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    Cell Line Development

    Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.

    Webinars

    • NOV 08, 2016 | 6:00 AM
      New Approaches to Antibody Validation Using Immunoprecipitation and Mass Spectrometry
      New Approaches to Antibody Validation Using Immunoprecipitation...
      Antibodies are used in a broad range of research and diagnostic applications for the enrichment, detection, and quantitation of proteins and their modifications.  Hundreds of thousands o...
      Speaker: John Rogers, PhD
      Presented at: Protein and Cell Analysis Education Series
      Sponsored By: Thermo Fisher Scientific
      9 2
      NOV 03, 2016 | 10:30 AM
      Rapid, Low-cost Tools for Global Health:  Using Cell-free Synthetic Biology for Zika Virus Detection and the Portable Manufacture of Therapeutics
      Rapid, Low-cost Tools for Global Health: Using Cell-free Synthetic...
      The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions.  In early 2016 we developed a pipe...
      Speaker: Keith Pardee, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      3
      NOV 02, 2016 | 1:30 PM
      A Platform to Digitize Biology: A Potential Pathway to Exponential Medicine
      A Platform to Digitize Biology: A Potential Pathway to Exponential...
      Today there are about 7B mobile phone worldwide and about 50,000 mobile health applications actively changing the landscape of how healthcare will be delivered in the next years. While numero...
      Speaker: John McDevitt, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      4
      NOV 02, 2016 | 10:30 AM
      Tough Targets. Simple Genotyping. Using HRM as a direct approach in research applications.
      Tough Targets. Simple Genotyping. Using HRM as a direct approach...
      Are there DNA targets in your lab that are difficult to genotype using standard PCR or sequencing?Do you ever need to genotype a single sample, and you don’t want to wait to batch sam...
      Speaker: Brian McNally, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      2
      NOV 01, 2016 | 8:00 AM
      Development of selection strategies for Homologous Recombination Deficient (HRD) patients utilizing a Liquid Biopsy
      Development of selection strategies for Homologous Recombination...
      DATE: November 1, 2016 TIME: 8:00AM PT, 11:00AM ET Metastatic cancer is an evolving, heterogeneous disease that becomes more complex over time through the selection of sub-clonal tumor po...
      Speaker: Mark Landers
      Sponsored By: Epic Sciences, Epic Sciences , Epic Sciences
      4
      OCT 27, 2016 | 9:00 AM
      Achieving Reproducibility: Don't Let Antibodies be a Variable in Your Experiment
      Achieving Reproducibility: Don't Let Antibodies be a Variable...
      DATE:  October 27, 2016 TIME:  9:00am PT, 12:00pm ET The importance of antibodies as tools in scientific research studies cannot be understated, yet these reagents have increasi...
      Speaker: Anthony Couvillon, PhD
      Sponsored By: Cell Signaling Technology, Cell Signaling Technology
      10
      OCT 11, 2016 | 7:00 AM
      Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates
      Two Dimensional Liquid Chromatography Applied to the Characterization...
      DATE:  October 11, 2016 TIME:  7:00am PT, 10:00am ET Monoclonal antibodies (mAbs) have emerged as important therapeutics for the treatment of life-threatening diseases. The succ...
      Speaker: Koen Sandra, PhD
      Sponsored By: Agilent Genomics, Agilent Genomics
      5
      OCT 11, 2016 | 7:00 AM
      Cellular Applications for Image-based Cytometry on A Multimode Plate Reader
      Cellular Applications for Image-based Cytometry on A Multimode...
      Date: October 11th, 2016Time: 10:00AM EST, 4:00PM CEST, 3:00PM BST, 7:00AM PSTPlate readers first became available about 30 years ago and have become the workhorses of researc...
      Speaker: Frauke Haenel, PhD , Maria Kuzikov, MSc
      Sponsored By: Revvity
      8 1
      OCT 06, 2016 | 10:30 AM
      Precision Genomics and Precision Medicine for Immuno-Oncology
      Precision Genomics and Precision Medicine for Immuno-Oncology
      Tumors often evade immune destruction by adapting and circumventing immune checkpoints to create resistance.  This adaptive immunity can be reversed to stimulate the endogenous immune re...
