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    Genomics

    Genomics: an interdisciplinary field of science within the field of molecular biology. A genome is a complete set of DNA within a single cell of an organism, and as such, focuses on the structure, function, evolution, and mapping of genomes. Genomics aims at the collective characterization and quantification of genes, which direct the production of proteins with the assistance of enzymes and messenger molecules. Genomics also involves the sequencing and analysis of genomes.

    Webinars

    • OCT 05, 2021 | 7:00 AM
      Resolving Challenges in Flow Cytometry Panel Design
      Resolving Challenges in Flow Cytometry Panel Design
      Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...
      Speaker: Michael Kapinsky, PhD , Matthew Goff
      Sponsored By: Beckman Coulter Life Sciences
      13 1
      SEP 29, 2021 | 11:59 PM
      Nanoparticle Based Delivery of Gene Editing Enzymes
      Nanoparticle Based Delivery of Gene Editing Enzymes
      Cas9 based therapeutics have the potential to revolutionize the treatment of genetic diseases. However, safe and effective methods for delivering Cas9 protein, gRNA and donor DNA need to be...
      Speaker: Niren Murthy, PhD
      Presented at: CRISPR Virtual Event Series 2021
      2 2
      SEP 29, 2021 | 12:00 PM
      How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
      How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
      Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing...
      Speaker: Domenica Martorana, PhD
      Presented at: CRISPR Virtual Event Series 2021
      Sponsored By: QIAGEN
      1
      SEP 29, 2021 | 9:00 AM
      Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome Engineering
      Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome...
      RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 and novel RNA targeti...
      Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
      Presented at: CRISPR Virtual Event Series 2021
      1
      SEP 28, 2021 | 9:00 AM
      New method for standardized, high-throughput PBMC isolation from blood samples for observational and clinical studies
      New method for standardized, high-throughput PBMC isolation from...
      Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
      Speaker: Lotta Raty , Dr. Steffen Rapp , PhD
      Sponsored By: Miltenyi Biotec
      10 1
      SEP 23, 2021 | 10:00 AM
      From start up to scale up: Creative solutions to modernize your lab budget
      From start up to scale up: Creative solutions to modernize your...
      Date: Septembee 23, 2021 Time: 8:00am (PDT), 11:00am (EDT) Join leaders and financial experts in the biotech and clinical space to engage in discussions about overcoming financial challenges...
      Speaker: Dr. Richard Qun Shan , Jason Hansen , Travis Hardman
      Sponsored By: Thermo Fisher Scientific
      4
      SEP 22, 2021 | 7:30 AM
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
      Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
      Speaker: Jeff Hou, PhD , Steve Lewis
      Presented at: Cell Biology Virtual Event Series 2021
      Sponsored By: Thermo Fisher Scientific
      0
      SEP 16, 2021 | 8:00 AM
      Concept-to-Commercialization: Thermo Fisher's unique approach to rapidly advancing your antibody and protein therapeutics
      Concept-to-Commercialization: Thermo Fisher's unique approach...
      Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
      Speaker: Jeff Hou, PhD , Steve Lewis
      Sponsored By: Thermo Fisher Scientific
      1
      SEP 14, 2021 | 7:00 AM
      What prevents you from increasing your PCR productivity?
      What prevents you from increasing your PCR productivity?
      Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
      Speaker: Arora Phang, PhD
      Sponsored By: Eppendorf
      24 1
      SEP 09, 2021 | 10:30 AM
      Approaches to SARS-CoV-2 Sample Processing for RNA Detection
      Approaches to SARS-CoV-2 Sample Processing for RNA Detection
      The ongoing COVID-19 pandemic has elevated the need for reliable, cost-effective means for widespread virus detection. This webinar will explore two distinct approaches for isolating viral R...
      Speaker: Nicolas Caffarelli , Kan Saito, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      Sponsored By: MilliporeSigma
      5
      SEP 09, 2021 | 7:00 AM
      Maximize your variant knowledge using the Human Gene Mutation Database (HGMD®) Professional
      Maximize your variant knowledge using the Human Gene Mutation...
      Date: September 9, 2021 Time: 7:00am (PDT), 10:00am (EDT) Founded and maintained by the Institute of Medical Genetics at Cardiff University in 1996, HGMD attempts to collate all known, publi...
