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    Molecular Pathology

    Molecular Pathology: is an emerging discipline within pathology that is focused on the study and diagnosis of disease through the examination of molecules within organs, tissues or bodily fluids. Molecular pathology shares some aspects of practice with both anatomic pathology and clinical pathology, molecular biology, biochemistry, proteomics and genetics, and is sometimes considered a "crossover" discipline. It is multi-disciplinary in nature and focuses mainly on the sub-microscopic aspects of disease.

    Webinars

    • NOV 09, 2017 | 10:30 AM
      Fast Phenotypic Antibiotic Susceptibility Testing of Positive Blood Cultures
      Fast Phenotypic Antibiotic Susceptibility Testing of Positive...
      The role of the microbiology laboratory in the processing of positive patient blood cultures has become more complicated with the increasing demand for rapid information to assist in the mana...
      Speaker: Margie Morgan, PhD, D(ABMM), MT(ASCP)
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
      Sponsored By: Accelerate Diagnostics
      3
      NOV 09, 2017 | 9:00 AM
      Monitoring Pain Management Medications in Pregnancy and the Neonate
      Monitoring Pain Management Medications in Pregnancy and the Neonate
      Acute and chronic pain are often managed with drug therapy, which may include extended use of drug classes such as opioids and benzodiazepines.  Pain does not go away, and may intensify...
      Speaker: Gwen McMillin, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
      1
      NOV 09, 2017 | 7:30 AM
      Keynote Presentation: Integration of the Simplexa C. difficile Direct assay into a two-step algorithm for the laboratory diagnosis of C. difficile
      Keynote Presentation: Integration of the Simplexa C. difficile...
      Clostridium difficile infection (CDI) has become the leading cause of healthcare associated infections in the United States and currently accounts for 15% to 20% of infectious diarrhea in the...
      Speaker: Dr. James Snyder
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
      1
      NOV 08, 2017 | 6:00 PM
      Utilization of Stem Cells to Crack Motor Neuron Development and Degeneration
      Utilization of Stem Cells to Crack Motor Neuron Development and...
      Regulatory non coding RNAs, including microRNAs (miRNAs) and long non coding RNAs (lncRNAs), have shown to be essential for animal development and viability, yet dissecting the relevance of i...
      Speaker: Jun-An Chen
      Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
      5 1
      NOV 08, 2017 | 12:00 PM
      Combined immunohistochemistry and RNA in situ hybridization to elucidate tumor molecular heterogeneity in prostate cancer
      Combined immunohistochemistry and RNA in situ hybridization to...
      Currently, prostate cancer is the second leading cause of death from cancer in North America, the most frequent malignancy in men from all ethnicities, surpassing lung cancer. Prostate tumors...
      Speaker: Nallasivam Palanisamy, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
      Sponsored By: Advanced Cell Diagnostics
      3
      NOV 08, 2017 | 10:30 AM
      Liquid Chromatography Tandem Mass Spectrometry Research into Testosterone Analysis: Considerations of Instrument Performance Versus Sample Preparation Complexity
      Liquid Chromatography Tandem Mass Spectrometry Research into Testosterone...
      Many direct immunoassays in use today for testosterone analysis are little better than a guess at the low levels that are sometimes required.  This has led many clinical societies to adv...
      Speaker: Joshua Hayden, PhD, DABCC, FACB
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
      Sponsored By: Agilent
      2
      NOV 08, 2017 | 6:00 AM
      How Does the ABIM Choosing Wisely Initiative Affect Diagnostic Laboratory Medicine?
      How Does the ABIM Choosing Wisely Initiative Affect Diagnostic...
      In 2012, Consumers Reports and the American Board of Internal Medicine Foundation launched the Choosing Wisely campaign. This initiative, which attracts media attention, assists physicians, n...
      Speaker: George Fritsma, MS MLS
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
      3
      NOV 07, 2017 | 8:00 AM
      WEBINAR: The power of multiplexing and applications of the QuantiGene Plex Assay in oncology research and diagnostics
      WEBINAR: The power of multiplexing and applications of the QuantiGene...
      Cancer research diagnostic services in pathology laboratories worldwide have been negatively impacted by a lack of fresh-frozen tissue samples, the degradation of DNA and RNA in archival tiss...
      Speaker: Godfrey Grech, PhD
      Presented at: Protein and Cell Analysis Education Series
      Sponsored By: Thermo Fisher Scientific
      2 1
      NOV 02, 2017 | 10:00 AM
      WEBINAR: A CRISPR Way of Making Mice
      WEBINAR: A CRISPR Way of Making Mice
      DATE: November 2, 2017TIME: 10:00am PDT, 1:00pm EDTA CRISPR Way of Making MiceThe bulk of genetic variation associated with human disease exists in the noncoding genome, much of whi...
