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    Cell Line Development

    Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.

    Webinars

    • JUL 01, 2017 | 8:00 AM
      Journal publishing tools that make YOUR research stand out
      Journal publishing tools that make YOUR research stand out
      There are a number of free and independent scholarly publishing tools available that save journal authors time and ensure research is found, read and tracked. In this talk, we will explore th...
      Speaker: Rebecca Shumbata
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      1
      JUL 01, 2017 | 8:00 AM
      Braingineering's human midbrain organoids as novel model for neurodegenerative diseases
      Braingineering's human midbrain organoids as novel model for neurodegenerative...
      Research on human brain development and neurological diseases is limited by the lack of advanced experimental in vitro models that truly recapitulate the complexity of the human brain. Here, ...
      Speaker: Jens Schwamborn
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      Pluripotent stem cells to explore mechanisms and treatments of neuromuscular diseases
      Pluripotent stem cells to explore mechanisms and treatments of...
      Disease-specific human pluripotent stem cells (hPSCs) represent a new chance to unravel cellular and molecular mechanisms of neurological diseases. Along this line, we were among the first to...
      Speaker: Cécile Martinat
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      Comparison of the Teratoma assay and in vitro surrogate tests for assessment of pluripotency of human pluripotent stem cells
      Comparison of the Teratoma assay and in vitro surrogate tests...
      For optimal use of human induced pluripotent stem cells (hiPSCs) it is essential to identify lines that are fully reprogrammed and of high quality with proven pluripotency in terms of differe...
      Speaker: Christian Freund
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      Physiological Oxygen: Historical and Molecular Perspective for Stem Cell Culture
      Physiological Oxygen: Historical and Molecular Perspective for...
      For many decades, cell lines have been cultured in standard CO2 incubators at “normal” atmospheric oxygen concentrations of about 21%. But oxygen concentrations in the human or an...
      Speaker: Mary Kay Bates, M.S.
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      1
      JUL 01, 2017 | 12:00 AM
      New Gibco B-27 Plus Neuronal Culture System-Next Generation Media for Superior Neuronal Survival and Functionality
      New Gibco B-27 Plus Neuronal Culture System-Next Generation Media...
      Since 1993, Gibco™ B-27™ Supplement and Neurobasal™ Medium has been the trusted standard for a variety of neuronal culture applications, with citations in more than 11,000 p...
      Speaker: Navjot Kaur, PhD
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      1
      JUL 01, 2017 | 12:00 AM
      Highly efficient stem cell engineering via Cas9 protein transfection
      Highly efficient stem cell engineering via Cas9 protein transfection
      CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering.  The delivery of Cas9 plasmid DNA or mR...
      Speaker: Xiquan Liang
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      New Tools for improved CRISPR gene-editing in stem cells
      New Tools for improved CRISPR gene-editing in stem cells
      The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
      Speaker: James Kehler VMD, PhD
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      2
      JUL 01, 2017 | 12:00 AM
      Comprehensive Characterization of Pluripotent Stem Cells
      Comprehensive Characterization of Pluripotent Stem Cells
      As reprogramming methodologies have become more reliable and efficient, corresponding improvements were needed in the characterization workflow as well; specifically in terms of the speed, ef...
      Speaker: Suman Pradhan
      Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
      1
      JUN 28, 2017 | 10:30 AM
      WEBINAR: Variant detection in cell-free DNA from NSCLC using NGS
      WEBINAR: Variant detection in cell-free DNA from NSCLC using NGS
      DATE: June 28, 2017TIME: 10:30am PT, 1:30pm ETThis webinar will describe the implementation of the Oncomine Lung cfDNA Assay from Thermo Fisher Scientific in the Advanced Molecular...
      Speaker: Tracy Stockley PhD, FCCMG, FACMG
      Sponsored By: Thermo Fisher Scientific - Ion Torrent
      6 1
      JUN 28, 2017 | 7:00 AM
      Analytical methods to measure empty and full AAV particles
      Analytical methods to measure empty and full AAV particles
      DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
      Speaker: Christine LeBec, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences
      9 2
      JUN 27, 2017 | 6:00 PM
      案例研究:扩大细胞治疗生产规模
      案例研究:扩大细胞治疗生产规模
      不好意思,由于遇到技术困难,此网上讨论会延迟到六月二十八日。若此时间对您不方便,您将可事后从网站上电阅。DATE: June 28, 2017 TIME: 9:00 AM HKTORDATE: June 27, 2017TIME: 6:00 PM PDT 深入了解使用一个功能性密闭状态的自动化细胞培养流...
