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    Next-Generation Sequencing

    Next-Generation Sequencing: A high-throughput method used to determine a portion of the nucleotide sequence of an individual's genome. This technique utilizes DNA sequencing technologies that are capable of processing multiple DNA sequences in parallel. Also called massively parallel sequencing and NGS.

    Webinars

    • OCT 03, 2018 | 10:30 AM
      Integrating Novel Advances in Gene Delivery and Genome Engineering for Therapeutic Application
      Integrating Novel Advances in Gene Delivery and Genome Engineering...
      There have been an increasing number of successful human gene therapy clinical trials, and in particular gene delivery vehicles or vectors based on the adeno-associated virus (AAV) have enabl...
      Speaker: David Schaffer, PhD
      Presented at: CRISPR Virtual Event Series 2018
      2
      OCT 03, 2018 | 9:00 AM
      Design, Edit, Analyze and Maximize Your CRISPR-Cas9 Editing Efficiency
      Design, Edit, Analyze and Maximize Your CRISPR-Cas9 Editing Efficiency
      Learn how to optimize CRISPR-Cas9 editing efficiencies in cell lines and primary cell types using modified synthetic sgRNA. Includes tips for achieving maximum knockout and knock-in efficienc...
      Speaker: Kevin Holden, PhD
      Presented at: CRISPR Virtual Event Series 2018
      Sponsored By: Synthego
      2
      OCT 03, 2018 | 9:00 AM
      CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs in CRISPR-Cas9 Genome-Editing Systems
      CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs...
      The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...
      Speaker: Lihua Julie Zhu, PhD
      Presented at: CRISPR Virtual Event Series 2018
      3
      OCT 03, 2018 | 7:30 AM
      Keynote Presentation: Measurements and Standards to Support Confidence in Genome Editing
      Keynote Presentation: Measurements and Standards to Support Confidence...
      The National Institute of Standards and Technology (NIST), is the measurement institute of the U.S., supplying unbiased expertise in measurements and standards to support growth in all sector...
      Speaker: Samantha Maragh, PhD
      Presented at: CRISPR Virtual Event Series 2018
      2
      OCT 03, 2018 | 6:00 AM
      Identification of Pre-existing Adaptive Immune Responses to Cas9 in Humans
      Identification of Pre-existing Adaptive Immune Responses to Cas9...
      The CRISPR-Cas9 system has proven to be a powerful tool for genome editing allowing for the precise modification of specific DNA sequences within a cell. Many efforts are currently underway t...
      Speaker: Carsten Charlesworth, PhD
      Presented at: CRISPR Virtual Event Series 2018
      2
      SEP 27, 2018 | 8:00 AM
      Achieving higher throughput with multiplexed phage display
      Achieving higher throughput with multiplexed phage display
      DATE: September 27, 2018TIME: 8:00AM PDTRecent advances in phage display for molecular evolution incorporate deep sequencing to enable greater library coverage. However, severe gap...
      Speaker: Thomas Hengstl, PhD
      Sponsored By: ForteBio LLC
      6
      SEP 18, 2018 | 8:00 AM
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
      DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
      Speaker: Judd Hultquist, PhD
      Sponsored By: Horizon Discovery
      7
      SEP 18, 2018 | 7:00 AM
      Scaling end-to-end NGS solutions for clinical diagnostics in the cloud
      Scaling end-to-end NGS solutions for clinical diagnostics in the...
      DATE: September 18, 2018TIME:  07:00am PDT, 2:00pm GMT Next Generation Sequencing has become an essential tool in clinical diagnostic laboratories, however this technolo...
      Speaker: Joo Wook Ahn , Andrew Bond , Mattias Jansson , Alessandro Riccombeni, PhD
      Sponsored By: DNAnexus, QIAGEN
      6
      SEP 12, 2018 | 8:00 AM
      Advancing precision immunotherapy through next generation sequencing of T-cell receptors: Part II
      Advancing precision immunotherapy through next generation sequencing...
      DATE:  September 12, 2018 TIME:  08:00am PDT, 11:00am EDT In the last presentation of this webinar series, we will continue our discussions on recent advancements i...
      Speaker: Timothy Looney, PhD
      Sponsored By: Thermo Fisher Scientific - Ion Torrent
      9
      AUG 16, 2018 | 8:00 AM
      Advancing precision immunotherapy through next-generation sequencing of T cell receptors: Part I
      Advancing precision immunotherapy through next-generation sequencing...