      Speaker: Dr. Ruth Burton
      Sponsored By: QIAGEN
      3
      SEP 08, 2021 | 12:00 AM
      Translating Pharmacomicrobiomics: From Microbiome Cloud Uncertainty to Pharmaceutical and Clinical Application(s)
      Translating Pharmacomicrobiomics: From Microbiome Cloud Uncertainty...
      The Human Microbiome Project was conceived almost 15 years ago, as an extension of the Human Genome Project, to explore the diversity of human-associated microorganisms at multiple body site...
      Speaker: Ramy K. Aziz, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      1
      SEP 08, 2021 | 12:00 AM
      Machine Learning Approaches for Modeling Complex Community Dynamics
      Machine Learning Approaches for Modeling Complex Community Dynamics
      Computational or mathematical modeling generally refers to a mechanism-based formalism that help us test hypotheses, expand our understanding of a system, or make mechanism-aware predictions...
      Speaker: Nicholas Chia, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      AUG 25, 2021 | 8:00 AM
      Improving heterologous protein production in yeast with massively parallel CRISPR genome editing
      Improving heterologous protein production in yeast with massively...
      Date: August 25, 2021 Time: 8:00am (PDT), 11:00am (EDT) Heterologous protein production is an indispensable tool in biotechnology and biopharma for manufacturing enzymes, protein therapeutic...
      Speaker: Eric Abbate
      Sponsored By: Inscripta
      5 2
      AUG 25, 2021 | 7:00 AM
      Automation-aided targeted genotyping with Allegro for maize breeding: a case study of QTL analysis with MAGIC mapping populations
      Automation-aided targeted genotyping with Allegro for maize breeding:...
      Date: August 25, 2021 Time: 7:00am PDT DreamPrep NGS is a complete solution that combines the Fluent® Automation Workstation, the Infinite® F Nano+ plate reader and Tecan Genomics&rsq...
      Speaker: Dr. Davide Scaglione , Leonardo Caproni, PhD
      Sponsored By: Tecan
      4
      AUG 25, 2021 | 12:00 AM
      Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic Lateral Sclerosis
      Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic...
      Over 40 tandem repeats undergo expansion events that lead to neurological disease. This number is likely an underestimate as many repeats are difficult to amplify using existing short read s...
      Speaker: Paul Valdmanis, PhD
      Presented at: Neuroscience Virtual Event Series 2021
      Sponsored By: PacBio
      1
      AUG 25, 2021 | 12:00 AM
      Can Multiscale Computer Modeling Save the Human Genome Project?: Climbing the Omes for Schizophrenia
      Can Multiscale Computer Modeling Save the Human Genome Project?:...
      Multiscale modeling has arisen as a focus of computational systems biology, with the realization that genome, proteome, connectome, etceteromes, will only become comprehensible once placed i...
      Speaker: William Lytton, MD
      Presented at: Neuroscience Virtual Event Series 2021
      3
      JUL 29, 2021 | 10:00 AM
      The importance of simultaneously detecting and differentiating SARS-CoV-2 and influenza during flu season
      The importance of simultaneously detecting and differentiating...
      Date: July 29, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Experts are predicting an increase in influenza cases and a constant presence of SARS-CoV-2 in the shortly approaching influenza season....
      Speaker: Peter Friebe, Ph.D. , Jelena Feenstra, PhD
      Sponsored By: Thermo Fisher Scientific
      12 1
      JUL 29, 2021 | 9:00 AM
      Expanded Carrier Screening Research in the Genomic Era
      Expanded Carrier Screening Research in the Genomic Era
      Date: July 29, 2021 Time: 1:00am (PDT), 4:00am (EDT) Traditionally limited to a small number of phenotypes and mutations suggested to specific risk groups, the emerging field of genomics i...
      Speaker: Dr. Doron Behar, MD, PhD
      Sponsored By: Thermo Fisher Scientific
      7
      JUL 28, 2021 | 8:00 AM
      Disease model generation: 5 steps to a 3D cancer spheroid model
      Disease model generation: 5 steps to a 3D cancer spheroid model
      Date: July 28, 2021 Time: 8:00am PDT Breast cancer is the most common form of cancer in women. It is estimated that 1 out of 8 women in the US will develop invasive breast cancer during her...
