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    Recombinant Protein Expression

    Recombinant Protein Expression: Recombinant Protein is a protein encoded by a gene — recombinant DNA — that has been cloned in a system that supports expression of the gene and translation of messenger RNA (see expression system). ... Proteins that result from the expression of recombinant DNA within living cells are termed recombinant proteins.

    Webinars

    • SEP 25, 2019 | 8:00 AM
      Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems:  Case study of Potassium chloride co-transporter KCC2
      Expression, Purification and Characterization of Difficult to...
      DATE: September 25, 2019TIME: 8:00am PT, 11:00am ET This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibco™ Expi293™ and ExpiCHO™ Expres...
      Speaker: Anass Jawhari, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      4
      SEP 12, 2019 | 1:30 PM
      Gender Parity in Virology Conference Speaker Selections
      Gender Parity in Virology Conference Speaker Selections
      Scientific conferences are most beneficial to participants when they showcase significant new experimental developments, accurately summarize the current state of the field, and provide stron...
      Speaker: Ann Palmenberg, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2019
      1
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
      AUG 29, 2019 | 9:00 AM
      Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
      Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
      DATE: August 29, 2019TIME: 9:00am PT/ 12:00pm ET Monoclonal antibodies (mAbs) are a cornerstone of modern biomedical research and biotherapeutics. Therefore, reproducibly obtai...
      Speaker: Ian Huggins, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      JAN 31, 2019 | 6:00 AM
      Overcoming Challenges in EuCODE Cell Culture Platform Development
      Overcoming Challenges in EuCODE Cell Culture Platform Development
      Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the...
      Speaker: Weimin Lin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      3
      SEP 18, 2018 | 8:00 AM
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
      Speaker: Judd Hultquist, PhD
      Sponsored By: Horizon Discovery
      7
      JUN 27, 2018 | 8:00 AM
      Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize success
      Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize...
      DATE: June 27, 2018TIME: 08:00AM PDT CRISPR-Cas9 is a very versatile tool to discover more about your pathway or gene of interest. Its simplicity has led to an explosion in a...
      Speaker: Laura Carleton, PhD
      Sponsored By: Horizon Discovery
      13 1
      MAY 17, 2018 | 10:00 AM
      A comprehensive workflow solution from gene synthesis to purified proteins
      A comprehensive workflow solution from gene synthesis to purified...
      Reliable expression of recombinant proteins in human or CHO cells is essential for many aspects of biomedical research and drug development but is often hampered by low expression yields that...
      Speaker: Michael Liss, PhD
      Presented at: Gibco ExpressionWorld 2018
      5
      MAY 17, 2018 | 9:00 AM
      Efficient purification of recombinant Strep-tag® fusion proteins from Expi supernatants using Strep-Tactin®XT
      Efficient purification of recombinant Strep-tag® fusion proteins...
      Mammalian transient expression has become an effective method to provide recombinant proteins in a short time. Therefore, mammalian transient expression systems are applied to a great extend...
      Speaker: Dennis Niermeier, MS
      Presented at: Gibco ExpressionWorld 2018
      5
      MAY 17, 2018 | 7:00 AM
      A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells
      A Chemically-Defined Baculovirus-Based Expression System for Enhanced...
      The Baculovirus Expression Vector System (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to seru...
      Speaker: Maya Yovcheva
      Presented at: Gibco ExpressionWorld 2018
      5
      MAY 17, 2018 | 6:00 AM
      Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems
      Strategies for High-Titer Protein Expression Using the ExpiCHO...
      The ExpiCHO and Expi293 transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate d...
      Speaker: Jon Zmuda, PhD
      Presented at: Gibco ExpressionWorld 2018
      6
      MAY 16, 2018 | 11:00 PM
      A chemically defined baculovirus-based expression system for enhanced protein production in Sf9 cells (presented in Chinese)
      A chemically defined baculovirus-based expression system for enhanced...
      摘要杆状病毒表达载体系统(BEVS)是重组蛋白生产的主要平台之一。与哺乳动物表达系统早已转换为无血清,化学成分限定的培养基不同,昆虫表达系统的创新相对较少。昆虫细胞继续依赖成分不限定的含酵母提取物的培养基,而这些培养基可以显示出明显的细胞生长和蛋白质表达的批次差异。在这里,我们介绍一种新型的Sf9细胞杆状病毒表达系统的开发。此系统包括支持高细胞密度、化学成分限定的培养基,...
      Speaker: Nan Yang
      Presented at: Gibco ExpressionWorld 2018
      3
      MAY 16, 2018 | 7:00 PM
      Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems (presented in Chinese)
      Strategies for High-Titer Protein Expression Using the ExpiCHO...
      Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统,目前已广泛应用于治疗性候选药物的早期鉴定,诊断性试剂的快速生产,以及疫苗研发、结构生物学和膜蛋白生物学等研究领域,成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达,但HEK293 细胞和 CHO 细胞之间存在的内在...
      Speaker: Chao Yan Liu
      Presented at: Gibco ExpressionWorld 2018
      6 2
      FEB 21, 2018 | 12:00 PM
      Allosteric Modulation of Metabotropic Glutamate Receptors for the Treatment of Neurodevelopmental Disorders
      Allosteric Modulation of Metabotropic Glutamate Receptors for...
      Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the Methyl CpG Binding Protein 2 (MECP2) gene. In mouse models of RTT, deficits in long-term potentiation (LTP) at...
