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    Method Validation

    Method Validation: the process used to assure the analytical procedure deployed for a specific test is suitable for intended usage

    Webinars

    • DEC 11, 2020 | 7:00 AM
      Solving Confusion with Regulations, QC Design, and Troubleshooting for SARS CoV-2  Assays
      Solving Confusion with Regulations, QC Design, and Troubleshooting...
      Date: November 16, 2020 Time: 7:00am (PST), 10:00am (EST) Join us for a webinar to learn about quality control (QC) regulations, setting the proper QC strategy/design, and troubleshoot SARs...
      Speaker: Sten Westgard, MS , Sharon Ehrmeyer, PhD, MT (ASCP)
      Sponsored By: Technopath Clinical Diagnostics USA
      44 14
      NOV 17, 2020 | 5:00 AM
      Utilizing rapid diagnostics and new methods to quickly diagnose sepsis
      Utilizing rapid diagnostics and new methods to quickly diagnose...
      Date: November 17, 2020 Time: 5:00am (PST), 8:00am (EST), 2:00pm (CET) Septicemia is a serious clinical condition with roundabout 123,000 cases of sepsis a year in England. In recent years t...
      Speaker: Francis Yongblah
      Sponsored By: Bruker Microbiology & Infection Diagnostics
      7
      NOV 12, 2020 | 6:00 AM
      Standardization with Clinical Digital Pathology - The Ki-67 Case
      Standardization with Clinical Digital Pathology - The Ki-67 Case
      The nuclear proliferation biomarker Ki-67 has multiple potential roles in breast and other cancers, both as a prognostic but also predictive marker. However, several studies have shown that...
      Speaker: Dzenita Omanovic
      Presented at: Clinical Diagnostics & Research Virtual Event Series 2020
      Sponsored By: Visiopharm®
      1
      NOV 12, 2020 | 12:00 AM
      Principles of Effective Method Development in Pathobiology Research
      Principles of Effective Method Development in Pathobiology Research
      Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
      Speaker: Vivian Barry, MS
      Presented at: DISCOVERY Global User Symposium
      2
      NOV 05, 2020 | 9:00 AM
      Cell-based Assays to Functionally Characterize Immunotherapy and the Immune Response to Viral Infection
      Cell-based Assays to Functionally Characterize Immunotherapy and...
      DATE: November 5, 2020 TIME: 9:00am (PDT), 12:00pm (EDT) With accelerating development and approval of cell therapies, biologic drugs, and antiviral treatments comes a need for more robust a...
      Speaker: Chris Langsdorf
      Sponsored By: Thermo Fisher Scientific
      8 1
      OCT 21, 2020 | 12:00 AM
      Overcoming Challenges Analyzing Heavy Metals in Cannabis by ICP-MS
      Overcoming Challenges Analyzing Heavy Metals in Cannabis by ICP-MS
      There are many obstacles to overcome when analyzing Heavy Metals in Cannabis by ICPMS. Cannabis matrices can be difficult to prepare and analyze due to the complex components of products tha...
      Speaker: Shianna Hines
      Presented at: Analytical Chemistry & Separation Sciences Virtual Event Series 2020
      1
      OCT 08, 2020 | 10:30 AM
      Keynote Presentation: Principles of Effective Method Development in Pathobiology Research.
      Keynote Presentation: Principles of Effective Method Development...
      Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
      Speaker: Vivian Barry, MS
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
      Sponsored By: Roche Diagnostics
      1
      SEP 30, 2020 | 12:00 PM
      Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
      Efficient Generation of Gene-edited Mouse Models and Cell Lines...
      The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
      Speaker: Gurpreet Balrey, PhD , Peter Romanienko, PhD
      Presented at: CRISPR Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      4
      SEP 29, 2020 | 8:00 AM
      Microbial Quality Control in Manufacturing of Medicinal Products
      Microbial Quality Control in Manufacturing of Medicinal Products
      DATE: September 29, 2020 TIME: 8:00 am PDT There is a steady increase in FDA 483s related to microbiology. Though most are for sterile products, non-sterile and terminally sterilized product...
      Speaker: Ziva Abraham
      Sponsored By: Sartorius
      19 5
      SEP 29, 2020 | 7:00 AM
      Considerations in the Use of Analytical Ultracentrifugation for Characterization of AAV Gene Delivery Vectors
      Considerations in the Use of Analytical Ultracentrifugation for...
