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    Cell Line Development

    Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.

    Webinars

    • NOV 17, 2016 | 9:00 AM
      From bench to BLA - a review of common regulatory questions
      From bench to BLA - a review of common regulatory questions
      This panel will discuss quality requirements of ancillary materials used in cell therapy manufacturing, relevant standards and how requirements vary by phase.  Participants will learn wh...
      Speaker: Joyce Frey-Vasconcells, PhD , Michael Mendicino, PhD , Kelli Tanzella, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      3
      NOV 17, 2016 | 8:30 AM
      Simultaneous, single-cell visualization of RNA and Protein
      Simultaneous, single-cell visualization of RNA and Protein
      DATE: November 17, 2016 TIME: 8:30am PT, 11:30am ET The complex interactions between transcription, translation, and post-translational modifications are hidden from view in typ...
      Speaker: Sara Becker-Catania, PhD
      Sponsored By: eBioscience Business Unit, Thermo Fisher Scientific, eBioscience Business Unit, Thermo Fisher Scientific
      4
      NOV 17, 2016 | 5:00 AM
      Partnering to advance regenerative medicine and cellular therapies
      Partnering to advance regenerative medicine and cellular therapies
      The Alliance for Regenerative Medicine (ARM) is the preeminent global advocate for regenerative and advanced therapies. ARM fosters research, development, investment and commercialization of ...
      Speaker: Michael Werner , Morrie Ruffin, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      4
      NOV 17, 2016 | 3:00 AM
      Genetic variability of ps-iPSC and related blood and fibroblasts - somatic mutations
      Genetic variability of ps-iPSC and related blood and fibroblasts...
      The generation of induced pluripotent stem cells (iPSCs) from adult easily accessible somatic tissues was introduced ten years ago. This technology has revolutionized our opportunities to stu...
      Speaker: KEYNOTE: Silke Rickert-Sperling, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      8 1
      NOV 16, 2016 | 6:00 PM
      Optimization of your PSCs workflow
      Optimization of your PSCs workflow
      Background: Applications and general workflow of PSCs Reprogramming with high efficiency and safety (Summary of current reprogramming methods, Introduction of Cytotune-iPS 2.0 Sendai Rep...
      Speaker: Jing He, PhD 何静 博士
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      3
      NOV 16, 2016 | 4:00 PM
      Evolving your media system for modern stem cell applications
      Evolving your media system for modern stem cell applications
      Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeut...
      Speaker: Rhonda Newman, PhD
      Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
      4
      NOV 15, 2016 | 9:00 AM
      Antigen-specific, poly-functional CD4+ T cells are required for vaccine-mediated protection in tularemia
      Antigen-specific, poly-functional CD4+ T cells are required for...
      DATE: November 15, 2016 TIME: 9:00 AM PT, 12:00 PM ET The virulent intracellular pathogen Francisella tularensis subsp. tularensis (Ftt) causes an acute, lethal diseas...
      Speaker: Lydia Roberts, PhD
      Sponsored By: Miltenyi Biotec, Miltenyi Biotec
      3
      NOV 10, 2016 | 7:30 AM
      Does your protein analysis toolbox need a refresh?
      Does your protein analysis toolbox need a refresh?
      DATE:  November 10, 2016 TIME:  7:30am PT, 10:30am ET  Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each migh...
      Speaker: Rich Jones, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      4
      NOV 09, 2016 | 9:00 AM
      A duplexed assay to measure GPCR-mediated calcium signaling and β-Arrestin recruitment from single well using FDSS/µCell functional screening system
      A duplexed assay to measure GPCR-mediated calcium signaling and...
      DATE: November 9th, 2016 TIME: 9:00 AM PT, 12:00 PM ET The screening and functional characterization of novel ligands for G-Protein Coupled Receptors (GPCR) remains a key focus in drug di...
      Speaker: Thierry Calmels, PhD
      Sponsored By: DiscoverX, DiscoverX
      3
      NOV 08, 2016 | 6:00 AM
      New Approaches to Antibody Validation Using Immunoprecipitation and Mass Spectrometry
      New Approaches to Antibody Validation Using Immunoprecipitation...
      Antibodies are used in a broad range of research and diagnostic applications for the enrichment, detection, and quantitation of proteins and their modifications.  Hundreds of thousands o...
