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    Cell Line Development

    Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.

    Webinars

    • FEB 08, 2017 | 1:30 PM
      Safety and pharmacokinetic profiles of different buprenorphine SR doses in a rabbit model
      Safety and pharmacokinetic profiles of different buprenorphine...
      DATE: February 8, 2017TIME: 1:30pm PT, 4:30pm ETIn rabbits, buprenorphine instant release (IR) is commonly used to alleviate pain, especially in surgical models. However, maintenan...
      Speaker: Andrea Zanetti, DVM, PhD, SRS
      Presented at: Laboratory Animal Sciences Virtual Event Series 2017
      3
      FEB 08, 2017 | 10:30 AM
      Biosecurity, Reproductive and Social Management of Cat Research Colonies
      Biosecurity, Reproductive and Social Management of Cat Research...
      DATE: February 8, 2017TIME: 10:30am PT, 1:30pm ETThe cat remains an important comparative species for studying the long-term safety and efficacy of emergent medical modalities, such as...
      Speaker: James Kehler VMD, PhD
      Presented at: Laboratory Animal Sciences Virtual Event Series 2017
      2
      JAN 27, 2017 | 4:00 AM
      WEBINAR: Highly Multiplexed Single Cell Analysis Of Tumor Heterogeneity Through Time and Space by Mass Cytometry
      WEBINAR: Highly Multiplexed Single Cell Analysis Of Tumor Heterogeneity...
      DATE: January 27, 2017TIME: 4:00am PT, 7:00am ET, 1:00pm CETThe study of the tumor ecosystem and its cell-to-cell communications is essential to enable an understanding of tumor biology...
      Speaker: Bernd Bodenmiller, PhD , Randall Wetzel, PhD
      Sponsored By: Cell Signaling Technology, Cell Signaling Technology
      16
      JAN 19, 2017 | 8:00 AM
      Webinar: Detecting meaningful mutations from liquid biopsies by Anchored Multiplex PCR
      Webinar: Detecting meaningful mutations from liquid biopsies by...
      DATE: January 19th, 2017TIME: 8:00AM PT, 11:00AM ETLiquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational st...
      Speaker: Josh Stahl , Jonathan Doose
      Sponsored By: ArcherDX, ArcherDX
      9
      JAN 17, 2017 | 8:00 AM
      Regulation of Bivalent Domains by Histone Modifications
      Regulation of Bivalent Domains by Histone Modifications
      Bivalent domains are thought to be controversial, yet crucial,  regulatory hubs in the differentiation of stem cells. Listen in as  Dr Philipp Voigt of the Wellcome Trust Centre for...
      Speaker: Philipp Voigt, PhD
      Presented at: Abcam virtual exhibition: support and products to advance your research
      4
      JAN 17, 2017 | 8:00 AM
      Single-Cell RNA-Seq and cell heterogeneity in the central nervous system
      Single-Cell RNA-Seq and cell heterogeneity in the central nervous...
      Recent advances in single-cell RNA sequencing are producing exciting new insights into cell diversity within different tissues and cancers. Listen in to hear  Dr Gonçalo Castelo-B...
      Speaker: Gonçalo Castelo-Branco, PhD , Amit Zeisel, PhD
      Presented at: Abcam virtual exhibition: support and products to advance your research
      5
      JAN 17, 2017 | 8:00 AM
      An Introduction to In-Cell ELISA
      An Introduction to In-Cell ELISA
      In-Cell ELISA, is a well-accepted assay approach to perform quantitative immunocytochemistry of cultured cells using either a colormetric or fluorometric readout.  Join our In-Cell ELISA...
      Speaker: John Constable, PhD
      Presented at: Abcam virtual exhibition: support and products to advance your research
      4
      JAN 17, 2017 | 8:00 AM
      Benefits and limitations of primary and secondary antibody conjugates
      Benefits and limitations of primary and secondary antibody conjugates
      Join Dr Javier Conde Vancells as he reviews the use of primary and secondary antibody conjugates across multiple lab applications, including a review of direct vs. indirect methods in cellula...
      Speaker: Javier Vancells, PhD
      Presented at: Abcam virtual exhibition: support and products to advance your research
      4
      DEC 14, 2016 | 7:30 AM
      Advances in modeling human diseases using induced pluripotent stem cell-derived cellular models
      Advances in modeling human diseases using induced pluripotent...
      DATE: December 14, 2016 TIME: 7:30am PT, 10:30am ET A major hurdle in developing relevant disease models for drug discovery is access to the healthy and diseased tissue of interest; induc...