      Date: September 29, 2020 Time: 10:00am PTD, 1:00pm EDT alytical Ultracentrifugation (AUC) in the biopharmaceutical industry has traditionally been employed in the analysis of aggregation and...
      Speaker: Christopher Sucato
      Sponsored By: Beckman Coulter Life Sciences
      6 1
      SEP 23, 2020 | 4:30 PM
      Anatomy of a nucleic acid sample preparation
      Anatomy of a nucleic acid sample preparation
      There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “proces...
      Speaker: Katherine Mechling
      Presented at: Cell Biology Virtual Event Series 2020
      Sponsored By: MilliporeSigma
      2 1
      SEP 17, 2020 | 3:30 PM
      SARS-CoV-2 Testing Today and a New Serology Approach For High Throughput Screening
      SARS-CoV-2 Testing Today and a New Serology Approach For High...
      Richard Hughes: New immunogenicity strategies to meet the needs of a developing pandemic Shortly after the COVID-19 pandemic began, the subject of serological testing for anti-viral antibodi...
      Speaker: Russell Garlick, PhD , Richard Hughes , Joel Lefferts, PhD, HCLD, DABCC
      Presented at: Coronavirus Series
      Sponsored By: LGC Seracare
      1
      SEP 17, 2020 | 9:00 AM
      Purification of cells from SARS-CoV-2 convalescent samples and potentially infectious materials with fluorescent-activated cell sorting
      Purification of cells from SARS-CoV-2 convalescent samples and...
      In recent months, we have seen a large part of the global scientific community shift to infectious disease research. In response, researchers are exploring safer and easier methods to handle...
      Speaker: Joe Ostrand, Ph.D.
      Presented at: Coronavirus Series
      Sponsored By: Miltenyi Biotec
      1
      SEP 03, 2020 | 9:00 AM
      The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
      The Complexities of SV Analytical Ultracentrifugation: AAVs are...
      DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
      Speaker: Dr. Lake Paul
      Sponsored By: Beckman Coulter Life Sciences
      12
      AUG 27, 2020 | 7:00 AM
      SARS-CoV-2 Quality Control for Molecular and Serology assays
      SARS-CoV-2 Quality Control for Molecular and Serology assays
      SARS-CoV-2 has created significant challenges for laboratories, requiring the development, validation, and scale up of testing in a very short time period--often this was done for multiple m...
      Speaker: Duane Newton, PhD, D(ABMM), FIDSA , Elitza S. Theel, PhD, D(ABMM)
      Presented at: A Bio-Rad Quality Control Virtual Conference
      Sponsored By: Bio-Rad Laboratories
      20 2
      AUG 27, 2020 | 7:00 AM
      Atlas of the SARS-CoV-2 Proteome
      Atlas of the SARS-CoV-2 Proteome
      DATE: August 27, 2020 TIME: 7:00am PT Novel anti-viral medications, including biologics and small molecule inhibitors, as well as serologic diagnostic tool sets are in urgent demand to fight...
      Speaker: Yuning Chen, Ph.D.
      Sponsored By: Sino Biological
      32 3
      JUL 15, 2020 | 9:00 AM
      Calidad Más Allá de las Cifras
      Calidad Más Allá de las Cifras
      DATE: 15 Julio 2020 TIME: 11:00 COT, 09:00am PDT En esta conferencia tratamos de explorar el concepto profundo, holístico y existencial de la calidad , mucho más allá de...
      Speaker: Esteban Echavarría, MD
      Sponsored By: Abbott
      9 1
      JUL 08, 2020 | 11:00 PM
      Mass Spectrometry Past and Present - Emerging Technologies and Strategies for Quality Management in Today's Clinical Laboratory
      Mass Spectrometry Past and Present - Emerging Technologies and...
      DATE: July 9, 2020 TIME: 2pm SGT, 3pm JST, 4pm AEST Mass Spectrometry (MS) has been an invaluable tool for physicists and basic chemists for over 100 years. It was not until around 30 to 40...
      Speaker: SM Hossein Sadrzadeh, PhD., DABCC, FAACC , Dennis Orton, PhD, FCACB , Jessica Boyd, PhD, FCACB
      Sponsored By: Waters Corporation
      17
      JUL 01, 2020 | 12:45 PM
      Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent Genexus System
      Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent...
      NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
      Speaker: Prof. Dr. Michael Hummel
      Presented at: APJ Virtual Genetic Solutions Tour 2020
      1
      JUN 30, 2020 | 9:00 AM
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
      Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
      DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
      Speaker: Jane Weitzel
      Sponsored By: Sartorius
      17 1
      JUN 30, 2020 | 8:00 AM
      Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex Technology
      Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex...