      Speaker: Edwin Golez, M.S.
      Sponsored By: Thermo Fisher Scientific
      9
      JUL 15, 2021 | 9:00 AM
      Veranome Webinar Series:  Highly Multiplexed Analysis of Mouse Neural Tissues
      Veranome Webinar Series: Highly Multiplexed Analysis of Mouse...
      Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
      Speaker: Brian Hilbush, Ph.D.
      Sponsored By: Veranome Biosystems
      9
      JUL 13, 2021 | 11:00 AM
      Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow
      Monoclonality Assurance Using CloneSelect Single-Cell Printer...
      Date: July 13, 2021 Time: 11:00am (PDT), 1:00pm (EDT) 1 R&D, Cell Culture & Fermentation Sciences, Bio Pharmaceutical Development, AstraZeneca, Gaithersburg, USA, 2 R&D, ADPE, Ce...
      Speaker: Gargi Roy
      Sponsored By: Molecular Devices
      9 10
      JUL 01, 2021 | 8:00 AM
      Quality control advancements in therapeutic genome editing and biomanufacturing
      Quality control advancements in therapeutic genome editing and...
      Date: July 1, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) What’s driving precision-medicine innovations? New advances in gene and cell therapy manufacturing, along with more...
      Speaker: Imen Mestiri, PhD
      Sponsored By: Genomic Vision
      9
      JUN 30, 2021 | 11:00 AM
      How community hospital labs can perform comprehensive genomic profiling with the support of professional clinical interpretation services
      How community hospital labs can perform comprehensive genomic...
      Date: June 30, 2021 Time: 11:00am PDT As next-generation DNA sequencing technology continues to evolve and become further integrated into routine clinical testing, the need for efficient and...
      Speaker: Sheryl Elkin, PhD , Linda Call
      Sponsored By: QIAGEN
      3
    OCT 05, 2021 | 7:00 AM
    Resolving Challenges in Flow Cytometry Panel Design
    Resolving Challenges in Flow Cytometry Panel Design
    Date: October 05, 2021 Time: 7:00am (PDT), 10:00am (EDT) After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next...
    Speaker: Michael Kapinsky, PhD , Matthew Goff
    Sponsored By: Beckman Coulter Life Sciences
    13 1
    SEP 29, 2021 | 11:59 PM
    Nanoparticle Based Delivery of Gene Editing Enzymes
    Nanoparticle Based Delivery of Gene Editing Enzymes
    Cas9 based therapeutics have the potential to revolutionize the treatment of genetic diseases. However, safe and effective methods for delivering Cas9 protein, gRNA and donor DNA need to be...
    Speaker: Niren Murthy, PhD
    Presented at: CRISPR Virtual Event Series 2021
    2 2
    SEP 29, 2021 | 12:00 PM
    How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
    How Can You Characterize Your CRISPR Gene Edits Faster and Easier?
    Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing...
    Speaker: Domenica Martorana, PhD
    Presented at: CRISPR Virtual Event Series 2021
    Sponsored By: QIAGEN
    1
    SEP 29, 2021 | 9:00 AM
    Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome Engineering
    Panel Presentation: Harnessing Novel CRISPR Systems for Transcriptome...
    RNA plays important and diverse roles in biology, but molecular tools to manipulate and measure RNA are limited. We demonstrate that RNA-targeting CRISPR effector Cas13 and novel RNA targeti...
    Speaker: Omar Abudayyeh, PhD , Jonathan Gootenberg, PhD
    Presented at: CRISPR Virtual Event Series 2021
    1
    SEP 28, 2021 | 9:00 AM
    New method for standardized, high-throughput PBMC isolation from blood samples for observational and clinical studies
    New method for standardized, high-throughput PBMC isolation from...
    Date: September 28, 2021 Time: 9:00am (PDT), 12:00pm (EDT) Standardization and reproducibility are prerequisites for peripheral blood mononuclear cell (PBMC) generation from donor or patient...
    Speaker: Lotta Raty , Dr. Steffen Rapp , PhD
    Sponsored By: Miltenyi Biotec
    10 1
     
     
  • SEP 23, 2021 | 10:00 AM
    From start up to scale up: Creative solutions to modernize your lab budget
    From start up to scale up: Creative solutions to modernize your...
