecular Imaging
      Adding Dimensions to Multiplex Molecular Imaging
      Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
      Speaker: Scott Fraser, PhD
      Sponsored By: Leica Microsystems
      8
      DEC 09, 2020 | 9:00 AM
      Human hematopoietic stem cell research made smart and easy
      Human hematopoietic stem cell research made smart and easy
      DATE: December 09, 2020 TIME: 09:00am PST Hematopoietic stem cells (HSCs) ensure homeostasis of blood throughout life. In this webinar, we share insights into the smart and easy isolation, c...
      Speaker: Antje Kremer
      Sponsored By: Miltenyi Biotec
      15 1
      DEC 04, 2020 | 9:00 AM
      IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail - Visualizing immune response during infections
      IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody...
      Date: December 4, 2020 Time: 9:00am PST, 12:00pm EST Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives...
      Speaker: Shankar Pattabhiraman
      Sponsored By: Beckman Coulter Life Sciences
      5
      DEC 03, 2020 | 10:30 AM
      The Immunology of COVID-19: A dynamic immune signature for prognosis and spatial transcriptomics of lung tissue
      The Immunology of COVID-19: A dynamic immune signature for prognosis...
      The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and has spread globally, causing a pandemic of respiratory illness designated coronavirus disease 20...
      Speaker: Arutha Kulasinghe, PhD , Miguel Muñoz-Ruiz, PhD
      Presented at: Coronavirus Series
      Sponsored By: NanoString Technologies
      3
      DEC 02, 2020 | 8:00 AM
      Optimize your bioprocess - From shaker to bioreactor
      Optimize your bioprocess - From shaker to bioreactor
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      Speaker: Ines Hartmann , David Solbach
      Sponsored By: Eppendorf
      11
      NOV 19, 2020 | 9:00 AM
      From Protein Structures to Drug Discovery: Novel therapeutic opportunities.
      From Protein Structures to Drug Discovery: Novel therapeutic opportunities.
      DATE: November 19, 2020 TIME: 9:00am PT, 12am ET Knowledge of the three-dimensional structure of therapeutically relevant targets has become an essential step in the pipeline of drug discove...
      Speaker: Amal Hassan Ali
      Sponsored By: Thermo Fisher Scientific
      16 1
      NOV 19, 2020 | 9:00 AM
      Highly specific multiplexed RNA imaging in tissues with split-FISH
      Highly specific multiplexed RNA imaging in tissues with split-FISH
      Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
      Speaker: Kok Hao Chen , Alan Mullan, PhD
      Sponsored By: Andor
      6 2
      NOV 18, 2020 | 8:00 AM
      Whole-organ mapping using AI and light sheet microscopy
      Whole-organ mapping using AI and light sheet microscopy
      DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
      Speaker: Ali Ertürk
      Sponsored By: Miltenyi Biotec
      14
      NOV 18, 2020 | 7:00 AM
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
      Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
      DATE: November 18, 2020 TIME: 7:00am PST, 10:00am PST, 4:00pm CEST The assessment of cell health and cellular responses after experimental manipulation continue to be a very important......
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf, Promega
      17
      NOV 16, 2020 | 8:00 AM
      Targeted single-cell RNA sequencing enables genome-scale CRISPR screens
      Targeted single-cell RNA sequencing enables genome-scale CRISPR...
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      Speaker: Wieland Keilholz, Ph.D. , Pawel Zajac, Ph.D. , Dr. Tilmann Buerckstuemmer
      Sponsored By: Illumina
      11
      NOV 05, 2020 | 7:00 AM
      Stem cell derived exosome production in stirred-tank bioreactors
      Stem cell derived exosome production in stirred-tank bioreactors
      DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
      Speaker: Jorge Escobar , Aurélie Tacheny
      Presented at: Eppendorf Planet of Knowledge - A New Dimension
      Sponsored By: Eppendorf
      12
      OCT 28, 2020 | 7:00 AM
      Guiding pancreatic cancer therapy by individualized epigenetics
      Guiding pancreatic cancer therapy by individualized epigenetics
      Date: October 28, 2020 Time: 7:00am (PDT), 10:00am (EDT) Although chemotherapy remains the mainstay of systemic therapy, a large number of cancer patients fail to respond to it. About half o...
      Speaker: Surajit Dhara, Ph.D. , Valentina Maran, Ph.D.
      Sponsored By: Agilent
      12
    AUG 04, 2021 | 8:00 AM
    The Power of Autofluorescence Extraction Using Full Spectrum Profilingâ„¢
    The Power of Autofluorescence Extraction Using Full Spectrum Profilingâ„¢
    Date: August 4, 2021 Time: 8:00am (PDT), 11:00am (EDT) The intrinsic emission of light by biological structures in the cells, known as autofluorescence (AF), can be detrimental for data reso...
    Speaker: Diana Escobar
    Sponsored By: Cytek Biosciences
    8 1
    JUN 30, 2021 | 7:00 AM
    Optical Genome Mapping: Unrivaled Structural Variant Detection for Cancer Research and Precision Medicine
    Optical Genome Mapping: Unrivaled Structural Variant Detection...
