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    Cell Line Development

    Cell Line Development: Stable cell lines are widely used in a number of important applications including biologics (e.g. recombinant protein and monoclonal antibody) production, drug screening, and gene functional studies. The process of developing stable cell lines often starts with transfecting selected host cells, typically CHO or HEK 293 cells, with desired plasmids. After transfection, researchers then screen and quantify high-expressing clones. Once these high producers are identified, the cell lines and/or the proteins produced by the cells are validated. The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high-throughput, automated solutions for such efforts.

    Webinars

    • MAR 05, 2020 | 9:00 AM
      An Update on H. pylori testing with a focus on the Houston Consensus recommendations
      An Update on H. pylori testing with a focus on the Houston Consensus...
      Recommendations for key factors in testing and diagnosis of H. pylori infections to include information on appropriate patients for H.pylori testing, current testing options, post treatment...
      Speaker: David Graham, MD
      8
      FEB 25, 2020 | 10:00 AM
      Avances en la Tecnología del Diferencial de Glóbulos Blancos
      Avances en la Tecnología del Diferencial de Glóbulos Blancos
      DATE: 25 Febrero 2020 TIME: 13:00 COT, 10:00 PST Los desarrollos tecnológicos en analizadores automatizados de Hematología continúan transformando el laboratorio de Hema...
      Speaker: Don Wright
      Sponsored By: Abbott
      5 1
      FEB 05, 2020 | 8:00 AM
      C.E. CREDITS
      Emerging Issues in the Coagulation Laboratory
      Emerging Issues in the Coagulation Laboratory
      Learn how new hemophilia and anticoagulant therapies impact coagulation assays and clinical decisions in our educational session....
      Speaker: Donna Castellone, MS, MASCP, MT(ASCP) SH
      Presented at: Stago EdVantage Virtual University
      Sponsored By: Diagnostica Stago, Inc.
      10 1
      JAN 23, 2020 | 9:00 AM
      A Workflow for Knock-in Genome Editing: Simplified
      A Workflow for Knock-in Genome Editing: Simplified
      DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
      Speaker: Matthew Poling, PhD
      Sponsored By: Thermo Fisher Scientific
      17
      JAN 14, 2020 | 9:00 AM
      Fecal Elastase-1 - a Biomarker for Pancreatic Exocrine Insufficiency
      Fecal Elastase-1 - a Biomarker for Pancreatic Exocrine Insufficiency
      Fecal elastase-1 – a biomarker for pancreatic exocrine insufficiency(EPI) continues to gain traction as an ideal biomarker for assessing EPI. This presentation will include a review of...
      Speaker: Roy Sherwood, BSc, MSC, DPhil
      Sponsored By: Diasorin S.p.A.
      5
      JAN 01, 2020 | 6:00 AM
      Microphysiological Systems for Human Focused Drug Discovery
      Microphysiological Systems for Human Focused Drug Discovery
      Microphysiological systems (MPS), also known as organ-on-chips, are small scale in vitro cell cultures which mimic facets of tissue or organ level function. MPS frequently utilise primary hu...
      Speaker: Tomasz Kostrzewski, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2020
      3
      JAN 01, 2020 | 6:00 AM
      Generation of expanded primary liver cells
      Generation of expanded primary liver cells
      The use of primary cells in vitro is compromised by the limited quantity of cells that can be isolated from one donor, a lack of or very restricted proliferation capacity (e.g. hepatocytes)...
      Speaker: Astrid Nörenberg, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2020
      1
      JAN 01, 2020 | 6:00 AM
      Organoids: A Revolutionary New Tool for Drug Screening
      Organoids: A Revolutionary New Tool for Drug Screening
      “The poor translatability of early-stage preclinical models is a major setback in oncology drug development. Immortalized cell lines, that are extensively used in drug screens, undergo...
      Speaker: Rekha Pal, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2020
      1
      DEC 17, 2019 | 8:00 AM
      Single-Cell Phosphoprotein Analysis with CyTOF: From Training to Immuno-Oncology Clinical Research
      Single-Cell Phosphoprotein Analysis with CyTOF: From Training...
      DATE: December 17, 2019TIME: 8:00am PSTMass cytometry instruments simultaneously measure >50 cellular features, with a typical experiment including millions of cells. Dysregulated...