      Speaker: Colleen Niswender, PhD
      Presented at: Drug Discovery Virtual Event Series 2018
      1
      SEP 28, 2017 | 12:00 PM
      Back to basics: Fundamental concepts and special considerations in RNA isolation
      Back to basics: Fundamental concepts and special considerations...
      How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
      Speaker: Abhishek Sharma
      Presented at: Cell Biology Virtual Event Series 2017
      2
      MAY 10, 2017 | 2:00 PM
      Selecting the right resin to achieve optimal results, from high throughput batch to process scale protein purification
      Selecting the right resin to achieve optimal results, from high...
      The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
      Speaker: Aaron McBride , Scott Meier
      Presented at: 2017 Gibco ExpressionWorld
      7
      MAY 10, 2017 | 12:00 PM
      Ask the Expert: Mammalian Transient Expression
      Ask the Expert: Mammalian Transient Expression
      Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
      Speaker: Henry Chiou, PhD , Richard Altman, MS , Jon Zmuda, PhD , Konrad Miatkowski
      Presented at: 2017 Gibco ExpressionWorld
      3
      MAY 10, 2017 | 11:00 AM
      An ongoing evaluation of our transient expression systems; Can we predict the appropriate expression system for any drug modality?
      An ongoing evaluation of our transient expression systems; Can...
      CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells.  The ability to e...
      Speaker: Richard Altman, MS
      Presented at: 2017 Gibco ExpressionWorld
      4
      MAY 10, 2017 | 6:00 AM
      Latest Developments in High Titer Transient Protein Expression using the ExpiCHO Transient Expression System
      Latest Developments in High Titer Transient Protein Expression...
      The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alt...
      Speaker: Jon Zmuda, PhD
      Presented at: 2017 Gibco ExpressionWorld
      8
      MAY 10, 2017 | 5:00 AM
      Complete workflow solution from gene-to-protein combine highly advanced expression systems with expression optimized genes
      Complete workflow solution from gene-to-protein combine highly...
      Reliable autologous expression of recombinant human proteins - from gene sequence to the expressed and purified protein - in human or CHO cells is essential for many aspects of biomedical res...
      Speaker: Stephan Fath, PhD
      Presented at: 2017 Gibco ExpressionWorld
      4 1
      FEB 20, 2017 | 8:00 AM
      SPR - Companion from bench to bedside
      SPR - Companion from bench to bedside
      SPR technology is being improved steadily and today is an essential component in the biopharmaceutical industry. As SPR based assays extract valuable and reliable information at high accuracy...
      Speaker: Markus Sack, MSc
      Presented at: Protein Research Live
      5
      MAY 04, 2016 | 12:00 PM
      Development of a Mammalian Recombinant Protein Production Suite
      Development of a Mammalian Recombinant Protein Production Suite
      Complex recombinant protein biologics, such as monoclonal antibodies and coagulation factors, are key components of today’s biopharmaceutical industry. There are many effective paths fo...
      Speaker: Catherine Owczarek, PhD
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAY 04, 2016 | 10:00 AM
      The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification
      The ExpiCHO transient expression system: Optimization of monoclonal...
      The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system,...
      Speaker: Lina Mpanda, MSc.
      Presented at: 2016 Gibco ExpressionWorld
      2
      MAY 04, 2016 | 8:00 AM
      Transient Expression: High Cell Density Culture for Increased Protein Production
      Transient Expression: High Cell Density Culture for Increased...
      The talk will focus on the recent advances in transient protein production using high cell density culture e.g. ExpiCHO, Expi293 for supporting pre-clinical and clinical studies including rob...
      Speaker: Puneet Khandelwal, PhD
      Presented at: 2016 Gibco ExpressionWorld
      2
    SEP 25, 2019 | 8:00 AM
    Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems:  Case study of Potassium chloride co-transporter KCC2
    Expression, Purification and Characterization of Difficult to...
    DATE: September 25, 2019TIME: 8:00am PT, 11:00am ET This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibco™ Expi293™ and ExpiCHO™ Expres...
    Speaker: Anass Jawhari, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    4
    SEP 12, 2019 | 1:30 PM
    Gender Parity in Virology Conference Speaker Selections
    Gender Parity in Virology Conference Speaker Selections
    Scientific conferences are most beneficial to participants when they showcase significant new experimental developments, accurately summarize the current state of the field, and provide stron...
    Speaker: Ann Palmenberg, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2019
    1
    SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
    17 1
    AUG 29, 2019 | 9:00 AM
    Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
    Enabling Next-Generation Targeted siRNA Therapies with ExpiCHO
    DATE: August 29, 2019TIME: 9:00am PT/ 12:00pm ET Monoclonal antibodies (mAbs) are a cornerstone of modern biomedical research and biotherapeutics. Therefore, reproducibly obtai...
    Speaker: Ian Huggins, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
    JAN 31, 2019 | 6:00 AM
    Overcoming Challenges in EuCODE Cell Culture Platform Development
    Overcoming Challenges in EuCODE Cell Culture Platform Development
    Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the...
    Speaker: Weimin Lin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    3
     
