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    CHO Cells

    Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics.

    Webinars

    • MAR 28, 2019 | 12:00 PM
      The Intersection of Nutrition, Microbiome and Medical Cannabis
      The Intersection of Nutrition, Microbiome and Medical Cannabis
      Whole food plant-based diets and medical cannabis have shared and continues to share similar levels of scrutiny, doubt, and stigma by the traditional medical community. One reason for the med...
      Speaker: Debra Kimless, MD
      Presented at: Cannabis Sciences Virtual Event Series 2019
      6
      MAR 26, 2019 | 2:00 PM
      The Role of Endosomes in Prostate Cancer
      The Role of Endosomes in Prostate Cancer
      DATE:  March 26, 2019TIME:  2:00pm PDT, 5:00pm EDT  Prostate cancer is the second most common form of cancer in males, affecting one in eight men by the time t...
      Speaker: Ian Johnson, BSc., Ph.D
      Sponsored By: Thermo Fisher Scientific/Gibco
      7
      MAR 20, 2019 | 9:00 AM
      Getting started in 3D - Setting up your lab and reviewing common pitfalls of researchers new to 3D
      Getting started in 3D - Setting up your lab and reviewing common...
      DATE:  March 20, 2019TIME:  9:00am PDT...
      Speaker: Mark Kennedy, Ph.D.
      Sponsored By: Thermo Fisher Scientific
      7
      FEB 20, 2019 | 9:00 AM
      Choosing the best system to answer your question - What question are you looking to answer?
      Choosing the best system to answer your question - What question...
      DATE:  February 20, 2019TIME:  9:00am PST  ...
      Speaker: David Kuninger, PhD
      Sponsored By: Thermo Fisher Scientific
      15 1
      JAN 31, 2019 | 1:30 PM
      Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications
      Standardization of Cell Viability Assays in Primary Cells as a...
      Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and ne...
      Speaker: Simon Kaja, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      2
      JAN 31, 2019 | 12:00 PM
      From Start to Finish, Bio-Techne Solutions for Bioprocessing
      From Start to Finish, Bio-Techne Solutions for Bioprocessing
      As the pace of biotherapeutic approvals increases, so does the pressure on makers of new biologics to complete their development processes faster and more efficiently. The success of the drug...
      Speaker: Christopher Heger, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      Sponsored By: ACD - A Bio-Techne Brand
      3 1
      JAN 31, 2019 | 10:30 AM
      Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications
      Overcoming Limitations of Conventional Tag Systems - Strep-Tactin®...
      The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity a...
      Speaker: Dennis Karthaus, MS
      Presented at: BioProcessing Virtual Event Series 2019
      2
      JAN 31, 2019 | 10:30 AM
      Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries to Improve Process Efficiencies
      Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries...
      Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-t...
      Speaker: Buzz Lobbezoo
      Presented at: BioProcessing Virtual Event Series 2019
      Sponsored By: Astrea Bioseparations
      1
      JAN 31, 2019 | 9:00 AM
      Streamlining the Biologics Development Process: From Transient to Stable Production in the ExpiCHO System
      Streamlining the Biologics Development Process: From Transient...
      The biologics development process is fraught with risks from pre-clinical studies through clinical evaluation. Prominent among these risks are changes in the critical quality attributes of th...
      Speaker: Jon Zmuda, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      1
      JAN 31, 2019 | 7:30 AM
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
      Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
      Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
      Speaker: A.C. Matin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      1
      JAN 31, 2019 | 6:00 AM
      Overcoming Challenges in EuCODE Cell Culture Platform Development
      Overcoming Challenges in EuCODE Cell Culture Platform Development
      Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the...
      Speaker: Weimin Lin, PhD
      Presented at: BioProcessing Virtual Event Series 2019
      3
      JAN 15, 2019 | 12:00 AM
      Flexible manufacturing strategies for cell and gene therapy products
      Flexible manufacturing strategies for cell and gene therapy products
      DATE: January 15, 2018TIME:  4:00pm SGTPThere is a clear call to automate manufacturing for cell and gene therapy products, but when should you automate? In this session, we...
      Speaker: Wenyan Leong, PhD
      Sponsored By: TERUMO BCT
      6
      DEC 05, 2018 | 8:00 PM
      The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary Tumors and in Circulation
      The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary...