      Speaker: Jonathan Irish, PhD , Caroline E. Roe, BS
      Sponsored By: Standard BioTools
      7 2
      DEC 11, 2019 | 7:00 AM
      A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
      A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis...
      DATE: December 11, 2019TIME: 7:00am PST, 10:00am EST An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key...
      Speaker: Colleen Reed
      Sponsored By: SCIEX
      7
      DEC 10, 2019 | 8:00 AM
      Expi293: New Tools for Structural Biology and Difficult to Express Proteins
      Expi293: New Tools for Structural Biology and Difficult to Express...
      DATE: December 10, 2019TIME: 8:00am PST, 11:00am EST In this presentation, we demonstrate the performance of a new range of Expi293 components, including engineered Expi293 cell...
      Speaker: Jon Zmuda, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      7 1
      DEC 04, 2019 | 7:00 AM
      Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
      Improve your reproducibility - Liquid Handling is a crucial factor...
      DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
      Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
      Sponsored By: Eppendorf
      12
      NOV 15, 2019 | 8:00 AM
      Exosomes and super-resolution microscopy
      Exosomes and super-resolution microscopy
      DATE: November 15, 2019TIME: 8:00am PST, 11:00am EST Exosomes are small vesicles, ranging from 30-120 nanometers in diameter, secreted from cells throughout the human body. The...
      Speaker: Ryan McNamara, PhD
      Sponsored By: ONI
      8
      NOV 14, 2019 | 6:00 AM
      High Resolution View of D4Z4 Repeat Regions for Studying Facioscapulohumeral Muscular Dystrophy (FSHD) Using Whole Genome Optical Mapping
      High Resolution View of D4Z4 Repeat Regions for Studying Facioscapulohumeral...
      Facioscapulohumeral muscular dystrophy (FSHD), one of the most common forms of progressive muscular dystrophies and is often conventionally diagnosed by Southern blot analysis. The accurate d...
      Speaker: Alka Chaubey, PhD, FACMG
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2019
      Sponsored By: Bionano
      2
      NOV 12, 2019 | 10:00 AM
      Leveraging single cell copy number to resolve tumor clonality
      Leveraging single cell copy number to resolve tumor clonality
      DATE: November 12, 2019TIME: 10:00am PSTDirect measurement of copy number by droplet-based shallow sequencing of genomic DNA has the potential to provide new insights into tumor heterog...
      Speaker: Dr. David Craig
      Sponsored By: 10x Genomics
      9 1
      OCT 31, 2019 | 9:00 AM
      Investigation of Endothelial Cell Viability and Growth on 3D printed GelMa Vascular Networks
      Investigation of Endothelial Cell Viability and Growth on 3D printed...
      DATE: October 31, 2019TIME: 9:00am PDT, 12:00pm EDT INTRODUCTION: A major limitation for the development of 3D engineered tissues is the absence of viable and perfusable...
      Speaker: Chrisoula Chappell
      Sponsored By: Thermo Fisher Scientific/Gibco
      8
      OCT 22, 2019 | 9:00 AM
      Preserving Data Integrity: 21 CFR Part 11 Compliance and Osmolality as a Process Parameter
      Preserving Data Integrity: 21 CFR Part 11 Compliance and Osmolality...
      DATE: October 22, 2019 TIME: 9:00am PDT, 12:00PM EDT...
      Speaker: Angela Bazigos , Kristeena Wright, PhD , Shweta Nair
      Sponsored By: Advanced Instruments
      10 1
      OCT 10, 2019 | 6:00 AM
      Highly-efficient and -specific genome editing with Cas9 nickase ribonucleoproteins in Primary T-cells
      Highly-efficient and -specific genome editing with Cas9 nickase...
      In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases wh...
      Speaker: Jacob Lamberth
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2019
      Sponsored By: MilliporeSigma
      1
      OCT 03, 2019 | 12:00 PM
      Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency
      Gene Editing Success: Considerations for maximizing your CRISPR...
      CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out prot...
      Speaker: Ryan Donnelly
      Presented at: CRISPR Virtual Event Series 2019
      Sponsored By: Horizon Discovery
      2
      SEP 27, 2019 | 9:00 AM
      Investigating how Tpz-1 may be used as a novel therapeutic agent in the treatment of human hematologic cancers
      Investigating how Tpz-1 may be used as a novel therapeutic agent...