      Speaker: Jim Beltzer, MS, PhD
      Sponsored By: TERUMO BCT
      4
      JUN 21, 2017 | 7:30 AM
      Five steps for publication-quality fixed-cell imaging the first time
      Five steps for publication-quality fixed-cell imaging the first...
      DATE: June 21, 2017TIME: 7:30am PT, 10:30am ET, 4:30pm CETChoosing the right path to discovery can hasten your success, but the wrong path can lead to missteps that extend the journey a...
      Speaker: Jason Kilgore
      Presented at: Protein and Cell Analysis Education Series
      Sponsored By: Thermo Fisher Scientific, Vert Clinical Leader
      12 1
      JUN 13, 2017 | 9:00 AM
      WEBINAR: High content imaging and analysis for drug discovery: phenotypic assays, instrumentation and software
      WEBINAR: High content imaging and analysis for drug discovery:...
      EVENT DETAILS: DATE: June 13, 2017TIME: 9:00am PT, 12:00pm ETThermo Fisher Scientific is proud to present the SyncD3 webinar series.  As a thought-leader in...
      Speaker: Nicholas Dolman, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      3
      JUN 08, 2017 | 12:00 PM
      Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing the Latest Technologies from Thermo Fisher Scientific
      Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing...
      The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
      Speaker: Rhonda Newman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      3
      JUN 08, 2017 | 10:00 AM
      WEBINAR: Analysis of the transcriptome of carriers of pathological variants in PSEN1, PSEN2 and APP that cause Alzheimer's Disease
      WEBINAR: Analysis of the transcriptome of carriers of pathological...
      DATE: June 8, 2017TIME: 10:00AM PDT, 1:00PM EDT, 7:00PM CESTAlzheimer’s Disease (AD) is the result of complex interactions between risk factors that cause pleiotropic changes in m...
      Speaker: Oscar Harari, PhD
      Sponsored By: Lexogen
      6
      JUN 07, 2017 | 9:00 AM
      WEBINAR: Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflow
      WEBINAR: Generation of purified human iPSC derived cardiomyocytes...
      DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
      Speaker: Todd Herron, BS, PhD
      Sponsored By: Miltenyi Biotec
      5 1
      MAY 31, 2017 | 6:00 PM
      WEBINAR: Case Studies: Scaling Cell Therapy Manufacturing
      WEBINAR: Case Studies: Scaling Cell Therapy Manufacturing
      DATE: May 31, 2017TIME: 6:00PM PDT, 9:00AM SGT, 12:00PM AEDTGain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through...
      Speaker: Jim Beltzer, MS, PhD
      Sponsored By: TERUMO BCT
      7 1
      MAY 30, 2017 | 9:00 AM
      Protein and Cell Analysis Education Series featuring the Antibody Validation Forum
      Protein and Cell Analysis Education Series featuring the Antibody...
      Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders  Reproducibility and antibody validation standards are two significant challenges f...
      Speaker: Aled Edwards, PhD , Matt Baker , Anita Bandrowski, PhD , Paul Wallace, PhD , John Rogers, PhD , Christoph Hergersberg, PhD
      Presented at: Protein and Cell Analysis Education Series
      15
      MAY 25, 2017 | 6:00 AM
      In-House Technology Development for Next Gen Sequencing, Tissue Handling and beyond, at the BC Cancer Agency
      In-House Technology Development for Next Gen Sequencing, Tissue...
      The Instrumentation Group at the BC Cancer Agency’s Genome Sciences Centre (GSC) is unusual in having an engineering group and extensive prototyping facilities embedded in a biomedical...
      Speaker: Robin Coope
      Presented at: Lab Automation Virtual Event Series 2017
      2
      MAY 24, 2017 | 1:30 PM
      Lab-on-a-printer™ - a multi-material 3D bioprinting platform for building living tissues on demand
      Lab-on-a-printer™ - a multi-material 3D bioprinting platform for...