      Speaker: John Rogers, PhD
      Presented at: Protein and Cell Analysis Education Series
      Sponsored By: Thermo Fisher Scientific
      9 2
      NOV 03, 2016 | 10:30 AM
      Rapid, Low-cost Tools for Global Health:  Using Cell-free Synthetic Biology for Zika Virus Detection and the Portable Manufacture of Therapeutics
      Rapid, Low-cost Tools for Global Health: Using Cell-free Synthetic...
      The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions.  In early 2016 we developed a pipe...
      Speaker: Keith Pardee, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      3
      NOV 02, 2016 | 1:30 PM
      A Platform to Digitize Biology: A Potential Pathway to Exponential Medicine
      A Platform to Digitize Biology: A Potential Pathway to Exponential...
      Today there are about 7B mobile phone worldwide and about 50,000 mobile health applications actively changing the landscape of how healthcare will be delivered in the next years. While numero...
      Speaker: John McDevitt, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      4
      NOV 02, 2016 | 10:30 AM
      Tough Targets. Simple Genotyping. Using HRM as a direct approach in research applications.
      Tough Targets. Simple Genotyping. Using HRM as a direct approach...
      Are there DNA targets in your lab that are difficult to genotype using standard PCR or sequencing?Do you ever need to genotype a single sample, and you don’t want to wait to batch sam...
      Speaker: Brian McNally, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
      2
      NOV 01, 2016 | 8:00 AM
      Development of selection strategies for Homologous Recombination Deficient (HRD) patients utilizing a Liquid Biopsy
      Development of selection strategies for Homologous Recombination...
      DATE: November 1, 2016 TIME: 8:00AM PT, 11:00AM ET Metastatic cancer is an evolving, heterogeneous disease that becomes more complex over time through the selection of sub-clonal tumor po...
      Speaker: Mark Landers
      Sponsored By: Epic Sciences, Epic Sciences , Epic Sciences
      4
      OCT 27, 2016 | 9:00 AM
      Achieving Reproducibility: Don't Let Antibodies be a Variable in Your Experiment
      Achieving Reproducibility: Don't Let Antibodies be a Variable...
      DATE:  October 27, 2016 TIME:  9:00am PT, 12:00pm ET The importance of antibodies as tools in scientific research studies cannot be understated, yet these reagents have increasi...
      Speaker: Anthony Couvillon, PhD
      Sponsored By: Cell Signaling Technology, Cell Signaling Technology
      10
      OCT 11, 2016 | 7:00 AM
      Cellular Applications for Image-based Cytometry on A Multimode Plate Reader
      Cellular Applications for Image-based Cytometry on A Multimode...
      Date: October 11th, 2016Time: 10:00AM EST, 4:00PM CEST, 3:00PM BST, 7:00AM PSTPlate readers first became available about 30 years ago and have become the workhorses of researc...
      Speaker: Frauke Haenel, PhD , Maria Kuzikov, MSc
      Sponsored By: Revvity
      8 1
      OCT 11, 2016 | 7:00 AM
      Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates
      Two Dimensional Liquid Chromatography Applied to the Characterization...
      DATE:  October 11, 2016 TIME:  7:00am PT, 10:00am ET Monoclonal antibodies (mAbs) have emerged as important therapeutics for the treatment of life-threatening diseases. The succ...
      Speaker: Koen Sandra, PhD
      Sponsored By: Agilent Genomics, Agilent Genomics
      5
      OCT 06, 2016 | 10:30 AM
      Precision Genomics and Precision Medicine for Immuno-Oncology
      Precision Genomics and Precision Medicine for Immuno-Oncology
      Tumors often evade immune destruction by adapting and circumventing immune checkpoints to create resistance.  This adaptive immunity can be reversed to stimulate the endogenous immune re...
      Speaker: Erin Newburn, MS, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      0
      OCT 06, 2016 | 9:00 AM
      Keynote Speaker - PD-1 cancer immunotherapy
      Keynote Speaker - PD-1 cancer immunotherapy
      The immune system is capable of recognizing tumors and eliminates many early malignant cells. However, tumors evolve to evade immune attack, and the tumor microenvironment is immunosuppressiv...
      Speaker: Gordon Freeman, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      OCT 05, 2016 | 10:30 AM
      Unraveling the Complexities of Immuno-Oncology with 3D Biology™ Technology
      Unraveling the Complexities of Immuno-Oncology with 3D Biology™...
