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    Follow

    Pre-Clinical

    Pre-Clinical: relating to or denoting a stage preceding a clinical stage, in particular.

    Webinars

    • NOV 11, 2015 | 6:00 AM
      A Comprehensive Toxicology Screening Solution Targeted to Individual Needs
      A Comprehensive Toxicology Screening Solution Targeted to Individual...
      Drugs of abuse toxicology testing by immunoassays is widely used clinically, but also known to be inadequate for many clinical indications. In this presentation, the speaker will outline diff...
      Speaker: Ping Wang, PhD, DABCC, FACB
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      3
      NOV 11, 2015 | 6:00 AM
      Next-generation sequencing for BRCA1 and BRCA2 mutation testing
      Next-generation sequencing for BRCA1 and BRCA2 mutation testing
      Cancer is complex, but recent findings are yielding a greater understanding of the disease. The tumor suppressor genes BRCA1 and BRCA2 are implicated in breast, ovarian, prostate, and other c...
      Speaker: Raed Samara, PhD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
      2
      NOV 10, 2015 | 8:00 AM
      WEBINAR: Analysis of Chemical Contaminants in Drinking Water: Two techniques to analyze pesticides and disinfection products in drinking water in less time
      WEBINAR: Analysis of Chemical Contaminants in Drinking Water:...
      Date: Nov 10, 2015 8AM Pacific, 11AM Eastern, 4PM GMT Transporting smaller volumes of a sample, injecting less matrix, examining lower limits of detection and staying ahead of government r...
      Speaker: Jonathan Beck, PhD
      Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
      6 1
      OCT 01, 2015 | 12:00 PM
      Can the World's First Animal Study Software to Emulate Clinical Trial Approaches Improve Clinical Outcome Predictability?
      Can the World's First Animal Study Software to Emulate Clinical...
      The 95% failure rate of anticancer therapeutic being approved after positive Phase III trials suggests that existing pre-clinical approaches and tools are inadequate predictors of clinical ou...
      Speaker: Eric Ibsen
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      3
      OCT 01, 2015 | 12:00 PM
      Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR
      Detection and sequencing of rare mutations using mutation enrichment...
      Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challengin...
      Speaker: Mike Makrigiorgos, PhD, DABMP
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      2
      SEP 30, 2015 | 7:30 AM
      Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
      Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
      We have been developing methods to target drugs specifically to pathologic cells, thereby avoiding collateral toxicity to healthy cells. In the case of cancer, we have exploited up-regulation...
      Speaker: Philip Low, PhD
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
      2
      JUL 22, 2015 | 9:00 AM
      WEBINAR: Investigative in vitro Drug Toxicology with Human iPSC-derived Cardiomyocytes
      WEBINAR: Investigative in vitro Drug Toxicology with Human iPSC-derived...
      DATE: July 22, 2015TIME: 9:00AM PT, 12:00PM ETHuman induced pluripotent stem cells (iPSCs) bring human biology into pre-clinical aspects of drug discovery. iPSC-derived cardiomyocytes have em...
      Speaker: Blake Anson, PhD , Mathew Brock, PhD
      Sponsored By: Cellular Dynamics, Cellular Dynamics
      10 1
      MAY 26, 2015 | 6:00 AM
      Cell-Imaging and Luminescence Based Cytotoxicity Analysis using the EnSight Multimode Plate Reader
      Cell-Imaging and Luminescence Based Cytotoxicity Analysis using...
      Sign up here to watch this Webinar On DemandCytotoxicity remains one of the major causes of drug withdrawal and there is an urgent need for reliable and time-saving assay workflows. Cells exp...
      Speaker: Philip Gribbon , Frauke Haenel, PhD
      Sponsored By: Revvity
      24 1
      APR 30, 2015 | 8:00 AM
      Swimming in Transcriptomics: Using Next-Gen sequencing to study the evolution of neural circuitry and swimming behavior of sea slugs
      Swimming in Transcriptomics: Using Next-Gen sequencing to study...