      DATE: June 30, 2020 TIME: 8:00am PT, 11:00am Understanding the tumor microenvironment (TME) is essential for the development of effective treatments for cancer patients. Traditional multi...
      Speaker: Chifei Sun
      Sponsored By: Leica Biosystems
      19
      JUN 25, 2020 | 8:00 AM
      Mass Spectrometry Past and Present - Emerging Technologies and Strategies for Quality Management in Today's Clinical Laboratory
      Mass Spectrometry Past and Present - Emerging Technologies and...
      DATE: June 25, 2020 TIME: 8:00am PDT, 11:00am EDT, 4:00pm BST, 5:00pm CEST Mass Spectrometry (MS) has been an invaluable tool for physicists and basic chemists for over 100 years. It was not...
      Speaker: SM Hossein Sadrzadeh, PhD., DABCC, FAACC , Dennis Orton, PhD, FCACB , Jessica Boyd, PhD, FCACB
      Sponsored By: Waters Corporation
      34
      JUN 18, 2020 | 7:00 AM
      Want to know if your Cannabis is safe?
      Want to know if your Cannabis is safe?
      Safety and potency testing in the cannabis and hemp industries necessitates analytical systems that include chromatography, mass spectrometry, and real time quantitative polymerase chain rea...
      Speaker: Anthony Macherone, PhD , Timothy Bolduc
      Presented at: Agilent's Virtual Mass Spectacular!
      1
      JUN 09, 2020 | 10:00 AM
      Impacting Patient Outcomes With Rapid Positive Blood Culture ID Utilizing MALDI Mass Spectrometry
      Impacting Patient Outcomes With Rapid Positive Blood Culture ID...
      DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
      Speaker: William (Ike) Northern
      Sponsored By: Bruker Microbiology & Infection Diagnostics
      29
    DEC 11, 2020 | 7:00 AM
    Solving Confusion with Regulations, QC Design, and Troubleshooting for SARS CoV-2  Assays
    Solving Confusion with Regulations, QC Design, and Troubleshooting...
    Date: November 16, 2020 Time: 7:00am (PST), 10:00am (EST) Join us for a webinar to learn about quality control (QC) regulations, setting the proper QC strategy/design, and troubleshoot SARs...
    Speaker: Sten Westgard, MS , Sharon Ehrmeyer, PhD, MT (ASCP)
    Sponsored By: Technopath Clinical Diagnostics USA
    44 14
    NOV 17, 2020 | 5:00 AM
    Utilizing rapid diagnostics and new methods to quickly diagnose sepsis
    Utilizing rapid diagnostics and new methods to quickly diagnose...
    Date: November 17, 2020 Time: 5:00am (PST), 8:00am (EST), 2:00pm (CET) Septicemia is a serious clinical condition with roundabout 123,000 cases of sepsis a year in England. In recent years t...
    Speaker: Francis Yongblah
    Sponsored By: Bruker Microbiology & Infection Diagnostics
    7
    NOV 12, 2020 | 6:00 AM
    Standardization with Clinical Digital Pathology - The Ki-67 Case
    Standardization with Clinical Digital Pathology - The Ki-67 Case
    The nuclear proliferation biomarker Ki-67 has multiple potential roles in breast and other cancers, both as a prognostic but also predictive marker. However, several studies have shown that...
    Speaker: Dzenita Omanovic
    Presented at: Clinical Diagnostics & Research Virtual Event Series 2020
    Sponsored By: Visiopharm®
    1
    NOV 12, 2020 | 12:00 AM
    Principles of Effective Method Development in Pathobiology Research
    Principles of Effective Method Development in Pathobiology Research
    Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
    Speaker: Vivian Barry, MS
    Presented at: DISCOVERY Global User Symposium
    2
    NOV 05, 2020 | 9:00 AM
    Cell-based Assays to Functionally Characterize Immunotherapy and the Immune Response to Viral Infection
    Cell-based Assays to Functionally Characterize Immunotherapy and...
    DATE: November 5, 2020 TIME: 9:00am (PDT), 12:00pm (EDT) With accelerating development and approval of cell therapies, biologic drugs, and antiviral treatments comes a need for more robust a...