    Date: June 30, 2021 Time: 7:00am (PDT), 8:00am (EDT) Join us to discover how Optical genome mapping (OGM) mapping can help you in the characterization of complex samples. OGM is a non-sequen...
    Speaker: Yannick Delpu, PhD , Darren "Ben" Finlay, PhD
    Sponsored By: Bionano
    8
    JUN 09, 2021 | 7:00 AM
    Preventing cell misidentification in your daily lab routine
    Preventing cell misidentification in your daily lab routine
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    Speaker: Gabriela Saldanha , Jessica Wagener, PhD
    Sponsored By: Eppendorf
    40 3
    MAY 11, 2021 | 10:00 AM
    Secure Your Next Great Discovery
    Secure Your Next Great Discovery
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    Speaker: Paulina Kocjan , Kate Meola , Zareh Zurabyan
    Presented at: Eppendorf Planet of Knowledge - A New Dimension
    Sponsored By: Eppendorf
    8
    APR 21, 2021 | 5:00 PM
    Immunology Researchers share their Spatial Discoveries in SARS-CoV-2 host response and in multiple sclerosis
    Immunology Researchers share their Spatial Discoveries in SARS-CoV-2...
    Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
    Speaker: Doug Brown, PhD , Susanne Krasemann, MD, PhD , Michelle Naughton, PhD
    Sponsored By: NanoString Technologies, Illumina
    5
     
     
  • APR 20, 2021 | 4:30 PM
    C.E. CREDITS
    High-throughput clinical and environmental viral extraction and sequencing
    High-throughput clinical and environmental viral extraction and...
    COVID-19 is a severe disease that has caused >1 million deaths in under one year. As this disease is novel, the molecular and cellular underpinnings of the progressive tissue injury are p...
    Speaker: Christopher Mason, Ph.D
    Presented at: Genetics Week Virtual Event Series 2021
    Sponsored By: Tecan
    2
    MAR 31, 2021 | 8:00 AM
    CytoFLEX SRT Cell Sorter: What is it really like for a user?
    CytoFLEX SRT Cell Sorter: What is it really like for a user?
    Date: March 31, 2021 Time: 08:00am PST CytoFLEX SRT has enabled researcher lead self-service sorting. The need for a cell sorting expert to setup and maintain the sorter status is now necess...
    Speaker: Peter O'Toole, PhD , Dr. Karen Hogg
    Sponsored By: Beckman Coulter Life Sciences
    7
    MAR 17, 2021 | 8:00 AM
    Advanced enzymes to facilitate SARS-CoV-2 research
    Advanced enzymes to facilitate SARS-CoV-2 research
    Date: March 17, 2021 Time: 08:00am (PST) Research focused on SARS-CoV-2 has become a primary focus in genomic and molecular biology fields. With a constantly changing field with new methods...
    Speaker: Li Lavelle
    Sponsored By: Thermo Fisher Scientific
    24 1
    FEB 24, 2021 | 1:30 PM
    Roundtable Panel Presentation: Recent Trends in Screening GPCRs
    Roundtable Panel Presentation: Recent Trends in Screening GPCRs
    G protein-coupled receptors (GPCRs) are among the most intensively studied drug targets, and account for about ~34% of all drugs approved by the FDA. Examples of drugs targeting GPCRs includ...
    Speaker: Annette Gilchrist, PhD , Professor Kevin Pfleger, MA, PhD, FBPhS , Juan José Fung, PhD , Aarti Kawatkar , Yamina A. Berchiche, PhD , Ajay Yekkirala, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    1
    FEB 24, 2021 | 10:00 AM
    Biosafety Enclosures and the Automated Lab: Steps for Success
    Biosafety Enclosures and the Automated Lab: Steps for Success
    DATE: February 24, 2021 TIME: 10am PST Automated lab instruments such as liquid handlers and cell sorters are increasingly common in all types of laboratories, driving fast results for labor...
    Speaker: David Wasescha , Simon Fogarty
    Sponsored By: Labconco
    15
     
     
  • FEB 24, 2021 | 12:00 AM
    Preclinical evaluation of novel OT-analogs for the potential treatment of social behavioral deficits
    Preclinical evaluation of novel OT-analogs for the potential treatment...
    As pharmaceutical companies have reduced or eliminated neuroscience drug development due to high costs and low approval rates, the economic and healthcare burden of CNS disorders in the Unit...
    Speaker: Marsha Pierce, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    2
    FEB 24, 2021 | 12:00 AM
    The development and application of TRUPATH biosensors to the study of GPCR pharmacology and functional selectivity
    The development and application of TRUPATH biosensors to the study...
    G-protein-coupled receptors (GPCRs) remain major drug targets, despite an incomplete understanding of how they signal through 16 non-visual G-protein signal transducers (collectively named t...
