Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.
DATE: June 8, 2017TIME: 10:00AM PDT, 1:00PM EDT, 7:00PM CESTAlzheimer’s Disease (AD) is the result of complex interactions between risk factors that cause pleiotropic changes in m...
DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
DATE: May 31, 2017TIME: 6:00PM PDT, 9:00AM SGT, 12:00PM AEDTGain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through...
Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders Reproducibility and antibody validation standards are two significant challenges f...
The Instrumentation Group at the BC Cancer Agency’s Genome Sciences Centre (GSC) is unusual in having an engineering group and extensive prototyping facilities embedded in a biomedical...
Drug development is a costly and time consuming process, poor translation of data from pre-clinical studies to the clinic is a major contributor as late-stage drug failure is very expensive....
A hardware architecture was developed specifically to facilitate collaborative development and dissemination of microfluidic experiments. Modular pressure regulators, incubators, and chip-to-...
The consistent and optimized production of living human cells for drug discovery and regenerative medicine faces many challenges including the need for cost effective large scale expansion, i...
Gain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through the use of a functionally closed, automated, cell culture process....
DATE: May 17, 2017TIME: 10:30am PT, 1:30pm ETNeurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and d...
EVENT DETAILS:DATE: May 16, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science our first commit...
Boston Children’s Hospital is developing the infrastructure needed for large-scale psychiatric research and treatment discovery. The Manton Center for Orphan Disease Research and...
Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
Characterizing cellular hierarchy has profound implications in development and diseases. Single-cell technologies have great potential in discovering new cell types and lineage relationships,...
SMRT Sequencing is a DNA sequencing technology characterized by long read lengths and high consensus accuracy, regardless of the sequence complexity or GC content of the DNA sample. These cha...
Glycosylation is an important product quality attribute for biotherapeutic proteins expressed in CHO cells. Glycoform variability can significantly affect the safety and efficacy of therapeut...
Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells. The ability to e...
The isolation of broadly-neutralizing antibodies from HIV-1 infected individuals raises the hope that a vaccine may elicit a similar immune response. These antibodies target the only viral pr...
We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stoc...
Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
Cell free DNA (cfDNA) analysis for the purpose of diagnostics is currently practiced for non-invasive prenatal screening of cell free fetal DNA (cffDNA), and is anticipated to be leveraged fo...
DATE: June 8, 2017TIME: 10:00AM PDT, 1:00PM EDT, 7:00PM CESTAlzheimer’s Disease (AD) is the result of complex interactions between risk factors that cause pleiotropic changes in m...
DATE: June 7, 2017TIME: 9:00AM PDT, 12:00PM EDTPluripotent stem cells (PSCs) offer an unlimited source of human cardiovascular cells for research and the development of cardiac regenera...
DATE: May 31, 2017TIME: 6:00PM PDT, 9:00AM SGT, 12:00PM AEDTGain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through...
Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders Reproducibility and antibody validation standards are two significant challenges f...
The Instrumentation Group at the BC Cancer Agency’s Genome Sciences Centre (GSC) is unusual in having an engineering group and extensive prototyping facilities embedded in a biomedical...
Drug development is a costly and time consuming process, poor translation of data from pre-clinical studies to the clinic is a major contributor as late-stage drug failure is very expensive....
A hardware architecture was developed specifically to facilitate collaborative development and dissemination of microfluidic experiments. Modular pressure regulators, incubators, and chip-to-...
The consistent and optimized production of living human cells for drug discovery and regenerative medicine faces many challenges including the need for cost effective large scale expansion, i...
Gain insights into T-cell expansion, viral vector production and scaling production of mesenchymal stem cells (MSCs) through the use of a functionally closed, automated, cell culture process....
DATE: May 17, 2017TIME: 10:30am PT, 1:30pm ETNeurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and d...
EVENT DETAILS:DATE: May 16, 2017TIME: 9:00am PT, Noon ETThermo Fisher Scientific is proud to present the SyncD3 webinar series. As a thought-leader in science our first commit...
Boston Children’s Hospital is developing the infrastructure needed for large-scale psychiatric research and treatment discovery. The Manton Center for Orphan Disease Research and...
Plasmid purification is the first critical step in ensuring successful transfection and protein expression. As such, it’s important that the right plasmid purification technology and co...
Lentiviral vectors have become the center of attention for its use as gene transfer vectors in gene therapy. Here, we have developed a new lentiviral production system for the clinical grade...
The objective of academic, biotech or pharmaceutical scientists committed to protein purification is to obtain the purest protein needed for their application, regardless of scale. Towards th...
Characterizing cellular hierarchy has profound implications in development and diseases. Single-cell technologies have great potential in discovering new cell types and lineage relationships,...
SMRT Sequencing is a DNA sequencing technology characterized by long read lengths and high consensus accuracy, regardless of the sequence complexity or GC content of the DNA sample. These cha...
Glycosylation is an important product quality attribute for biotherapeutic proteins expressed in CHO cells. Glycoform variability can significantly affect the safety and efficacy of therapeut...
Getting started Equipment Troubleshooting Scale up or scale down your expression runs Tackling difficult-to-express proteins 293 vs. CHO cell expression...
CHO cells are the predominant host for biotherapeutic protein expression with approximately 70% of licensed biologics manufactured in Chinese Hamster Ovary (CHO) cells. The ability to e...
The isolation of broadly-neutralizing antibodies from HIV-1 infected individuals raises the hope that a vaccine may elicit a similar immune response. These antibodies target the only viral pr...
We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stoc...
Development of antibody therapeutics, from early stage research to preclinical and clinical development, requires ever-increasing amounts of reagents. To meet the challenge of furnishing a di...
Cell free DNA (cfDNA) analysis for the purpose of diagnostics is currently practiced for non-invasive prenatal screening of cell free fetal DNA (cffDNA), and is anticipated to be leveraged fo...
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