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    Cell Line Development

    Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.

    Webinars

    • AUG 22, 2019 | 10:30 AM
      Generating Authentic Research Experiences of Forensic-Based Projects in an Undergraduate Classroom Laboratory
      Generating Authentic Research Experiences of Forensic-Based Projects...
      DNA profiling tools to teach undergraduate students about forensics generally utilize the PV92 Alu and D1S80 VNTR markers, but are both limited in scope. In contrast, advanced profiling syste...
      Speaker: David Kass, PhD
      Presented at: Forensics & Toxicology 2019
      2
      AUG 15, 2019 | 9:00 AM
      Understanding and ameliorating radiation-induced damage to oligodendrocytes
      Understanding and ameliorating radiation-induced damage to oligodendrocytes
      DATE: August 15, 2019TIME: 9:00am PDT, 12:00pm EDT Radiation therapy is a critical tool for the treatment of brain tumors, however, exposure to high doses of ionizing radiation...
      Speaker: Alexandra Taraboletti, PHD
      Sponsored By: Thermo Fisher Scientific/Gibco
      6
      JUL 31, 2019 | 9:00 AM
      Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
      Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
      DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
      Speaker: Elizabeth Delery, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      7 1
      MAY 23, 2019 | 9:00 AM
      Doing More with Less: Stem Cells Regulate Their Fate by Altering Their Stiffness
      Doing More with Less: Stem Cells Regulate Their Fate by Altering...
      DATE:  May 23, 2019TIME:   9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
      Speaker: Melika Sarem, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      7
      APR 23, 2019 | 9:00 AM
      Liquid Based Microbiology: Simplify Sample Collection and Transport to Improve Patient Care
      Liquid Based Microbiology: Simplify Sample Collection and Transport...
      DATE: April 23, 2019TIME: 9:00am PDT, 12:00pm EDT ABSTRACT: Learning Objectives: List benefits of liquid based collection and transport systems...
      Speaker: Susan Sharp, Ph.D., DABMM, FAAM
      Sponsored By: COPAN Diagnostics
      20
      APR 02, 2019 | 3:00 PM
      L'interférence de la Biotine sur les immunoanalyses
      L'interférence de la Biotine sur les immunoanalyses
      DATE: April 2, 2019TIME: 3:00pm CEST Dans ce webinaire, Marie Piketty rappelle que depuis quelques années, plusieurs cas d'interférences provoquées par l...
      Speaker: Marie Piketty, Biology MSC
      Sponsored By: Abbott
      6 1
      MAR 28, 2019 | 12:00 PM
      The Intersection of Nutrition, Microbiome and Medical Cannabis
      The Intersection of Nutrition, Microbiome and Medical Cannabis
      Whole food plant-based diets and medical cannabis have shared and continues to share similar levels of scrutiny, doubt, and stigma by the traditional medical community. One reason for the med...
      Speaker: Debra Kimless, MD
      Presented at: Cannabis Sciences Virtual Event Series 2019
      6
      FEB 27, 2019 | 12:00 PM
      Evaluating Biomarkers for Response to Neoadjuvant Immune Checkpoint Blockade in Melanoma
      Evaluating Biomarkers for Response to Neoadjuvant Immune Checkpoint...
      Surgery is the first line of treatment for Stage III melanoma. Often adjuvant therapy is administered post-surgery, which can include weeks of radiation, chemotherapy, targeted drug therapy,...
      Speaker: Sarah Warren, PhD
      Presented at: Drug Discovery Virtual Event Series 2019
      Sponsored By: NanoString Technologies
      1
      FEB 27, 2019 | 8:00 AM
      Prepare for success: GMP cell banks as part of a staged, standardized, platform-style cell production process
      Prepare for success: GMP cell banks as part of a staged, standardized,...
      DATE:  February 27, 2019TIME:  8:00am PST Pluripotent stem cells (PSCs) can form any tissue or cell in the body, and are the ideal starting material to manufacture...
      Speaker: Benjamin Fryer
      Sponsored By: Thermo Fisher Scientific/Gibco
      9
      JAN 31, 2019 | 1:30 PM
      Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications
      Standardization of Cell Viability Assays in Primary Cells as a...
      Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and ne...
      Speaker: Simon Kaja, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      2
      JAN 31, 2019 | 12:00 PM
      From Start to Finish, Bio-Techne Solutions for Bioprocessing
      From Start to Finish, Bio-Techne Solutions for Bioprocessing
      As the pace of biotherapeutic approvals increases, so does the pressure on makers of new biologics to complete their development processes faster and more efficiently. The success of the drug...
      Speaker: Christopher Heger, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      Sponsored By: ACD - A Bio-Techne Brand
      3 1
      JAN 31, 2019 | 10:30 AM
      Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries to Improve Process Efficiencies
      Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries...
      Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-t...
      Speaker: Buzz Lobbezoo
      Presented at: BioProcessing Virtual Event Series 2019
      Sponsored By: Astrea Bioseparations
      1
      JAN 31, 2019 | 10:30 AM
      Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications
      Overcoming Limitations of Conventional Tag Systems - Strep-Tactin®...
      The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity a...
      Speaker: Dennis Karthaus, MS
      Presented at: BioProcessing Virtual Event Series 2019
      2
      JAN 31, 2019 | 9:00 AM
      Streamlining the Biologics Development Process: From Transient to Stable Production in the ExpiCHO System
      Streamlining the Biologics Development Process: From Transient...
      The biologics development process is fraught with risks from pre-clinical studies through clinical evaluation. Prominent among these risks are changes in the critical quality attributes of th...
      Speaker: Jon Zmuda, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      1
      JAN 31, 2019 | 7:30 AM
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
      Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