      Sea slugs (Mollusca, Gastropoda, Nudipleura) provide unique opportunities for studying the evolution of behavior at the levels of single neurons, neural circuits, and their synaptic connectio...
      Speaker: Adriano Senatore, PhD, MSc, BSc
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Genomics, Beckman Coulter Genomics
      9
      MAR 19, 2015 | 7:30 AM
      Keynote - The Environmental Causes of Schizophrenia - Developmental Hazards, Social Defeat, and Drug Abuse
      Keynote - The Environmental Causes of Schizophrenia - Developmental...
      Traditional psychiatric textbooks describe schizophrenia as a clinical enigma of unknown aetiology. However, this is no longer true. We now know a great deal about the risk factors, or contr...
      Speaker: Sir Robin Murray, PhD
      Presented at: Neuroscience Virtual Event Series 2015
      13
      FEB 10, 2015 | 12:00 PM
      Primary HPV Cervical Cancer Screening: Supporting data and guidance updates
      Primary HPV Cervical Cancer Screening: Supporting data and guidance...
      Date: Tuesday, February 10th, 2015Time: 12:00PM PST, 3:00PM ESTFor the last 60 years the primary method of preventing cervical cancer in both the U.S. and Europe has been cervical cytology ...
      Speaker: Julia Engstrom-Melnyk, PhD
      Sponsored By: Roche Molecular Diagnostics, Roche Molecular Diagnostics
      8 5
      FEB 10, 2015 | 9:00 AM
      Primary HPV Cervical Cancer Screening: Supporting data and guidance updates
      Primary HPV Cervical Cancer Screening: Supporting data and guidance...
      Date: Tuesday, February 10th, 2015Time: 9:00AM PST, 12:00PM ESTFor the last 60 years the primary method of preventing cervical cancer in both the U.S. and Europe has been cervical cytology ...
      Speaker: Julia Engstrom-Melnyk, PhD
      Sponsored By: Roche Molecular Diagnostics, Roche Molecular Diagnostics
      10 24
      FEB 05, 2015 | 7:30 AM
      C.E. CREDITS
      Small Animal Anesthesia: A New Method to Help Control Variables
      Small Animal Anesthesia: A New Method to Help Control Variables
      An anesthesia system designed to accommodate the physiological characteristics of small animals, including rats and mice, has the potential to provide great practical value to the life scienc...
      Speaker: Andrew H Ide, BS
      Presented at: Laboratory Animal Sciences Virtual Event Series 2015
      12
      FEB 04, 2015 | 1:30 PM
      C.E. CREDITS
      The Surgeons Toolbox: outcomes are only as good as the contents of the box
      The Surgeons Toolbox: outcomes are only as good as the contents...
      The field of surgical research has been growing rapidly with the development of new surgical and disease models. An extremely important and often overlooked aspect of creating these models is...
      Speaker: Szczepan Baran, VMD, MS
      Presented at: Laboratory Animal Sciences Virtual Event Series 2015
      4
      NOV 12, 2014 | 8:00 AM
      Analytical Ultracentrifugation of Carbon Nanotubes
      Analytical Ultracentrifugation of Carbon Nanotubes
          Sedimentation velocity (SV) experiments on dispersed nanoparticles are a powerful method for determining otherwise hard to evaluate properties of the nanoparticle such as the...
      Speaker: Jeffrey A Fagan, PhD
      Presented at: Beckman Coulter Life Sciences Virtual Trade Show
      Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
      28
      OCT 30, 2014 | 6:00 AM
      A platform for combining the results of WES and RNA-seq analysis
      A platform for combining the results of WES and RNA-seq analysis
      Cancer research is being revolutionized by targeted gene panels, whole exome sequencing (WES), whole genome sequencing (WGS), and transcriptome sequencing (RNA-Seq).Many analyses, such as...