    Date: Septembee 23, 2021 Time: 8:00am (PDT), 11:00am (EDT) Join leaders and financial experts in the biotech and clinical space to engage in discussions about overcoming financial challenges...
    Speaker: Dr. Richard Qun Shan , Jason Hansen , Travis Hardman
    Sponsored By: Thermo Fisher Scientific
    4
    SEP 22, 2021 | 7:30 AM
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
    Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
    Speaker: Jeff Hou, PhD , Steve Lewis
    Presented at: Cell Biology Virtual Event Series 2021
    Sponsored By: Thermo Fisher Scientific
    0
    SEP 16, 2021 | 8:00 AM
    Concept-to-Commercialization: Thermo Fisher's unique approach to rapidly advancing your antibody and protein therapeutics
    Concept-to-Commercialization: Thermo Fisher's unique approach...
    Date: September 16, 2021 Time: 8:00am (PDT), 11:00am (EDT) Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. T...
    Speaker: Jeff Hou, PhD , Steve Lewis
    Sponsored By: Thermo Fisher Scientific
    1
    SEP 14, 2021 | 7:00 AM
    What prevents you from increasing your PCR productivity?
    What prevents you from increasing your PCR productivity?
    Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
    Speaker: Arora Phang, PhD
    Sponsored By: Eppendorf
    24 1
    SEP 09, 2021 | 10:30 AM
    Approaches to SARS-CoV-2 Sample Processing for RNA Detection
    Approaches to SARS-CoV-2 Sample Processing for RNA Detection
    The ongoing COVID-19 pandemic has elevated the need for reliable, cost-effective means for widespread virus detection. This webinar will explore two distinct approaches for isolating viral R...
    Speaker: Nicolas Caffarelli , Kan Saito, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    Sponsored By: MilliporeSigma
    5
     
     
  • SEP 09, 2021 | 7:00 AM
    Maximize your variant knowledge using the Human Gene Mutation Database (HGMD®) Professional
    Maximize your variant knowledge using the Human Gene Mutation...
    Date: September 9, 2021 Time: 7:00am (PDT), 10:00am (EDT) Founded and maintained by the Institute of Medical Genetics at Cardiff University in 1996, HGMD attempts to collate all known, publi...
    Speaker: Dr. Ruth Burton
    Sponsored By: QIAGEN
    3
    SEP 08, 2021 | 12:00 AM
    Translating Pharmacomicrobiomics: From Microbiome Cloud Uncertainty to Pharmaceutical and Clinical Application(s)
    Translating Pharmacomicrobiomics: From Microbiome Cloud Uncertainty...
    The Human Microbiome Project was conceived almost 15 years ago, as an extension of the Human Genome Project, to explore the diversity of human-associated microorganisms at multiple body site...
    Speaker: Ramy K. Aziz, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    1
    SEP 08, 2021 | 12:00 AM
    Machine Learning Approaches for Modeling Complex Community Dynamics
    Machine Learning Approaches for Modeling Complex Community Dynamics
    Computational or mathematical modeling generally refers to a mechanism-based formalism that help us test hypotheses, expand our understanding of a system, or make mechanism-aware predictions...
    Speaker: Nicholas Chia, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
    AUG 25, 2021 | 8:00 AM
    Improving heterologous protein production in yeast with massively parallel CRISPR genome editing
    Improving heterologous protein production in yeast with massively...
    Date: August 25, 2021 Time: 8:00am (PDT), 11:00am (EDT) Heterologous protein production is an indispensable tool in biotechnology and biopharma for manufacturing enzymes, protein therapeutic...
    Speaker: Eric Abbate
    Sponsored By: Inscripta
    5 2
    AUG 25, 2021 | 7:00 AM
    Automation-aided targeted genotyping with Allegro for maize breeding: a case study of QTL analysis with MAGIC mapping populations
    Automation-aided targeted genotyping with Allegro for maize breeding:...
    Date: August 25, 2021 Time: 7:00am PDT DreamPrep NGS is a complete solution that combines the Fluent® Automation Workstation, the Infinite® F Nano+ plate reader and Tecan Genomics&rsq...
    Speaker: Dr. Davide Scaglione , Leonardo Caproni, PhD
    Sponsored By: Tecan
    4
     
     
  • AUG 25, 2021 | 12:00 AM
    Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic Lateral Sclerosis
    Mechanistic Insight into Tandem Repeat Expansions in Amyotrophic...
