Single cell sequencing is a next-generation sequencing (NGS) method that examines the genomes or transcriptomes of individual cells, giving a high-resolution view of cell-to-cell variation. Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene expression at single-cell resolution, which greatly revolutionizes transcriptomic studies.
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Long-read DNA sequencing technologies such as the Pacific Biosciences (PacBio) and Oxford Nanopore (ONT) platforms, have demonstrated enhanced detection of genomic variation, including Singl...
Since the introduction of single cell sequencing techniques to the genomics community, interest in its use has grown exponentially and the number of single cell publications has exploded. Th...
Learning Objectives: 1. Learn why the complexity and repetitive structure of the human genome makes it impossible to detect all structural variants using short-read sequencing 2. Learn how h...
Structural variation within plant genomes has fueled domestication and selection of traits critical for modern breeding of crop plants. Nevertheless, dissecting the diversity of structural v...
Single cell RNA sequencing (scRNA-seq) emerged to characterize gene expression differences between individual cells, allowing a higher resolution look at mRNA abundance than bulk RNA- seq. H...