      Speaker: Naomi Thomson
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
      12
      OCT 29, 2014 | 3:00 PM
      Drug-tailored, efficacy-predictive and prognostic biomarker approaches for proper patient selection
      Drug-tailored, efficacy-predictive and prognostic biomarker approaches...
      Over the last decade we have witnessed tremendous advances in our understanding of the underlying molecular alterations in human cancer. This has stimulated excitement for our ability to deve...
      Speaker: Peter Blume-Jensen, MD, PhD
      Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
      0
      SEP 16, 2014 | 9:30 AM
      Analysis of Oncomine Knowledgebase to identify NFE2L2 pathway as a novel therapeutic opportunity in multiple cancer types
      Analysis of Oncomine Knowledgebase to identify NFE2L2 pathway...
      Late-stage drug attrition rates in oncology remain higher than in other therapeutic areas. To reduce attrition, it is critical to identify appropriate drug targets and pre-clinical models. Th...
      Speaker: Nickolay Khazanov, PhD
      Presented at: Advanced Genomics in Clinical and Translational Research - Informing end-to-end cancer drug development with bioinformatics
      18
      AUG 21, 2014 | 8:45 AM
      From Genetics to Genetic Medicine: Gene Therapy for vision restoration as a case study
      From Genetics to Genetic Medicine: Gene Therapy for vision restoration...
      Gene therapy for two forms of inherited retinal degeneration have met promising safety and efficacy endpoints in early stage clinical trials. These approaches made use of a replication defect...
      Speaker: Luk H Vandenberghe, PhD
      Presented at: Genetics and Genomics Virtual Event Series 2014
      0
      JUL 10, 2014 | 9:00 AM
      More Predictive Results in Cell-Based Assays
      More Predictive Results in Cell-Based Assays
       In this webinar, our expert panel of speakers will introduce the new EnSight™ Multimode Plate Reader from PerkinElmer and demonstrate how the unique combination of well-imagin...
      Speaker: Dr. Philip Gribbon, Dr. Volker Eckelt, Dr. Norbert Garbow
      Revvity
      18
      JUL 10, 2014 | 1:00 AM
      More Predictive Results in Cell-Based Assays
      More Predictive Results in Cell-Based Assays
      In this webinar, our expert panel of speakers will introduce the new EnSight™ Multimode Plate Reader from PerkinElmer and demonstrate how the unique combination of well-imaging with lab...
      Speaker: Dr. Philip Gribbon, Dr. Volker Eckelt, Dr. Norbert Garbow
      Sponsored By: Revvity
      17
      MAY 30, 2014 | 3:00 PM
      Opportunities to simplify clinical lipid assessment
      Opportunities to simplify clinical lipid assessment
      Laboratory assessment of serum lipid and lipoprotein levels is essential for the management of the risk of atherothrombotic cardiovascular disease (CVD). Traditionally, this has involved the...
      Speaker: David R Sullivan, MBBS, FRACP, FRCPA
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2014
      36
      MAY 30, 2014 | 3:00 PM
      PoCT Integrated into Clinical Care Contributing to Improved Cardiac Outcomes in Rural Areas
      PoCT Integrated into Clinical Care Contributing to Improved Cardiac...
      Clinical networks link groups of professionals and organisations from primary, secondary and tertiary care, shifting emphasis from institutional to patient needs and outcomes. One of the main...
      Speaker: Rosy Tirimacco BSc
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2014
      50
      MAY 30, 2014 | 11:30 AM
      Keynote: Biochemical Markers of Bone Turnover in the Management of Postmenopausal Osteoporosis
      Keynote: Biochemical Markers of Bone Turnover in the Management...
      Bone turnover markers (BTM) are biological markers of bone remodeling (turnover). The entire skeleton is replaced every 10 years by remodeling, a process constantly going on at different phas...
      Speaker: Paul D Miller, MD
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2014
      59
    NOV 11, 2015 | 6:00 AM
    A Comprehensive Toxicology Screening Solution Targeted to Individual Needs
    A Comprehensive Toxicology Screening Solution Targeted to Individual...