      DATE: September 27, 2019TIME: 9:00am PT, 12:00pm ET Thienopyrazole derivatives have recently emerged as effective antitumoral agents with kinase inhibitory activity. In this s...
      Speaker: Jessica Hess
      Sponsored By: Thermo Fisher Scientific/Gibco
      5
      SEP 25, 2019 | 8:00 AM
      Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems:  Case study of Potassium chloride co-transporter KCC2
      Expression, Purification and Characterization of Difficult to...
      DATE: September 25, 2019TIME: 8:00am PT, 11:00am ET This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibcoâ„¢ Expi293â„¢ and ExpiCHOâ„¢ Expres...
      Speaker: Anass Jawhari, PhD
      Sponsored By: Thermo Fisher Scientific/Gibco
      4
      SEP 12, 2019 | 10:30 AM
      Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
      Analysis of human gut microbiomes and antibiotic resistomes of...
      The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
      Speaker: Jonathan Jacobs, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2019
      Sponsored By: QIAGEN
      5
      SEP 10, 2019 | 3:00 PM
      From cell seeding to analysis - Getting the best out of your cell-based assay
      From cell seeding to analysis - Getting the best out of your cell-based...
      DATE: September 10, 2019 TIME: 3:00pm CEST, 06:00am PT, 09:00am ET The assessment of cell health and cellular responses after experimental manipulation continue to be...
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf, Promega
      16
      SEP 05, 2019 | 7:00 AM
      The bread and butter method of genomics: How to improve your (q)PCR
      The bread and butter method of genomics: How to improve your (q)PCR
      DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
      Speaker: Tim Schommartz, PhD
      Sponsored By: Eppendorf
      17 1
    MAR 05, 2020 | 9:00 AM
    An Update on H. pylori testing with a focus on the Houston Consensus recommendations
    An Update on H. pylori testing with a focus on the Houston Consensus...
    Recommendations for key factors in testing and diagnosis of H. pylori infections to include information on appropriate patients for H.pylori testing, current testing options, post treatment...
    Speaker: David Graham, MD
    8
    FEB 25, 2020 | 10:00 AM
    Avances en la Tecnología del Diferencial de Glóbulos Blancos
    Avances en la Tecnología del Diferencial de Glóbulos Blancos
    DATE: 25 Febrero 2020 TIME: 13:00 COT, 10:00 PST Los desarrollos tecnológicos en analizadores automatizados de Hematología continúan transformando el laboratorio de Hema...
    Speaker: Don Wright
    Sponsored By: Abbott
    5 1
    FEB 05, 2020 | 8:00 AM
    C.E. CREDITS
    Emerging Issues in the Coagulation Laboratory
    Emerging Issues in the Coagulation Laboratory
    Learn how new hemophilia and anticoagulant therapies impact coagulation assays and clinical decisions in our educational session....
    Speaker: Donna Castellone, MS, MASCP, MT(ASCP) SH
    Presented at: Stago EdVantage Virtual University
    Sponsored By: Diagnostica Stago, Inc.
    10 1
    JAN 23, 2020 | 9:00 AM
    A Workflow for Knock-in Genome Editing: Simplified
    A Workflow for Knock-in Genome Editing: Simplified
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    Speaker: Matthew Poling, PhD
    Sponsored By: Thermo Fisher Scientific
    17
    JAN 14, 2020 | 9:00 AM
    Fecal Elastase-1 - a Biomarker for Pancreatic Exocrine Insufficiency
    Fecal Elastase-1 - a Biomarker for Pancreatic Exocrine Insufficiency
    Fecal elastase-1 – a biomarker for pancreatic exocrine insufficiency(EPI) continues to gain traction as an ideal biomarker for assessing EPI. This presentation will include a review of...
    Speaker: Roy Sherwood, BSc, MSC, DPhil
    Sponsored By: Diasorin S.p.A.
    5
     
     
  • JAN 01, 2020 | 6:00 AM
    Microphysiological Systems for Human Focused Drug Discovery
    Microphysiological Systems for Human Focused Drug Discovery
    Microphysiological systems (MPS), also known as organ-on-chips, are small scale in vitro cell cultures which mimic facets of tissue or organ level function. MPS frequently utilise primary hu...