      DATE: December 05, 2018TIME:  08:00 PSTWhat is desperately needed are methods to detect cells that cause metastasis–known as cancer stem cells (CSC), a subpopulation of...
      Speaker: Bruno Sainz, PhD
      Sponsored By: Thermo Fisher Scientific
      5 3
      DEC 05, 2018 | 7:00 AM
      The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary Tumors and in Circulation
      The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary...
      DATE: December 05, 2018TIME:  07:00 PSTWhat is desperately needed are methods to detect cells that cause metastasis–known as cancer stem cells (CSC), a subpopulation of...
      Speaker: Bruno Sainz, PhD
      Sponsored By: Thermo Fisher Scientific
      7
      DEC 03, 2018 | 11:00 PM
      Pluripotent stem cells: How to raise the quality of your PSC culture
      Pluripotent stem cells: How to raise the quality of your PSC culture
      DATE:    December 4, 2018TIME:    8:00am CET PSCs represent an important tool in a wide range of applications, including basic research, disease...
      Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
      Sponsored By: Eppendorf
      5
      NOV 13, 2018 | 6:00 AM
      From cell seeding to analysis - Getting the best out of your cell-based assay
      From cell seeding to analysis - Getting the best out of your cell-based...
      DATE: November 13,2018TIME:  3:00pm CET, 7:30pm IST, 06:00am PST The assessment of cell health and cellular responses after experimental manipulation continue to be a very important...
      Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
      Sponsored By: Eppendorf
      8
      MAY 17, 2018 | 10:00 AM
      A comprehensive workflow solution from gene synthesis to purified proteins
      A comprehensive workflow solution from gene synthesis to purified...
      Reliable expression of recombinant proteins in human or CHO cells is essential for many aspects of biomedical research and drug development but is often hampered by low expression yields that...
      Speaker: Michael Liss, PhD
      Presented at: Gibco ExpressionWorld 2018
      5
      MAY 17, 2018 | 6:00 AM
      Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems
      Strategies for High-Titer Protein Expression Using the ExpiCHO...
      The ExpiCHO and Expi293 transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate d...
      Speaker: Jon Zmuda, PhD
      Presented at: Gibco ExpressionWorld 2018
      6
      MAY 17, 2018 | 5:00 AM
      Accelerating Bio-Production Using ExpiCHO Stable Production Medium
      Accelerating Bio-Production Using ExpiCHO Stable Production Medium
      Many research laboratories use the ExpiCHO Expression System to transiently produce candidate biomolecules for biologics development. However, after the lead candidate is found, a stable cell...
      Speaker: Shreya Lowmaster, MS
      Presented at: Gibco ExpressionWorld 2018
      7
      MAY 16, 2018 | 10:00 PM
      Basic concepts in transient expression using 293 and CHO cells (presented in Chinese)
      Basic concepts in transient expression using 293 and CHO cells...
      HEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系,可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料,以及使用293或CHO细胞时需要考虑的主要差异。...
      Speaker: Jian Liu
      Presented at: Gibco ExpressionWorld 2018
      5
      MAY 16, 2018 | 8:00 PM
      ExpiCHO High-Efficiency Protein Expression System: Ultra-high Titers Comparable to Stable Transfection Systems Achieved in Two Weeks (Presented in Chinese)
      ExpiCHO High-Efficiency Protein Expression System: Ultra-high...
      ExpiCHO是一种高效的瞬时表达系统,可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用,获得CHO表达蛋白必须构建稳定的细胞株,这一过程不仅耗时耗力,而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白,往往只能采用293细胞的瞬转表达系统,但该系统产量低,表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...
      Speaker: Yuedan Kong
      Presented at: Gibco ExpressionWorld 2018
      7
      MAY 16, 2018 | 7:00 PM
      Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems (presented in Chinese)
      Strategies for High-Titer Protein Expression Using the ExpiCHO...
      Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统,目前已广泛应用于治疗性候选药物的早期鉴定,诊断性试剂的快速生产,以及疫苗研发、结构生物学和膜蛋白生物学等研究领域,成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达,但HEK293 细胞和 CHO 细胞之间存在的内在...