    Drugs of abuse toxicology testing by immunoassays is widely used clinically, but also known to be inadequate for many clinical indications. In this presentation, the speaker will outline diff...
    Speaker: Ping Wang, PhD, DABCC, FACB
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    3
    NOV 11, 2015 | 6:00 AM
    Next-generation sequencing for BRCA1 and BRCA2 mutation testing
    Next-generation sequencing for BRCA1 and BRCA2 mutation testing
    Cancer is complex, but recent findings are yielding a greater understanding of the disease. The tumor suppressor genes BRCA1 and BRCA2 are implicated in breast, ovarian, prostate, and other c...
    Speaker: Raed Samara, PhD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2015
    2
    NOV 10, 2015 | 8:00 AM
    WEBINAR: Analysis of Chemical Contaminants in Drinking Water: Two techniques to analyze pesticides and disinfection products in drinking water in less time
    WEBINAR: Analysis of Chemical Contaminants in Drinking Water:...
    Date: Nov 10, 2015 8AM Pacific, 11AM Eastern, 4PM GMT Transporting smaller volumes of a sample, injecting less matrix, examining lower limits of detection and staying ahead of government r...
    Speaker: Jonathan Beck, PhD
    Sponsored By: Thermo Fisher Scientific, Thermo Fisher Scientific
    6 1
    OCT 01, 2015 | 12:00 PM
    Can the World's First Animal Study Software to Emulate Clinical Trial Approaches Improve Clinical Outcome Predictability?
    Can the World's First Animal Study Software to Emulate Clinical...
    The 95% failure rate of anticancer therapeutic being approved after positive Phase III trials suggests that existing pre-clinical approaches and tools are inadequate predictors of clinical ou...
    Speaker: Eric Ibsen
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    3
    OCT 01, 2015 | 12:00 PM
    Detection and sequencing of rare mutations using mutation enrichment via COLD-PCR
    Detection and sequencing of rare mutations using mutation enrichment...
    Detection of rare mutations is required for reliable identification of mutations in liquid biopsies and heterogeneous tumors. Yet low-level mutation detection remains a technically challengin...
    Speaker: Mike Makrigiorgos, PhD, DABMP
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    2
     
     
  • SEP 30, 2015 | 7:30 AM
    Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
    Ligand-Targeted Therapies for Cancer and Autoimmune Diseases
    We have been developing methods to target drugs specifically to pathologic cells, thereby avoiding collateral toxicity to healthy cells. In the case of cancer, we have exploited up-regulation...
    Speaker: Philip Low, PhD
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2015
    2
    JUL 22, 2015 | 9:00 AM
    WEBINAR: Investigative in vitro Drug Toxicology with Human iPSC-derived Cardiomyocytes
    WEBINAR: Investigative in vitro Drug Toxicology with Human iPSC-derived...
    DATE: July 22, 2015TIME: 9:00AM PT, 12:00PM ETHuman induced pluripotent stem cells (iPSCs) bring human biology into pre-clinical aspects of drug discovery. iPSC-derived cardiomyocytes have em...
    Speaker: Blake Anson, PhD , Mathew Brock, PhD
    Sponsored By: Cellular Dynamics, Cellular Dynamics
    10 1
    MAY 26, 2015 | 6:00 AM
    Cell-Imaging and Luminescence Based Cytotoxicity Analysis using the EnSight Multimode Plate Reader
    Cell-Imaging and Luminescence Based Cytotoxicity Analysis using...
    Sign up here to watch this Webinar On DemandCytotoxicity remains one of the major causes of drug withdrawal and there is an urgent need for reliable and time-saving assay workflows. Cells exp...
    Speaker: Philip Gribbon , Frauke Haenel, PhD
    Sponsored By: Revvity
    24 1
    APR 30, 2015 | 8:00 AM
    Swimming in Transcriptomics: Using Next-Gen sequencing to study the evolution of neural circuitry and swimming behavior of sea slugs
    Swimming in Transcriptomics: Using Next-Gen sequencing to study...