      Drug development is a costly and time consuming process, poor translation of data from pre-clinical studies to the clinic is a major contributor as late-stage drug failure is very expensive....
      Speaker: Sam Wadsworth, PhD
      Presented at: Lab Automation Virtual Event Series 2017
      2
      MAY 24, 2017 | 12:00 PM
      Hardware Architecture and its use in facilitating collaborative development and dissemination of microfluidic experiments
      Hardware Architecture and its use in facilitating collaborative...
      A hardware architecture was developed specifically to facilitate collaborative development and dissemination of microfluidic experiments. Modular pressure regulators, incubators, and chip-to-...
      Speaker: Carlo Quinonez, PhD
      Presented at: Lab Automation Virtual Event Series 2017
      1
      MAY 24, 2017 | 9:00 AM
      Fully Automated 3D Cell Culture for Human Cells
      Fully Automated 3D Cell Culture for Human Cells
      The consistent and optimized production of living human cells for drug discovery and regenerative medicine faces many challenges including the need for cost effective large scale expansion, i...
      Speaker: Robin Felder, PhD
      Presented at: Lab Automation Virtual Event Series 2017
      2
      MAY 24, 2017 | 7:30 AM
      Case Studies: Scaling Cell Therapy Manufacturing
      Case Studies: Scaling Cell Therapy Manufacturing
      Gain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through the use of a functionally closed, automated, cell culture process....
      Speaker: Jim Beltzer, MS, PhD
      Presented at: Lab Automation Virtual Event Series 2017
      Sponsored By: TERUMO BCT
      1
    JUL 01, 2017 | 8:00 AM
    Journal publishing tools that make YOUR research stand out
    Journal publishing tools that make YOUR research stand out
    There are a number of free and independent scholarly publishing tools available that save journal authors time and ensure research is found, read and tracked. In this talk, we will explore th...
    Speaker: Rebecca Shumbata
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    1
    JUL 01, 2017 | 8:00 AM
    Braingineering's human midbrain organoids as novel model for neurodegenerative diseases
    Braingineering's human midbrain organoids as novel model for neurodegenerative...
    Research on human brain development and neurological diseases is limited by the lack of advanced experimental in vitro models that truly recapitulate the complexity of the human brain. Here, ...
    Speaker: Jens Schwamborn
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    2
    JUL 01, 2017 | 12:00 AM
    Pluripotent stem cells to explore mechanisms and treatments of neuromuscular diseases
    Pluripotent stem cells to explore mechanisms and treatments of...
    Disease-specific human pluripotent stem cells (hPSCs) represent a new chance to unravel cellular and molecular mechanisms of neurological diseases. Along this line, we were among the first to...
    Speaker: Cécile Martinat
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    2
    JUL 01, 2017 | 12:00 AM
    Comparison of the Teratoma assay and in vitro surrogate tests for assessment of pluripotency of human pluripotent stem cells
    Comparison of the Teratoma assay and in vitro surrogate tests...
    For optimal use of human induced pluripotent stem cells (hiPSCs) it is essential to identify lines that are fully reprogrammed and of high quality with proven pluripotency in terms of differe...
    Speaker: Christian Freund
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    2
    JUL 01, 2017 | 12:00 AM
    Physiological Oxygen: Historical and Molecular Perspective for Stem Cell Culture
    Physiological Oxygen: Historical and Molecular Perspective for...
    For many decades, cell lines have been cultured in standard CO2 incubators at “normal” atmospheric oxygen concentrations of about 21%. But oxygen concentrations in the human or an...
    Speaker: Mary Kay Bates, M.S.
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    1
     
     
  • JUL 01, 2017 | 12:00 AM
    New Gibco B-27 Plus Neuronal Culture System-Next Generation Media for Superior Neuronal Survival and Functionality
    New Gibco B-27 Plus Neuronal Culture System-Next Generation Media...
    Since 1993, Gibco™ B-27™ Supplement and Neurobasal™ Medium has been the trusted standard for a variety of neuronal culture applications, with citations in more than 11,000 p...