      Speaker: Joe Miano, PhD
      Sponsored By: Synthego
      12 1
      OCT 25, 2017 | 10:00 AM
      WEBINAR: Antinuclear antibody screening - Making an informed decision
      WEBINAR: Antinuclear antibody screening - Making an informed decision
      DATE: October 25, 2017TIME: 10:00am PDT, 1:00pm EDTTesting for antinuclear antibodies (ANAs) has been a routine part of the evaluation of patients with a suspected connective tissue dis...
      Speaker: Gyorgy Abel, MD, PhD, DABCC , Melissa Snyder, PhD, DABCC
      Sponsored By: Bio-Rad Laboratories
      17 2
      OCT 19, 2017 | 7:00 AM
      WEBINAR: #2: Extended RAS Research Panel on Applied Biosystem's new SeqStudio Genetic Analyzer
      WEBINAR: #2: Extended RAS Research Panel on Applied Biosystem's...
      DATE: October 19, 2017TIME: 7:00 AM PDT, 10:00 AM EDT, 4:00 PM CETThis is the second topic in the webinar series on ‘Sanger Sequencing & Fragment Analysis made easy’...
      Speaker: Dr. Luca Quagliata
      Sponsored By: Thermo Fisher Scientific
      7
      OCT 12, 2017 | 9:00 AM
      Circulating Tumor Cells: Clinical Utility, Challenges, and Future Prospects
      Circulating Tumor Cells: Clinical Utility, Challenges, and Future...
      The detection of CTCs has been used as useful biomarkers in prognosis and monitoring therapeutic response of patients with metastatic cancer. However, during the course of cancer therapy, CTC...
      Speaker: Qing Meng, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
      2
      OCT 11, 2017 | 9:00 AM
      Personalizing Melanoma Management with Molecular Diagnostics
      Personalizing Melanoma Management with Molecular Diagnostics
      More and more decisions will center on the ability to molecularly characterize a disease. So the molecular diagnostic laboratory will have a pivotal role. What does that look like? Will we be...
      Speaker: Federico Monzon, MD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
      1
      SEP 27, 2017 | 9:00 AM
      WEBINAR: Challenges in CMV Management
      WEBINAR: Challenges in CMV Management
      DATE:  September 27, 2017TIME:   9:00am PT, 12:00pm ETTransplant Educational Webinar SeriesChallenges in CMV Management Management of CMV infected and immu...
      Speaker: David Hillyard
      Sponsored By: Abbott Molecular Diagnostic, Abbott Molecular Diagnostic
      8
      SEP 14, 2017 | 1:30 PM
      Dissecting the ecological and molecular mechanisms underlying the interaction between plant viruses and their insect vectors
      Dissecting the ecological and molecular mechanisms underlying...
      Plant viruses cause significant damage in terms of reduction in quality and quantity of yield in a wide range of crop plants worldwide. The majority of plant viruses are transmitted from one...
      Speaker: Punya Nachappa, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      2
      SEP 14, 2017 | 12:00 PM
      How to deploy a portable lab for the surveillance of emerging infectious diseases
      How to deploy a portable lab for the surveillance of emerging...
      Genome sequencing can be used to discover new pathogens but it can also be used to monitor known ones. This is known as genomic surveillance and it becoming an increasingly important tool in...
      Speaker: Josh Quick
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      Sponsored By: Oxford Nanopore Technologies
      1
      SEP 14, 2017 | 10:30 AM
      Sex as a Biological Variable in Microbial Pathogenesis
      Sex as a Biological Variable in Microbial Pathogenesis
      Males and females differ in their immunological responses to viral and vaccine antigens, with females typically mounting higher immune responses than males. These sex-based immunological diff...
      Speaker: Sabra L. Klein, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      4
      SEP 14, 2017 | 7:30 AM
      Keynote Presentation: A Mechanistic Approach to Overcoming Antibacterial Drug Resistance
      Keynote Presentation: A Mechanistic Approach to Overcoming Antibacterial...
      Quinolones are one the most commonly prescribed classes of antibacterials in the world and are used to treat a broad variety of Gram-negative and Gram-positive bacterial infections in humans....
      Speaker: Neil Osheroff, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      1
      SEP 14, 2017 | 6:00 AM
      A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: taxonomic and evolutionary considerations
      A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in...