      DATE: August, 16, 2018TIME: 08:00AM PDTThis webinar will review recent advancements in the application of next-generation sequencing of T cell receptor beta (TCRB) chain repertoires towards...
      Speaker: Timothy Looney, PhD
      Sponsored By: Thermo Fisher Scientific - Ion Torrent
      7
      AUG 09, 2018 | 8:00 AM
      Exon-level array: Bridging the gap between chromosomal microarray and next-gen sequencing
      Exon-level array: Bridging the gap between chromosomal microarray...
      DATE:  August 9, 2018TIME:  8:00am PDT, 11:00am EDT Current exon-level arrays and NGS techniques for CNV detection are plagued with a high number of false-positive...
      Speaker: Nicole de Leeuw, PhD , Kristina Cusmano-Ozog, MD
      Sponsored By: Thermo Fisher Scientific - Applied Biosystems
      9
      AUG 08, 2018 | 1:00 PM
      Deciphering the cancer immunity cycle through next generation sequencing
      Deciphering the cancer immunity cycle through next generation...
      DATE:  August 8, 2018 TIME:  1:00pm PDT, 4:00pm EDT To kick off our three-part immuno-oncology webinar series, Dr. Cawley will highlight recent advancements driven...
      Speaker: Simon Cawley, PhD
      Sponsored By: Thermo Fisher Scientific - Ion Torrent
      8 2
      AUG 07, 2018 | 8:00 AM
      Using Reference Standards to Validate your NGS Oncology Workflow
      Using Reference Standards to Validate your NGS Oncology Workflow
      DATE: August 7, 2018TIME: 08:00AM PDT, 11:00AM EDTWe are currently at an exciting precipice in clinical oncology management, where an increased rate of biomarker identification is drivi...
      Speaker: Hannah Child, PhD
      Sponsored By: Horizon Discovery
      10 1
      JUN 21, 2018 | 12:00 PM
      Whole Genome Sequencing As A Valuable Clinical Tool For the Management of Cancer Patients
      Whole Genome Sequencing As A Valuable Clinical Tool For the Management...
      Advances in DNA sequencing, based upon massively parallel sequencing, has resulted in dramatic advances in DNA sequence output in the past few years. It is now possible to generate terrabases...
      Speaker: David Smith, PhD
      Presented at: Precision Medicine Virtual Event Series 2018
      1
      JUN 21, 2018 | 10:30 AM
      Setting Up Next-Generation Sequencing for the Needs of Routine Molecular Diagnostics With a Focus on the Suitability of the GeneReader NGS System for Lung Cancer Patients
      Setting Up Next-Generation Sequencing for the Needs of Routine...
      The introduction of targeted therapies into the clinical management of lung adenocarcinoma has had a massive impact on patient care. Multiple driver mutations are now druggable and treatments...
      Speaker: Paul Hofman, MD, PhD
      Presented at: Precision Medicine Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      JUN 20, 2018 | 6:00 AM
      Standardized Precision Medicine? - A Quality Management Perspective on NGS-based Individualized Therapies
      Standardized Precision Medicine? - A Quality Management Perspective...
      Precision medicine and next-generation sequencing – Two terms characterized by a highly dynamic nature, strong innovations and a bright future. Both merged together will exert a decisiv...
      Speaker: Christoph Endrullat, MS
      Presented at: Precision Medicine Virtual Event Series 2018
      2
      MAY 10, 2018 | 10:30 AM
      C.E. CREDITS
      Strain Level Microbiome Profiling Using rRNA Gene Amplicons
      Strain Level Microbiome Profiling Using rRNA Gene Amplicons
      An ideal microbiome profiling method would produce strain level taxonomic classification of organisms and their relative quantities. We demonstrate proof of principle experiments that sequenc...
      Speaker: Mark Driscoll, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      1
      MAY 09, 2018 | 1:30 PM
      Next-Generation Sequencing to Understand the Function of miRNAs in Human IPSC-Cardiomyocyte Disease Models
      Next-Generation Sequencing to Understand the Function of miRNAs...
      There is increasing evidence that intracellular miRNAs play a role in the pathogenesis of many complex disease phenotypes.  In addition, extracellular miRNAs in exosomes are emerging as...
      Speaker: Ulrich Broeckel, MD , Amy Turner, BS
      Presented at: Genetics and Genomics Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      MAY 09, 2018 | 12:00 PM
      Using Genome Wide Sequencing to Identify a Gene-Gene Interaction
      Using Genome Wide Sequencing to Identify a Gene-Gene Interaction
      In this presentation I will describe results from a family study designed to identify the genetic cause for familial clustering of several early onset cancers.   Data from next generatio...