    Speaker: Reid Olsen, PhD, Pharmacology, Head of GPCR Pharmacology
    Presented at: Drug Discovery & Development Virtual Event Series 2021
    4
    DEC 16, 2020 | 8:00 AM
    Adding Dimensions to Multiplex Molecular Imaging
    Adding Dimensions to Multiplex Molecular Imaging
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    Speaker: Scott Fraser, PhD
    Sponsored By: Leica Microsystems
    8
    DEC 09, 2020 | 9:00 AM
    Human hematopoietic stem cell research made smart and easy
    Human hematopoietic stem cell research made smart and easy
    DATE: December 09, 2020 TIME: 09:00am PST Hematopoietic stem cells (HSCs) ensure homeostasis of blood throughout life. In this webinar, we share insights into the smart and easy isolation, c...
    Speaker: Antje Kremer
    Sponsored By: Miltenyi Biotec
    15 1
    DEC 04, 2020 | 9:00 AM
    IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody Cocktail - Visualizing immune response during infections
    IOTest Myeloid Activation CD169-PE/HLA-DR-APC/CD64-PB Antibody...
    Date: December 4, 2020 Time: 9:00am PST, 12:00pm EST Activation of Myeloid cells and interferon release is an early response to infection. The 3-marker combination (CD169/CD64/HLA-DR) gives...
    Speaker: Shankar Pattabhiraman
    Sponsored By: Beckman Coulter Life Sciences
    5
     
     
  • DEC 03, 2020 | 10:30 AM
    The Immunology of COVID-19: A dynamic immune signature for prognosis and spatial transcriptomics of lung tissue
    The Immunology of COVID-19: A dynamic immune signature for prognosis...
    The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and has spread globally, causing a pandemic of respiratory illness designated coronavirus disease 20...
    Speaker: Arutha Kulasinghe, PhD , Miguel Muñoz-Ruiz, PhD
    Presented at: Coronavirus Series
    Sponsored By: NanoString Technologies
    3
    DEC 02, 2020 | 8:00 AM
    Optimize your bioprocess - From shaker to bioreactor
    Optimize your bioprocess - From shaker to bioreactor
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    Speaker: Ines Hartmann , David Solbach
    Sponsored By: Eppendorf
    11
    NOV 19, 2020 | 9:00 AM
    From Protein Structures to Drug Discovery: Novel therapeutic opportunities.
    From Protein Structures to Drug Discovery: Novel therapeutic opportunities.
    DATE: November 19, 2020 TIME: 9:00am PT, 12am ET Knowledge of the three-dimensional structure of therapeutically relevant targets has become an essential step in the pipeline of drug discove...
    Speaker: Amal Hassan Ali
    Sponsored By: Thermo Fisher Scientific
    16 1
    NOV 19, 2020 | 9:00 AM
    Highly specific multiplexed RNA imaging in tissues with split-FISH
    Highly specific multiplexed RNA imaging in tissues with split-FISH
    Date: November 19, 2020 Time: 12:00am (PDT), 9:00am (CET), 4:00pm (SGT) We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to...
    Speaker: Kok Hao Chen , Alan Mullan, PhD
    Sponsored By: Andor
    6 2
    NOV 18, 2020 | 8:00 AM
    Whole-organ mapping using AI and light sheet microscopy
    Whole-organ mapping using AI and light sheet microscopy
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    Speaker: Ali Ertürk
    Sponsored By: Miltenyi Biotec
    14
     
     
  • NOV 18, 2020 | 7:00 AM
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis
    Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common...
    DATE: November 18, 2020 TIME: 7:00am PST, 10:00am PST, 4:00pm CEST The assessment of cell health and cellular responses after experimental manipulation continue to be a very important......
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf, Promega
    17
    NOV 16, 2020 | 8:00 AM
    Targeted single-cell RNA sequencing enables genome-scale CRISPR screens
    Targeted single-cell RNA sequencing enables genome-scale CRISPR...
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    Speaker: Wieland Keilholz, Ph.D. , Pawel Zajac, Ph.D. , Dr. Tilmann Buerckstuemmer
    Sponsored By: Illumina
    11
    NOV 05, 2020 | 7:00 AM
    Stem cell derived exosome production in stirred-tank bioreactors
    Stem cell derived exosome production in stirred-tank bioreactors
    DATE: Date needed, 2020 TIME: Time needed Exosomes are a population of naturally occurring mobile, membrane-limited, 30 – 100 nm in diameter, extracellular vesicles containing a large...
    Speaker: Jorge Escobar , Aurélie Tacheny
    Presented at: Eppendorf Planet of Knowledge - A New Dimension
    Sponsored By: Eppendorf
    12
    OCT 28, 2020 | 7:00 AM
    Guiding pancreatic cancer therapy by individualized epigenetics
    Guiding pancreatic cancer therapy by individualized epigenetics
    Date: October 28, 2020 Time: 7:00am (PDT), 10:00am (EDT) Although chemotherapy remains the mainstay of systemic therapy, a large number of cancer patients fail to respond to it. About half o...
    Speaker: Surajit Dhara, Ph.D. , Valentina Maran, Ph.D.
    Sponsored By: Agilent
    12
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