      A rapidly growing number of viruses of lower eukaryotes have been reported in the past few decades. These have enhanced our understanding of virus evolution and diversity. Simultaneously, som...
      Speaker: Nobuhiro Suzuki, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      3
      SEP 13, 2017 | 1:30 PM
      Integration of the Simplexa C. difficile Direct assay in a two-step algorithm for the laboratory diagnosis of C. difficile
      Integration of the Simplexa C. difficile Direct assay in a two-step...
      Clostridium difficile is a major pathogen responsible for infectious diarrhea in healthcare settings. This presentation will review the data from a study comparing the new Simplexa® C. di...
      Speaker: Dr. James Snyder
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      Sponsored By: Diasorin
      2
      SEP 13, 2017 | 12:00 PM
      High Resolution Outbreak Tracing and Resistance Detection using Whole Genome Sequencing in the case of a Mycobacterium tuberculosis Outbreak
      High Resolution Outbreak Tracing and Resistance Detection using...
      Background: In March 2014, a molecular cluster of five multidrug resistant Mycobacterium tuberculosis was detected by the Austrian National Reference Laboratory. An investigation was initiate...
      Speaker: Winnie Ridderberg, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      1
      SEP 13, 2017 | 9:00 AM
      Insights into virus replication: the role of co-opted host proteins and lipids
      Insights into virus replication: the role of co-opted host proteins...
      Virus – host interactions are currently among the most intensively studied research areas due to the promising new antiviral approaches emerging from these studies. Indeed, RNA viruses,...
      Speaker: Peter L. Nagy, MD., PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      3
      SEP 13, 2017 | 9:00 AM
      How Biomarker Testing Shapes Immunotherapy
      How Biomarker Testing Shapes Immunotherapy
      The immune system is complex and dynamic, focused on defending the body from a host of pathogens ranging from viruses to cancer. A number of different mechanisms have evolved that help the bo...
      Speaker: Condie Carmack, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      Sponsored By: Vela Diagnostics
      1
      SEP 13, 2017 | 7:30 AM
      Keynote Presentation: Using phage to select for evolution of reduced virulence in pathogenic bacteria
      Keynote Presentation: Using phage to select for evolution of reduced...
      Increasing prevalence and severity of multi-drug-resistant (MDR) bacterial infections has necessitated novel antibacterial strategies.  Ideally, new approaches would target bacterial pat...
      Speaker: Paul Turner, PhD
      Presented at: Microbiology & Immunology Virtual Event Series 2017
      1
    NOV 09, 2017 | 10:30 AM
    Fast Phenotypic Antibiotic Susceptibility Testing of Positive Blood Cultures
    Fast Phenotypic Antibiotic Susceptibility Testing of Positive...
    The role of the microbiology laboratory in the processing of positive patient blood cultures has become more complicated with the increasing demand for rapid information to assist in the mana...
    Speaker: Margie Morgan, PhD, D(ABMM), MT(ASCP)
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
    Sponsored By: Accelerate Diagnostics
    3
    NOV 09, 2017 | 9:00 AM
    Monitoring Pain Management Medications in Pregnancy and the Neonate
    Monitoring Pain Management Medications in Pregnancy and the Neonate
    Acute and chronic pain are often managed with drug therapy, which may include extended use of drug classes such as opioids and benzodiazepines.  Pain does not go away, and may intensify...
    Speaker: Gwen McMillin, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
    1
    NOV 09, 2017 | 7:30 AM
    Keynote Presentation: Integration of the Simplexa C. difficile Direct assay into a two-step algorithm for the laboratory diagnosis of C. difficile
    Keynote Presentation: Integration of the Simplexa C. difficile...
    Clostridium difficile infection (CDI) has become the leading cause of healthcare associated infections in the United States and currently accounts for 15% to 20% of infectious diarrhea in the...
    Speaker: Dr. James Snyder
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
    1
    NOV 08, 2017 | 6:00 PM
    Utilization of Stem Cells to Crack Motor Neuron Development and Degeneration
    Utilization of Stem Cells to Crack Motor Neuron Development and...
    Regulatory non coding RNAs, including microRNAs (miRNAs) and long non coding RNAs (lncRNAs), have shown to be essential for animal development and viability, yet dissecting the relevance of i...
    Speaker: Jun-An Chen
    Presented at: 5th annual 24 Hours of Stem Cellsâ„¢ virtual event
    5 1
    NOV 08, 2017 | 12:00 PM
    Combined immunohistochemistry and RNA in situ hybridization to elucidate tumor molecular heterogeneity in prostate cancer
    Combined immunohistochemistry and RNA in situ hybridization to...