    Speaker: Tomasz Kostrzewski, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2020
    3
    JAN 01, 2020 | 6:00 AM
    Generation of expanded primary liver cells
    Generation of expanded primary liver cells
    The use of primary cells in vitro is compromised by the limited quantity of cells that can be isolated from one donor, a lack of or very restricted proliferation capacity (e.g. hepatocytes)...
    Speaker: Astrid Nörenberg, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2020
    1
    JAN 01, 2020 | 6:00 AM
    Organoids: A Revolutionary New Tool for Drug Screening
    Organoids: A Revolutionary New Tool for Drug Screening
    “The poor translatability of early-stage preclinical models is a major setback in oncology drug development. Immortalized cell lines, that are extensively used in drug screens, undergo...
    Speaker: Rekha Pal, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2020
    1
    DEC 17, 2019 | 8:00 AM
    Single-Cell Phosphoprotein Analysis with CyTOF: From Training to Immuno-Oncology Clinical Research
    Single-Cell Phosphoprotein Analysis with CyTOF: From Training...
    DATE: December 17, 2019TIME: 8:00am PSTMass cytometry instruments simultaneously measure >50 cellular features, with a typical experiment including millions of cells. Dysregulated...
    Speaker: Jonathan Irish, PhD , Caroline E. Roe, BS
    Sponsored By: Standard BioTools
    7 2
    DEC 11, 2019 | 7:00 AM
    A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
    A Sensitive and Robust Plasmid Analysis Method by Capillary Electrophoresis...
    DATE: December 11, 2019TIME: 7:00am PST, 10:00am EST An important part of the biopharma industry, Plasmid DNA is used as a gene-delivery vehicle for DNA vaccination and as a key...
    Speaker: Colleen Reed
    Sponsored By: SCIEX
    7
     
     
  • DEC 10, 2019 | 8:00 AM
    Expi293: New Tools for Structural Biology and Difficult to Express Proteins
    Expi293: New Tools for Structural Biology and Difficult to Express...
    DATE: December 10, 2019TIME: 8:00am PST, 11:00am EST In this presentation, we demonstrate the performance of a new range of Expi293 components, including engineered Expi293 cell...
    Speaker: Jon Zmuda, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    7 1
    DEC 04, 2019 | 7:00 AM
    Improve your reproducibility - Liquid Handling is a crucial factor Exemplarily shown for NGS, ELISA and qPCR
    Improve your reproducibility - Liquid Handling is a crucial factor...
    DATE: December 4, 2019TIME: 7:00am PST, 10:00am EST, 4:00pm CET Do you know how it feels when you just quickly want to redo an experiment that your colleague did, or an experim...
    Speaker: Katja Baesler, PhD , Ulrike Gast, PhD , Alisa Gruschka
    Sponsored By: Eppendorf
    12
    NOV 15, 2019 | 8:00 AM
    Exosomes and super-resolution microscopy
    Exosomes and super-resolution microscopy
    DATE: November 15, 2019TIME: 8:00am PST, 11:00am EST Exosomes are small vesicles, ranging from 30-120 nanometers in diameter, secreted from cells throughout the human body. The...
    Speaker: Ryan McNamara, PhD
    Sponsored By: ONI
    8
    NOV 14, 2019 | 6:00 AM
    High Resolution View of D4Z4 Repeat Regions for Studying Facioscapulohumeral Muscular Dystrophy (FSHD) Using Whole Genome Optical Mapping
    High Resolution View of D4Z4 Repeat Regions for Studying Facioscapulohumeral...
    Facioscapulohumeral muscular dystrophy (FSHD), one of the most common forms of progressive muscular dystrophies and is often conventionally diagnosed by Southern blot analysis. The accurate d...
    Speaker: Alka Chaubey, PhD, FACMG
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2019
    Sponsored By: Bionano
    2
    NOV 12, 2019 | 10:00 AM
    Leveraging single cell copy number to resolve tumor clonality
    Leveraging single cell copy number to resolve tumor clonality
    DATE: November 12, 2019TIME: 10:00am PSTDirect measurement of copy number by droplet-based shallow sequencing of genomic DNA has the potential to provide new insights into tumor heterog...