    Sea slugs (Mollusca, Gastropoda, Nudipleura) provide unique opportunities for studying the evolution of behavior at the levels of single neurons, neural circuits, and their synaptic connectio...
    Speaker: Adriano Senatore, PhD, MSc, BSc
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Genomics, Beckman Coulter Genomics
    9
    MAR 19, 2015 | 7:30 AM
    Keynote - The Environmental Causes of Schizophrenia - Developmental Hazards, Social Defeat, and Drug Abuse
    Keynote - The Environmental Causes of Schizophrenia - Developmental...
    Traditional psychiatric textbooks describe schizophrenia as a clinical enigma of unknown aetiology. However, this is no longer true. We now know a great deal about the risk factors, or contr...
    Speaker: Sir Robin Murray, PhD
    Presented at: Neuroscience Virtual Event Series 2015
    13
     
     
  • FEB 10, 2015 | 12:00 PM
    Primary HPV Cervical Cancer Screening: Supporting data and guidance updates
    Primary HPV Cervical Cancer Screening: Supporting data and guidance...
    Date: Tuesday, February 10th, 2015Time: 12:00PM PST, 3:00PM ESTFor the last 60 years the primary method of preventing cervical cancer in both the U.S. and Europe has been cervical cytology ...
    Speaker: Julia Engstrom-Melnyk, PhD
    Sponsored By: Roche Molecular Diagnostics, Roche Molecular Diagnostics
    8 5
    FEB 10, 2015 | 9:00 AM
    Primary HPV Cervical Cancer Screening: Supporting data and guidance updates
    Primary HPV Cervical Cancer Screening: Supporting data and guidance...
    Date: Tuesday, February 10th, 2015Time: 9:00AM PST, 12:00PM ESTFor the last 60 years the primary method of preventing cervical cancer in both the U.S. and Europe has been cervical cytology ...
    Speaker: Julia Engstrom-Melnyk, PhD
    Sponsored By: Roche Molecular Diagnostics, Roche Molecular Diagnostics
    10 24
    FEB 05, 2015 | 7:30 AM
    C.E. CREDITS
    Small Animal Anesthesia: A New Method to Help Control Variables
    Small Animal Anesthesia: A New Method to Help Control Variables
    An anesthesia system designed to accommodate the physiological characteristics of small animals, including rats and mice, has the potential to provide great practical value to the life scienc...
    Speaker: Andrew H Ide, BS
    Presented at: Laboratory Animal Sciences Virtual Event Series 2015
    12
    FEB 04, 2015 | 1:30 PM
    C.E. CREDITS
    The Surgeons Toolbox: outcomes are only as good as the contents of the box
    The Surgeons Toolbox: outcomes are only as good as the contents...
    The field of surgical research has been growing rapidly with the development of new surgical and disease models. An extremely important and often overlooked aspect of creating these models is...
    Speaker: Szczepan Baran, VMD, MS
    Presented at: Laboratory Animal Sciences Virtual Event Series 2015
    4
    NOV 12, 2014 | 8:00 AM
    Analytical Ultracentrifugation of Carbon Nanotubes
    Analytical Ultracentrifugation of Carbon Nanotubes
        Sedimentation velocity (SV) experiments on dispersed nanoparticles are a powerful method for determining otherwise hard to evaluate properties of the nanoparticle such as the...
    Speaker: Jeffrey A Fagan, PhD
    Presented at: Beckman Coulter Life Sciences Virtual Trade Show
    Sponsored By: Beckman Coulter Life Sciences, Beckman Coulter Life Sciences
    28
     
     
  • OCT 30, 2014 | 6:00 AM
    A platform for combining the results of WES and RNA-seq analysis
    A platform for combining the results of WES and RNA-seq analysis
    Cancer research is being revolutionized by targeted gene panels, whole exome sequencing (WES), whole genome sequencing (WGS), and transcriptome sequencing (RNA-Seq).Many analyses, such as...