      Speaker: Erin Newburn, MS, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      0
      OCT 06, 2016 | 9:00 AM
      Keynote Speaker - PD-1 cancer immunotherapy
      Keynote Speaker - PD-1 cancer immunotherapy
      The immune system is capable of recognizing tumors and eliminates many early malignant cells. However, tumors evolve to evade immune attack, and the tumor microenvironment is immunosuppressiv...
      Speaker: Gordon Freeman, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      OCT 05, 2016 | 10:30 AM
      Immunotherapy of Cancer Using Genetically-Enhanced T Cells
      Immunotherapy of Cancer Using Genetically-Enhanced T Cells
      The immune system possesses significant cytotoxic potential. Stimulating natural immunity through vaccination has shown promising effects in some cancers; however, a number of barriers limit...
      Speaker: Michael Milone, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      OCT 05, 2016 | 10:30 AM
      Unraveling the Complexities of Immuno-Oncology with 3D Biologyâ„¢ Technology
      Unraveling the Complexities of Immuno-Oncology with 3D Biologyâ„¢...
      The ability to measure changes to DNA, RNA, and protein across cell types is crucial to understanding the immune response to the environment or therapeutic treatment. NanoString Technologies...
      Speaker: Joseph Beechem, PhD , Alessandra Cesano, MD, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      OCT 05, 2016 | 6:00 AM
      Melanoma - View from the Frontlines of Immunotherapy and Precision Medicine
      Melanoma - View from the Frontlines of Immunotherapy and Precision...
      Melanoma arises in the pigment producing cells (melanocytes) and is the deadliest of the skin cancers. It accounts for nearly 200,000 new cases of cancer each year worldwide and in the U.S. o...
      Speaker: Louise Perkins, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      SEP 29, 2016 | 8:00 AM
      Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids
      Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles...
      PRESENTED BY:DATE:  September 29, 2016TIME:  8:00AM PT, 11AM ETInterest in 3D cell culture has grown exponentially in recent years, partially due to 3D cultures hel...
      Speaker: Dr. Glauco Souza, BS, MS, PhD
      Sponsored By: Cytiva
      5
      SEP 28, 2016 | 9:00 AM
      Signatures of protective NK cell responses
      Signatures of protective NK cell responses
      DATE:  September 28, 2016TIME:   9:00am Pacific time, 12:00pm Eastern timeNK cells play critical roles in immune defense and reproduction but remain the most poorly under...
      Speaker: Catherine Blish, MD, PhD , Michelle Poulin, PhD
      Sponsored By: Standard BioTools, Standard BioTools
      9 1
      SEP 22, 2016 | 4:00 AM
      Cross-validation of Antibodies Using DigiWest
      Cross-validation of Antibodies Using DigiWest
      DATE: September 22, 2016 TIME:  4:00am PT, 7:00am ET, 11:00am GMT Here, we present a novel approach that combines the principles of the western blot (protein separation by SDS-PAGE; ...
      Speaker: Anthony Couvillon, PhD , Markus Templin, PhD
      Sponsored By: Cell Signaling Technology, Cell Signaling Technology
      3 2
      SEP 21, 2016 | 7:30 AM
      Using Data to Drive Automated Screening and Effective Reporting
      Using Data to Drive Automated Screening and Effective Reporting
      The most important job of automated biology is to produce results that moves your research forward. If automation only executes a program of pre-determined set of steps, the researcher is lef...
      Speaker: Tim Sherrill , Mike Kowalski, Ph.D.
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      6
      SEP 20, 2016 | 7:00 AM
      Advanced Analysis of Complex Cell Models with the New Operetta CLS HCA System
      Advanced Analysis of Complex Cell Models with the New Operetta...
      DATE: September 20th, 2016TIME:  7:00AM PST, 10:00AM ETThere is a growing trend towards developing in vitro cell models that recapitulate the in vivo environment in basic research,...
      Speaker: Jacob Tesdorpf, PhD , Joe Trask
      Sponsored By: Revvity
      25
      SEP 07, 2016 | 10:30 AM
      Exploring the Great Indoors: Relevance of the Built Environment to Human Health
      Exploring the Great Indoors: Relevance of the Built Environment...