    Speaker: Chris Langsdorf
    Sponsored By: Thermo Fisher Scientific
    8 1
     
     
  • OCT 21, 2020 | 12:00 AM
    Overcoming Challenges Analyzing Heavy Metals in Cannabis by ICP-MS
    Overcoming Challenges Analyzing Heavy Metals in Cannabis by ICP-MS
    There are many obstacles to overcome when analyzing Heavy Metals in Cannabis by ICPMS. Cannabis matrices can be difficult to prepare and analyze due to the complex components of products tha...
    Speaker: Shianna Hines
    Presented at: Analytical Chemistry & Separation Sciences Virtual Event Series 2020
    1
    OCT 08, 2020 | 10:30 AM
    Keynote Presentation: Principles of Effective Method Development in Pathobiology Research.
    Keynote Presentation: Principles of Effective Method Development...
    Immunohistochemistry (IHC) is used to determine spatial relationships where we can identify the localization of target proteins in specific regions and cells of a tissue. IHC has been the go...
    Speaker: Vivian Barry, MS
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2020
    Sponsored By: Roche Diagnostics
    1
    SEP 30, 2020 | 12:00 PM
    Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
    Efficient Generation of Gene-edited Mouse Models and Cell Lines...
    The CRISPR/Cas genome editing system has revolutionized nearly every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA,...
    Speaker: Gurpreet Balrey, PhD , Peter Romanienko, PhD
    Presented at: CRISPR Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    4
    SEP 29, 2020 | 8:00 AM
    Microbial Quality Control in Manufacturing of Medicinal Products
    Microbial Quality Control in Manufacturing of Medicinal Products
    DATE: September 29, 2020 TIME: 8:00 am PDT There is a steady increase in FDA 483s related to microbiology. Though most are for sterile products, non-sterile and terminally sterilized product...
    Speaker: Ziva Abraham
    Sponsored By: Sartorius
    19 5
    SEP 29, 2020 | 7:00 AM
    Considerations in the Use of Analytical Ultracentrifugation for Characterization of AAV Gene Delivery Vectors
    Considerations in the Use of Analytical Ultracentrifugation for...
    Date: September 29, 2020 Time: 10:00am PTD, 1:00pm EDT alytical Ultracentrifugation (AUC) in the biopharmaceutical industry has traditionally been employed in the analysis of aggregation and...
    Speaker: Christopher Sucato
    Sponsored By: Beckman Coulter Life Sciences
    6 1
     
     
  • SEP 23, 2020 | 4:30 PM
    Anatomy of a nucleic acid sample preparation
    Anatomy of a nucleic acid sample preparation
    There are many methods of nucleic acid isolation. Each technology offers different approaches of purification of the template. However, it is important to control carry over of “proces...
    Speaker: Katherine Mechling
    Presented at: Cell Biology Virtual Event Series 2020
    Sponsored By: MilliporeSigma
    2 1
    SEP 17, 2020 | 3:30 PM
    SARS-CoV-2 Testing Today and a New Serology Approach For High Throughput Screening
    SARS-CoV-2 Testing Today and a New Serology Approach For High...
    Richard Hughes: New immunogenicity strategies to meet the needs of a developing pandemic Shortly after the COVID-19 pandemic began, the subject of serological testing for anti-viral antibodi...
    Speaker: Russell Garlick, PhD , Richard Hughes , Joel Lefferts, PhD, HCLD, DABCC
    Presented at: Coronavirus Series
    Sponsored By: LGC Seracare
    1
    SEP 17, 2020 | 9:00 AM
    Purification of cells from SARS-CoV-2 convalescent samples and potentially infectious materials with fluorescent-activated cell sorting
    Purification of cells from SARS-CoV-2 convalescent samples and...
    In recent months, we have seen a large part of the global scientific community shift to infectious disease research. In response, researchers are exploring safer and easier methods to handle...
    Speaker: Joe Ostrand, Ph.D.
    Presented at: Coronavirus Series
    Sponsored By: Miltenyi Biotec
    1
    SEP 03, 2020 | 9:00 AM
    The Complexities of SV Analytical Ultracentrifugation: AAVs are not Simple, Binary Systems
    The Complexities of SV Analytical Ultracentrifugation: AAVs are...
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    Speaker: Dr. Lake Paul
    Sponsored By: Beckman Coulter Life Sciences
    12
    AUG 27, 2020 | 7:00 AM
    SARS-CoV-2 Quality Control for Molecular and Serology assays
    SARS-CoV-2 Quality Control for Molecular and Serology assays
    SARS-CoV-2 has created significant challenges for laboratories, requiring the development, validation, and scale up of testing in a very short time period--often this was done for multiple m...