      Speaker: T.K. Feaster, PhD , Kile Mangan, PhD
      Sponsored By: Cellular Dynamics, Cellular Dynamics
      7 1
      DEC 07, 2016 | 9:00 AM
      The Interplay of Kinase Broad Profiling and Phenotypic Screening
      The Interplay of Kinase Broad Profiling and Phenotypic Screening
      DATE: December 7, 2016 TIME: 9:00 AM PT, 12:00 PM ET Kinases play important roles in many biological signaling pathways. Kinase inhibitors are therefore potentially found as actives in ty...
      Speaker: Edgar Jacoby, Ph.D.
      Sponsored By: DiscoverX, DiscoverX
      4
      DEC 06, 2016 | 11:00 AM
      Expansion of Human Stem Cells in BioBLU Single-Use Vessels from Eppendorf
      Expansion of Human Stem Cells in BioBLU Single-Use Vessels from...
      DATE: December 6, 2016 TIME: 11:00am PT, 2:00pm ET Experimentation involving human stem cells (HSCs) is one of the fastest growing fields in research and development due, in large part, t...
      Speaker: Stacey Willard, PhD
      Sponsored By: Eppendorf, Eppendorf
      5 1
      DEC 01, 2016 | 8:00 AM
      In Vivo Imaging Toolkit: Advancing Preclinical and Translational Science in Molecular and Orthopedic Research at UCLA
      In Vivo Imaging Toolkit: Advancing Preclinical and Translational...
      DATE: December 1, 2016TIME:  8:00 AM PT, 11:00 AM ETPreclinical imaging enables the measurement and assessment of biological processes in vivo utilizing technologies such as biolum...
      Speaker: Nick Bernthal, MD , Jason Lee, Ph.D
      Sponsored By: Revvity
      3
      NOV 23, 2016 | 8:00 AM
      Studying Kinetics of Chromatin Assembly with SWATH-MS
      Studying Kinetics of Chromatin Assembly with SWATH-MS
      DATE: November 23rd, 2016TIME: 8:00AM PT, 11:00AM ETThe structure of chromatin is critical for many aspects of cellular physiology and is considered to be the primary medium to store ep...
      Speaker: Moritz Voelker-Albert , Andreas Schmidt
      Sponsored By: SCIEX OMICS, SCIEX OMICS
      24
      NOV 17, 2016 | 3:00 PM
      Molecular elucidation and engineering of stem cell fate decisions
      Molecular elucidation and engineering of stem cell fate decisions
      Stem cells play critical roles in the development of organisms, as well as in the maintenance and repair of organs and tissues throughout adulthood.  Advancing our understanding of mecha...
      Speaker: David Schaffer, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      4
      NOV 17, 2016 | 1:00 PM
      Initiatives to advance stem cell science and medicine at California's $3 billion stem cell agency
      Initiatives to advance stem cell science and medicine at California's...
      The mission of the California Institute for Regenerative Medicine (CIRM) is to accelerate stem cell treatments to patients with unmet medical needs.  With $3 billion in funding and 300 a...
      Speaker: Stephen Lin, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      4
      NOV 17, 2016 | 9:00 AM
      From bench to BLA - a review of common regulatory questions
      From bench to BLA - a review of common regulatory questions
      This panel will discuss quality requirements of ancillary materials used in cell therapy manufacturing, relevant standards and how requirements vary by phase.  Participants will learn wh...
      Speaker: Joyce Frey-Vasconcells, PhD , Michael Mendicino, PhD , Kelli Tanzella, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      3
      NOV 17, 2016 | 8:30 AM
      Simultaneous, single-cell visualization of RNA and Protein
      Simultaneous, single-cell visualization of RNA and Protein
      DATE: November 17, 2016 TIME: 8:30am PT, 11:30am ET The complex interactions between transcription, translation, and post-translational modifications are hidden from view in typ...
      Speaker: Sara Becker-Catania, PhD
      Sponsored By: eBioscience Business Unit, Thermo Fisher Scientific, eBioscience Business Unit, Thermo Fisher Scientific
      4
      NOV 17, 2016 | 5:00 AM
      Partnering to advance regenerative medicine and cellular therapies
      Partnering to advance regenerative medicine and cellular therapies
      The Alliance for Regenerative Medicine (ARM) is the preeminent global advocate for regenerative and advanced therapies. ARM fosters research, development, investment and commercialization of ...
      Speaker: Michael Werner , Morrie Ruffin, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      4
      NOV 17, 2016 | 3:00 AM
      Genetic variability of ps-iPSC and related blood and fibroblasts - somatic mutations
      Genetic variability of ps-iPSC and related blood and fibroblasts...