    Speaker: Dr. David Craig
    Sponsored By: 10x Genomics
    9 1
     
     
  • OCT 31, 2019 | 9:00 AM
    Investigation of Endothelial Cell Viability and Growth on 3D printed GelMa Vascular Networks
    Investigation of Endothelial Cell Viability and Growth on 3D printed...
    DATE: October 31, 2019TIME: 9:00am PDT, 12:00pm EDT INTRODUCTION: A major limitation for the development of 3D engineered tissues is the absence of viable and perfusable...
    Speaker: Chrisoula Chappell
    Sponsored By: Thermo Fisher Scientific/Gibco
    8
    OCT 22, 2019 | 9:00 AM
    Preserving Data Integrity: 21 CFR Part 11 Compliance and Osmolality as a Process Parameter
    Preserving Data Integrity: 21 CFR Part 11 Compliance and Osmolality...
    DATE: October 22, 2019 TIME: 9:00am PDT, 12:00PM EDT...
    Speaker: Angela Bazigos , Kristeena Wright, PhD , Shweta Nair
    Sponsored By: Advanced Instruments
    10 1
    OCT 10, 2019 | 6:00 AM
    Highly-efficient and -specific genome editing with Cas9 nickase ribonucleoproteins in Primary T-cells
    Highly-efficient and -specific genome editing with Cas9 nickase...
    In this webinar, we will be discussing some of our most recent testing using our Cas9 proteins, Cas9 RNP nickases in a variety of human cell types, including primary T-cells. Cas nucleases wh...
    Speaker: Jacob Lamberth
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2019
    Sponsored By: MilliporeSigma
    1
    OCT 03, 2019 | 12:00 PM
    Gene Editing Success: Considerations for maximizing your CRISPR gene editing accuracy and efficiency
    Gene Editing Success: Considerations for maximizing your CRISPR...
    CRISPR based gene editing has proven to be an incredibly powerful tool for studying functions of individual proteins, understanding the role of multi-gene pathways, and even knocking out prot...
    Speaker: Ryan Donnelly
    Presented at: CRISPR Virtual Event Series 2019
    Sponsored By: Horizon Discovery
    2
    SEP 27, 2019 | 9:00 AM
    Investigating how Tpz-1 may be used as a novel therapeutic agent in the treatment of human hematologic cancers
    Investigating how Tpz-1 may be used as a novel therapeutic agent...
    DATE: September 27, 2019TIME: 9:00am PT, 12:00pm ET Thienopyrazole derivatives have recently emerged as effective antitumoral agents with kinase inhibitory activity. In this s...
    Speaker: Jessica Hess
    Sponsored By: Thermo Fisher Scientific/Gibco
    5
     
     
  • SEP 25, 2019 | 8:00 AM
    Expression, Purification and Characterization of Difficult to Express Membrane Proteins in the Expi293 and ExpiCHO Expression Systems:  Case study of Potassium chloride co-transporter KCC2
    Expression, Purification and Characterization of Difficult to...
    DATE: September 25, 2019TIME: 8:00am PT, 11:00am ET This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibcoâ„¢ Expi293â„¢ and ExpiCHOâ„¢ Expres...
    Speaker: Anass Jawhari, PhD
    Sponsored By: Thermo Fisher Scientific/Gibco
    4
    SEP 12, 2019 | 10:30 AM
    Analysis of human gut microbiomes and antibiotic resistomes of pediatric patients undergoing stem cell transplantation
    Analysis of human gut microbiomes and antibiotic resistomes of...
    The emergence and spread of antimicrobial-resistant (AMR) bacteria and resistance mechanisms pose a serious challenge to laboratories in both hospital clinical microbiology and public health...
    Speaker: Jonathan Jacobs, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2019
    Sponsored By: QIAGEN
    5
    SEP 10, 2019 | 3:00 PM
    From cell seeding to analysis - Getting the best out of your cell-based assay
    From cell seeding to analysis - Getting the best out of your cell-based...
    DATE: September 10, 2019 TIME: 3:00pm CEST, 06:00am PT, 09:00am ET The assessment of cell health and cellular responses after experimental manipulation continue to be...
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf, Promega
    16
    SEP 05, 2019 | 7:00 AM
    The bread and butter method of genomics: How to improve your (q)PCR
    The bread and butter method of genomics: How to improve your (q)PCR
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    Speaker: Tim Schommartz, PhD
    Sponsored By: Eppendorf
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