      The human body is populated with trillions of microorganisms, collectively termed the human microbiome, that play vital roles in health including nutrition and metabolism, immune development,...
      Speaker: Roxana Hickey, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2016
      2
      SEP 07, 2016 | 6:00 AM
      Invisible Influence: How the indoor microbiome influences health
      Invisible Influence: How the indoor microbiome influences health
      The human race, like all macrobiological life, evolved in a sea of microbes. There was no way to keep the bacterial and archaeal hoards at bay, so instead life evolved mechanisms to live with...
      Speaker: Jack Gilbert, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2016
      2
      AUG 30, 2016 | 8:00 AM
      iPSC Generation and Validation: Ensuring the best materials for downstream applications  iPSC Generation and Validation: Ensuring the best materials for downstream applications
      iPSC Generation and Validation: Ensuring the best materials for...
      The reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSC) has great potential of applications, not only in basic research, but also in drug screening and cell therapy. Wit...
      Speaker: Rene Quintanilla Jr.
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      0
      AUG 30, 2016 | 8:00 AM
      Improved differentiation of human pluripotent stem cell-derived neurons through reduction of progenitor proliferation: impact on downstream applications
      Improved differentiation of human pluripotent stem cell-derived...
      Neurons derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are excellent resources for disease modeling and d...
      Speaker: David Kuninger, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      1
      AUG 30, 2016 | 8:00 AM
      Stem Cells for Regeneration and Rescue
      Stem Cells for Regeneration and Rescue
      Most discussions about pluripotent stem cells center around their promise for regenerative medicine.  The most remarkable quality of these cells is their ability to turn into all of the ...
      Speaker: Jeanne Loring
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      3
      AUG 30, 2016 | 8:00 AM
      Tissue engineering with human pluripotent stem cells
      Tissue engineering with human pluripotent stem cells
      One of the ultimate goals in Regenerative Medicine is the generation of pluripotent stem cells (PSCs) directly from somatic cells obtained from patients. Although major findings in the defini...
      Speaker: Nuria Montserrat, PhD
      Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
      1
    NOV 08, 2016 | 6:00 AM
    New Approaches to Antibody Validation Using Immunoprecipitation and Mass Spectrometry
    New Approaches to Antibody Validation Using Immunoprecipitation...
    Antibodies are used in a broad range of research and diagnostic applications for the enrichment, detection, and quantitation of proteins and their modifications.  Hundreds of thousands o...
    Speaker: John Rogers, PhD
    Presented at: Protein and Cell Analysis Education Series
    Sponsored By: Thermo Fisher Scientific
    9 2
    NOV 03, 2016 | 10:30 AM
    Rapid, Low-cost Tools for Global Health:  Using Cell-free Synthetic Biology for Zika Virus Detection and the Portable Manufacture of Therapeutics
    Rapid, Low-cost Tools for Global Health: Using Cell-free Synthetic...
    The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions.  In early 2016 we developed a pipe...
    Speaker: Keith Pardee, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    3
    NOV 02, 2016 | 1:30 PM
    A Platform to Digitize Biology: A Potential Pathway to Exponential Medicine
    A Platform to Digitize Biology: A Potential Pathway to Exponential...
    Today there are about 7B mobile phone worldwide and about 50,000 mobile health applications actively changing the landscape of how healthcare will be delivered in the next years. While numero...
    Speaker: John McDevitt, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    4
    NOV 02, 2016 | 10:30 AM
    Tough Targets. Simple Genotyping. Using HRM as a direct approach in research applications.
    Tough Targets. Simple Genotyping. Using HRM as a direct approach...
    Are there DNA targets in your lab that are difficult to genotype using standard PCR or sequencing?Do you ever need to genotype a single sample, and you don’t want to wait to batch sam...
    Speaker: Brian McNally, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    2
    NOV 01, 2016 | 8:00 AM
    Development of selection strategies for Homologous Recombination Deficient (HRD) patients utilizing a Liquid Biopsy
    Development of selection strategies for Homologous Recombination...
    DATE: November 1, 2016 TIME: 8:00AM PT, 11:00AM ET Metastatic cancer is an evolving, heterogeneous disease that becomes more complex over time through the selection of sub-clonal tumor po...