      The ability to measure changes to DNA, RNA, and protein across cell types is crucial to understanding the immune response to the environment or therapeutic treatment. NanoString Technologies...
      Speaker: Joseph Beechem, PhD , Alessandra Cesano, MD, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      OCT 05, 2016 | 10:30 AM
      Immunotherapy of Cancer Using Genetically-Enhanced T Cells
      Immunotherapy of Cancer Using Genetically-Enhanced T Cells
      The immune system possesses significant cytotoxic potential. Stimulating natural immunity through vaccination has shown promising effects in some cancers; however, a number of barriers limit...
      Speaker: Michael Milone, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      OCT 05, 2016 | 6:00 AM
      Melanoma - View from the Frontlines of Immunotherapy and Precision Medicine
      Melanoma - View from the Frontlines of Immunotherapy and Precision...
      Melanoma arises in the pigment producing cells (melanocytes) and is the deadliest of the skin cancers. It accounts for nearly 200,000 new cases of cancer each year worldwide and in the U.S. o...
      Speaker: Louise Perkins, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
      2
      SEP 29, 2016 | 8:00 AM
      Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids
      Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles...
      PRESENTED BY:DATE:  September 29, 2016TIME:  8:00AM PT, 11AM ETInterest in 3D cell culture has grown exponentially in recent years, partially due to 3D cultures hel...
      Speaker: Dr. Glauco Souza, BS, MS, PhD
      Sponsored By: Cytiva
      5
      SEP 28, 2016 | 9:00 AM
      Signatures of protective NK cell responses
      Signatures of protective NK cell responses
      DATE:  September 28, 2016TIME:   9:00am Pacific time, 12:00pm Eastern timeNK cells play critical roles in immune defense and reproduction but remain the most poorly under...
      Speaker: Catherine Blish, MD, PhD , Michelle Poulin, PhD
      Sponsored By: Standard BioTools, Standard BioTools
      9 1
    NOV 17, 2016 | 9:00 AM
    From bench to BLA - a review of common regulatory questions
    From bench to BLA - a review of common regulatory questions
    This panel will discuss quality requirements of ancillary materials used in cell therapy manufacturing, relevant standards and how requirements vary by phase.  Participants will learn wh...
    Speaker: Joyce Frey-Vasconcells, PhD , Michael Mendicino, PhD , Kelli Tanzella, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    3
    NOV 17, 2016 | 8:30 AM
    Simultaneous, single-cell visualization of RNA and Protein
    Simultaneous, single-cell visualization of RNA and Protein
    DATE: November 17, 2016 TIME: 8:30am PT, 11:30am ET The complex interactions between transcription, translation, and post-translational modifications are hidden from view in typ...
    Speaker: Sara Becker-Catania, PhD
    Sponsored By: eBioscience Business Unit, Thermo Fisher Scientific, eBioscience Business Unit, Thermo Fisher Scientific
    4
    NOV 17, 2016 | 5:00 AM
    Partnering to advance regenerative medicine and cellular therapies
    Partnering to advance regenerative medicine and cellular therapies
    The Alliance for Regenerative Medicine (ARM) is the preeminent global advocate for regenerative and advanced therapies. ARM fosters research, development, investment and commercialization of ...
    Speaker: Michael Werner , Morrie Ruffin, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    4
    NOV 17, 2016 | 3:00 AM
    Genetic variability of ps-iPSC and related blood and fibroblasts - somatic mutations
    Genetic variability of ps-iPSC and related blood and fibroblasts...
    The generation of induced pluripotent stem cells (iPSCs) from adult easily accessible somatic tissues was introduced ten years ago. This technology has revolutionized our opportunities to stu...
    Speaker: KEYNOTE: Silke Rickert-Sperling, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    8 1
    NOV 16, 2016 | 6:00 PM
    Optimization of your PSCs workflow
    Optimization of your PSCs workflow
    Background: Applications and general workflow of PSCs Reprogramming with high efficiency and safety (Summary of current reprogramming methods, Introduction of Cytotune-iPS 2.0 Sendai Rep...