    Speaker: Navjot Kaur, PhD
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    1
    JUL 01, 2017 | 12:00 AM
    Highly efficient stem cell engineering via Cas9 protein transfection
    Highly efficient stem cell engineering via Cas9 protein transfection
    CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering.  The delivery of Cas9 plasmid DNA or mR...
    Speaker: Xiquan Liang
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    2
    JUL 01, 2017 | 12:00 AM
    New Tools for improved CRISPR gene-editing in stem cells
    New Tools for improved CRISPR gene-editing in stem cells
    The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified stem cell models of human diseases, as well as providing new potential therap...
    Speaker: James Kehler VMD, PhD
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    2
    JUL 01, 2017 | 12:00 AM
    Comprehensive Characterization of Pluripotent Stem Cells
    Comprehensive Characterization of Pluripotent Stem Cells
    As reprogramming methodologies have become more reliable and efficient, corresponding improvements were needed in the characterization workflow as well; specifically in terms of the speed, ef...
    Speaker: Suman Pradhan
    Presented at: 5th annual 24 Hours of Stem Cells™ virtual event
    1
    JUN 28, 2017 | 10:30 AM
    WEBINAR: Variant detection in cell-free DNA from NSCLC using NGS
    WEBINAR: Variant detection in cell-free DNA from NSCLC using NGS
    DATE: June 28, 2017TIME: 10:30am PT, 1:30pm ETThis webinar will describe the implementation of the Oncomine Lung cfDNA Assay from Thermo Fisher Scientific in the Advanced Molecular...
    Speaker: Tracy Stockley PhD, FCCMG, FACMG
    Sponsored By: Thermo Fisher Scientific - Ion Torrent
    6 1
     
     
  • JUN 28, 2017 | 7:00 AM
    Analytical methods to measure empty and full AAV particles
    Analytical methods to measure empty and full AAV particles
    DATE: June 28, 2017TIME: 7:00am PT, 10am ETAs gene therapy approaches usually require large amounts of AAV vectors for clinical use, few manufacturing processes have been repo...
    Speaker: Christine LeBec, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences
    9 2
    JUN 27, 2017 | 6:00 PM
    案例研究:扩大细胞治疗生产规模
    案例研究:扩大细胞治疗生产规模
    不好意思,由于遇到技术困难,此网上讨论会延迟到六月二十八日。若此时间对您不方便,您将可事后从网站上电阅。DATE: June 28, 2017 TIME: 9:00 AM HKTORDATE: June 27, 2017TIME: 6:00 PM PDT 深入了解使用一个功能性密闭状态的自动化细胞培养流...
    Speaker: Jim Beltzer, MS, PhD
    Sponsored By: TERUMO BCT
    4
    JUN 21, 2017 | 7:30 AM
    Five steps for publication-quality fixed-cell imaging the first time
    Five steps for publication-quality fixed-cell imaging the first...
    DATE: June 21, 2017TIME: 7:30am PT, 10:30am ET, 4:30pm CETChoosing the right path to discovery can hasten your success, but the wrong path can lead to missteps that extend the journey a...
    Speaker: Jason Kilgore
    Presented at: Protein and Cell Analysis Education Series
    Sponsored By: Thermo Fisher Scientific, Vert Clinical Leader
    12 1
    JUN 13, 2017 | 9:00 AM
    WEBINAR: High content imaging and analysis for drug discovery: phenotypic assays, instrumentation and software
    WEBINAR: High content imaging and analysis for drug discovery:...
    EVENT DETAILS: DATE: June 13, 2017TIME: 9:00am PT, 12:00pm ETThermo Fisher Scientific is proud to present the SyncD3 webinar series.  As a thought-leader in...
    Speaker: Nicholas Dolman, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    3
    JUN 08, 2017 | 12:00 PM
    Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing the Latest Technologies from Thermo Fisher Scientific
    Improved CRISPR Cas9 Editing of Pluripotent Stem Cells Utilizing...
    The emergence of technology for development of induced pluripotent stem cells (iPSCs) from somatic cells, such as skin and blood cells, has resulted in the ability of researchers to have limi...