      The generation of induced pluripotent stem cells (iPSCs) from adult easily accessible somatic tissues was introduced ten years ago. This technology has revolutionized our opportunities to stu...
      Speaker: KEYNOTE: Silke Rickert-Sperling, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      8 1
      NOV 16, 2016 | 6:00 PM
      Optimization of your PSCs workflow
      Optimization of your PSCs workflow
      Background: Applications and general workflow of PSCs Reprogramming with high efficiency and safety (Summary of current reprogramming methods, Introduction of Cytotune-iPS 2.0 Sendai Rep...
      Speaker: Jing He, PhD 何静 博士
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      3
      NOV 16, 2016 | 4:00 PM
      Evolving your media system for modern stem cell applications
      Evolving your media system for modern stem cell applications
      Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeut...
      Speaker: Rhonda Newman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      4
      NOV 15, 2016 | 9:00 AM
      Antigen-specific, poly-functional CD4+ T cells are required for vaccine-mediated protection in tularemia
      Antigen-specific, poly-functional CD4+ T cells are required for...
      DATE: November 15, 2016 TIME: 9:00 AM PT, 12:00 PM ET The virulent intracellular pathogen Francisella tularensis subsp. tularensis (Ftt) causes an acute, lethal diseas...
      Speaker: Lydia Roberts, PhD
      Sponsored By: Miltenyi Biotec, Miltenyi Biotec
      3
      NOV 10, 2016 | 7:30 AM
      Does your protein analysis toolbox need a refresh?
      Does your protein analysis toolbox need a refresh?
      DATE:  November 10, 2016 TIME:  7:30am PT, 10:30am ET  Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each migh...
      Speaker: Rich Jones, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      4
      NOV 09, 2016 | 9:00 AM
      A duplexed assay to measure GPCR-mediated calcium signaling and β-Arrestin recruitment from single well using FDSS/µCell functional screening system
      A duplexed assay to measure GPCR-mediated calcium signaling and...
      DATE: November 9th, 2016 TIME: 9:00 AM PT, 12:00 PM ET The screening and functional characterization of novel ligands for G-Protein Coupled Receptors (GPCR) remains a key focus in drug di...
      Speaker: Thierry Calmels, PhD
      Sponsored By: DiscoverX, DiscoverX
      3
    FEB 08, 2017 | 1:30 PM
    Safety and pharmacokinetic profiles of different buprenorphine SR doses in a rabbit model
    Safety and pharmacokinetic profiles of different buprenorphine...
    DATE: February 8, 2017TIME: 1:30pm PT, 4:30pm ETIn rabbits, buprenorphine instant release (IR) is commonly used to alleviate pain, especially in surgical models. However, maintenan...
    Speaker: Andrea Zanetti, DVM, PhD, SRS
    Presented at: Laboratory Animal Sciences Virtual Event Series 2017
    3
    FEB 08, 2017 | 10:30 AM
    Biosecurity, Reproductive and Social Management of Cat Research Colonies
    Biosecurity, Reproductive and Social Management of Cat Research...
    DATE: February 8, 2017TIME: 10:30am PT, 1:30pm ETThe cat remains an important comparative species for studying the long-term safety and efficacy of emergent medical modalities, such as...
    Speaker: James Kehler VMD, PhD
    Presented at: Laboratory Animal Sciences Virtual Event Series 2017
    2
    JAN 27, 2017 | 4:00 AM
    WEBINAR: Highly Multiplexed Single Cell Analysis Of Tumor Heterogeneity Through Time and Space by Mass Cytometry
    WEBINAR: Highly Multiplexed Single Cell Analysis Of Tumor Heterogeneity...
    DATE: January 27, 2017TIME: 4:00am PT, 7:00am ET, 1:00pm CETThe study of the tumor ecosystem and its cell-to-cell communications is essential to enable an understanding of tumor biology...
    Speaker: Bernd Bodenmiller, PhD , Randall Wetzel, PhD
    Sponsored By: Cell Signaling Technology, Cell Signaling Technology
    16
    JAN 19, 2017 | 8:00 AM
    Webinar: Detecting meaningful mutations from liquid biopsies by Anchored Multiplex PCR
    Webinar: Detecting meaningful mutations from liquid biopsies by...
    DATE: January 19th, 2017TIME: 8:00AM PT, 11:00AM ETLiquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational st...