    Speaker: Mark Landers
    Sponsored By: Epic Sciences, Epic Sciences , Epic Sciences
    4
     
     
  • OCT 27, 2016 | 9:00 AM
    Achieving Reproducibility: Don't Let Antibodies be a Variable in Your Experiment
    Achieving Reproducibility: Don't Let Antibodies be a Variable...
    DATE:  October 27, 2016 TIME:  9:00am PT, 12:00pm ET The importance of antibodies as tools in scientific research studies cannot be understated, yet these reagents have increasi...
    Speaker: Anthony Couvillon, PhD
    Sponsored By: Cell Signaling Technology, Cell Signaling Technology
    10
    OCT 11, 2016 | 7:00 AM
    Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates
    Two Dimensional Liquid Chromatography Applied to the Characterization...
    DATE:  October 11, 2016 TIME:  7:00am PT, 10:00am ET Monoclonal antibodies (mAbs) have emerged as important therapeutics for the treatment of life-threatening diseases. The succ...
    Speaker: Koen Sandra, PhD
    Sponsored By: Agilent Genomics, Agilent Genomics
    5
    OCT 11, 2016 | 7:00 AM
    Cellular Applications for Image-based Cytometry on A Multimode Plate Reader
    Cellular Applications for Image-based Cytometry on A Multimode...
    Date: October 11th, 2016Time: 10:00AM EST, 4:00PM CEST, 3:00PM BST, 7:00AM PSTPlate readers first became available about 30 years ago and have become the workhorses of researc...
    Speaker: Frauke Haenel, PhD , Maria Kuzikov, MSc
    Sponsored By: Revvity
    8 1
    OCT 06, 2016 | 10:30 AM
    Precision Genomics and Precision Medicine for Immuno-Oncology
    Precision Genomics and Precision Medicine for Immuno-Oncology
    Tumors often evade immune destruction by adapting and circumventing immune checkpoints to create resistance.  This adaptive immunity can be reversed to stimulate the endogenous immune re...
    Speaker: Erin Newburn, MS, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    0
    OCT 06, 2016 | 9:00 AM
    Keynote Speaker - PD-1 cancer immunotherapy
    Keynote Speaker - PD-1 cancer immunotherapy
    The immune system is capable of recognizing tumors and eliminates many early malignant cells. However, tumors evolve to evade immune attack, and the tumor microenvironment is immunosuppressiv...
    Speaker: Gordon Freeman, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
     
     
  • OCT 05, 2016 | 10:30 AM
    Immunotherapy of Cancer Using Genetically-Enhanced T Cells
    Immunotherapy of Cancer Using Genetically-Enhanced T Cells
    The immune system possesses significant cytotoxic potential. Stimulating natural immunity through vaccination has shown promising effects in some cancers; however, a number of barriers limit...
    Speaker: Michael Milone, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
    OCT 05, 2016 | 10:30 AM
    Unraveling the Complexities of Immuno-Oncology with 3D Biologyâ„¢ Technology
    Unraveling the Complexities of Immuno-Oncology with 3D Biologyâ„¢...
    The ability to measure changes to DNA, RNA, and protein across cell types is crucial to understanding the immune response to the environment or therapeutic treatment. NanoString Technologies...
    Speaker: Joseph Beechem, PhD , Alessandra Cesano, MD, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
    OCT 05, 2016 | 6:00 AM
    Melanoma - View from the Frontlines of Immunotherapy and Precision Medicine
    Melanoma - View from the Frontlines of Immunotherapy and Precision...
    Melanoma arises in the pigment producing cells (melanocytes) and is the deadliest of the skin cancers. It accounts for nearly 200,000 new cases of cancer each year worldwide and in the U.S. o...
    Speaker: Louise Perkins, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
    SEP 29, 2016 | 8:00 AM
    Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids
    Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles...
    PRESENTED BY:DATE:  September 29, 2016TIME:  8:00AM PT, 11AM ETInterest in 3D cell culture has grown exponentially in recent years, partially due to 3D cultures hel...