      Speaker: Chao Yan Liu
      Presented at: Gibco ExpressionWorld 2018
      6 2
      FEB 01, 2018 | 7:00 PM
      In vitro research method for screening inhibitors of protein translation
      In vitro research method for screening inhibitors of protein translation
      In vitro translation (IVT), or cell-free expression, offers a unique and powerful research tool to screen for translational inhibitors that regulate both cellular and viral protein expression...
      Speaker: Krishna Vattem, Ph.D.
      Presented at: Gibco ExpressionWorld 2018
      2
      AUG 29, 2017 | 9:00 AM
      Automated Optimization of IgG Production in CHO Cells
      Automated Optimization of IgG Production in CHO Cells
      DATE: August 29th, 2017TIME:  9:00am PT, 12:00pm ET Antibodies are frequently produced from a cell line that has been screened to ensure the protein is expressed with th...
      Speaker: Mike Kowalski, Ph.D. , David Apiyo
      Sponsored By: Biologics by Danaher, Biologics by Danaher
      4
    MAR 28, 2019 | 12:00 PM
    The Intersection of Nutrition, Microbiome and Medical Cannabis
    The Intersection of Nutrition, Microbiome and Medical Cannabis
    Whole food plant-based diets and medical cannabis have shared and continues to share similar levels of scrutiny, doubt, and stigma by the traditional medical community. One reason for the med...
    Speaker: Debra Kimless, MD
    Presented at: Cannabis Sciences Virtual Event Series 2019
    6
    MAR 26, 2019 | 2:00 PM
    The Role of Endosomes in Prostate Cancer
    The Role of Endosomes in Prostate Cancer
    DATE:  March 26, 2019TIME:  2:00pm PDT, 5:00pm EDT  Prostate cancer is the second most common form of cancer in males, affecting one in eight men by the time t...
    Speaker: Ian Johnson, BSc., Ph.D
    Sponsored By: Thermo Fisher Scientific/Gibco
    7
    MAR 20, 2019 | 9:00 AM
    Getting started in 3D - Setting up your lab and reviewing common pitfalls of researchers new to 3D
    Getting started in 3D - Setting up your lab and reviewing common...
    DATE:  March 20, 2019TIME:  9:00am PDT...
    Speaker: Mark Kennedy, Ph.D.
    Sponsored By: Thermo Fisher Scientific
    7
    FEB 20, 2019 | 9:00 AM
    Choosing the best system to answer your question - What question are you looking to answer?
    Choosing the best system to answer your question - What question...
    DATE:  February 20, 2019TIME:  9:00am PST  ...
    Speaker: David Kuninger, PhD
    Sponsored By: Thermo Fisher Scientific
    15 1
    JAN 31, 2019 | 1:30 PM
    Standardization of Cell Viability Assays in Primary Cells as a Prerequisite for Novel Bioprocessing Applications
    Standardization of Cell Viability Assays in Primary Cells as a...
    Development of novel types of therapeutics require adaptation of traditional bioprocessing applications to include the use of primary cells, such as stem cells, fibroblasts, astrocytes and ne...
    Speaker: Simon Kaja, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    2
     
     
  • JAN 31, 2019 | 12:00 PM
    From Start to Finish, Bio-Techne Solutions for Bioprocessing
    From Start to Finish, Bio-Techne Solutions for Bioprocessing
    As the pace of biotherapeutic approvals increases, so does the pressure on makers of new biologics to complete their development processes faster and more efficiently. The success of the drug...
    Speaker: Christopher Heger, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    Sponsored By: ACD - A Bio-Techne Brand
    3 1
    JAN 31, 2019 | 10:30 AM
    Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications
    Overcoming Limitations of Conventional Tag Systems - Strep-Tactin®...
    The Strep-Tactin®XT:Twin-Strep-tag®-purification system enables protein purification at high yields and purity under physiological conditions. Providing the highest binding affinity a...
    Speaker: Dennis Karthaus, MS
    Presented at: BioProcessing Virtual Event Series 2019
    2
    JAN 31, 2019 | 10:30 AM
    Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries to Improve Process Efficiencies
    Flow-Through Polishing and The Use of Multi-Mode Ligand Libraries...