    Speaker: Josh Stahl , Jonathan Doose
    Sponsored By: ArcherDX, ArcherDX
    9
    JAN 17, 2017 | 8:00 AM
    Regulation of Bivalent Domains by Histone Modifications
    Regulation of Bivalent Domains by Histone Modifications
    Bivalent domains are thought to be controversial, yet crucial,  regulatory hubs in the differentiation of stem cells. Listen in as  Dr Philipp Voigt of the Wellcome Trust Centre for...
    Speaker: Philipp Voigt, PhD
    Presented at: Abcam virtual exhibition: support and products to advance your research
    4
     
     
  • JAN 17, 2017 | 8:00 AM
    Single-Cell RNA-Seq and cell heterogeneity in the central nervous system
    Single-Cell RNA-Seq and cell heterogeneity in the central nervous...
    Recent advances in single-cell RNA sequencing are producing exciting new insights into cell diversity within different tissues and cancers. Listen in to hear  Dr Gonçalo Castelo-B...
    Speaker: Gonçalo Castelo-Branco, PhD , Amit Zeisel, PhD
    Presented at: Abcam virtual exhibition: support and products to advance your research
    5
    JAN 17, 2017 | 8:00 AM
    An Introduction to In-Cell ELISA
    An Introduction to In-Cell ELISA
    In-Cell ELISA, is a well-accepted assay approach to perform quantitative immunocytochemistry of cultured cells using either a colormetric or fluorometric readout.  Join our In-Cell ELISA...
    Speaker: John Constable, PhD
    Presented at: Abcam virtual exhibition: support and products to advance your research
    4
    JAN 17, 2017 | 8:00 AM
    Benefits and limitations of primary and secondary antibody conjugates
    Benefits and limitations of primary and secondary antibody conjugates
    Join Dr Javier Conde Vancells as he reviews the use of primary and secondary antibody conjugates across multiple lab applications, including a review of direct vs. indirect methods in cellula...
    Speaker: Javier Vancells, PhD
    Presented at: Abcam virtual exhibition: support and products to advance your research
    4
    DEC 14, 2016 | 7:30 AM
    Advances in modeling human diseases using induced pluripotent stem cell-derived cellular models
    Advances in modeling human diseases using induced pluripotent...
    DATE: December 14, 2016 TIME: 7:30am PT, 10:30am ET A major hurdle in developing relevant disease models for drug discovery is access to the healthy and diseased tissue of interest; induc...
    Speaker: T.K. Feaster, PhD , Kile Mangan, PhD
    Sponsored By: Cellular Dynamics, Cellular Dynamics
    7 1
    DEC 07, 2016 | 9:00 AM
    The Interplay of Kinase Broad Profiling and Phenotypic Screening
    The Interplay of Kinase Broad Profiling and Phenotypic Screening
    DATE: December 7, 2016 TIME: 9:00 AM PT, 12:00 PM ET Kinases play important roles in many biological signaling pathways. Kinase inhibitors are therefore potentially found as actives in ty...
    Speaker: Edgar Jacoby, Ph.D.
    Sponsored By: DiscoverX, DiscoverX
    4
     
     
  • DEC 06, 2016 | 11:00 AM
    Expansion of Human Stem Cells in BioBLU Single-Use Vessels from Eppendorf
    Expansion of Human Stem Cells in BioBLU Single-Use Vessels from...
    DATE: December 6, 2016 TIME: 11:00am PT, 2:00pm ET Experimentation involving human stem cells (HSCs) is one of the fastest growing fields in research and development due, in large part, t...
    Speaker: Stacey Willard, PhD
    Sponsored By: Eppendorf, Eppendorf
    5 1
    DEC 01, 2016 | 8:00 AM
    In Vivo Imaging Toolkit: Advancing Preclinical and Translational Science in Molecular and Orthopedic Research at UCLA
    In Vivo Imaging Toolkit: Advancing Preclinical and Translational...
    DATE: December 1, 2016TIME:  8:00 AM PT, 11:00 AM ETPreclinical imaging enables the measurement and assessment of biological processes in vivo utilizing technologies such as biolum...
    Speaker: Nick Bernthal, MD , Jason Lee, Ph.D
    Sponsored By: Revvity
    3
    NOV 23, 2016 | 8:00 AM
    Studying Kinetics of Chromatin Assembly with SWATH-MS
    Studying Kinetics of Chromatin Assembly with SWATH-MS
    DATE: November 23rd, 2016TIME: 8:00AM PT, 11:00AM ETThe structure of chromatin is critical for many aspects of cellular physiology and is considered to be the primary medium to store ep...