      Speaker: A.C. Matin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      1
      JAN 31, 2019 | 6:00 AM
      Overcoming Challenges in EuCODE Cell Culture Platform Development
      Overcoming Challenges in EuCODE Cell Culture Platform Development
      Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the...
      Speaker: Weimin Lin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      3
      DEC 13, 2018 | 9:00 AM
      Optimizing Treatment Options for HER2+/ ER+ Breast Cancer using Models of Endocrine Resistance
      Optimizing Treatment Options for HER2+/ ER+ Breast Cancer using...
      DATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...
      Speaker: Hillary Stires, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      3
      DEC 11, 2018 | 10:00 AM
      Combining Reichert's SPR Systems With Other Informative Techniques
      Combining Reichert's SPR Systems With Other Informative Techniques
      DATE: December 11, 2018TIME: 11:00 PSTThis webinar will focus on how Reichert's Surface Plasmon Resonance (SPR) systems can generate additional valuable information that is not...
      Speaker: Phillip Page, PhD , Mary Murphy, PhD
      Sponsored By: Ametek
      3
      NOV 15, 2018 | 12:00 PM
      Using human stem cells to model neocortical gyration phenotypes
      Using human stem cells to model neocortical gyration phenotypes
      Neuronal migration defects, including pachygyria, are among the most severe developmental brain defects in humans. Using human genetics approaches, we recently identified bi-allelic truncatin...
      Speaker: Ashleigh Schaffer
      Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
      6 1
      NOV 15, 2018 | 10:00 AM
      Applications of Brain-Model Technology
      Applications of Brain-Model Technology
      The complexity of the human brain, with thousands of neuronal types, permits the development of sophisticated behavioral repertoires, such as language, tool use, self-awareness, symbolic thou...
      Speaker: Alysson Muotri
      Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
      4
      NOV 15, 2018 | 10:00 AM
      Multiplex fluorescence immunohistochemistry using the Ultivue InSituPlex platform on the Leica Biosystems BOND RX
      Multiplex fluorescence immunohistochemistry using the Ultivue...
      DATE:  November 15, 2018TIME:  10:00am PT, 1:00pm ET Multiplex fluorescence immunohistochemistry offers a window into the biology of human disease, enabling the ana...
      Speaker: Traci DeGeer, BS, HT (ASCP) HTL, QIHC , Alexander Klimowicz
      Sponsored By: Leica Biosystems
      6 1
      NOV 15, 2018 | 4:00 AM
      Generation and application of 3D-organoid cell culture models using human induced pluripotent stem cells (iPSCs) for drug discovery
      Generation and application of 3D-organoid cell culture models...
      Development of physiologically relevant cellular models, with strong translatability to human pathophysiology, is critical for identification and validation of novel therapeutic targets. Cell...
      Speaker: Ryan Hicks
      Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
      10 2
      NOV 15, 2018 | 2:00 AM
      Towards commercial manufacture of pluripotent stem cell derived therapies
      Towards commercial manufacture of pluripotent stem cell derived...
      In contrast to autologous cell therapies, allogeneic therapies do not require collection of starting material from patients and could; be manufactured in larger batches, be made available &ld...
      Speaker: Rhys Macown
      Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
      10
      NOV 14, 2018 | 3:00 PM
      Modeling Inflammation and Fibrosis in Humans with PSC-derived Steatohepatitis Liver Organoids
      Modeling Inflammation and Fibrosis in Humans with PSC-derived...
      Neuronal migration defects, including pachygyria, are among the most severe developmental brain defects in humans. Using human genetics approaches, we recently identified bi-allelic truncatin...
      Speaker: Takanori Takebe
      Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
      8
    AUG 22, 2019 | 10:30 AM
    Generating Authentic Research Experiences of Forensic-Based Projects in an Undergraduate Classroom Laboratory
    Generating Authentic Research Experiences of Forensic-Based Projects...
    DNA profiling tools to teach undergraduate students about forensics generally utilize the PV92 Alu and D1S80 VNTR markers, but are both limited in scope. In contrast, advanced profiling syste...
    Speaker: David Kass, PhD
    Presented at: Forensics & Toxicology 2019
    2
    AUG 15, 2019 | 9:00 AM
    Understanding and ameliorating radiation-induced damage to oligodendrocytes
    Understanding and ameliorating radiation-induced damage to oligodendrocytes
    DATE: August 15, 2019TIME: 9:00am PDT, 12:00pm EDT Radiation therapy is a critical tool for the treatment of brain tumors, however, exposure to high doses of ionizing radiation...
    Speaker: Alexandra Taraboletti, PHD
    Sponsored By: Thermo Fisher Scientific/Gibco
    6
    JUL 31, 2019 | 9:00 AM
    Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
    Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model
    DATE: July 31, 2019TIME: 9:00am PT, 12:00pm ET The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricle...
    Speaker: Elizabeth Delery, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    7 1
    MAY 23, 2019 | 9:00 AM
    Doing More with Less: Stem Cells Regulate Their Fate by Altering Their Stiffness
    Doing More with Less: Stem Cells Regulate Their Fate by Altering...
    DATE:  May 23, 2019TIME:   9:00am PDT, 12:00pm EDT Although mesenchymal stem/stromal cells (MSCs) chondrogenic differentiation has been thoroughly investigated...
    Speaker: Melika Sarem, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    7
    APR 23, 2019 | 9:00 AM
    Liquid Based Microbiology: Simplify Sample Collection and Transport to Improve Patient Care
    Liquid Based Microbiology: Simplify Sample Collection and Transport...
    DATE: April 23, 2019TIME: 9:00am PDT, 12:00pm EDT ABSTRACT: Learning Objectives: List benefits of liquid based collection and transport systems...
    Speaker: Susan Sharp, Ph.D., DABMM, FAAM
    Sponsored By: COPAN Diagnostics
    20
     