      Speaker: Christopher I Amos, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      1
      MAY 09, 2018 | 9:00 AM
      Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
      Gene Expression Analysis - Critical Factors for Overcoming Challenges...
      Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
      Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2018
      Sponsored By: QIAGEN
      1
      MAY 03, 2018 | 11:00 AM
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
      Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
      DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
      Speaker: Sisi Li
      Sponsored By: Covaris
      5 2
      APR 17, 2018 | 8:00 AM
      StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based Neural Spheroid Platform for  High Throughput Drug Screening
      StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based...
      DATE: April 17, 2018TIME: 8:00AM PST, 11:00AM ESTRecently, much research has focused on obtaining 3D brain organoids in an attempt to better recapitulate brain development and function...
      Speaker: Cassiano Carromeu, PhD , Grischa Chandy, PhD , Oksana Sirenko, PhD
      Sponsored By: Molecular Devices
      7 1
      APR 12, 2018 | 12:00 PM
      Standards for Mixed Pathogen Detection via Metagenomics
      Standards for Mixed Pathogen Detection via Metagenomics
      I will update the current state of our candidate RM 8376 for mixed microbial DNA detection via NGS-based sequencing and metagenomics. After community engagement to understand the metrological...
      Speaker: Jason Kralj, PhD
      Presented at: Molecular Diagnostics Virtual Event Series 2018
      1
      APR 11, 2018 | 12:00 PM
      Emerging Trends: Cell Free (cf)DNA-Based Screening for Common Aneuploidies
      Emerging Trends: Cell Free (cf)DNA-Based Screening for Common...
      Screening for Down syndrome began with the question "How old will you be when you deliver?".  If the pregnant woman answered 35 years of age or older, she was offered amniocent...
      Speaker: Glenn Palomaki, PhD
      Presented at: Molecular Diagnostics Virtual Event Series 2018
      1
    OCT 03, 2018 | 10:30 AM
    Integrating Novel Advances in Gene Delivery and Genome Engineering for Therapeutic Application
    Integrating Novel Advances in Gene Delivery and Genome Engineering...
    There have been an increasing number of successful human gene therapy clinical trials, and in particular gene delivery vehicles or vectors based on the adeno-associated virus (AAV) have enabl...
    Speaker: David Schaffer, PhD
    Presented at: CRISPR Virtual Event Series 2018
    2
    OCT 03, 2018 | 9:00 AM
    Design, Edit, Analyze and Maximize Your CRISPR-Cas9 Editing Efficiency
    Design, Edit, Analyze and Maximize Your CRISPR-Cas9 Editing Efficiency
    Learn how to optimize CRISPR-Cas9 editing efficiencies in cell lines and primary cell types using modified synthetic sgRNA. Includes tips for achieving maximum knockout and knock-in efficienc...
    Speaker: Kevin Holden, PhD
    Presented at: CRISPR Virtual Event Series 2018
    Sponsored By: Synthego
    2
    OCT 03, 2018 | 9:00 AM
    CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs in CRISPR-Cas9 Genome-Editing Systems
    CRISPRseek and GUIDEseq for Design of Target-Specific Guide RNAs...
    The most recently developed genome editing system, CRISPR-Cas9 has greater inherent flexibility than prior programmable nuclease platforms. Because of its simplicity and efficacy, this techno...
    Speaker: Lihua Julie Zhu, PhD
    Presented at: CRISPR Virtual Event Series 2018
    3
    OCT 03, 2018 | 7:30 AM
    Keynote Presentation: Measurements and Standards to Support Confidence in Genome Editing
    Keynote Presentation: Measurements and Standards to Support Confidence...
    The National Institute of Standards and Technology (NIST), is the measurement institute of the U.S., supplying unbiased expertise in measurements and standards to support growth in all sector...
    Speaker: Samantha Maragh, PhD
    Presented at: CRISPR Virtual Event Series 2018
    2
    OCT 03, 2018 | 6:00 AM
    Identification of Pre-existing Adaptive Immune Responses to Cas9 in Humans
    Identification of Pre-existing Adaptive Immune Responses to Cas9...
    The CRISPR-Cas9 system has proven to be a powerful tool for genome editing allowing for the precise modification of specific DNA sequences within a cell. Many efforts are currently underway t...