    Speaker: Duane Newton, PhD, D(ABMM), FIDSA , Elitza S. Theel, PhD, D(ABMM)
    Presented at: A Bio-Rad Quality Control Virtual Conference
    Sponsored By: Bio-Rad Laboratories
    20 2
     
     
  • AUG 27, 2020 | 7:00 AM
    Atlas of the SARS-CoV-2 Proteome
    Atlas of the SARS-CoV-2 Proteome
    DATE: August 27, 2020 TIME: 7:00am PT Novel anti-viral medications, including biologics and small molecule inhibitors, as well as serologic diagnostic tool sets are in urgent demand to fight...
    Speaker: Yuning Chen, Ph.D.
    Sponsored By: Sino Biological
    32 3
    JUL 15, 2020 | 9:00 AM
    Calidad Más Allá de las Cifras
    Calidad Más Allá de las Cifras
    DATE: 15 Julio 2020 TIME: 11:00 COT, 09:00am PDT En esta conferencia tratamos de explorar el concepto profundo, holístico y existencial de la calidad , mucho más allá de...
    Speaker: Esteban Echavarría, MD
    Sponsored By: Abbott
    9 1
    JUL 08, 2020 | 11:00 PM
    Mass Spectrometry Past and Present - Emerging Technologies and Strategies for Quality Management in Today's Clinical Laboratory
    Mass Spectrometry Past and Present - Emerging Technologies and...
    DATE: July 9, 2020 TIME: 2pm SGT, 3pm JST, 4pm AEST Mass Spectrometry (MS) has been an invaluable tool for physicists and basic chemists for over 100 years. It was not until around 30 to 40...
    Speaker: SM Hossein Sadrzadeh, PhD., DABCC, FAACC , Dennis Orton, PhD, FCACB , Jessica Boyd, PhD, FCACB
    Sponsored By: Waters Corporation
    17
    JUL 01, 2020 | 12:45 PM
    Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent Genexus System
    Multicentric Evaluation of Oncomine Precision Assay on Ion Torrent...
    NGS is still perceived as a complex, hard to implement and validate method that requires highly experienced people. Outsourcing next-generation sequencing (NGS) testing could be an option to...
    Speaker: Prof. Dr. Michael Hummel
    Presented at: APJ Virtual Genetic Solutions Tour 2020
    1
    JUN 30, 2020 | 9:00 AM
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued Verification, in the Lifecycle of Analytical Procedures
    Quality Control for the Pharmaceutical Industry; Stage 3, Continued...
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Speaker: Jane Weitzel
    Sponsored By: Sartorius
    17 1
     
     
  • JUN 30, 2020 | 8:00 AM
    Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex Technology
    Phenotyping the Tumor Microenvironment Using DNA Barcoded Multiplex...
    DATE: June 30, 2020 TIME: 8:00am PT, 11:00am Understanding the tumor microenvironment (TME) is essential for the development of effective treatments for cancer patients. Traditional multi...
    Speaker: Chifei Sun
    Sponsored By: Leica Biosystems
    19
    JUN 25, 2020 | 8:00 AM
    Mass Spectrometry Past and Present - Emerging Technologies and Strategies for Quality Management in Today's Clinical Laboratory
    Mass Spectrometry Past and Present - Emerging Technologies and...
    DATE: June 25, 2020 TIME: 8:00am PDT, 11:00am EDT, 4:00pm BST, 5:00pm CEST Mass Spectrometry (MS) has been an invaluable tool for physicists and basic chemists for over 100 years. It was not...
    Speaker: SM Hossein Sadrzadeh, PhD., DABCC, FAACC , Dennis Orton, PhD, FCACB , Jessica Boyd, PhD, FCACB
    Sponsored By: Waters Corporation
    34
    JUN 18, 2020 | 7:00 AM
    Want to know if your Cannabis is safe?
    Want to know if your Cannabis is safe?
    Safety and potency testing in the cannabis and hemp industries necessitates analytical systems that include chromatography, mass spectrometry, and real time quantitative polymerase chain rea...
    Speaker: Anthony Macherone, PhD , Timothy Bolduc
    Presented at: Agilent's Virtual Mass Spectacular!
    1
    JUN 09, 2020 | 10:00 AM
    Impacting Patient Outcomes With Rapid Positive Blood Culture ID Utilizing MALDI Mass Spectrometry
    Impacting Patient Outcomes With Rapid Positive Blood Culture ID...
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    Speaker: William (Ike) Northern
    Sponsored By: Bruker Microbiology & Infection Diagnostics
    29
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