    Speaker: Jing He, PhD 何静 博士
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    3
     
     
  • NOV 16, 2016 | 4:00 PM
    Evolving your media system for modern stem cell applications
    Evolving your media system for modern stem cell applications
    Culture systems for pluripotent stem cell (PSC) expansion enable generation of a nearly unlimited pool of cells for downstream differentiation, disease modeling, drug discovery, and therapeut...
    Speaker: Rhonda Newman, PhD
    Presented at: 4th Annual 24 Hours of Stem Cells™ virtual event
    4
    NOV 15, 2016 | 9:00 AM
    Antigen-specific, poly-functional CD4+ T cells are required for vaccine-mediated protection in tularemia
    Antigen-specific, poly-functional CD4+ T cells are required for...
    DATE: November 15, 2016 TIME: 9:00 AM PT, 12:00 PM ET The virulent intracellular pathogen Francisella tularensis subsp. tularensis (Ftt) causes an acute, lethal diseas...
    Speaker: Lydia Roberts, PhD
    Sponsored By: Miltenyi Biotec, Miltenyi Biotec
    3
    NOV 10, 2016 | 7:30 AM
    Does your protein analysis toolbox need a refresh?
    Does your protein analysis toolbox need a refresh?
    DATE:  November 10, 2016 TIME:  7:30am PT, 10:30am ET  Does your protein analysis toolbox need a refresh? Do you have a firm grasp on the tools available and when each migh...
    Speaker: Rich Jones, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    4
    NOV 09, 2016 | 9:00 AM
    A duplexed assay to measure GPCR-mediated calcium signaling and β-Arrestin recruitment from single well using FDSS/µCell functional screening system
    A duplexed assay to measure GPCR-mediated calcium signaling and...
    DATE: November 9th, 2016 TIME: 9:00 AM PT, 12:00 PM ET The screening and functional characterization of novel ligands for G-Protein Coupled Receptors (GPCR) remains a key focus in drug di...
    Speaker: Thierry Calmels, PhD
    Sponsored By: DiscoverX, DiscoverX
    3
    NOV 08, 2016 | 6:00 AM
    New Approaches to Antibody Validation Using Immunoprecipitation and Mass Spectrometry
    New Approaches to Antibody Validation Using Immunoprecipitation...
    Antibodies are used in a broad range of research and diagnostic applications for the enrichment, detection, and quantitation of proteins and their modifications.  Hundreds of thousands o...
    Speaker: John Rogers, PhD
    Presented at: Protein and Cell Analysis Education Series
    Sponsored By: Thermo Fisher Scientific
    9 2
     
     
  • NOV 03, 2016 | 10:30 AM
    Rapid, Low-cost Tools for Global Health:  Using Cell-free Synthetic Biology for Zika Virus Detection and the Portable Manufacture of Therapeutics
    Rapid, Low-cost Tools for Global Health: Using Cell-free Synthetic...
    The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions.  In early 2016 we developed a pipe...
    Speaker: Keith Pardee, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    3
    NOV 02, 2016 | 1:30 PM
    A Platform to Digitize Biology: A Potential Pathway to Exponential Medicine
    A Platform to Digitize Biology: A Potential Pathway to Exponential...
    Today there are about 7B mobile phone worldwide and about 50,000 mobile health applications actively changing the landscape of how healthcare will be delivered in the next years. While numero...
    Speaker: John McDevitt, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    4
    NOV 02, 2016 | 10:30 AM
    Tough Targets. Simple Genotyping. Using HRM as a direct approach in research applications.
    Tough Targets. Simple Genotyping. Using HRM as a direct approach...
    Are there DNA targets in your lab that are difficult to genotype using standard PCR or sequencing?Do you ever need to genotype a single sample, and you don’t want to wait to batch sam...
    Speaker: Brian McNally, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2016
    2
    NOV 01, 2016 | 8:00 AM
    Development of selection strategies for Homologous Recombination Deficient (HRD) patients utilizing a Liquid Biopsy
    Development of selection strategies for Homologous Recombination...
    DATE: November 1, 2016 TIME: 8:00AM PT, 11:00AM ET Metastatic cancer is an evolving, heterogeneous disease that becomes more complex over time through the selection of sub-clonal tumor po...
    Speaker: Mark Landers
    Sponsored By: Epic Sciences, Epic Sciences , Epic Sciences
    4
    OCT 27, 2016 | 9:00 AM
    Achieving Reproducibility: Don't Let Antibodies be a Variable in Your Experiment
    Achieving Reproducibility: Don't Let Antibodies be a Variable...