    Over 40 tandem repeats undergo expansion events that lead to neurological disease. This number is likely an underestimate as many repeats are difficult to amplify using existing short read s...
    Speaker: Paul Valdmanis, PhD
    Presented at: Neuroscience Virtual Event Series 2021
    Sponsored By: PacBio
    1
    AUG 25, 2021 | 12:00 AM
    Can Multiscale Computer Modeling Save the Human Genome Project?: Climbing the Omes for Schizophrenia
    Can Multiscale Computer Modeling Save the Human Genome Project?:...
    Multiscale modeling has arisen as a focus of computational systems biology, with the realization that genome, proteome, connectome, etceteromes, will only become comprehensible once placed i...
    Speaker: William Lytton, MD
    Presented at: Neuroscience Virtual Event Series 2021
    3
    JUL 29, 2021 | 10:00 AM
    The importance of simultaneously detecting and differentiating SARS-CoV-2 and influenza during flu season
    The importance of simultaneously detecting and differentiating...
    Date: July 29, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Experts are predicting an increase in influenza cases and a constant presence of SARS-CoV-2 in the shortly approaching influenza season....
    Speaker: Peter Friebe, Ph.D. , Jelena Feenstra, PhD
    Sponsored By: Thermo Fisher Scientific
    12 1
    JUL 29, 2021 | 9:00 AM
    Expanded Carrier Screening Research in the Genomic Era
    Expanded Carrier Screening Research in the Genomic Era
    Date: July 29, 2021 Time: 1:00am (PDT), 4:00am (EDT) Traditionally limited to a small number of phenotypes and mutations suggested to specific risk groups, the emerging field of genomics i...
    Speaker: Dr. Doron Behar, MD, PhD
    Sponsored By: Thermo Fisher Scientific
    7
    JUL 28, 2021 | 8:00 AM
    Disease model generation: 5 steps to a 3D cancer spheroid model
    Disease model generation: 5 steps to a 3D cancer spheroid model
    Date: July 28, 2021 Time: 8:00am PDT Breast cancer is the most common form of cancer in women. It is estimated that 1 out of 8 women in the US will develop invasive breast cancer during her...
    Speaker: Edwin Golez, M.S.
    Sponsored By: Thermo Fisher Scientific
    9
     
     
  • JUL 15, 2021 | 9:00 AM
    Veranome Webinar Series:  Highly Multiplexed Analysis of Mouse Neural Tissues
    Veranome Webinar Series: Highly Multiplexed Analysis of Mouse...
    Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
    Speaker: Brian Hilbush, Ph.D.
    Sponsored By: Veranome Biosystems
    9
    JUL 13, 2021 | 11:00 AM
    Monoclonality Assurance Using CloneSelect Single-Cell Printer and CloneSelect Imager in Human Stem Cell (hSC) Engineering Workflow
    Monoclonality Assurance Using CloneSelect Single-Cell Printer...
    Date: July 13, 2021 Time: 11:00am (PDT), 1:00pm (EDT) 1 R&D, Cell Culture & Fermentation Sciences, Bio Pharmaceutical Development, AstraZeneca, Gaithersburg, USA, 2 R&D, ADPE, Ce...
    Speaker: Gargi Roy
    Sponsored By: Molecular Devices
    9 10
    JUL 01, 2021 | 8:00 AM
    Quality control advancements in therapeutic genome editing and biomanufacturing
    Quality control advancements in therapeutic genome editing and...
    Date: July 1, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) What’s driving precision-medicine innovations? New advances in gene and cell therapy manufacturing, along with more...
    Speaker: Imen Mestiri, PhD
    Sponsored By: Genomic Vision
    9
    JUN 30, 2021 | 11:00 AM
    How community hospital labs can perform comprehensive genomic profiling with the support of professional clinical interpretation services
    How community hospital labs can perform comprehensive genomic...
    Date: June 30, 2021 Time: 11:00am PDT As next-generation DNA sequencing technology continues to evolve and become further integrated into routine clinical testing, the need for efficient and...
    Speaker: Sheryl Elkin, PhD , Linda Call
    Sponsored By: QIAGEN
    3
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