    Currently, prostate cancer is the second leading cause of death from cancer in North America, the most frequent malignancy in men from all ethnicities, surpassing lung cancer. Prostate tumors...
    Speaker: Nallasivam Palanisamy, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
    Sponsored By: Advanced Cell Diagnostics
    3
     
     
  • NOV 08, 2017 | 10:30 AM
    Liquid Chromatography Tandem Mass Spectrometry Research into Testosterone Analysis: Considerations of Instrument Performance Versus Sample Preparation Complexity
    Liquid Chromatography Tandem Mass Spectrometry Research into Testosterone...
    Many direct immunoassays in use today for testosterone analysis are little better than a guess at the low levels that are sometimes required.  This has led many clinical societies to adv...
    Speaker: Joshua Hayden, PhD, DABCC, FACB
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
    Sponsored By: Agilent
    2
    NOV 08, 2017 | 6:00 AM
    How Does the ABIM Choosing Wisely Initiative Affect Diagnostic Laboratory Medicine?
    How Does the ABIM Choosing Wisely Initiative Affect Diagnostic...
    In 2012, Consumers Reports and the American Board of Internal Medicine Foundation launched the Choosing Wisely campaign. This initiative, which attracts media attention, assists physicians, n...
    Speaker: George Fritsma, MS MLS
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2017
    3
    NOV 07, 2017 | 8:00 AM
    WEBINAR: The power of multiplexing and applications of the QuantiGene Plex Assay in oncology research and diagnostics
    WEBINAR: The power of multiplexing and applications of the QuantiGene...
    Cancer research diagnostic services in pathology laboratories worldwide have been negatively impacted by a lack of fresh-frozen tissue samples, the degradation of DNA and RNA in archival tiss...
    Speaker: Godfrey Grech, PhD
    Presented at: Protein and Cell Analysis Education Series
    Sponsored By: Thermo Fisher Scientific
    2 1
    NOV 02, 2017 | 10:00 AM
    WEBINAR: A CRISPR Way of Making Mice
    WEBINAR: A CRISPR Way of Making Mice
    DATE: November 2, 2017TIME: 10:00am PDT, 1:00pm EDTA CRISPR Way of Making MiceThe bulk of genetic variation associated with human disease exists in the noncoding genome, much of whi...
    Speaker: Joe Miano, PhD
    Sponsored By: Synthego
    12 1
    OCT 25, 2017 | 10:00 AM
    WEBINAR: Antinuclear antibody screening - Making an informed decision
    WEBINAR: Antinuclear antibody screening - Making an informed decision
    DATE: October 25, 2017TIME: 10:00am PDT, 1:00pm EDTTesting for antinuclear antibodies (ANAs) has been a routine part of the evaluation of patients with a suspected connective tissue dis...
    Speaker: Gyorgy Abel, MD, PhD, DABCC , Melissa Snyder, PhD, DABCC
    Sponsored By: Bio-Rad Laboratories
    17 2
     
     
  • OCT 19, 2017 | 7:00 AM
    WEBINAR: #2: Extended RAS Research Panel on Applied Biosystem's new SeqStudio Genetic Analyzer
    WEBINAR: #2: Extended RAS Research Panel on Applied Biosystem's...
    DATE: October 19, 2017TIME: 7:00 AM PDT, 10:00 AM EDT, 4:00 PM CETThis is the second topic in the webinar series on ‘Sanger Sequencing & Fragment Analysis made easy’...
    Speaker: Dr. Luca Quagliata
    Sponsored By: Thermo Fisher Scientific
    7
    OCT 12, 2017 | 9:00 AM
    Circulating Tumor Cells: Clinical Utility, Challenges, and Future Prospects
    Circulating Tumor Cells: Clinical Utility, Challenges, and Future...
    The detection of CTCs has been used as useful biomarkers in prognosis and monitoring therapeutic response of patients with metastatic cancer. However, during the course of cancer therapy, CTC...
    Speaker: Qing Meng, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
    2
    OCT 11, 2017 | 9:00 AM
    Personalizing Melanoma Management with Molecular Diagnostics
    Personalizing Melanoma Management with Molecular Diagnostics
    More and more decisions will center on the ability to molecularly characterize a disease. So the molecular diagnostic laboratory will have a pivotal role. What does that look like? Will we be...
    Speaker: Federico Monzon, MD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2017
    1
    SEP 27, 2017 | 9:00 AM
    WEBINAR: Challenges in CMV Management
    WEBINAR: Challenges in CMV Management
    DATE:  September 27, 2017TIME:   9:00am PT, 12:00pm ETTransplant Educational Webinar SeriesChallenges in CMV Management Management of CMV infected and immu...