     
  • SEP 18, 2018 | 8:00 AM
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
    Speaker: Judd Hultquist, PhD
    Sponsored By: Horizon Discovery
    7
    JUN 27, 2018 | 8:00 AM
    Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize success
    Cell line engineering with CRISPR-Cas9 - Tips and tricks to maximize...
    DATE: June 27, 2018TIME: 08:00AM PDT CRISPR-Cas9 is a very versatile tool to discover more about your pathway or gene of interest. Its simplicity has led to an explosion in a...
    Speaker: Laura Carleton, PhD
    Sponsored By: Horizon Discovery
    13 1
    MAY 17, 2018 | 10:00 AM
    A comprehensive workflow solution from gene synthesis to purified proteins
    A comprehensive workflow solution from gene synthesis to purified...
    Reliable expression of recombinant proteins in human or CHO cells is essential for many aspects of biomedical research and drug development but is often hampered by low expression yields that...
    Speaker: Michael Liss, PhD
    Presented at: Gibco ExpressionWorld 2018
    5
    MAY 17, 2018 | 9:00 AM
    Efficient purification of recombinant Strep-tag® fusion proteins from Expi supernatants using Strep-Tactin®XT
    Efficient purification of recombinant Strep-tag® fusion proteins...
    Mammalian transient expression has become an effective method to provide recombinant proteins in a short time. Therefore, mammalian transient expression systems are applied to a great extend...
    Speaker: Dennis Niermeier, MS
    Presented at: Gibco ExpressionWorld 2018
    5
    MAY 17, 2018 | 7:00 AM
    A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells
    A Chemically-Defined Baculovirus-Based Expression System for Enhanced...
    The Baculovirus Expression Vector System (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to seru...
    Speaker: Maya Yovcheva
    Presented at: Gibco ExpressionWorld 2018
    5
     
     
  • MAY 17, 2018 | 6:00 AM
    Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems
    Strategies for High-Titer Protein Expression Using the ExpiCHO...
    The ExpiCHO and Expi293 transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate d...
    Speaker: Jon Zmuda, PhD
    Presented at: Gibco ExpressionWorld 2018
    6
    MAY 16, 2018 | 11:00 PM
    A chemically defined baculovirus-based expression system for enhanced protein production in Sf9 cells (presented in Chinese)
    A chemically defined baculovirus-based expression system for enhanced...
    摘要杆状病毒表达载体系统(BEVS)是重组蛋白生产的主要平台之一。与哺乳动物表达系统早已转换为无血清,化学成分限定的培养基不同,昆虫表达系统的创新相对较少。昆虫细胞继续依赖成分不限定的含酵母提取物的培养基,而这些培养基可以显示出明显的细胞生长和蛋白质表达的批次差异。在这里,我们介绍一种新型的Sf9细胞杆状病毒表达系统的开发。此系统包括支持高细胞密度、化学成分限定的培养基,...
    Speaker: Nan Yang
    Presented at: Gibco ExpressionWorld 2018
    3
    MAY 16, 2018 | 7:00 PM
    Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems (presented in Chinese)
    Strategies for High-Titer Protein Expression Using the ExpiCHO...
    Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统,目前已广泛应用于治疗性候选药物的早期鉴定,诊断性试剂的快速生产,以及疫苗研发、结构生物学和膜蛋白生物学等研究领域,成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达,但HEK293 细胞和 CHO 细胞之间存在的内在...
    Speaker: Chao Yan Liu
    Presented at: Gibco ExpressionWorld 2018
    6 2
    FEB 21, 2018 | 12:00 PM
    Allosteric Modulation of Metabotropic Glutamate Receptors for the Treatment of Neurodevelopmental Disorders
    Allosteric Modulation of Metabotropic Glutamate Receptors for...
    Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the Methyl CpG Binding Protein 2 (MECP2) gene. In mouse models of RTT, deficits in long-term potentiation (LTP) at...
    Speaker: Colleen Niswender, PhD
    Presented at: Drug Discovery Virtual Event Series 2018
    1
    SEP 28, 2017 | 12:00 PM
    Back to basics: Fundamental concepts and special considerations in RNA isolation
    Back to basics: Fundamental concepts and special considerations...
    How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: &b...
    Speaker: Abhishek Sharma
    Presented at: Cell Biology Virtual Event Series 2017
    2
     