     
  • APR 02, 2019 | 3:00 PM
    L'interférence de la Biotine sur les immunoanalyses
    L'interférence de la Biotine sur les immunoanalyses
    DATE: April 2, 2019TIME: 3:00pm CEST Dans ce webinaire, Marie Piketty rappelle que depuis quelques années, plusieurs cas d'interférences provoquées par l...
    Speaker: Marie Piketty, Biology MSC
    Sponsored By: Abbott
    6 1
    MAR 28, 2019 | 12:00 PM
    The Intersection of Nutrition, Microbiome and Medical Cannabis
    The Intersection of Nutrition, Microbiome and Medical Cannabis
    Whole food plant-based diets and medical cannabis have shared and continues to share similar levels of scrutiny, doubt, and stigma by the traditional medical community. One reason for the med...
    Speaker: Debra Kimless, MD
    Presented at: Cannabis Sciences Virtual Event Series 2019
    6
    FEB 27, 2019 | 12:00 PM
    Evaluating Biomarkers for Response to Neoadjuvant Immune Checkpoint Blockade in Melanoma
    Evaluating Biomarkers for Response to Neoadjuvant Immune Checkpoint...
    Surgery is the first line of treatment for Stage III melanoma. Often adjuvant therapy is administered post-surgery, which can include weeks of radiation, chemotherapy, targeted drug therapy,...
    Speaker: Sarah Warren, PhD
    Presented at: Drug Discovery Virtual Event Series 2019
    Sponsored By: NanoString Technologies
    1
    FEB 27, 2019 | 8:00 AM
    Prepare for success: GMP cell banks as part of a staged, standardized, platform-style cell production process
    Prepare for success: GMP cell banks as part of a staged, standardized,...
    DATE:  February 27, 2019TIME:  8:00am PST Pluripotent stem cells (PSCs) can form any tissue or cell in the body, and are the ideal starting material to manufacture...
    Speaker: Benjamin Fryer
    Sponsored By: Thermo Fisher Scientific/Gibco
    9
    JAN 31, 2019 | 1:30 PM
    Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications
    Standardization of Cell Viability Assays in Primary Cells as a...
    Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and ne...
    Speaker: Simon Kaja, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    2
     