    Speaker: Carsten Charlesworth, PhD
    Presented at: CRISPR Virtual Event Series 2018
    2
     
     
  • SEP 27, 2018 | 8:00 AM
    Achieving higher throughput with multiplexed phage display
    Achieving higher throughput with multiplexed phage display
    DATE: September 27, 2018TIME: 8:00AM PDTRecent advances in phage display for molecular evolution incorporate deep sequencing to enable greater library coverage. However, severe gap...
    Speaker: Thomas Hengstl, PhD
    Sponsored By: ForteBio LLC
    6
    SEP 18, 2018 | 8:00 AM
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    High-throughput CRISPR-Cas9 Genome Engineering in Primary T Cells
    DATE: September 18,2018TIME: 08:00am PDT, 11:00am EDT CRISPR-Cas9 gene editing strategies have revolutionized our ability to engineer the genomes of diverse cell types and sp...
    Speaker: Judd Hultquist, PhD
    Sponsored By: Horizon Discovery
    7
    SEP 18, 2018 | 7:00 AM
    Scaling end-to-end NGS solutions for clinical diagnostics in the cloud
    Scaling end-to-end NGS solutions for clinical diagnostics in the...
    DATE: September 18, 2018TIME:  07:00am PDT, 2:00pm GMT Next Generation Sequencing has become an essential tool in clinical diagnostic laboratories, however this technolo...
    Speaker: Joo Wook Ahn , Andrew Bond , Mattias Jansson , Alessandro Riccombeni, PhD
    Sponsored By: DNAnexus, QIAGEN
    6
    SEP 12, 2018 | 8:00 AM
    Advancing precision immunotherapy through next generation sequencing of T-cell receptors: Part II
    Advancing precision immunotherapy through next generation sequencing...
    DATE:  September 12, 2018 TIME:  08:00am PDT, 11:00am EDT In the last presentation of this webinar series, we will continue our discussions on recent advancements i...
    Speaker: Timothy Looney, PhD
    Sponsored By: Thermo Fisher Scientific - Ion Torrent
    9
    AUG 16, 2018 | 8:00 AM
    Advancing precision immunotherapy through next-generation sequencing of T cell receptors: Part I
    Advancing precision immunotherapy through next-generation sequencing...
    DATE: August, 16, 2018TIME: 08:00AM PDTThis webinar will review recent advancements in the application of next-generation sequencing of T cell receptor beta (TCRB) chain repertoires towards...
    Speaker: Timothy Looney, PhD
    Sponsored By: Thermo Fisher Scientific - Ion Torrent
    7
     
     
  • AUG 09, 2018 | 8:00 AM
    Exon-level array: Bridging the gap between chromosomal microarray and next-gen sequencing
    Exon-level array: Bridging the gap between chromosomal microarray...
    DATE:  August 9, 2018TIME:  8:00am PDT, 11:00am EDT Current exon-level arrays and NGS techniques for CNV detection are plagued with a high number of false-positive...
    Speaker: Nicole de Leeuw, PhD , Kristina Cusmano-Ozog, MD
    Sponsored By: Thermo Fisher Scientific - Applied Biosystems
    9
    AUG 08, 2018 | 1:00 PM
    Deciphering the cancer immunity cycle through next generation sequencing
    Deciphering the cancer immunity cycle through next generation...
    DATE:  August 8, 2018 TIME:  1:00pm PDT, 4:00pm EDT To kick off our three-part immuno-oncology webinar series, Dr. Cawley will highlight recent advancements driven...
    Speaker: Simon Cawley, PhD
    Sponsored By: Thermo Fisher Scientific - Ion Torrent
    8 2
    AUG 07, 2018 | 8:00 AM
    Using Reference Standards to Validate your NGS Oncology Workflow
    Using Reference Standards to Validate your NGS Oncology Workflow
    DATE: August 7, 2018TIME: 08:00AM PDT, 11:00AM EDTWe are currently at an exciting precipice in clinical oncology management, where an increased rate of biomarker identification is drivi...
    Speaker: Hannah Child, PhD
    Sponsored By: Horizon Discovery
    10 1
    JUN 21, 2018 | 12:00 PM
    Whole Genome Sequencing As A Valuable Clinical Tool For the Management of Cancer Patients
    Whole Genome Sequencing As A Valuable Clinical Tool For the Management...
    Advances in DNA sequencing, based upon massively parallel sequencing, has resulted in dramatic advances in DNA sequence output in the past few years. It is now possible to generate terrabases...
    Speaker: David Smith, PhD
    Presented at: Precision Medicine Virtual Event Series 2018
    1
    JUN 21, 2018 | 10:30 AM
    Setting Up Next-Generation Sequencing for the Needs of Routine Molecular Diagnostics With a Focus on the Suitability of the GeneReader NGS System for Lung Cancer Patients
    Setting Up Next-Generation Sequencing for the Needs of Routine...