    DATE:  October 27, 2016 TIME:  9:00am PT, 12:00pm ET The importance of antibodies as tools in scientific research studies cannot be understated, yet these reagents have increasi...
    Speaker: Anthony Couvillon, PhD
    Sponsored By: Cell Signaling Technology, Cell Signaling Technology
    10
     
     
  • OCT 11, 2016 | 7:00 AM
    Cellular Applications for Image-based Cytometry on A Multimode Plate Reader
    Cellular Applications for Image-based Cytometry on A Multimode...
    Date: October 11th, 2016Time: 10:00AM EST, 4:00PM CEST, 3:00PM BST, 7:00AM PSTPlate readers first became available about 30 years ago and have become the workhorses of researc...
    Speaker: Frauke Haenel, PhD , Maria Kuzikov, MSc
    Sponsored By: Revvity
    8 1
    OCT 11, 2016 | 7:00 AM
    Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates
    Two Dimensional Liquid Chromatography Applied to the Characterization...
    DATE:  October 11, 2016 TIME:  7:00am PT, 10:00am ET Monoclonal antibodies (mAbs) have emerged as important therapeutics for the treatment of life-threatening diseases. The succ...
    Speaker: Koen Sandra, PhD
    Sponsored By: Agilent Genomics, Agilent Genomics
    5
    OCT 06, 2016 | 10:30 AM
    Precision Genomics and Precision Medicine for Immuno-Oncology
    Precision Genomics and Precision Medicine for Immuno-Oncology
    Tumors often evade immune destruction by adapting and circumventing immune checkpoints to create resistance.  This adaptive immunity can be reversed to stimulate the endogenous immune re...
    Speaker: Erin Newburn, MS, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    0
    OCT 06, 2016 | 9:00 AM
    Keynote Speaker - PD-1 cancer immunotherapy
    Keynote Speaker - PD-1 cancer immunotherapy
    The immune system is capable of recognizing tumors and eliminates many early malignant cells. However, tumors evolve to evade immune attack, and the tumor microenvironment is immunosuppressiv...
    Speaker: Gordon Freeman, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
    OCT 05, 2016 | 10:30 AM
    Unraveling the Complexities of Immuno-Oncology with 3D Biology™ Technology
    Unraveling the Complexities of Immuno-Oncology with 3D Biology™...
    The ability to measure changes to DNA, RNA, and protein across cell types is crucial to understanding the immune response to the environment or therapeutic treatment. NanoString Technologies...
    Speaker: Joseph Beechem, PhD , Alessandra Cesano, MD, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
     
     
  • OCT 05, 2016 | 10:30 AM
    Immunotherapy of Cancer Using Genetically-Enhanced T Cells
    Immunotherapy of Cancer Using Genetically-Enhanced T Cells
    The immune system possesses significant cytotoxic potential. Stimulating natural immunity through vaccination has shown promising effects in some cancers; however, a number of barriers limit...
    Speaker: Michael Milone, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
    OCT 05, 2016 | 6:00 AM
    Melanoma - View from the Frontlines of Immunotherapy and Precision Medicine
    Melanoma - View from the Frontlines of Immunotherapy and Precision...
    Melanoma arises in the pigment producing cells (melanocytes) and is the deadliest of the skin cancers. It accounts for nearly 200,000 new cases of cancer each year worldwide and in the U.S. o...
    Speaker: Louise Perkins, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2016
    2
    SEP 29, 2016 | 8:00 AM
    Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids
    Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles...
    PRESENTED BY:DATE:  September 29, 2016TIME:  8:00AM PT, 11AM ETInterest in 3D cell culture has grown exponentially in recent years, partially due to 3D cultures hel...
    Speaker: Dr. Glauco Souza, BS, MS, PhD
    Sponsored By: Cytiva
    5
    SEP 28, 2016 | 9:00 AM
    Signatures of protective NK cell responses
    Signatures of protective NK cell responses
    DATE:  September 28, 2016TIME:   9:00am Pacific time, 12:00pm Eastern timeNK cells play critical roles in immune defense and reproduction but remain the most poorly under...
    Speaker: Catherine Blish, MD, PhD , Michelle Poulin, PhD
    Sponsored By: Standard BioTools, Standard BioTools
    9 1
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