Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.
HEK293 and CHO cells are the primary cell lines used in rapid, scalable suspension culture systems for transient protein expression. This presentation provides a primer on fundamental concept...
The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alt...
Reliable autologous expression of recombinant human proteins - from gene sequence to the expressed and purified protein - in human or CHO cells is essential for many aspects of biomedical res...
DATE: May 9th, 2017TIME: 10:00AM PDT, 1:00PM ETMyeloproliferative neoplasms including myelofibrosis (MF) are characterized by anemia, splenomegaly, bone marrow fibrosis and inflammatory...
DATE: May 9th, 2017TIME: 7:00AM PDT, 10:00AM ET, 2:00PM GMT Early detection and elimination of new chemical entities with cardiac or hERG liability could substantially improve...
DATE: May 2, 2017TIME: 8:00am PT, 11:00am ET, 4:00pm GMT, 5:00pm CETThe natural immune responses that patients develop to their own tumors, as well as therapeutic regimens employin...
Developing therapies for human diseases continues to face obstacles, particularly in translating targets or compounds identified by in vitro screening campaigns to valid targets or efficaciou...
Neurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and drug screening. Human PSCs derived neural stem...
DATE: April 26, 2017TIME: 9:00AM PT, 12:00PM ETBiopsies from non-small cell lung cancer (NSCLC) and colorectal adenocarcinoma generally produce small, heterogeneous samples with li...
DATE: April 20th, 2017TIME: 10:00AM SGT, 11:00AM KST, 12:00PM AEST, 2:00PM NZSTWith increasing breadth and depth of genomics studies across a range of cancers, it is now apparent that t...
Genomics and metagenomics have become ubiquitous research efforts. Here we will show details of the development of physical, computational, and even space-based standards for metagenomi...
With the exception of the Microbiome Quality Control (MBQC), very little has been published on best practices and reference standards for microbiome and metagenomic studies. As evidenced by r...
DATE: April 11, 2017TIME: 8:00 AM PT, 11:00 AM ET Checkpoint blockade therapy for cancer has had tremendous impact on clinical outcomes, yet only a subset of patients respond....
Studying the genetics of rare congenital disorders disrupting cognitive function has led to the identification of multiple disease genes that helped us better understand the mechanisms underl...
Specialized aggregations of extracellular matrix called perineuronal nets (PNNs) appear during juvenile stages of development and surround primarily fast-spiking, parvalbumin (PV)-containing...
Miniaturized microscopes in combination with genetically encoded calcium indicators now allow recordings of activity from hundreds of neurons simultaneously in freely moving animals. We are b...
Gilles de la Tourette syndrome (GTS) is a neurodevelopmental disorder exhibiting both motor and behavioral impairment. Like most neuropsychiatric disorders, its pathophysiology has yet to be...
Dr. Kasthuri is a Neuroscience Researcher at Argonne National Labs and an Assistant Professor (adjunct) in the Dept. of Neurobiology, University of Chicago. He has an MD from Washington...
DATE: March 14, 2017TIME: 8:00AM PST, 11:00AM ESTSurface Plasmon Resonance (SPR) is a widely-used label-free technique to characterize a variety of molecular interactions. SPR is...
Despite vast investment for decades in cancer research and development, cancer is still among the leading causes of death worldwide and its toll is expected to rise by about 70% over the next...
Challenges in achieving comparability, reproducibility and accuracy in biological assays has driven a demand for improved confidence in measurements that support development of regenerative m...
HEK293 and CHO cells are the primary cell lines used in rapid, scalable suspension culture systems for transient protein expression. This presentation provides a primer on fundamental concept...
The ExpiCHO™ transient expression system offers a turnkey solution for generating high-titer recombinant proteins for therapeutic drug development, reagent generation, as well as an alt...
Reliable autologous expression of recombinant human proteins - from gene sequence to the expressed and purified protein - in human or CHO cells is essential for many aspects of biomedical res...
DATE: May 9th, 2017TIME: 10:00AM PDT, 1:00PM ETMyeloproliferative neoplasms including myelofibrosis (MF) are characterized by anemia, splenomegaly, bone marrow fibrosis and inflammatory...
DATE: May 9th, 2017TIME: 7:00AM PDT, 10:00AM ET, 2:00PM GMT Early detection and elimination of new chemical entities with cardiac or hERG liability could substantially improve...
DATE: May 2, 2017TIME: 8:00am PT, 11:00am ET, 4:00pm GMT, 5:00pm CETThe natural immune responses that patients develop to their own tumors, as well as therapeutic regimens employin...
Developing therapies for human diseases continues to face obstacles, particularly in translating targets or compounds identified by in vitro screening campaigns to valid targets or efficaciou...
Neurons derived from human pluripotent stem cells (hPSCs) and primary rodent neurons both are excellent resources for disease modeling and drug screening. Human PSCs derived neural stem...
DATE: April 26, 2017TIME: 9:00AM PT, 12:00PM ETBiopsies from non-small cell lung cancer (NSCLC) and colorectal adenocarcinoma generally produce small, heterogeneous samples with li...
DATE: April 20th, 2017TIME: 10:00AM SGT, 11:00AM KST, 12:00PM AEST, 2:00PM NZSTWith increasing breadth and depth of genomics studies across a range of cancers, it is now apparent that t...
Genomics and metagenomics have become ubiquitous research efforts. Here we will show details of the development of physical, computational, and even space-based standards for metagenomi...
With the exception of the Microbiome Quality Control (MBQC), very little has been published on best practices and reference standards for microbiome and metagenomic studies. As evidenced by r...
DATE: April 11, 2017TIME: 8:00 AM PT, 11:00 AM ET Checkpoint blockade therapy for cancer has had tremendous impact on clinical outcomes, yet only a subset of patients respond....
Studying the genetics of rare congenital disorders disrupting cognitive function has led to the identification of multiple disease genes that helped us better understand the mechanisms underl...
Specialized aggregations of extracellular matrix called perineuronal nets (PNNs) appear during juvenile stages of development and surround primarily fast-spiking, parvalbumin (PV)-containing...
Miniaturized microscopes in combination with genetically encoded calcium indicators now allow recordings of activity from hundreds of neurons simultaneously in freely moving animals. We are b...
Gilles de la Tourette syndrome (GTS) is a neurodevelopmental disorder exhibiting both motor and behavioral impairment. Like most neuropsychiatric disorders, its pathophysiology has yet to be...
Dr. Kasthuri is a Neuroscience Researcher at Argonne National Labs and an Assistant Professor (adjunct) in the Dept. of Neurobiology, University of Chicago. He has an MD from Washington...
DATE: March 14, 2017TIME: 8:00AM PST, 11:00AM ESTSurface Plasmon Resonance (SPR) is a widely-used label-free technique to characterize a variety of molecular interactions. SPR is...
Despite vast investment for decades in cancer research and development, cancer is still among the leading causes of death worldwide and its toll is expected to rise by about 70% over the next...
Challenges in achieving comparability, reproducibility and accuracy in biological assays has driven a demand for improved confidence in measurements that support development of regenerative m...
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