    Speaker: Rhonda Newman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    3
     
     
  • JUN 08, 2017 | 10:00 AM
    WEBINAR: Analysis of the transcriptome of carriers of pathological variants in PSEN1, PSEN2 and APP that cause Alzheimer's Disease
    WEBINAR: Analysis of the transcriptome of carriers of pathological...
    DATE: June 8, 2017TIME: 10:00AM PDT, 1:00PM EDT, 7:00PM CESTAlzheimer’s Disease (AD) is the result of complex interactions between risk factors that cause pleiotropic changes in m...
    Speaker: Oscar Harari, PhD
    Sponsored By: Lexogen
    6
    JUN 07, 2017 | 9:00 AM
    WEBINAR: Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflow
    WEBINAR: Generation of purified human iPSC derived cardiomyocytes...
    DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
    Speaker: Todd Herron, BS, PhD
    Sponsored By: Miltenyi Biotec
    5 1
    MAY 31, 2017 | 6:00 PM
    WEBINAR: Case Studies: Scaling Cell Therapy Manufacturing
    WEBINAR: Case Studies: Scaling Cell Therapy Manufacturing
    DATE: May 31, 2017TIME: 6:00PM PDT, 9:00AM SGT, 12:00PM AEDTGain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through...
    Speaker: Jim Beltzer, MS, PhD
    Sponsored By: TERUMO BCT
    7 1
    MAY 30, 2017 | 9:00 AM
    Protein and Cell Analysis Education Series featuring the Antibody Validation Forum
    Protein and Cell Analysis Education Series featuring the Antibody...
    Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders  Reproducibility and antibody validation standards are two significant challenges f...
    Speaker: Aled Edwards, PhD , Matt Baker , Anita Bandrowski, PhD , Paul Wallace, PhD , John Rogers, PhD , Christoph Hergersberg, PhD
    Presented at: Protein and Cell Analysis Education Series
    15
    MAY 25, 2017 | 6:00 AM
    In-House Technology Development for Next Gen Sequencing, Tissue Handling and beyond, at the BC Cancer Agency
    In-House Technology Development for Next Gen Sequencing, Tissue...
    The Instrumentation Group at the BC Cancer Agency’s Genome Sciences Centre (GSC) is unusual in having an engineering group and extensive prototyping facilities embedded in a biomedical...
    Speaker: Robin Coope
    Presented at: Lab Automation Virtual Event Series 2017
    2
     
     
  • MAY 24, 2017 | 1:30 PM
    Lab-on-a-printer™ - a multi-material 3D bioprinting platform for building living tissues on demand
    Lab-on-a-printer™ - a multi-material 3D bioprinting platform for...
    Drug development is a costly and time consuming process, poor translation of data from pre-clinical studies to the clinic is a major contributor as late-stage drug failure is very expensive....
    Speaker: Sam Wadsworth, PhD
    Presented at: Lab Automation Virtual Event Series 2017
    2
    MAY 24, 2017 | 12:00 PM
    Hardware Architecture and its use in facilitating collaborative development and dissemination of microfluidic experiments
    Hardware Architecture and its use in facilitating collaborative...
    A hardware architecture was developed specifically to facilitate collaborative development and dissemination of microfluidic experiments. Modular pressure regulators, incubators, and chip-to-...
    Speaker: Carlo Quinonez, PhD
    Presented at: Lab Automation Virtual Event Series 2017
    1
    MAY 24, 2017 | 9:00 AM
    Fully Automated 3D Cell Culture for Human Cells
    Fully Automated 3D Cell Culture for Human Cells
    The consistent and optimized production of living human cells for drug discovery and regenerative medicine faces many challenges including the need for cost effective large scale expansion, i...
    Speaker: Robin Felder, PhD
    Presented at: Lab Automation Virtual Event Series 2017
    2
    MAY 24, 2017 | 7:30 AM
    Case Studies: Scaling Cell Therapy Manufacturing
    Case Studies: Scaling Cell Therapy Manufacturing
    Gain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through the use of a functionally closed, automated, cell culture process....
    Speaker: Jim Beltzer, MS, PhD
    Presented at: Lab Automation Virtual Event Series 2017
    Sponsored By: TERUMO BCT
    1
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