    Multi-mode chromatography has come to prominence in recent years due to the general utility of multi-mode ligands for product capture and polishing. In particular, their application in flow-t...
    Speaker: Buzz Lobbezoo
    Presented at: BioProcessing Virtual Event Series 2019
    Sponsored By: Astrea Bioseparations
    1
    JAN 31, 2019 | 9:00 AM
    Streamlining the Biologics Development Process: From Transient to Stable Production in the ExpiCHO System
    Streamlining the Biologics Development Process: From Transient...
    The biologics development process is fraught with risks from pre-clinical studies through clinical evaluation. Prominent among these risks are changes in the critical quality attributes of th...
    Speaker: Jon Zmuda, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    1
    JAN 31, 2019 | 7:30 AM
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based Gene Delivery for Targeted Treatment of HER2-Positive Breast Cancer in Mice by Prodrugs - A New Gene-Delivered Enzyme Prodrug
    Keynote Presentation: Extracellular Vesicle-Mediated mRNA-Based...
    Prodrugs are harmless in their native state, as they are not targeted by human enzymes. But they can be converted into highly toxic compounds (the “drug”) by viral or bacterial en...
    Speaker: A.C. Matin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    1
     
     
  • JAN 31, 2019 | 6:00 AM
    Overcoming Challenges in EuCODE Cell Culture Platform Development
    Overcoming Challenges in EuCODE Cell Culture Platform Development
    Ambrx’s mammalian expression platform (EuCODE™) enables non-native amino acids (nnAAs) through an expanded genetic code to both generate novel bio-therapeutics and to optimize the...
    Speaker: Weimin Lin, PhD
    Presented at: BioProcessing Virtual Event Series 2019
    3
    JAN 15, 2019 | 12:00 AM
    Flexible manufacturing strategies for cell and gene therapy products
    Flexible manufacturing strategies for cell and gene therapy products
    DATE: January 15, 2018TIME:  4:00pm SGTPThere is a clear call to automate manufacturing for cell and gene therapy products, but when should you automate? In this session, we...
    Speaker: Wenyan Leong, PhD
    Sponsored By: TERUMO BCT
    6
    DEC 05, 2018 | 8:00 PM
    The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary Tumors and in Circulation
    The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary...
    DATE: December 05, 2018TIME:  08:00 PSTWhat is desperately needed are methods to detect cells that cause metastasis–known as cancer stem cells (CSC), a subpopulation of...
    Speaker: Bruno Sainz, PhD
    Sponsored By: Thermo Fisher Scientific
    5 3
    DEC 05, 2018 | 7:00 AM
    The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary Tumors and in Circulation
    The Use of Flow Cytometry to Identify Cancer Stem Cells in Primary...
    DATE: December 05, 2018TIME:  07:00 PSTWhat is desperately needed are methods to detect cells that cause metastasis–known as cancer stem cells (CSC), a subpopulation of...
    Speaker: Bruno Sainz, PhD
    Sponsored By: Thermo Fisher Scientific
    7
    DEC 03, 2018 | 11:00 PM
    Pluripotent stem cells: How to raise the quality of your PSC culture
    Pluripotent stem cells: How to raise the quality of your PSC culture
    DATE:    December 4, 2018TIME:    8:00am CET PSCs represent an important tool in a wide range of applications, including basic research, disease...
    Speaker: Jessica Wagener, PhD , Nadine Mellies, PhD
    Sponsored By: Eppendorf
    5
     
     
  • NOV 13, 2018 | 6:00 AM
    From cell seeding to analysis - Getting the best out of your cell-based assay
    From cell seeding to analysis - Getting the best out of your cell-based...
    DATE: November 13,2018TIME:  3:00pm CET, 7:30pm IST, 06:00am PST The assessment of cell health and cellular responses after experimental manipulation continue to be a very important...
    Speaker: Jessica Wagener, PhD , Erik Bonke, PhD
    Sponsored By: Eppendorf
    8
    MAY 17, 2018 | 10:00 AM
    A comprehensive workflow solution from gene synthesis to purified proteins
    A comprehensive workflow solution from gene synthesis to purified...
    Reliable expression of recombinant proteins in human or CHO cells is essential for many aspects of biomedical research and drug development but is often hampered by low expression yields that...