     
  • MAY 10, 2017 | 2:00 PM
    Selecting the right resin to achieve optimal results, from high throughput batch to process scale protein purification
    Selecting the right resin to achieve optimal results, from high...
    The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
    Speaker: Aaron McBride , Scott Meier
    Presented at: 2017 Gibco ExpressionWorld
    7
    MAY 10, 2017 | 12:00 PM
    Ask the Expert: Mammalian Transient Expression
    Ask the Expert: Mammalian Transient Expression
    Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
    Speaker: Henry Chiou, PhD , Richard Altman, MS , Jon Zmuda, PhD , Konrad Miatkowski
    Presented at: 2017 Gibco ExpressionWorld
    3
    MAY 10, 2017 | 11:00 AM
    An ongoing evaluation of our transient expression systems; Can we predict the appropriate expression system for any drug modality?
    An ongoing evaluation of our transient expression systems; Can...
    CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells.  The ability to e...
    Speaker: Richard Altman, MS
    Presented at: 2017 Gibco ExpressionWorld
    4
    MAY 10, 2017 | 6:00 AM
    Latest Developments in High Titer Transient Protein Expression using the ExpiCHO Transient Expression System
    Latest Developments in High Titer Transient Protein Expression...
    The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alt...
    Speaker: Jon Zmuda, PhD
    Presented at: 2017 Gibco ExpressionWorld
    8
    MAY 10, 2017 | 5:00 AM
    Complete workflow solution from gene-to-protein combine highly advanced expression systems with expression optimized genes
    Complete workflow solution from gene-to-protein combine highly...
    Reliable autologous expression of recombinant human proteins - from gene sequence to the expressed and purified protein - in human or CHO cells is essential for many aspects of biomedical res...
    Speaker: Stephan Fath, PhD
    Presented at: 2017 Gibco ExpressionWorld
    4 1
     
     
  • FEB 20, 2017 | 8:00 AM
    SPR - Companion from bench to bedside
    SPR - Companion from bench to bedside
    SPR technology is being improved steadily and today is an essential component in the biopharmaceutical industry. As SPR based assays extract valuable and reliable information at high accuracy...
    Speaker: Markus Sack, MSc
    Presented at: Protein Research Live
    5
    MAY 04, 2016 | 12:00 PM
    Development of a Mammalian Recombinant Protein Production Suite
    Development of a Mammalian Recombinant Protein Production Suite
    Complex recombinant protein biologics, such as monoclonal antibodies and coagulation factors, are key components of today’s biopharmaceutical industry. There are many effective paths fo...
    Speaker: Catherine Owczarek, PhD
    Presented at: 2016 Gibco ExpressionWorld
    2
    MAY 04, 2016 | 10:00 AM
    The ExpiCHO transient expression system: Optimization of monoclonal antibody protein A purification
    The ExpiCHO transient expression system: Optimization of monoclonal...
    The ExpiCHO transient expression system allows for the high titer production of a broad range of recombinant proteins. Due to the high titers observed for many proteins in the ExpiCHO system,...
    Speaker: Lina Mpanda, MSc.
    Presented at: 2016 Gibco ExpressionWorld
    2
    MAY 04, 2016 | 8:00 AM
    Transient Expression: High Cell Density Culture for Increased Protein Production
    Transient Expression: High Cell Density Culture for Increased...
    The talk will focus on the recent advances in transient protein production using high cell density culture e.g. ExpiCHO, Expi293 for supporting pre-clinical and clinical studies including rob...
    Speaker: Puneet Khandelwal, PhD
    Presented at: 2016 Gibco ExpressionWorld
    2
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