     
  • JAN 31, 2019 | 12:00 PM
    From Start to Finish, Bio-Techne Solutions for Bioprocessing
    From Start to Finish, Bio-Techne Solutions for Bioprocessing
    As the pace of biotherapeutic approvals increases, so does the pressure on makers of new biologics to complete their development processes faster and more efficiently. The success of the drug...
    Speaker: Christopher Heger, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    Sponsored By: ACD - A Bio-Techne Brand
    3 1
    JAN 31, 2019 | 10:30 AM
    Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries to Improve Process Efficiencies
    Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries...
    Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-t...
    Speaker: Buzz Lobbezoo
    Presented at: BioProcessing Virtual Event Series 2019
    Sponsored By: Astrea Bioseparations
    1
    JAN 31, 2019 | 10:30 AM
    Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications
    Overcoming Limitations of Conventional Tag Systems - Strep-Tactin®...
    The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity a...
    Speaker: Dennis Karthaus, MS
    Presented at: BioProcessing Virtual Event Series 2019
    2
    JAN 31, 2019 | 9:00 AM
    Streamlining the Biologics Development Process: From Transient to Stable Production in the ExpiCHO System
    Streamlining the Biologics Development Process: From Transient...
    The biologics development process is fraught with risks from pre-clinical studies through clinical evaluation. Prominent among these risks are changes in the critical quality attributes of th...
    Speaker: Jon Zmuda, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    1
    JAN 31, 2019 | 7:30 AM
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
    Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
    Speaker: A.C. Matin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    1
     
     
  • JAN 31, 2019 | 6:00 AM
    Overcoming Challenges in EuCODE Cell Culture Platform Development
    Overcoming Challenges in EuCODE Cell Culture Platform Development
    Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the...
    Speaker: Weimin Lin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    3
    DEC 13, 2018 | 9:00 AM
    Optimizing Treatment Options for HER2+/ ER+ Breast Cancer using Models of Endocrine Resistance
    Optimizing Treatment Options for HER2+/ ER+ Breast Cancer using...
    DATE: December 13 15,2018TIME: 09:00am PST, 12:00pm EST Breast cancers are classified into three main subtypes according to their receptor status: estrogen receptor-positive...
    Speaker: Hillary Stires, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    3
    DEC 11, 2018 | 10:00 AM
    Combining Reichert's SPR Systems With Other Informative Techniques
    Combining Reichert's SPR Systems With Other Informative Techniques
    DATE: December 11, 2018TIME: 11:00 PSTThis webinar will focus on how Reichert's Surface Plasmon Resonance (SPR) systems can generate additional valuable information that is not...
    Speaker: Phillip Page, PhD , Mary Murphy, PhD
    Sponsored By: Ametek
    3
    NOV 15, 2018 | 12:00 PM
    Using human stem cells to model neocortical gyration phenotypes
    Using human stem cells to model neocortical gyration phenotypes
    Neuronal migration defects, including pachygyria, are among the most severe developmental brain defects in humans. Using human genetics approaches, we recently identified bi-allelic truncatin...
    Speaker: Ashleigh Schaffer
    Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
    6 1
    NOV 15, 2018 | 10:00 AM
    Applications of Brain-Model Technology
    Applications of Brain-Model Technology
    The complexity of the human brain, with thousands of neuronal types, permits the development of sophisticated behavioral repertoires, such as language, tool use, self-awareness, symbolic thou...
    Speaker: Alysson Muotri
    Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
    4
     
     
  • NOV 15, 2018 | 10:00 AM
    Multiplex fluorescence immunohistochemistry using the Ultivue InSituPlex platform on the Leica Biosystems BOND RX
    Multiplex fluorescence immunohistochemistry using the Ultivue...
    DATE:  November 15, 2018TIME:  10:00am PT, 1:00pm ET Multiplex fluorescence immunohistochemistry offers a window into the biology of human disease, enabling the ana...
    Speaker: Traci DeGeer, BS, HT (ASCP) HTL, QIHC , Alexander Klimowicz
    Sponsored By: Leica Biosystems
    6 1
    NOV 15, 2018 | 4:00 AM
    Generation and application of 3D-organoid cell culture models using human induced pluripotent stem cells (iPSCs) for drug discovery
    Generation and application of 3D-organoid cell culture models...
    Development of physiologically relevant cellular models, with strong translatability to human pathophysiology, is critical for identification and validation of novel therapeutic targets. Cell...
    Speaker: Ryan Hicks
    Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
    10 2
    NOV 15, 2018 | 2:00 AM
    Towards commercial manufacture of pluripotent stem cell derived therapies
    Towards commercial manufacture of pluripotent stem cell derived...
    In contrast to autologous cell therapies, allogeneic therapies do not require collection of starting material from patients and could; be manufactured in larger batches, be made available &ld...
    Speaker: Rhys Macown
    Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
    10
    NOV 14, 2018 | 3:00 PM
    Modeling Inflammation and Fibrosis in Humans with PSC-derived Steatohepatitis Liver Organoids
    Modeling Inflammation and Fibrosis in Humans with PSC-derived...
    Neuronal migration defects, including pachygyria, are among the most severe developmental brain defects in humans. Using human genetics approaches, we recently identified bi-allelic truncatin...
    Speaker: Takanori Takebe
    Presented at: 6th annual 24 Hours of Stem Cellsâ„¢ virtual event
    8
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