    Speaker: Dr. Glauco Souza, BS, MS, PhD
    Sponsored By: Cytiva
    5
    SEP 28, 2016 | 9:00 AM
    Signatures of protective NK cell responses
    Signatures of protective NK cell responses
    DATE:  September 28, 2016TIME:   9:00am Pacific time, 12:00pm Eastern timeNK cells play critical roles in immune defense and reproduction but remain the most poorly under...
    Speaker: Catherine Blish, MD, PhD , Michelle Poulin, PhD
    Sponsored By: Standard BioTools, Standard BioTools
    9 1
     
     
  • SEP 22, 2016 | 4:00 AM
    Cross-validation of Antibodies Using DigiWest
    Cross-validation of Antibodies Using DigiWest
    DATE: September 22, 2016 TIME:  4:00am PT, 7:00am ET, 11:00am GMT Here, we present a novel approach that combines the principles of the western blot (protein separation by SDS-PAGE; ...
    Speaker: Anthony Couvillon, PhD , Markus Templin, PhD
    Sponsored By: Cell Signaling Technology, Cell Signaling Technology
    3 2
    SEP 21, 2016 | 7:30 AM
    Using Data to Drive Automated Screening and Effective Reporting
    Using Data to Drive Automated Screening and Effective Reporting
    The most important job of automated biology is to produce results that moves your research forward. If automation only executes a program of pre-determined set of steps, the researcher is lef...
    Speaker: Tim Sherrill , Mike Kowalski, Ph.D.
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    6
    SEP 20, 2016 | 7:00 AM
    Advanced Analysis of Complex Cell Models with the New Operetta CLS HCA System
    Advanced Analysis of Complex Cell Models with the New Operetta...
    DATE: September 20th, 2016TIME:  7:00AM PST, 10:00AM ETThere is a growing trend towards developing in vitro cell models that recapitulate the in vivo environment in basic research,...
    Speaker: Jacob Tesdorpf, PhD , Joe Trask
    Sponsored By: Revvity
    25
    SEP 07, 2016 | 10:30 AM
    Exploring the Great Indoors: Relevance of the Built Environment to Human Health
    Exploring the Great Indoors: Relevance of the Built Environment...
    The human body is populated with trillions of microorganisms, collectively termed the human microbiome, that play vital roles in health including nutrition and metabolism, immune development,...
    Speaker: Roxana Hickey, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2016
    2
    SEP 07, 2016 | 6:00 AM
    Invisible Influence: How the indoor microbiome influences health
    Invisible Influence: How the indoor microbiome influences health
    The human race, like all macrobiological life, evolved in a sea of microbes. There was no way to keep the bacterial and archaeal hoards at bay, so instead life evolved mechanisms to live with...
    Speaker: Jack Gilbert, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2016
    2
     
     
  • AUG 30, 2016 | 8:00 AM
    iPSC Generation and Validation: Ensuring the best materials for downstream applications  iPSC Generation and Validation: Ensuring the best materials for downstream applications
    iPSC Generation and Validation: Ensuring the best materials for...
    The reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSC) has great potential of applications, not only in basic research, but also in drug screening and cell therapy. Wit...
    Speaker: Rene Quintanilla Jr.
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    0
    AUG 30, 2016 | 8:00 AM
    Improved differentiation of human pluripotent stem cell-derived neurons through reduction of progenitor proliferation: impact on downstream applications
    Improved differentiation of human pluripotent stem cell-derived...
    Neurons derived from human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are excellent resources for disease modeling and d...
    Speaker: David Kuninger, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    1
    AUG 30, 2016 | 8:00 AM
    Stem Cells for Regeneration and Rescue
    Stem Cells for Regeneration and Rescue
    Most discussions about pluripotent stem cells center around their promise for regenerative medicine.  The most remarkable quality of these cells is their ability to turn into all of the ...
    Speaker: Jeanne Loring
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    3
    AUG 30, 2016 | 8:00 AM
    Tissue engineering with human pluripotent stem cells
    Tissue engineering with human pluripotent stem cells
    One of the ultimate goals in Regenerative Medicine is the generation of pluripotent stem cells (PSCs) directly from somatic cells obtained from patients. Although major findings in the defini...
    Speaker: Nuria Montserrat, PhD
    Presented at: 4th Annual 24 Hours of Stem Cellsâ„¢ virtual event
    1
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