    Speaker: Michael Liss, PhD
    Presented at: Gibco ExpressionWorld 2018
    5
    MAY 17, 2018 | 6:00 AM
    Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems
    Strategies for High-Titer Protein Expression Using the ExpiCHO...
    The ExpiCHO and Expi293 transient expression systems offer all-in-one solutions for generating high-titer recombinant proteins for a broad range of research applications including candidate d...
    Speaker: Jon Zmuda, PhD
    Presented at: Gibco ExpressionWorld 2018
    6
    MAY 17, 2018 | 5:00 AM
    Accelerating Bio-Production Using ExpiCHO Stable Production Medium
    Accelerating Bio-Production Using ExpiCHO Stable Production Medium
    Many research laboratories use the ExpiCHO Expression System to transiently produce candidate biomolecules for biologics development. However, after the lead candidate is found, a stable cell...
    Speaker: Shreya Lowmaster, MS
    Presented at: Gibco ExpressionWorld 2018
    7
    MAY 16, 2018 | 10:00 PM
    Basic concepts in transient expression using 293 and CHO cells (presented in Chinese)
    Basic concepts in transient expression using 293 and CHO cells...
    HEK293和CHO细胞是快速、规模可调的悬浮培养系统中常用的细胞系,可用于瞬时蛋白表达。此次演讲介绍了瞬时表达的基本概念和使用材料,以及使用293或CHO细胞时需要考虑的主要差异。...
    Speaker: Jian Liu
    Presented at: Gibco ExpressionWorld 2018
    5
     
     
  • MAY 16, 2018 | 8:00 PM
    ExpiCHO High-Efficiency Protein Expression System: Ultra-high Titers Comparable to Stable Transfection Systems Achieved in Two Weeks (Presented in Chinese)
    ExpiCHO High-Efficiency Protein Expression System: Ultra-high...
    ExpiCHO是一种高效的瞬时表达系统,可以在2周内获得与稳定细胞株相当的表达量。 CHO细胞长期以来一直在工业界广泛使用,获得CHO表达蛋白必须构建稳定的细胞株,这一过程不仅耗时耗力,而且需要经验丰富的研发人员。在药物开发阶段需要在较短时间获得大量蛋白,往往只能采用293细胞的瞬转表达系统,但该系统产量低,表达蛋白与CHO系统存在质量差异。ExpiCHO作为高效的瞬时表达系统能...
    Speaker: Yuedan Kong
    Presented at: Gibco ExpressionWorld 2018
    7
    MAY 16, 2018 | 7:00 PM
    Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems (presented in Chinese)
    Strategies for High-Titer Protein Expression Using the ExpiCHO...
    Gibco™ ExpiCHO 和Expi293系统是两款全新优化的瞬时重组蛋白高表达系统,目前已广泛应用于治疗性候选药物的早期鉴定,诊断性试剂的快速生产,以及疫苗研发、结构生物学和膜蛋白生物学等研究领域,成为可以在瞬转中获得极高的蛋白产量的一站式有效途径和工具。虽然多种重组蛋白均可在这两款瞬时表达系统中获得高水平表达,但HEK293 细胞和 CHO 细胞之间存在的内在...
    Speaker: Chao Yan Liu
    Presented at: Gibco ExpressionWorld 2018
    6 2
    FEB 01, 2018 | 7:00 PM
    In vitro research method for screening inhibitors of protein translation
    In vitro research method for screening inhibitors of protein translation
    In vitro translation (IVT), or cell-free expression, offers a unique and powerful research tool to screen for translational inhibitors that regulate both cellular and viral protein expression...
    Speaker: Krishna Vattem, Ph.D.
    Presented at: Gibco ExpressionWorld 2018
    2
    AUG 29, 2017 | 9:00 AM
    Automated Optimization of IgG Production in CHO Cells
    Automated Optimization of IgG Production in CHO Cells
    DATE: August 29th, 2017TIME:  9:00am PT, 12:00pm ET Antibodies are frequently produced from a cell line that has been screened to ensure the protein is expressed with th...
    Speaker: Mike Kowalski, Ph.D. , David Apiyo
    Sponsored By: Biologics by Danaher, Biologics by Danaher
    4
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