    The introduction of targeted therapies into the clinical management of lung adenocarcinoma has had a massive impact on patient care. Multiple driver mutations are now druggable and treatments...
    Speaker: Paul Hofman, MD, PhD
    Presented at: Precision Medicine Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
     
     
  • JUN 20, 2018 | 6:00 AM
    Standardized Precision Medicine? - A Quality Management Perspective on NGS-based Individualized Therapies
    Standardized Precision Medicine? - A Quality Management Perspective...
    Precision medicine and next-generation sequencing – Two terms characterized by a highly dynamic nature, strong innovations and a bright future. Both merged together will exert a decisiv...
    Speaker: Christoph Endrullat, MS
    Presented at: Precision Medicine Virtual Event Series 2018
    2
    MAY 10, 2018 | 10:30 AM
    C.E. CREDITS
    Strain Level Microbiome Profiling Using rRNA Gene Amplicons
    Strain Level Microbiome Profiling Using rRNA Gene Amplicons
    An ideal microbiome profiling method would produce strain level taxonomic classification of organisms and their relative quantities. We demonstrate proof of principle experiments that sequenc...
    Speaker: Mark Driscoll, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    1
    MAY 09, 2018 | 1:30 PM
    Next-Generation Sequencing to Understand the Function of miRNAs in Human IPSC-Cardiomyocyte Disease Models
    Next-Generation Sequencing to Understand the Function of miRNAs...
    There is increasing evidence that intracellular miRNAs play a role in the pathogenesis of many complex disease phenotypes.  In addition, extracellular miRNAs in exosomes are emerging as...
    Speaker: Ulrich Broeckel, MD , Amy Turner, BS
    Presented at: Genetics and Genomics Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
    MAY 09, 2018 | 12:00 PM
    Using Genome Wide Sequencing to Identify a Gene-Gene Interaction
    Using Genome Wide Sequencing to Identify a Gene-Gene Interaction
    In this presentation I will describe results from a family study designed to identify the genetic cause for familial clustering of several early onset cancers.   Data from next generatio...
    Speaker: Christopher I Amos, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    1
    MAY 09, 2018 | 9:00 AM
    Gene Expression Analysis - Critical Factors for Overcoming Challenges Using qPCR
    Gene Expression Analysis - Critical Factors for Overcoming Challenges...
    Gene expression studies give a snapshot of the different types of RNAs being expressed at a given time point in cells or tissues in vivo. Different RNA types have different roles, for example...
    Speaker: Ina Scheuerpflug, PhD , Verena Schramm, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2018
    Sponsored By: QIAGEN
    1
     
     
  • MAY 03, 2018 | 11:00 AM
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue - An Investigation of How Stress Alters 5hmC by Mediating Transcription Factor (TF) Binding
    Performing ChIP Using Low Amounts of Frozen Mouse Brain Tissue...
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    Speaker: Sisi Li
    Sponsored By: Covaris
    5 2
    APR 17, 2018 | 8:00 AM
    StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based Neural Spheroid Platform for  High Throughput Drug Screening
    StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based...
    DATE: April 17, 2018TIME: 8:00AM PST, 11:00AM ESTRecently, much research has focused on obtaining 3D brain organoids in an attempt to better recapitulate brain development and function...
    Speaker: Cassiano Carromeu, PhD , Grischa Chandy, PhD , Oksana Sirenko, PhD
    Sponsored By: Molecular Devices
    7 1
    APR 12, 2018 | 12:00 PM
    Standards for Mixed Pathogen Detection via Metagenomics
    Standards for Mixed Pathogen Detection via Metagenomics
    I will update the current state of our candidate RM 8376 for mixed microbial DNA detection via NGS-based sequencing and metagenomics. After community engagement to understand the metrological...
    Speaker: Jason Kralj, PhD
    Presented at: Molecular Diagnostics Virtual Event Series 2018
    1
    APR 11, 2018 | 12:00 PM
    Emerging Trends: Cell Free (cf)DNA-Based Screening for Common Aneuploidies
    Emerging Trends: Cell Free (cf)DNA-Based Screening for Common...
    Screening for Down syndrome began with the question "How old will you be when you deliver?".  If the pregnant woman answered 35 years of age or older, she was offered amniocent...
    Speaker: Glenn Palomaki, PhD
    Presented at: Molecular Diagnostics Virtual Event Series 2018
    1
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