    Speaker: Naomi Thomson
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
    12
    OCT 29, 2014 | 3:00 PM
    Drug-tailored, efficacy-predictive and prognostic biomarker approaches for proper patient selection
    Drug-tailored, efficacy-predictive and prognostic biomarker approaches...
    Over the last decade we have witnessed tremendous advances in our understanding of the underlying molecular alterations in human cancer. This has stimulated excitement for our ability to deve...
    Speaker: Peter Blume-Jensen, MD, PhD
    Presented at: Cancer: Research, Discovery and Therapeutics Virtual Event Series 2014
    0
    SEP 16, 2014 | 9:30 AM
    Analysis of Oncomine Knowledgebase to identify NFE2L2 pathway as a novel therapeutic opportunity in multiple cancer types
    Analysis of Oncomine Knowledgebase to identify NFE2L2 pathway...
    Late-stage drug attrition rates in oncology remain higher than in other therapeutic areas. To reduce attrition, it is critical to identify appropriate drug targets and pre-clinical models. Th...
    Speaker: Nickolay Khazanov, PhD
    Presented at: Advanced Genomics in Clinical and Translational Research - Informing end-to-end cancer drug development with bioinformatics
    18
    AUG 21, 2014 | 8:45 AM
    From Genetics to Genetic Medicine: Gene Therapy for vision restoration as a case study
    From Genetics to Genetic Medicine: Gene Therapy for vision restoration...
    Gene therapy for two forms of inherited retinal degeneration have met promising safety and efficacy endpoints in early stage clinical trials. These approaches made use of a replication defect...
    Speaker: Luk H Vandenberghe, PhD
    Presented at: Genetics and Genomics Virtual Event Series 2014
    0
    JUL 10, 2014 | 9:00 AM
    More Predictive Results in Cell-Based Assays
    More Predictive Results in Cell-Based Assays
     In this webinar, our expert panel of speakers will introduce the new EnSight™ Multimode Plate Reader from PerkinElmer and demonstrate how the unique combination of well-imagin...
    Speaker: Dr. Philip Gribbon, Dr. Volker Eckelt, Dr. Norbert Garbow
    Revvity
    18
     
     
  • JUL 10, 2014 | 1:00 AM
    More Predictive Results in Cell-Based Assays
    More Predictive Results in Cell-Based Assays
    In this webinar, our expert panel of speakers will introduce the new EnSight™ Multimode Plate Reader from PerkinElmer and demonstrate how the unique combination of well-imaging with lab...
    Speaker: Dr. Philip Gribbon, Dr. Volker Eckelt, Dr. Norbert Garbow
    Sponsored By: Revvity
    17
    MAY 30, 2014 | 3:00 PM
    Opportunities to simplify clinical lipid assessment
    Opportunities to simplify clinical lipid assessment
    Laboratory assessment of serum lipid and lipoprotein levels is essential for the management of the risk of atherothrombotic cardiovascular disease (CVD). Traditionally, this has involved the...
    Speaker: David R Sullivan, MBBS, FRACP, FRCPA
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2014
    36
    MAY 30, 2014 | 3:00 PM
    PoCT Integrated into Clinical Care Contributing to Improved Cardiac Outcomes in Rural Areas
    PoCT Integrated into Clinical Care Contributing to Improved Cardiac...
    Clinical networks link groups of professionals and organisations from primary, secondary and tertiary care, shifting emphasis from institutional to patient needs and outcomes. One of the main...
    Speaker: Rosy Tirimacco BSc
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2014
    50
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    Keynote: Biochemical Markers of Bone Turnover in the Management of Postmenopausal Osteoporosis
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    Bone turnover markers (BTM) are biological markers of bone remodeling (turnover). The entire skeleton is replaced every 10 years by remodeling, a process constantly going on at different phas...
    Speaker: Paul D Miller, MD
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2014
    59
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