    Speaker: David Hillyard
    Sponsored By: Abbott Molecular Diagnostic, Abbott Molecular Diagnostic
    8
    SEP 14, 2017 | 1:30 PM
    Dissecting the ecological and molecular mechanisms underlying the interaction between plant viruses and their insect vectors
    Dissecting the ecological and molecular mechanisms underlying...
    Plant viruses cause significant damage in terms of reduction in quality and quantity of yield in a wide range of crop plants worldwide. The majority of plant viruses are transmitted from one...
    Speaker: Punya Nachappa, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    2
     
     
  • SEP 14, 2017 | 12:00 PM
    How to deploy a portable lab for the surveillance of emerging infectious diseases
    How to deploy a portable lab for the surveillance of emerging...
    Genome sequencing can be used to discover new pathogens but it can also be used to monitor known ones. This is known as genomic surveillance and it becoming an increasingly important tool in...
    Speaker: Josh Quick
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    Sponsored By: Oxford Nanopore Technologies
    1
    SEP 14, 2017 | 10:30 AM
    Sex as a Biological Variable in Microbial Pathogenesis
    Sex as a Biological Variable in Microbial Pathogenesis
    Males and females differ in their immunological responses to viral and vaccine antigens, with females typically mounting higher immune responses than males. These sex-based immunological diff...
    Speaker: Sabra L. Klein, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    4
    SEP 14, 2017 | 7:30 AM
    Keynote Presentation: A Mechanistic Approach to Overcoming Antibacterial Drug Resistance
    Keynote Presentation: A Mechanistic Approach to Overcoming Antibacterial...
    Quinolones are one the most commonly prescribed classes of antibacterials in the world and are used to treat a broad variety of Gram-negative and Gram-positive bacterial infections in humans....
    Speaker: Neil Osheroff, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    1
    SEP 14, 2017 | 6:00 AM
    A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: taxonomic and evolutionary considerations
    A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in...
    A rapidly growing number of viruses of lower eukaryotes have been reported in the past few decades. These have enhanced our understanding of virus evolution and diversity. Simultaneously, som...
    Speaker: Nobuhiro Suzuki, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    3
    SEP 13, 2017 | 1:30 PM
    Integration of the Simplexa C. difficile Direct assay in a two-step algorithm for the laboratory diagnosis of C. difficile
    Integration of the Simplexa C. difficile Direct assay in a two-step...
    Clostridium difficile is a major pathogen responsible for infectious diarrhea in healthcare settings. This presentation will review the data from a study comparing the new Simplexa® C. di...
    Speaker: Dr. James Snyder
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    Sponsored By: Diasorin
    2
     
     
  • SEP 13, 2017 | 12:00 PM
    High Resolution Outbreak Tracing and Resistance Detection using Whole Genome Sequencing in the case of a Mycobacterium tuberculosis Outbreak
    High Resolution Outbreak Tracing and Resistance Detection using...
    Background: In March 2014, a molecular cluster of five multidrug resistant Mycobacterium tuberculosis was detected by the Austrian National Reference Laboratory. An investigation was initiate...
    Speaker: Winnie Ridderberg, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    1
    SEP 13, 2017 | 9:00 AM
    Insights into virus replication: the role of co-opted host proteins and lipids
    Insights into virus replication: the role of co-opted host proteins...
    Virus – host interactions are currently among the most intensively studied research areas due to the promising new antiviral approaches emerging from these studies. Indeed, RNA viruses,...
    Speaker: Peter L. Nagy, MD., PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    3
    SEP 13, 2017 | 9:00 AM
    How Biomarker Testing Shapes Immunotherapy
    How Biomarker Testing Shapes Immunotherapy
    The immune system is complex and dynamic, focused on defending the body from a host of pathogens ranging from viruses to cancer. A number of different mechanisms have evolved that help the bo...
    Speaker: Condie Carmack, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    Sponsored By: Vela Diagnostics
    1
    SEP 13, 2017 | 7:30 AM
    Keynote Presentation: Using phage to select for evolution of reduced virulence in pathogenic bacteria
    Keynote Presentation: Using phage to select for evolution of reduced...
    Increasing prevalence and severity of multi-drug-resistant (MDR) bacterial infections has necessitated novel antibacterial strategies.  Ideally, new approaches would target bacterial pat...
    Speaker: Paul Turner, PhD
    Presented at: Microbiology & Immunology Virtual Event Series 2017
    1
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