    Speaker: Moritz Voelker-Albert , Andreas Schmidt
    Sponsored By: SCIEX OMICS, SCIEX OMICS
    24
    NOV 17, 2016 | 3:00 PM
    Molecular elucidation and engineering of stem cell fate decisions
    Molecular elucidation and engineering of stem cell fate decisions
    Stem cells play critical roles in the development of organisms, as well as in the maintenance and repair of organs and tissues throughout adulthood.  Advancing our understanding of mecha...
    Speaker: David Schaffer, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    4
    NOV 17, 2016 | 1:00 PM
    Initiatives to advance stem cell science and medicine at California's $3 billion stem cell agency
    Initiatives to advance stem cell science and medicine at California's...
    The mission of the California Institute for Regenerative Medicine (CIRM) is to accelerate stem cell treatments to patients with unmet medical needs.  With $3 billion in funding and 300 a...
    Speaker: Stephen Lin, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    4
     
     
  • NOV 17, 2016 | 9:00 AM
    From bench to BLA - a review of common regulatory questions
    From bench to BLA - a review of common regulatory questions
    This panel will discuss quality requirements of ancillary materials used in cell therapy manufacturing, relevant standards and how requirements vary by phase.  Participants will learn wh...
    Speaker: Joyce Frey-Vasconcells, PhD , Michael Mendicino, PhD , Kelli Tanzella, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    3
    NOV 17, 2016 | 8:30 AM
    Simultaneous, single-cell visualization of RNA and Protein
    Simultaneous, single-cell visualization of RNA and Protein
    DATE: November 17, 2016 TIME: 8:30am PT, 11:30am ET The complex interactions between transcription, translation, and post-translational modifications are hidden from view in typ...
    Speaker: Sara Becker-Catania, PhD
    Sponsored By: eBioscience Business Unit, Thermo Fisher Scientific, eBioscience Business Unit, Thermo Fisher Scientific
    4
    NOV 17, 2016 | 5:00 AM
    Partnering to advance regenerative medicine and cellular therapies
    Partnering to advance regenerative medicine and cellular therapies
    The Alliance for Regenerative Medicine (ARM) is the preeminent global advocate for regenerative and advanced therapies. ARM fosters research, development, investment and commercialization of ...
    Speaker: Michael Werner , Morrie Ruffin, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    4
    NOV 17, 2016 | 3:00 AM
    Genetic variability of ps-iPSC and related blood and fibroblasts - somatic mutations
    Genetic variability of ps-iPSC and related blood and fibroblasts...
    The generation of induced pluripotent stem cells (iPSCs) from adult easily accessible somatic tissues was introduced ten years ago. This technology has revolutionized our opportunities to stu...
    Speaker: KEYNOTE: Silke Rickert-Sperling, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    8 1
    NOV 16, 2016 | 6:00 PM
    Optimization of your PSCs workflow
    Optimization of your PSCs workflow
    Background: Applications and general workflow of PSCs Reprogramming with high efficiency and safety (Summary of current reprogramming methods, Introduction of Cytotune-iPS 2.0 Sendai Rep...
    Speaker: Jing He, PhD 何静 博士
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    3
     
     
  • NOV 16, 2016 | 4:00 PM
    Evolving your media system for modern stem cell applications
    Evolving your media system for modern stem cell applications
    Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeut...
    Speaker: Rhonda Newman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    4
    NOV 15, 2016 | 9:00 AM
    Antigen-specific, poly-functional CD4+ T cells are required for vaccine-mediated protection in tularemia
    Antigen-specific, poly-functional CD4+ T cells are required for...
    DATE: November 15, 2016 TIME: 9:00 AM PT, 12:00 PM ET The virulent intracellular pathogen Francisella tularensis subsp. tularensis (Ftt) causes an acute, lethal diseas...
    Speaker: Lydia Roberts, PhD
    Sponsored By: Miltenyi Biotec, Miltenyi Biotec
    3
    NOV 10, 2016 | 7:30 AM
    Does your protein analysis toolbox need a refresh?
    Does your protein analysis toolbox need a refresh?
    DATE:  November 10, 2016 TIME:  7:30am PT, 10:30am ET  Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each migh...
    Speaker: Rich Jones, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    4
    NOV 09, 2016 | 9:00 AM
    A duplexed assay to measure GPCR-mediated calcium signaling and β-Arrestin recruitment from single well using FDSS/µCell functional screening system
    A duplexed assay to measure GPCR-mediated calcium signaling and...
    DATE: November 9th, 2016 TIME: 9:00 AM PT, 12:00 PM ET The screening and functional characterization of novel ligands for G-Protein Coupled Receptors (GPCR) remains a key focus in drug di...
    Speaker: Thierry Calmels, PhD
    Sponsored By: DiscoverX, DiscoverX
    3
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